Immunoadsorber for specific apheresis of autoantibodies in the treatment of bullous pemphigoid.


:Bullous pemphigoid (BP) is an autoimmune blistering skin disease associated with autoantibodies against two hemidesmosomal proteins, BP180 (type XVII collagen) and BP230. As the pathogenic relevance of antibodies against the immunodominant NC16A domain of BP180 has been clearly demonstrated, specific removal of these antibodies should be a rational therapeutic approach. Here, we evaluated three recombinant forms of bacterially produced BP180 NC16A, a monomer, trimer, and tetramer, together with different matrices for their efficacy to specifically adsorb autoantibodies from BP plasma samples. An adsorber consisting of NC16A-trimer coupled to NHS-activated Sepharose 4 Fast Flow revealed satisfying adsorption rates and a high specificity. The NC16A-trimer adsorber was regenerable and autoclavable. It has the potential to be used for specific immunoadsorption to treat severe and refractory BP and other pemphigoid diseases associated with BP180 NC16A reactivity.


Arch Dermatol Res


Mersmann M,Dworschak J,Ebermann K,Komorowski L,Schlumberger W,Stöcker W,Zillikens D,Probst C,Schmidt E




Has Abstract


2016-01-01 00:00:00














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    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Shaheen MS,Silverberg JI

    更新日期:2020-07-08 00:00:00

  • Lichen planus, chronic liver diseases, and immunologic involvement.

    abstract::We report the clinical features of 62 consecutive patients with lichen planus observed in 18 months. The largest number of cases occurred between 50 and 70 years of age. Thirty-four patients had lichen planus only. In the remainder, lichen planus was associated with chronic liver diseases (16 cases), immune-related di...

    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Cottoni F,Solinas A,Piga MR,Tocco A,Lissia M,Cerimele D

    更新日期:1988-01-01 00:00:00

  • Excessive costimulation of CD3-dependent lymphocyte response by extracellular matrix proteins in severe widespread psoriasis.

    abstract::Costimulation of anti-CD3-triggered proliferative T-cell responses by type I and type IV collagen and fibronectin was studied in 25 patients with psoriasis and 12 healthy subjects. The stimulation index of anti-CD3-mediated responses in the presence of type I collagen was about half that in the controls. Although the ...

    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Glinski W,Gorski A,Glinska-Ferenz M,Majewski S,Stepien-Sopniewska B

    更新日期:1995-01-01 00:00:00

  • A novel point mutation in type III collagen gene resulting in exon 24 skipping in a case of vascular type Ehlers-Danlos syndrome.

    abstract::A novel point mutation in a patient with vascular type Ehlers-Danlos syndrome is herein reported. The patient experienced pneumothorax and multiple arterial troubles during the past 10 years. The patient's hand skin was atrophic, and a paper-like atrophic scar was seen. The collagen microfibrils from the patient's ski...

    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Okamoto O,Ando T,Watanabe A,Sato F,Mimata H,Shimada T,Fujiwara S

    更新日期:2008-10-01 00:00:00

  • Understanding the phenotypic similarities between IFAP and Olmsted syndrome from a molecular perspective: the interaction of MBTPS2 and TRPV3.

    abstract::Ichthyosis Follicularis, Atrichia, and Photophobia (IFAP) is a severe rare genetic disorder caused by mutations in the gene encoding the Membrane-Bound Transcription Factor Peptidase, Site 2 (MBTPS2). Olmsted syndrome is another rare genetic disease with overlapping clinical features caused by mutations in the gene en...

    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Nemer G,Safi R,Kreidieh F,Usta J,Bergqvist C,Ballout F,Btadini W,Hamzeh N,Abbas O,Kibbi AG,Shimomura Y,Kurban M

    更新日期:2017-10-01 00:00:00

  • The rhino mouse model: the effects of topically applied all-trans retinoic acid and CD271 on the fine structure of the epidermis and utricle wall of pseudocomedones.

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    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Bernerd F,Ortonne JP,Bouclier M,Chatelus A,Hensby C

    更新日期:1991-01-01 00:00:00

  • Stimulation of T cells by autologous mononuclear leukocytes and epidermal cells in psoriasis.

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    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Schopf RE,Hoffmann A,Jung M,Morsches B,Bork K

    更新日期:1986-01-01 00:00:00

  • [Reversible impairment of spermatogenesis induced by aromatic retinoid in guinea pigs (author's transl)].

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    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Tsambaos D,Hundeiker M,Mahrle G,Orfanos CE

    更新日期:1980-01-01 00:00:00

  • Detection of anti-envoplakin and anti-periplakin autoantibodies by ELISA in patients with paraneoplastic pemphigus.

    abstract::Paraneoplastic pemphigus patients (PNP) develop a group of autoantibodies, among which those against envoplakin and periplakin are almost always found. Epitope mapping has indicated that the linker subdomains of the proteins harbor the major antigenic sites recognized by PNP sera. In order to detect specific autoantib...

