Abstract：
:In this study, a new strategy for the selection of aptamers against small-molecule target was established using gold nanoparticles (AuNPs) as the separation matrix and Zinc(II)-Protoporphyrin IX (ZnPPIX) as the target molecule without the immobilization step due to the absorption of ssDNA on AuNPs. The progress of the selection process was monitored by the recovery rate and the fluorescence enhancement of N-methyl mesoporphyrin IX (NMM) after reacting with each selected pool. After 11 rounds of selection, a truncated aptamer ZnP1.2 with a low-micromolar dissociation constant was obtained, and it also showed good fluorescence enhancement for NMM and the enhanced peroxidase activity after binding with hemin, indicating this functional aptamer has potential to be a light-up fluorescent probe and a DNAzyme which could be used as an alternative to peroxidases for many colorimetric or chemiluminescent detections in biosensing events. The experimental results show that the simple and convenient AuNP-based SELEX is very conducive to the selection of aptamers for small-molecule targets.
journal_name
J Mol Evoljournal_title
Journal of molecular evolutionauthors
Li W,Luo Y,Gao T,Yang L,Wang J,Pei Rdoi
10.1007/s00239-019-09905-4subject
Has Abstractpub_date
2019-09-01 00:00:00pages
231-239issue
7-8eissn
0022-2844issn
1432-1432pii
10.1007/s00239-019-09905-4journal_volume
87pub_type
杂志文章abstract：:To clarify the phylogenetic position of Vestimentifera (tube worms), 346-bp fragments of the elongation factor-1 alpha (EF-1 alpha) gene (939-1286 according to the numbering of the human gene) of a vestimentiferan, Lamellibrachia sp., a sternaspid polychaete, Sternaspis scutata, an earthworm, Pheretima sp., and a gast...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF00170463
更新日期：1993-07-01 00:00:00
abstract：:In mammals, chicken-type (c-type) lysozymes are part of the innate immune system, killing bacteria by degrading peptidoglycan in their cell walls. Many of the studies on the evolution of c-type lysozymes have focused on its new digestive function, including the duplicated stomach lysozymes in ruminants. Similarly, in ...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-019-09910-7
更新日期：2019-12-01 00:00:00
abstract：:We characterized the organization of mitochondrial genes from a diverse range of hymenopterans. Of the 21 taxa characterized, 12 had distinct, derived organizations. Some rearrangements were consistent with the duplication-random loss mechanism, while others were not. Local inversions were relatively common, i.e., rea...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-002-2420-3
更新日期：2003-05-01 00:00:00
abstract：:In this study, the molecular population genetics of the orangutan's alpha-2 globin (HBA2) gene were investigated in order to test for the action of natural selection. Haplotypes from 28 orangutan chromosomes were collected from a 1.46-kilobase region of the alpha-2 globin locus. While many aspects of the data were con...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-004-0201-x
更新日期：2005-03-01 00:00:00
abstract：:We have studied the relative contribution of inversions, transpositions, deletions, and nucleotide substitutions to the evolution of Chlamydia trachomatis and Chlamydia pneumoniae. The minimal number of rearrangement events required for converting the gene order structure of one genome into that of the other was estim...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-001-0087-9
更新日期：2002-07-01 00:00:00
abstract：:Gene duplication creates a second copy of a gene either in tandem to the ancestral locus or dispersed to another chromosomal location. When the ancestral copy of a dispersed duplicate is lost from the genome, it creates the appearance that the gene was "relocated" from the ancestral locus to the derived location. Gene...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-018-9849-5
更新日期：2018-07-01 00:00:00
abstract：:Covarion processes allow changes in evolutionary rates at sites along the branches of a phylogenetic tree. Covarion-like evolution is increasingly recognized as an important mode of protein evolution. Several recent reports suggest that maximum likelihood estimation employing covarion models may support different opti...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-007-9062-4
更新日期：2008-01-01 00:00:00
abstract：:A problem with studying evolutionary dynamics of mitochondrial (mt) DNA is that classical population genetic techniques cannot identify selected substitutions because of genetic hitchhiking. We circumvented this problem by employing a candidate complex approach to study sequence variation in cytochrome c oxidase (COX)...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-008-9078-4
