Abstract:
AIM:To compare molecular profiles of proximal colon, distal colon and rectum in large adenomas, early and late carcinomas. To assess feasibility of testing directed at molecular markers from this study in routine clinical practice. METHODS:A prospective 3-year study has resulted in the acquisition of samples from 159 large adenomas and 138 carcinomas along with associated clinical parameters including localization, grade and histological type for adenomas and localization and stage for carcinomas. A complex molecular phenotyping has been performed using multiplex ligation-dependent probe amplification technique for the evaluation of CpG-island methylator phenotype (CIMP), PCR fragment analysis for detection of microsatellite instability and denaturing capillary electrophoresis for sensitive detection of somatic mutations in KRAS, BRAF, TP53 and APC genes. RESULTS:Molecular types according to previously introduced Jass classification have been evaluated for large adenomas and early and late carcinomas. An increase in CIMP+ type, eventually accompanied with KRAS mutations, was notable between large adenomas and early carcinomas. As expected, the longitudinal observations revealed a correlation of the CIMP+/BRAF+ type with proximal location. CONCLUSION:Prospective molecular classification of tissue specimens is feasible in routine endoscopy practice. Increased frequency of some molecular types corresponds to the developmental stages of colorectal tumors. As expected, a clear distinction is notable for tumors located in proximal colon supposedly arising from the serrated (methylation) pathway.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Minarikova P,Benesova L,Halkova T,Belsanova B,Suchanek S,Cyrany J,Tuckova I,Bures J,Zavoral M,Minarik Mdoi
10.3748/wjg.v22.i20.4936subject
Has Abstractpub_date
2016-05-28 00:00:00pages
4936-45issue
20eissn
1007-9327issn
2219-2840journal_volume
22pub_type
杂志文章abstract:AIM:To identify the differentially over-expressed genes associated with β-catenin accumulation in nuclei of hepatocellular carcinoma (HCC) cells. METHODS:Differentially expressed genes were identified in radiation-induced B6C3 F1 mouse HCC cells by mRNA differential display, Northern blot and RT-PCR, respectively. Tot...
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pub_type: 杂志文章
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journal_title:World journal of gastroenterology
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doi:10.3748/wjg.14.574
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更新日期:2016-04-07 00:00:00