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Antineoplastic effects of octreotide on human gallbladder cancer cells in vitro.

Abstract：

AIM:To investigate whether octreotide can inhibit the growth of human gallbladder cancer cells in vitro and to elucidate the antineoplastic mechanism of octreotide in gallbladder cancer. METHODS:A human gallbladder cancer cell line, GBC-SD, was cultured in vitro. The antiproliferative effects of octreotide were examined by means of an MTT assay and a colony forming ability assay. Morphological variation was investigated under scanning electron microscopy and transmission electron microscopy. Cell cycle analysis and apoptosis rate was evaluated by flow cytometry (FCM) after staining by propidium iodide. DNA fragmentation was assayed by agarose gel electrophoresis. Immunohistochemical staining was performed to evaluate the expressions of mutant-type p53 and bcl-2. RESULTS:The growth curve and colony forming ability assay showed significant inhibition of octreotide to the proliferation of GBC-SD cells in culture in a time- and dose-dependent manner. After exposure to octreotide, GBC-SD cells showed typically apoptotic characteristics, including morphological changes of chromatin condensation, vacuolar degeneration, nucleus fragmentation and apoptotic body formation. In FCM profile apoptotic cells showed increased sub-G(1) peaks in the octreotide group, significantly higher than the control group (P=0.013). There was also an augmentation in the cell proportion of G(0)/G(1) phase (P=0.015), while the proportion of S phase and G(2)/M phase remained unchanged (P=0.057 and P=0.280, respectively). DNA agarose gel electrophoresis displayed a ladder after exposure to 1 000 nmol/L octreotide. After being treated with octreotide, the expressions of both mutant-type p53 and bcl-2 decreased considering the percentage of positive cells (P<0.05). CONCLUSION:Octreotide has a negative action to the proliferation of GBC-SD cells, and the mechanism may be related to cytostatic and cytotoxic effects. The reduction of mutant-type p53 and bcl-2 expressions may be associated with the apoptosis induced by octreotide.

journal_name

World J Gastroenterol

journal_title

World journal of gastroenterology

authors

Wang JH,Xing QT,Yuan MB

doi

10.3748/wjg.v10.i7.1043

keywords:

subject

Has Abstract

pub_date

2004-04-01 00:00:00

pages

1043-6

issue

eissn

1007-9327

issn

2219-2840

journal_volume

pub_type

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Antineoplastic effects of octreotide on human gallbladder cancer cells in vitro.