Molecular characterization of the Rh-like locus and gene transcripts from the rhesus monkey (Macaca mulatta).

Abstract:

:The human Rh blood group locus consists of two structurally related genes (D and CcEe) in Rh-positive haplotypes but a single gene (CcEe) in Rh-negative haplotypes. The genome of rhesus monkeys (Macaca mulatta), while not expressing any of the human Rh D, C, c, E, or e specificities, carries a Rh-like locus strongly related to the human Rh locus. Southern blot analysis suggested the presence of only one Rh-like gene with an additional truncated fragment corresponding to the 5' region. RNA preparations from M. mulatta bone marrow cells contained Rh-like species of 1.7 kb. Two allelic Rh-like transcripts were amplified by PCR and sequenced. The predicted translation product of the first transcript was a 417-amino-acid protein closely similar to the human Rh counterpart. The predicted product of the second transcript consisted of a 361-amino-acid polypeptide truncated in the NH2 terminal region and differing from the former by a few substitutions. The macaque Rh-like protein sequences differed from those of human D and Cc/Ee polypeptides by 22-25%, whereas the degree of identity between the human proteins was 91.5%. Implications of these results in the analysis of the evolutionary pathway of the Rh locus are discussed.

journal_name

J Mol Evol

authors

Mouro I,Le Van Kim C,Cherif-Zahar B,Salvignol I,Blancher A,Cartron JP,Colin Y

doi

10.1007/BF00166163

subject

Has Abstract

pub_date

1994-02-01 00:00:00

pages

169-76

issue

2

eissn

0022-2844

issn

1432-1432

journal_volume

38

pub_type

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