Abstract：
:Interleukin-6 (IL-6) is a multifunctional cytokine which has been suggested to function as an autocrine mitogen in psoriatic epidermis. We report here the results of several experiments designed to further examine this hypothesis. Blot hybridization was unable to detect 1.3 kb IL-6 transcripts in RNA extracted from normal or psoriatic epidermal (keratome) biopsies, suggesting that IL-6 expression is very low in normal and psoriatic epidermis. Therefore, qualitative and semiquantitative PCR/Southern blot analyses were performed on keratome-derived RNA, and revealed variable but significantly increased IL-6 mRNA levels in lesional psoriatic relative to normal tissue. To further examine the ability of normal human keratinocytes (NHK) to express IL-6, RNA was extracted from rapidly proliferating secondary NHK cultures. IL-6 transcripts were nearly undetectable by blotting in keratinocytes grown in low-calcium serum-free medium, but low levels could be induced by treatment with 1.8 mM CaCl2. IL-6 transcripts were strongly superinduced after cycloheximide treatment, suggesting that a labile protein regulates IL-6 mRNA levels in these cells. Finally, the mitogenic activity of IL-6 was examined in NHK under varying conditions of cell density and added growth factors. IL-6 did not stimulate high density keratinocyte growth in the presence or absence of other growth factors, but did stimulate clonal growth in epidermal growth factor (EGF)-deficient media at high concentrations (> or = 10 ng/ml). The proliferative effects of IL-6, but not of basic fibroblast growth factor, were abrogated by monoclonal antibodies directed against the EGF receptor.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Elder JT,Sartor CI,Boman DK,Benrazavi S,Fisher GJ,Pittelkow MRdoi
10.1007/BF00372034keywords:
subject
Has Abstractpub_date
1992-01-01 00:00:00pages
324-32issue
6eissn
0340-3696issn
1432-069Xjournal_volume
284pub_type
杂志文章abstract：:Psoriasis, a chronic inflammatory skin disease, is associated with oxidative stress of serum lipoproteins. In psoriatic children we evaluated the activity and levels of myeloperoxidase, the activity of paraoxonase-1 (PON1) and biochemical markers of lipid peroxidation, to investigate wether an unbalance between oxidan...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-019-01976-z
更新日期：2020-01-01 00:00:00
abstract：:Epidermolytic hyperkeratosis (EH) is a genetic disorder of keratins associated with epidermal differentiation. Affected individuals carry gene mutations for conserved sequences of keratins K1 or K10. The structural alterations of tonofilaments in EH seem to be a direct consequence of the keratin gene mutations. EH epi...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF01105793
更新日期：1995-01-01 00:00:00
abstract：:In the study described here we investigated the possibility of an association between the aggressiveness of melanoma and multidrug resistance phenotype by analyzing the expression and activity of P-glycoprotein (Pgp) in two genetically related transplantable hamster melanomas--a melanotic (Ma) and an amelanotic (Ab) f...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030000146
更新日期：2000-07-01 00:00:00
abstract：:Circulating and in situ mononuclear cell subsets were phenotypically characterized during both the acute and convalescent phase of herpes zoster infections in 14 patients. In peripheral blood a significant reduction in the absolute number of Leu 4+ T cells, Leu 2a+ suppressor/cytotoxic T cells, Leu 3a+ helper/inducer ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00412622
更新日期：1987-01-01 00:00:00
abstract：:Wound healing could be categorized as a chronic disorder which needs a prompt and effective medication. Nowadays, vascular problems and wound-healing progression studies relay on the role of metformin; however, the exact mechanisms for its beneficial effects are still not completely understood. The aim of the present ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-019-01982-1
更新日期：2020-03-01 00:00:00
abstract：:The phospholipids of the skin are difficult to quantify because they represent only a small fraction of the skin tissue. In this study, 31P nuclear magnetic resonance, which permits precise profiling of these phospholipids, was used to compare the phospholipids of upper eyelid epidermal and dermal lipid extracts (n = ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050308
更新日期：1998-06-01 00:00:00
abstract：:The dermal papilla plays pivotal roles in hair follicle cycle and dermal papilla cells (DPCs) with aggregative behavior have more obviously inductive ability. In the present study, the suppression subtractive hybridization method was employed to identify the differentially expressed genes in dermal papillae cells with...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-005-0583-7
更新日期：2005-09-01 00:00:00
abstract：:Paraneoplastic pemphigus patients (PNP) develop a group of autoantibodies, among which those against envoplakin and periplakin are almost always found. Epitope mapping has indicated that the linker subdomains of the proteins harbor the major antigenic sites recognized by PNP sera. In order to detect specific autoantib...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-008-0901-y
更新日期：2009-10-01 00:00:00
abstract：:Annexin A1 deregulation is often associated with cancer. Indeed we have shown that annexin A1 is overexpressed in melanoma and promotes metastases by formyl peptide receptor stimulation and MMP2 expression. Here, we demonstrated in different melanoma cell lines that annexin A1-MMP2 induction is mediated by MAPK and ST...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-014-1517-z
更新日期：2014-12-01 00:00:00
abstract：:Three new cromones have been studied that are supposed to be better absorbed and to have a wider spectrum of anti-allergic activity than disodium cromoglycate. Pretreatment with i.d. injection of 10 microgram FPL 52758 significantly reduced the weal and flare reaction induced by specific antigen in 11 patients with at...