    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Huang Y,Li J,Zhu X

    更新日期:2009-10-01 00:00:00

  • GM-CSF downmodulates c-kit, Fc(epsilon)RI(alpha) and GM-CSF receptor expression as well as histamine and tryptase levels in cultured human mast cells.

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    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Welker P,Grabbe J,Zuberbier T,Grützkau A,Henz BM

    更新日期:2001-05-01 00:00:00

  • Active nuclear IKK correlates with metastatic risk in cutaneous squamous cell carcinoma.

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    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Toll A,Margalef P,Masferrer E,Ferrándiz-Pulido C,Gimeno J,Pujol RM,Bigas A,Espinosa L

    更新日期:2015-10-01 00:00:00

  • The expression of interleukin-8 receptor in untreated and treated psoriasis.

    abstract::The expression of IL-8 in psoriasis has been clearly shown with the use of immunocytochemical, RT-PCR and in situ hybridization methods. The presence of its ligand, the IL-8 receptor, has been demonstrated by the RT-PCR technique. We report here a study of the expression of both IL-8 type A and B receptors by immunohi...

    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Beljaards RC,Van Beek P,Nieboer C,Stoof TJ,Boorsma DM

    更新日期:1997-07-01 00:00:00

  • Adhesion of neutrophils to epidermal cells: prerequisites for and blocking by anti-CD11b antibodies.

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    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: von den Driesch P,Weller M,Wörl P

    更新日期:1997-11-01 00:00:00

  • Increased contact hypersensitivity response in mice by topical application of 1 alpha,25-dihydroxyvitamin D3 to elicitation site.

    abstract::Recent evidence indicates that the biologically active metabolite of vitamin D3, 1 alpha,25-dihydroxyvitamin D3 [1 alpha,25(OH)2D3], has an effect on the regulation of the immune response. We investigated whether topical treatment of mice with 1 alpha,25(OH)2D3 influences the contact hypersensitivity (CHS) response to...

    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Tani M,Murata Y,Harada S,Takashima T,Horikawa T

    更新日期:1989-01-01 00:00:00

  • Expression of lung resistance protein in epithelioid sarcoma in vitro and in vivo.

    abstract::The incidence of epithelioid sarcoma among patients with malignant soft tissue tumors is small, but the rates of recurrence and metastasis of this type of sarcoma are high. To date, effective chemotherapy for advanced epithelioid sarcoma has not been established and, furthermore, epithelioid sarcoma is known to exhibi...

    journal_title:Archives of dermatological research

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    authors: Kusakabe H,Iwasaki H,Sano K,Kiyokane K

    更新日期:2000-06-01 00:00:00

  • Does hypoxia play a role in infantile hemangioma?

    abstract::Infantile hemangioma (IH), the most common tumor of infancy, is characterized by rapid growth during infancy, followed by spontaneous involution over 5-10 years. Certain clinical observations have led to the suggestion that IH is triggered and maintained by hypoxia. We review the literature on the possible role of hyp...

    journal_title:Archives of dermatological research

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    authors: de Jong S,Itinteang T,Withers AH,Davis PF,Tan ST

    更新日期:2016-05-01 00:00:00

  • Skin levels of vasoactive intestinal polypeptide in atopic dermatitis.

    abstract::Atopic dermatitis (AD) can be exacerbated by various factors, including emotional stress, scratching and sweating. The aim of the present study was to evaluate the hypothesis that the inflammatory reaction in AD is also neurogenic. For this purpose, the levels of vasoactive intestinal polypeptide were measured radioim...

    journal_title:Archives of dermatological research

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    authors: Pincelli C,Fantini F,Romualdi P,Lesa G,Giannetti A

    更新日期:1991-01-01 00:00:00

  • Palmitate induces COX-2 expression via the sphingolipid pathway-mediated activation of NF-κB, p38, and ERK in human dermal fibroblasts.

    abstract::It has been suggested that free fatty acids (FFA) such as palmitate, which are secreted from enlarged adipocytes in the subcutaneous fat of obese subjects, serve as a link between obesity and altered skin functions. Cyclooxygenease-2 (COX-2) and prostanoids participate in the induction of impaired dermal function. In ...

    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Oh E,Yun M,Kim SK,Seo G,Bae JS,Joo K,Chae GT,Lee SB

    更新日期:2014-05-01 00:00:00

  • Hydroperoxides in oxidized d-limonene identified as potent contact allergens.

    abstract::Hydroperoxides of d-limonene were shown to be potent contact allergens when studied in guinea-pigs. Limonene-2-hydroperoxide (2-hydroperoxy-p-mentha-6,8-diene, a mixture of trans and cis isomers) was synthesized for the first time. The ratio between the trans and cis forms was 3:1. These two hydroperoxides were identi...

