Abstract:
:Bullous pemphigoid (BP) is an autoimmune blistering skin disease associated with autoantibodies against two hemidesmosomal proteins, BP180 (type XVII collagen) and BP230. As the pathogenic relevance of antibodies against the immunodominant NC16A domain of BP180 has been clearly demonstrated, specific removal of these antibodies should be a rational therapeutic approach. Here, we evaluated three recombinant forms of bacterially produced BP180 NC16A, a monomer, trimer, and tetramer, together with different matrices for their efficacy to specifically adsorb autoantibodies from BP plasma samples. An adsorber consisting of NC16A-trimer coupled to NHS-activated Sepharose 4 Fast Flow revealed satisfying adsorption rates and a high specificity. The NC16A-trimer adsorber was regenerable and autoclavable. It has the potential to be used for specific immunoadsorption to treat severe and refractory BP and other pemphigoid diseases associated with BP180 NC16A reactivity.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Mersmann M,Dworschak J,Ebermann K,Komorowski L,Schlumberger W,Stöcker W,Zillikens D,Probst C,Schmidt Edoi
10.1007/s00403-015-1606-7subject
Has Abstractpub_date
2016-01-01 00:00:00pages
31-8issue
1eissn
0340-3696issn
1432-069Xpii
10.1007/s00403-015-1606-7journal_volume
308pub_type
杂志文章abstract::Epidermolytic hyperkeratosis (EH) is a genetic disorder of keratins associated with epidermal differentiation. Affected individuals carry gene mutations for conserved sequences of keratins K1 or K10. The structural alterations of tonofilaments in EH seem to be a direct consequence of the keratin gene mutations. EH epi...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF01105793
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abstract::Fast antigen is a cell surface protein that mediates apoptosis. Using immunohistological, flow cytometry and electron microscopic analyses, we investigated the expression of Fas antigen on various skin tissues, and on cultured SV40-transformed human epidermal keratinocyte cell line KJD and human skin squamous cell car...
journal_title:Archives of dermatological research
pub_type: 杂志文章
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abstract::Because of its easy accessibility, the skin is a very attractive target for gene therapy purposes. To study potential clinical applications in a preclinical setting, appropriate animal models are needed. Pig skin is very similar to human skin, and a variety of human diseases that are potentially amenable to gene thera...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-006-0653-5
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abstract::Kaposi's sarcoma (KS) is a rare cutaneous tumor caused by human herpes virus-8 (HHV-8) infection that preferentially develops in case of severe immunosuppression, such as in HIV/AIDS disease. Haptoglobin (Hp), a polymorphic multifunctional plasma protein, exerts several immunomodulatory effects and is characterized by...
journal_title:Archives of dermatological research
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doi:10.1007/s00403-011-1161-9
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journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
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journal_title:Archives of dermatological research
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doi:10.1007/s00403-014-1485-3
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journal_title:Archives of dermatological research
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doi:10.1007/BF00509237
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journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-012-1250-4
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00404286
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journal_title:Archives of dermatological research
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doi:10.1007/s004030050168
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF02505038
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00412661
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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journal_title:Archives of dermatological research
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