journal_title：Archives of dermatological research
pub_type： 临床试验,杂志文章
doi：10.1007/BF00403843
更新日期：1980-01-01 00:00:00
abstract：:Dopamine causes reflex erythema, central blanching, and piloerection depending on the dose and the type of application wheal reaction. Intracutaneous application shows from 1.5 gamma/0.1 ml wheal formation, erythema, piloerection, and blanching combined with increased heat radiation from the skin surface (AGA thermovi...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00412863
更新日期：1979-08-01 00:00:00
abstract：:The incidence of epithelioid sarcoma among patients with malignant soft tissue tumors is small, but the rates of recurrence and metastasis of this type of sarcoma are high. To date, effective chemotherapy for advanced epithelioid sarcoma has not been established and, furthermore, epithelioid sarcoma is known to exhibi...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030000127
更新日期：2000-06-01 00:00:00
abstract：:In order to improve the characterization of human malignant melanoma cells and their variant gene expression in vitro, a search for specifically regulated genes was performed. Four melanoma cell lines (M5, MEWO, IGR39, SKMEL13) and newly cultured normal human melanocytes were included in a comparative hybridization (d...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00373422
更新日期：1995-01-01 00:00:00
abstract：:Bullous pemphigoid (BP) is an autoimmune blistering skin disease associated with autoantibodies against two hemidesmosomal proteins, BP180 (type XVII collagen) and BP230. As the pathogenic relevance of antibodies against the immunodominant NC16A domain of BP180 has been clearly demonstrated, specific removal of these ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-015-1606-7
更新日期：2016-01-01 00:00:00
abstract：:Permeation enhancers are defined as substances that are capable of promoting penetration of drugs into skin and transdermal therapeutic systems offers a more reliable mean of administering drug through the skin. Skin is a natural barrier so it is necessary to employ enhancement strategies to improve topical bioavailab...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/s00403-014-1445-y
更新日期：2014-07-01 00:00:00
abstract：:Recently, we described a novel fibroblast-restricted monoclonal antibody (mAb AS02) that recognizes a membrane-bound antigen. Characterization and isolation of the corresponding antigen showed that mAb AS02 recognized a protein on human fibroblasts that is highly homologous or identical to human Thy-1 antigen (CD90). ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050318
更新日期：1998-07-01 00:00:00
abstract：:Acrolein and 4-hydroxy-2-nonenal (HNE) are both byproducts of a lipid peroxidation reaction. Actinic elastosis in photodamaged skin of aged individuals is characterized by the accumulation of fragmented elastic fibers in the sun-exposed areas. To study whether a lipid peroxidation reaction is involved in the accumulat...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030100239
更新日期：2001-07-01 00:00:00
abstract：:Human epidermal keratinocytes were grown in culture and the uptake of hematoporphyrin derivatives (HPDs) used in photodynamic therapy was estimated. Keratinocytes loaded with HPDs were irradiated with laser light of 632 nm generated by a helium-neon laser and cell toxicity was determined by the trypan blue exclusion t...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00510086
更新日期：1989-01-01 00:00:00
abstract：:The mechanistic target of rapamycin (mTOR) is involved in the regulation of cellular growth, proliferation, lipid synthesis, and protein translation. The mTOR pathway involves two complexes: the mechanistic target of rapamycin complex 1 (mTORC1) and the mechanistic target of rapamycin complex 2 (mTORC2). Both mTOR com...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/s00403-014-1480-8
更新日期：2014-12-01 00:00:00
abstract：:As congenital nevocytic nevi have an increased risk of malignant degeneration, nevus-cell involvement in blood vessels and lymphatics is of particular interest. The present histological and ultrastructural studies revealed nevus-cell nests in the walls of venules in 1 out of 11 patients with medium-sized nevi, and in ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00412496
