Abstract：
:The importance of IL-18, although clearly established in solid tumors, has not been fully elucidated in human hematopoietic neoplasms. Here we examined the mRNA and protein for IL-18 in eight human hematopoietic cell lines representing different lineages and neoplasms including leukemia, lymphoma and others. Our results revealed that IL-18 mRNA was expressed in these cells and that the corresponding protein was found in the cytoplasm. Seven of eight cell lines were also found to express two subunits of the IL-18 receptor (IL-18R) at varied levels. Furthermore, 29 out of 51 leukemia patients tested were observed to express IL-18R with 18/29 (62%) co-expression of both receptor and ligand. By blocking the IL-18 loop using specific antisense oligodeoxynucleotide (ASON) for IL-18 mRNA or anti-human IL-18R monoclonal antibody (McAbR), we were not able to demonstrate a marked inhibition on the most leukemic cell lines growth. Moreover, the potential proliferation in vitro of primary AML cells co-expressing IL-18 and its receptor was not significantly enhanced by recombinant human IL-18, suggesting that IL-18 is not apparently implicated in the proliferation of the leukemia cells via an autocrine loop. Additionally, we also found the effective modulating effect of M-CSF, IFN-alpha and TNF-alpha on IL-18R expression, implying an important in vivo effect of cytokines on IL-18-induced reaction. Moreover, the modulation of IL-18R expression was possibly irrelevant to IFN-gamma secretion induced by these cytokines.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Zhang B,Ma XT,Zheng GG,Li G,Rao Q,Wu KFdoi
10.1016/s0145-2126(03)00005-5keywords:
subject
Has Abstractpub_date
2003-09-01 00:00:00pages
813-22issue
9eissn
0145-2126issn
1873-5835pii
S0145212603000055journal_volume
27pub_type
杂志文章abstract：:The treatment of acute myeloid leukemia (AML) did not evolve profoundly in the last decades. Some improvement has been made for acute lymphoblastic leukemia (ALL). Emerging new treatment modalities, such as immunotherapy, are now beginning to be available for acute leukemia, mostly for patients suffering from ALL. Thi...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/j.leukres.2017.06.011
更新日期：2017-09-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:A consecutive series of acute myeloid leukemias (AML) patients was analyzed in conditions which reduce the inter-assay variations (the same flow cytometer, the same observers and the same panel of monoclonal antibodies) in order to investigate the prognostic information provided by flow cytome...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2005.02.015
更新日期：2005-10-01 00:00:00
abstract：:A wide range of different agents are capable of inducing the onset of HL-60 differentiation along the myeloid lineage. The diversity of these agents has made the analysis of the molecular mechanisms involved in the regulation of the onset and progress of terminal differentiation in these cells difficult. We have adapt...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(89)90081-7
更新日期：1989-01-01 00:00:00
abstract：:miRNAs have been reported to be involved in the pathogenesis of many cancers. In this article, we investigated the role and the mechanisms of miR-15a/16 in the pathogenesis of multiple myeloma (MM). We found that miR-15a/16 was down-regulated in bone marrow-derived mononuclear cells (BM-MNCs) of newly diagnosed patien...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2016.08.013
更新日期：2016-10-01 00:00:00
abstract：:When exposed to the anthracycline doxorubicin, K562 cells undergo differentiation which is characterized by arrested cell division, an increased mean cell diameter, and the production of hemoglobin. The influence of expression of p28, a low-molecular weight stress protein, on the differentiation of K562 cells was exam...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(00)00095-3
更新日期：2001-02-01 00:00:00
abstract：:Acute lymphoblastic leukemia (ALL) is the most common childhood malignancy. Although several clinical characteristics can be associated with worse prognosis, more robust biological markers still remains uncovered. SMYD2, a member of SMYD protein family, regulates the activity of several proteins through methylation. I...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2014.01.013
更新日期：2014-04-01 00:00:00
abstract：:Chemosensitivity of purified AML blasts to daunomycin was examined under conditions of CSF stimulation and compared with clinical outcome of DNR combination chemotherapy. AML blasts from 16 patients were purified, incubated serum-free in the presence of optimal concentrations of a complete cocktail of IL-3, GM-CSF and...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(90)90166-7
更新日期：1990-01-01 00:00:00
