Abstract：
:This study was designed to characterize further the nontranscribed intergenic spacers (NTSs) of the 5S rRNA genes of fish and evaluate this marker as a tool for comparative studies. Two members of the closely related North American Great Lakes cisco species complex (Coregonus artedi and C. zenithicus) were chosen for comparison. Fluorescence in situ hybridization found the ciscoes to have a single multicopy 5S locus located in a C band-positive region of the largest submetacentric chromosome. The entire NTS was amplified from the two species by polymerase chain reaction with oligonucleotide primers anchored in the conserved 5S coding region. Complete sequences were determined for 25 clones from four individuals representing two discrete NTS length variants. Sequence analysis found the length variants to result from presence of a 130-bp direct repeat. No two sequences from a single fish were identical. Examination of sequence from the coding region revealed two types of 5S genes in addition to pseudogenes. This suggests the presence of both somatic and germline (oocyte) forms of the 5S gene in the genome of Coregonus. The amount of variation present among NTS sequences indicates that accumulation of variation (mutation) is greater in this multicopy gene than is gene conversion (homogenization). The high level of sequence variation makes the 5S NTS an inappropriate DNA sequence for comparisons of closely related taxa.
journal_name
J Mol Evoljournal_title
Journal of molecular evolutionauthors
Sajdak SL,Reed KM,Phillips RBdoi
10.1007/pl00006348subject
Has Abstractpub_date
1998-06-01 00:00:00pages
680-8issue
6eissn
0022-2844issn
1432-1432journal_volume
46pub_type
杂志文章abstract：:The homeobox gene Hoxa-13 codes for a transcription factor involved in multiple functions, including body axis and hand/foot development in tetrapods. In this study we investigate whether the loss of one function (e.g., limb loss in snakes) left a molecular footprint in exon 1 of Hoxa-13 that could be associated with ...
journal_title：Journal of molecular evolution
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更新日期：2008-12-01 00:00:00
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journal_title：Journal of molecular evolution
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更新日期：2018-02-01 00:00:00
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journal_title：Journal of molecular evolution
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更新日期：2006-08-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
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更新日期：1998-09-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 社论
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更新日期：2018-04-01 00:00:00
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更新日期：2006-04-01 00:00:00
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更新日期：1988-01-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
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更新日期：1998-04-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF00182392
更新日期：1992-02-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-009-9283-9
更新日期：2009-10-01 00:00:00
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journal_title：Journal of molecular evolution
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更新日期：2019-07-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
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更新日期：2007-09-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s002390010074
更新日期：2000-08-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-012-9491-6
更新日期：2012-04-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-004-2606-y
更新日期：2004-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00239-016-9765-5
更新日期：2017-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1995-01-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
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更新日期：1981-01-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
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更新日期：2006-04-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/pl00006340
更新日期：1998-05-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
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更新日期：1995-08-01 00:00:00
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journal_title：Journal of molecular evolution
pub_type： 杂志文章
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更新日期：1993-03-01 00:00:00
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更新日期：2002-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：1994-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：1990-06-01 00:00:00