更新日期：1985-01-01 00:00:00
abstract：:Exposure in utero has been suggested to influence health later in life. The aim of this study was to investigate, if the use of prenatal food supplements was associated with atopic dermatitis in the offspring. Mothers who gave birth in the hospital G. B. Grassi were invited to participate in the study (n = 395). Infor...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-019-01911-2
更新日期：2019-07-01 00:00:00
abstract：:The expression of matrix metalloproteinase-2 was observed to be significantly upregulated in psoriasis. The aim of this study was to associate the DNA polymorphic variants in MMP-2 promoter gene with psoriasis and/or with psoriasis phenotypes related to psoriasis and comorbid heredity. In the total of 582 Czech Caucas...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-009-0947-5
更新日期：2009-07-01 00:00:00
abstract：:Eighteen patients with atopic dermatitis were randomly chosen for a clinical trial to compare the action of the H2 receptor antagonist cimetidine in combination with the H1 receptor antagonist chorpheniramine against the chlorpheniramine alone and against placebo. Each treatment period lasted for 4 weeks. Intensity of...
journal_title：Archives of dermatological research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/BF00412560
更新日期：1984-01-01 00:00:00
abstract：:The immigration of circulating T cells into specific tissues is directed by the interaction between adhesion molecules on lymphocyte subpopulations and their ligands on vascular endothelium. Of these, endothelial leucocyte adhesion molecule (ELAM-1), weakly expressed in normal human skin (NHS), seems to be the counter...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00372006
更新日期：1993-01-01 00:00:00
abstract：:Hidradenitis suppurativa (HS) is a chronic inflammatory disease associated with insulin resistance (IR), metabolic syndrome and increased cardiovascular risk. Adipokines are biologically active, pleotropic molecules which have been involved in the development of IR and in the pathogenesis of several chronic inflammato...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-019-02018-4
更新日期：2020-10-01 00:00:00
abstract：:An aromatic retinoid (Ro 10-9359) in 13-cis retinoic acid (Ro 4-3780) were fed by gastric tube to 170 male adult Syrian hamsters daily for 30 days. A low dose of 1 or 2 mg/kg and a high dose of 20 mg/kg body weight were selected. The effects of both retinoids on the sebaceous glands were assessed planimetrically (15 g...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00404284
更新日期：1980-01-01 00:00:00
abstract：:In human skin, epidermal differentiation occurs in two ways: normal differentiation, characterized by keratin 10 expression, and alternative differentiation. Alternative differentiation can be hyperproliferation-associated differentiation (expression of keratin 16) or the reinduction of an embryonic type of differenti...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/pl00007455
更新日期：2000-01-01 00:00:00
abstract：:Primary cultures of guinea pig ear keratinocytes were treated with vitamin A acid at plating, or at 14 h or 14 days after plating. The intracellular content of the cyclic nucleotides cAMP and cGMP was determined by radioimmunoassay at intervals during a period of 50 h after treatment. When added at plating, vitamin A ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00416918
更新日期：1980-01-01 00:00:00
abstract：:The aim of this study was to characterize the swelling behaviour of the stratum corneum. Stratum corneum pieces isolated from the breast region of 20 different females were incubated in distilled water at two different temperatures (20 degrees C and 45 degrees C) for 90 min and 24 h, respectively. Half of the stratum ...
journal_title：Archives of dermatological research
pub_type： 临床试验,杂志文章
doi：10.1007/s004030050231
更新日期：1997-08-01 00:00:00
abstract：:Whereas the inflammatory infiltrates of malignant melanoma have been widely investigated, little is known about the infiltrates accompanying benign melanocytic naevi. Using monoclonal antibodies directed against HLA-DR antigens, the CD1 antigen, the transferrin receptor and functionally divergent macrophage subpopulat...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00372703
更新日期：1992-01-01 00:00:00