abstract：:Leukemic blasts of two patients with acute leukemia exhibited similar characteristics. They were heterogeneous in size with a diameter of 14-30 microns in smears and unclassifiable by morphological, cytochemical, immunophenotypic and ultrastructural examinations. Cytogenetic examinations of both revealed a near-tetrap...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(94)90087-6
更新日期：1994-07-01 00:00:00
abstract：:The effect of prostaglandin E2 (PGE2) on the reduction of c-myc expression during the differentiation of the human leukemic cell line, HL-60, was examined. PGE2, a potent inducer of intracellular cyclic AMP (cAMP) in HL-60 cells, augmented monocyte-associated cell surface antigens induced by human gamma-interferon (IF...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(88)90090-2
更新日期：1988-01-01 00:00:00
abstract：:Heterogeneity for 5'nucleotidase has been demonstrated in chronic lymphocytic leukemia [12]. The enzyme is not detectable in the lymphocytes from the majority of patients with this disorder, but normal and even supranormal levels are found in some cases [17]. In the present studies, the properties of this ecto-nucleot...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(82)90026-1
更新日期：1982-01-01 00:00:00
abstract：:Preclinical data suggest that all-trans retinoic acid (ATRA) synergizing with granulocyte colony stimulating factor (G-CSF), can improve the effectiveness of chemotherapy in acute myeloid leukemia (AML). Fludarabine 15 mg/m(2) is the minimum dose able to optimize intensification with fludarabine-arabinosylcytosine reg...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/j.leukres.2008.12.014
更新日期：2009-08-01 00:00:00
abstract：:Serial samples of peripheral blood were obtained from 75 children with acute lymphoblastic leukemia in remission. An immunofluorescence assay was used to quantitate TdT-containing (terminal deoxynucleotidyl transferase-containing) cells in the mononuclear leukocyte fraction of these specimens. Nine relapses in 8 patie...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(87)90089-0
更新日期：1987-01-01 00:00:00
abstract：:Recently, we identified a novel chromosomal rearrangement in Hodgkin lymphoma (HL), t(4;8)(q27;q24), which targets homeobox gene ZHX2 at the recurrent breakpoint 8q24. This aberration deletes the far upstream region of ZHX2 and results in silenced transcription pinpointing loss of activatory elements. Here, we have lo...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2011.10.019
更新日期：2012-05-01 00:00:00
abstract：:Inosine 5'-monophosphate (IMP) dehydrogenase catalyzes the rate-limiting reaction of guanine nucleotide biosynthesis and has been implicated in the reaction of cell growth and differentiation. We investigated the ability of mycophenolate mofetil, a prodrug of mycophenolic acid, to induce differentiation in HL-60 and U...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(00)00045-x
更新日期：2000-09-01 00:00:00
abstract：:A retrospective analysis of the relationship between the initial classification according to either FAB or WHO criteria, the presence of risk factors and the type of therapy including stem cell transplantation (SCT) on the survival was performed in a group of 106 patients with primary myelodysplastic syndrome (MDS) of...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2003.10.016
更新日期：2004-06-01 00:00:00
abstract：:The direct effect of the feline leukaemia virus (FeLV) on erythroid colony formation in vitro was investigated. Bone marrow mononuclear cells (BMMC) from FeLV-naïve, specific-pathogen-free (SPF), adult cats were inoculated with FeLVs of characterized strains and biologically cloned subgroups and the subsequent develop...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(86)90237-7
更新日期：1986-01-01 00:00:00
abstract：:Total body irradiation (TBI) has traditionally been used in the conditioning regimen for allogenetic hematopoietic stem cell transplantation (alloHCT) from an unrelated donor (u-HCT). However, patients are increasingly receiving a fludarabine-based conditioning regimen without TBI, as it seemed less toxic than TBI. We...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2014.01.002
更新日期：2014-07-01 00:00:00
abstract：:Endonucleases capable of producing internucleosomal DNA cleavage are one of the key enzymes in apoptosis. We examined endonuclease activities contained in nuclei of CD34+ and erythroid cells in the bone marrow (BM) from 12 patients with the myelodysplastic syndromes. The levels of Mg(2+)-dependent and acidic endonucle...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(97)00041-6
更新日期：1997-08-01 00:00:00
abstract：:TCL-1 expression is variable in CLL, and no study has examined its association with treatment response. We measured TCL-1 protein in CLL cells from 51 patients who then received pentostatin, cyclophosphamide, and rituximab. TCL-1 expression did not correlate with any pre-treatment characteristics. Lower TCL-1 levels w...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2007.05.020