    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Karlberg AT,Shao LP,Nilsson U,Gäfvert E,Nilsson JL

    更新日期:1994-01-01 00:00:00

  • The effect of aromatic retinoid Ro 10-9359 (etretinate) on fingernail growth.

    abstract::Fingernail growth was studied in 28 patients who were receiving systemic treatment with the aromatic retinoid, Ro 10-9359 known as Tigason (etretinate; AR). A group of 15 patients with psoriasis was compared with a group of 13 patients with other dermatoses which are known to have a good response to AR therapy. In the...

    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Galosi A,Plewig G,Braun-Falco O

    更新日期:1985-01-01 00:00:00

  • Detection of fumarate-glutathione adducts in the portal vein blood of rats: evidence for rapid dimethylfumarate metabolism.

    abstract::Dimethylfumarate (DMF), the essential ingredient of the drug product Fumaderm®, is used to treat psoriasis with a recognized favorable long-term safety profile. Interestingly, the mode of action and the pharmacokinetics of DMF in psoriasis or multiple sclerosis are not fully explored. It is known that DMF as an α,β-un...

    journal_title:Archives of dermatological research

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    authors: Dibbert S,Clement B,Skak-Nielsen T,Mrowietz U,Rostami-Yazdi M

    更新日期:2013-07-01 00:00:00

  • Reversal of epidermal hyperproliferation in essential fatty acid deficient guinea pigs is accompanied by rapid generation of inositol triphosphate.

    abstract::This study probes the extent of coupling between the reversal of epidermal hyperproliferation induced by essential fatty acid (EFA) deficiency in guinea pigs, the hydrolysis of epidermal phosphatidylinositol 4,5-bisphosphate (PtdIns4,5P2), and the rapid formation of inositol triphosphate (InsP3). Our data revealed tha...

    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Tang W,Ziboh VA

    更新日期:1988-01-01 00:00:00

  • Percutaneous penetration of hydrocortisone in humans following skin delipidization by 1:1:1 trichloroethane.

    abstract::The effect of skin delipidization in humans on the percutaneous penetration of hydrocortisone was investigated in a cross-over trial. The delipidizing agent was 1:1:1 trichloroethane, a common industrial organic solvent. The findings do not show any significant difference in the topical absorption of hydrocortisone wi...

    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Bucks DA,Maibach HI,Menczel E,Wester RC

    更新日期:1983-01-01 00:00:00

  • Effects of different doses of abobotulinumtoxinA for the treatment of anterior gingival smile.

    abstract::Botulinum toxin type A (BoNT-A) is an easy and fast treatment for gingival smile (GS). The objective of the study was to compare the reduction of gingival exposure using three different doses of abobotulinumtoxinA (ABO) in patients with different severities of anterior GS, and to assess treatment safety and patients' ...

    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Hexsel D,Dal'Forno T,Camozzato F,Valente I,Soirefmann M,Silva AF,Siega C

    更新日期:2020-07-28 00:00:00

  • Modulation of type-IV procollagen and laminin production in A431 human squamous epidermoid carcinoma cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) and epidermal growth factor (EGF).

    abstract::The effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) and epidermal growth factor (EGF) on type-IV-procollagen (basement-membrane collagen) and laminin synthesis, turnover, and secretion was studied in human A431 squamous epidermoid carcinoma cells. Type-IV procollagen and laminin were biochemically and immunologic...

    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Panneerselvam M,Sahai A,Salomon DS

    更新日期:1985-01-01 00:00:00

  • Prolongation of skin graft survival in mice by in vitro PUVA treatment and failure of induction of specific immunological memory by PUVA-treated grafts.

    abstract::Treatment of murine skin grafts in vitro with 8-methoxypsoralen and longwave ultraviolet radiation prolonged their subsequent survival on allogeneic recipients, but not in cases where the recipients had been presensitized by a former skin graft of the same donor strain. In contrast to normal skin, grafts pretreated wi...

    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Gruner S,Meffert H,Karasek E,Sönnichsen N

    更新日期:1984-01-01 00:00:00

  • The quantitative distribution of percutaneously applied caffeine in the human skin.

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    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Zesch A,Schaefer H,Stüttgen G

    更新日期:1979-11-01 00:00:00

  • Pentane-1,5-diol as a percutaneous absorption enhancer.

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    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Faergemann J,Wahlstrand B,Hedner T,Johnsson J,Neubert RH,Nyström L,Maibach H

    更新日期:2005-12-01 00:00:00

  • Effect of theophylline on the transport of tyrosine in cultured B-16 mouse melanoma cells.

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    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Saga K,Shimojo T

    更新日期:1984-01-01 00:00:00

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    journal_title:Archives of dermatological research

    pub_type: 杂志文章


    authors: Meguro S,Arai Y,Masukawa Y,Uie K,Tokimitsu I

    更新日期:2000-09-01 00:00:00