更新日期：2008-03-01 00:00:00
abstract：:Fimbrial adhesins allow bacteria to interact with and attach to their environment. The bacteria possibly benefit from these interactions, but all external structures including adhesins also allow bacteria to be identified by other organisms. Thus adhesion molecules might be under multiple forms of selection including ...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s002390010148
更新日期：2001-02-01 00:00:00
abstract：:The phylogenetic relationships among the major groups of amniote vertebrates remain a matter of controversy. Various alternatives for the position of the turtles have been proposed, branching off either before or after the mammals. To discover the phylogenetic position of turtles in relation to mammals and birds, we h...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02352288
更新日期：1996-05-01 00:00:00
abstract：:Gene duplication by retrotransposition duplicates only the coding and untranslated regions of a gene and, thus, biases retroduplicated genes toward having different expression patterns from their parental genes. As such, genes duplicated by retrotransposition are more likely to develop novel expression domains. To exp...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-008-9098-0
更新日期：2008-05-01 00:00:00
abstract：:The sequences of the adenylate kinase gene (adk) and the RecA gene (recA) were determined from the same isolates of Neisseria gonorrhoeae, N. meningitidis, N. lactamica, N. polysaccharea, N. cinerea, N. mucosa, N. pharyngis var. flava, N. flavescens, and N. animalis. The patterns of sequence divergence observed at adk...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF02202111
更新日期：1996-12-01 00:00:00
abstract：:Copia is a retrotransposon that appears to be distributed widely among the Drosophilidae subfamily. Evolutionary analyses of regulatory regions have indicated that the Copia retrotransposon evolved through both positive and purifying selection, and that horizontal transfer (HT) could also explain its patchy distributi...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-011-9435-6
更新日期：2011-03-01 00:00:00
abstract：:Phylogenetic hypotheses of muscle actin evolution are significantly different when a sea urchin is used as a representative echinoderm than when a sea star is used. While sea urchin muscle actins support an echinoderm-chordate sister relationship, sea star sequences suggest that echinoderm muscle actins are convergent...
journal_title：Journal of molecular evolution
pub_type： 评论,杂志文章
doi：10.1007/s002399910018
更新日期：2000-02-01 00:00:00
abstract：:The efficiency of obtaining the correct tree by the maximum likelihood method (Felsenstein 1981) for inferring trees from DNA sequence data was compared with trees obtained by distance methods. It was shown that the maximum likelihood method is superior to distance methods in the efficiency particularly when the evolu...
journal_title：Journal of molecular evolution
pub_type： 评论,杂志文章
doi：10.1007/BF02101285
更新日期：1991-05-01 00:00:00
abstract：:Oxygen-free aqueous solutions of NH4CN (0.1 M, pH9) were exposed to gamma rays from a 60Co source, the mixture of nonvolatile products was fractionated, and the fractions were analyzed. The procedures were chosen to make effective investigations of radiolytic products, and to minimize the contributions of chemical cha...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF01732951
更新日期：1980-07-01 00:00:00
abstract：:Circadian clock genes are remarkably conserved between eucoelomates. Although Drosophila has one copy of each major component, vertebrates have two or (in the case of the Period genes) three paralogs (Per1-3). We investigated the possibility that the vertebrate Per genes arose through two genome duplications during th...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-005-0185-1
更新日期：2006-06-01 00:00:00
abstract：:The human S100 gene family encodes the EF-hand superfamily of calcium-binding proteins, with at least 14 family members clustered relatively closely together on chromosome 1q21. We have analyzed the most recently available genomic sequence of the human S100 gene cluster for evidence of tandem gene duplications during ...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-002-2410-5
更新日期：2003-04-01 00:00:00
abstract：:Cot and chemical analysis show that the haploid genome size of Thermobia domestica is 3-4x10(9) nucleotide pairs. Of this DNA 33% is single copy sequences and 67% is repetitive sequences. The repetitive sequences are predominantly 300 nucleotides in length and are interspersed among the single copy sequences in a shor...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF01733135
