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Different patterns of intestinal response to injury after arterial, venous or arteriovenous occlusion in rats.

Abstract：

AIM:To investigate the differences in injury patterns caused by arterial, venous or arteriovenous mesenteric occlusion. METHODS:Male Wistar rats were separated equally into four groups. Occlusion was performed by clamping the superior mesenteric artery (A), the mesenteric vein (V) or both (AV) for 30 min, followed by 60 min of reperfusion. A control group received sham surgery only. Intestinal sections were examined for histological damage and serum tumor necrosis factor-alpha (TNF-alpha), endothelin-1 (ET-1), P-selectin, antithrombin III (ATIII) and soluble intracellular adhesion molecule-1 (ICAM-1) concentrations were measured. RESULTS:All groups showed significant mucosal injury compared to controls. Furthermore, mucosal injury was significantly more severe in the V and AV groups compared to the A group (3.6 +/- 0.55, 3.4 +/- 0.55 and 2 +/- 0.71, respectively P = 0.01). ICAM-1 was similarly elevated in all groups, with no significant differences between the groups. P-selectin levels were significantly elevated in the V and AV groups but not the A group (1.4 +/- 0.5 ng/mL, 2.52 +/- 0.9 ng/mL and 0.02 +/- 0.01 ng/mL, respectively, P = 0.01) and ET-1 was significantly elevated in the A and V groups but not the AV group (0.32 +/- 0.04 pg/mL, 0.36 +/- 0.05 pg/mL and 0.29 +/- 0.03 pg/mL, respectively, P = 0.01) compared to sham controls. ATIII levels were markedly depleted in the V and AV groups, but not in the A group (29.1 +/- 5.2 pg/mL, 31.4 +/- 21.8 pg/mL and 55.8 +/- 35.6 pg/mL, respectively, P = 0.01), compared to controls. Serum TNF-alpha was significantly increased in all groups compared to sham controls (1.32 +/- 0.87 ng/mL, 1.79 +/- 0.20 ng/mL and 4.4 +/- 0.69 ng/mL, for groups A, V and AV, respectively, P = 0.01), with higher values in the AV group. CONCLUSION:Different patterns of response to ischemia/reperfusion are associated with venous, arterial or arteriovenous occlusion. Venous and arteriovenous occlusion was associated with the most severe alterations.

journal_name

World J Gastroenterol

journal_title

World journal of gastroenterology

authors

Guzmán-de la Garza FJ,Cámara-Lemarroy CR,Alarcón-Galván G,Cordero-Pérez P,Muñoz-Espinosa LE,Fernández-Garza NE

doi

10.3748/wjg.15.3901

subject

Has Abstract

pub_date

2009-08-21 00:00:00

pages

3901-7

issue

eissn

1007-9327

issn

2219-2840

journal_volume

pub_type

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Different patterns of intestinal response to injury after arterial, venous or arteriovenous occlusion in rats.