Abstract:
:We have performed DNA-DNA hybridization experiments among several species of Drosophila using the evolutionarily conserved portion of the genome representing sequences coding for amino acids of proteins. This was done by using as tracer, radioactively labeled complementary DNA that was reverse transcribed from adult mRNA. We show that this procedure extends phylogenetically the distance over which the technique can be applied to fast-evolving groups such as Drosophila. The major phylogenetic conclusions are (1) the subgenus Sophophora is a monophyletic lineage; (2) within Sophophora the melanogaster subgroup is closer to the obscura group than either group is to the willistoni group; (3) the subgenus Drosophila is complex with most major lineages originating deep in the phylogeny; the subgenus may not be monophyletic; (4) as with most groups classically placed in Drosophila, the Hawaiian Drosophila originate early, supporting the notion that this lineage is older than the extant islands; and (5) the virilis/repleta lineage is monophyletic within Drosophila.
journal_name
J Mol Evoljournal_title
Journal of molecular evolutionauthors
Caccone A,Gleason JM,Powell JRdoi
10.1007/BF00182390keywords:
subject
Has Abstractpub_date
1992-02-01 00:00:00pages
130-40issue
2eissn
0022-2844issn
1432-1432journal_volume
34pub_type
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更新日期:1994-09-01 00:00:00
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