更新日期：2007-12-01 00:00:00
abstract：:A single somatic mutation, V617F, in the pseudokinase domain of the Jak2 is the primary cause of many chronic myeloproliferative diseases. As valine 617 of Jak2 is conserved as valine 678 of Tyk2, we examined the effect of a homologous mutation in Tyk2 (V678F Tyk2) on cell growth. V678F Tyk2 augmented the transcriptio...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2006.08.018
更新日期：2007-08-01 00:00:00
abstract：:The polyamine transport system (PTS), hyperactive in cancer cells, can constitute a gate to deliver F14512, a novel spermine epipodophyllotoxin conjugate recently selected for clinical development in AML phase I. We investigated in vitro the high antiproliferative effect of F14512 against 13 leukemia cell lines, and d...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.12.021
更新日期：2010-10-01 00:00:00
abstract：:We have studied the survival of clonogenic neoplastic cells of a murine pre-B-lymphoma (BCl-1) of the spleen in culture. We have found quantitative deficiencies such as reduced surface adherence of stromal cells and impaired CFU-F (colony forming units-fibroblast) and pre-CFU-F colony and layer formation in stromal cu...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(93)90088-3
更新日期：1993-07-01 00:00:00
abstract：:There is resurgent interest in glucocorticoids (GCs) in the treatment of poor prognosis chronic lymphocytic leukemia (CLL). Little is known however on how GCs induce apoptosis in CLL. Methylprednisolone (MP) induces apoptosis in ZAP-70 positive CLL more readily than in ZAP-70 negative CLL, which is in contrast to the ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.02.022
更新日期：2009-10-01 00:00:00
abstract：:Many evidences have shown that dietary intake of cruciferous vegetables could protect against the risk of various types of malignancies. Benzyl isothiocyanate (BITC), one of the compounds from cruciferous vegetables, had shown induced cell cycle arrest and apoptosis in cancer cells. However, there is no available info...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.01.030
更新日期：2009-11-01 00:00:00
abstract：:We retrospectively evaluated the prognostic significance of polypharmacy and inappropriate medication use among 150 patients >60 years of age receiving induction chemotherapy for acute myelogenous leukemia (AML). After adjustment for age and comorbidity, increased number of medications at diagnosis (≥ 4 versus ≤ 1) wa...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2014.06.018
更新日期：2014-10-01 00:00:00
abstract：:Screening for loss of heterozygosity (LOH) of the panel of 18 highly polymorphic microsatellite markers, especially from the region 11p15, was carried out on 154 samples from 26 patients with acute myeloid leukemia and eight with myelodysplastic syndromes (MDS). LOH was detected at the majority (72%) of the loci teste...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(00)00086-2
更新日期：2001-01-01 00:00:00
abstract：BACKGROUND:The Philadelphia chromosome-negative myeloproliferative neoplasms (MPNs) are associated with increases in janus kinase 2 (JAK2) signaling, often resulting from the JAK2 V617F mutation. LY2784544 (gandotinib) is a potent, selective, small-molecule inhibitor of JAK2 that has potential dose-dependent selectivit...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2018.06.014
更新日期：2018-08-01 00:00:00
abstract：:The etiology of LGL leukemia is not known; however, we recently detected HTLV-II in a patient with LGL leukemia. In this study, we found that sera from 6 of 28 patients with LGL leukemia were positive for HTLV-I/II using a whole virus ELISA; moreover, the ELISA-negative sera were near the positive cut-off value. There...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(94)90078-7
更新日期：1994-06-01 00:00:00
abstract：BACKGROUND:Little is known on racial differences in patients with diffuse large B-cell lymphoma (DLBCL). The aim of this retrospective study is to compare characteristics, prognostic factors and outcomes of Asian and Western patients with DLBCL treated with rituximab, cyclophosphamide, doxorubicin, vincristine and pred...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2013.01.002
更新日期：2013-04-01 00:00:00
abstract：:The efficiency of gene transfer into human leukemia cell lines by electroporation was investigated. For both transient expression (beta-galactosidase gene) and stable transformation (neomycin resistance gene), the transfer efficiency into leukemia cell lines using a square wave pulse was superior to that using an expo...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(91)90062-x
更新日期：1991-01-01 00:00:00