更新日期：1980-08-01 00:00:00
abstract：:The Pten gene was initially identified in humans as a tumor suppressor. It has since been shown to play important roles in the control of cell size, cell motility, apoptosis, and organ size, and it has also been implicated in aging. Pten is highly conserved among organisms as diverse as nematodes, insects, and vertebr...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-005-0002-x
更新日期：2006-06-01 00:00:00
abstract：:Explicit evaluation of the accuracy and power of maximum likelihood and Bayesian methods for detecting site-specific positive Darwinian selection presents a challenge because selective consequences of single amino acid changes are generally unknown. We exploited extensive molecular and functional characterization of a...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-008-9172-7
更新日期：2008-11-01 00:00:00
abstract：:The major histocompatibility complex (MHC) consists of polymorphic frozen blocks (PFBs) that are linked to form megabase haplotypes. These blocks consist of polymorphic sequences and define regions where recombination appears to be inhibited. We have been able to show, using a highly polymorphic sequence centromeric o...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/pl00006194
更新日期：1997-07-01 00:00:00
abstract：:Life as we know it requires three basic types of polymers: polypeptide, polynucleotide, and polysaccharide. Here we evaluate both universal and idiosyncratic characteristics of these biopolymers. We incorporate this information into a model that explains much about their origins, selection, and early evolution. We obs...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-018-9876-2
更新日期：2018-12-01 00:00:00
abstract：:Evaporation of a solution of thymidine plus either the exo or the endo diastereomer of uridine cyclic 2',3'-O, O-phosphorothioate (U greater than p(S) in 1,2-diaminoethane hydrochloride buffer gave the 2',5' and 3',5' isomers of (P-thio) uridylylthymidine (Up(S)dT) in a ratio of 1:2 with a combined yield of about 20%....
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/BF01731369
更新日期：1979-11-01 00:00:00
abstract：:We have investigated the evolvability of an insoluble random polypeptide, RP3-34, to a soluble form through iterative mutation and selection with the aid of the green fluorescent protein (GFP) folding reporter. To assess the solubility of the polypeptides in the selected clones of each generation, the polypeptide gene...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-003-2542-2
更新日期：2004-02-01 00:00:00
abstract：:Statistical and biochemical studies of the genetic code have found evidence of nonrandom patterns in the distribution of codon assignments. It has, for example, been shown that the code minimizes the effects of point mutation or mistranslation: erroneous codons are either synonymous or code for an amino acid with chem...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/pl00006381
更新日期：1998-09-01 00:00:00
abstract：:Natural populations of the Midas cichlid species in several different crater lakes in Nicaragua exhibit a conspicuous color polymorphism. Most individuals are dark and the remaining have a gold coloration. The color morphs mate assortatively and sympatric population differentiation has been shown based on neutral mole...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s00239-010-9340-4
更新日期：2010-05-01 00:00:00
abstract：:The heterotrophic theory of the origin of life is the only proposal available with experimental support. This comes from the ease of prebiotic synthesis under strongly reducing conditions. The prebiotic synthesis of organic compounds by reduction of CO(2) to monomers used by the first organisms would also be considere...
journal_title：Journal of molecular evolution
pub_type： 杂志文章,评审
doi：
更新日期：1999-10-01 00:00:00
abstract：:The observed and expected frequencies of occurrence of microsatellites in the yeast Saccharomyces cerevisiae were investigated. In all cases, the observed frequencies exceeded the expected ones. In contrast to predictions by Messier et al. (1996), there is no critical number of repeats beyond which the observed freque...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/pl00006474
更新日期：1999-03-01 00:00:00
abstract：:We have previously shown that several multicopy gene families within the major histocompatibility complex (MHC) arose from a process of segmental duplication. It has also been observed that retroelements play a role in generating diversity within these duplicated segments. The objective of this study was to compare th...
journal_title：Journal of molecular evolution
pub_type： 杂志文章
doi：10.1007/s002399910042
更新日期：2000-04-01 00:00:00
