Complementary DNA-DNA hybridization in Drosophila.

Abstract:

:We have performed DNA-DNA hybridization experiments among several species of Drosophila using the evolutionarily conserved portion of the genome representing sequences coding for amino acids of proteins. This was done by using as tracer, radioactively labeled complementary DNA that was reverse transcribed from adult mRNA. We show that this procedure extends phylogenetically the distance over which the technique can be applied to fast-evolving groups such as Drosophila. The major phylogenetic conclusions are (1) the subgenus Sophophora is a monophyletic lineage; (2) within Sophophora the melanogaster subgroup is closer to the obscura group than either group is to the willistoni group; (3) the subgenus Drosophila is complex with most major lineages originating deep in the phylogeny; the subgenus may not be monophyletic; (4) as with most groups classically placed in Drosophila, the Hawaiian Drosophila originate early, supporting the notion that this lineage is older than the extant islands; and (5) the virilis/repleta lineage is monophyletic within Drosophila.

journal_name

J Mol Evol

authors

Caccone A,Gleason JM,Powell JR

doi

10.1007/BF00182390

keywords:

subject

Has Abstract

pub_date

1992-02-01 00:00:00

pages

130-40

issue

2

eissn

0022-2844

issn

1432-1432

journal_volume

34

pub_type

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