Expression of IL-18 and its receptor in human leukemia cells.


:The importance of IL-18, although clearly established in solid tumors, has not been fully elucidated in human hematopoietic neoplasms. Here we examined the mRNA and protein for IL-18 in eight human hematopoietic cell lines representing different lineages and neoplasms including leukemia, lymphoma and others. Our results revealed that IL-18 mRNA was expressed in these cells and that the corresponding protein was found in the cytoplasm. Seven of eight cell lines were also found to express two subunits of the IL-18 receptor (IL-18R) at varied levels. Furthermore, 29 out of 51 leukemia patients tested were observed to express IL-18R with 18/29 (62%) co-expression of both receptor and ligand. By blocking the IL-18 loop using specific antisense oligodeoxynucleotide (ASON) for IL-18 mRNA or anti-human IL-18R monoclonal antibody (McAbR), we were not able to demonstrate a marked inhibition on the most leukemic cell lines growth. Moreover, the potential proliferation in vitro of primary AML cells co-expressing IL-18 and its receptor was not significantly enhanced by recombinant human IL-18, suggesting that IL-18 is not apparently implicated in the proliferation of the leukemia cells via an autocrine loop. Additionally, we also found the effective modulating effect of M-CSF, IFN-alpha and TNF-alpha on IL-18R expression, implying an important in vivo effect of cytokines on IL-18-induced reaction. Moreover, the modulation of IL-18R expression was possibly irrelevant to IFN-gamma secretion induced by these cytokines.


Leuk Res


Leukemia research


Zhang B,Ma XT,Zheng GG,Li G,Rao Q,Wu KF





Has Abstract


2003-09-01 00:00:00














  • PD-1/PD-L1 expression in extra-medullary lesions of multiple myeloma.

    abstract::Multiple myeloma patients may develop extraosseous involvement in the course of the disease making prognosis very poor and new drugs clearly needed. The PD-1/PD-L1 axis has emerged as a master immune checkpoint in antitumor responses and recent studies investigated the role of PD-L1 in multiple myeloma cells; no data ...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Crescenzi A,Annibali O,Bianchi A,Pagano A,Donati M,Grifoni A,Avvisati G

    更新日期:2016-10-01 00:00:00

  • Pyrene butanol--an efficient, selective and non-metabolized photosensitizing agent for human myeloid leukemia cells.

    abstract::Methods for ex vivo purging of neoplastic cells from harvested marrow are being developed to increase the efficacy of autologous transplantation. One approach is selective photosensitization, using sensitizing compounds and light radiation. Pyrene-containing fatty acids and lipids are potent photosensitizers, e.g. 12-...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Fibach E,Rabia SA,Gatt S

    更新日期:1992-01-01 00:00:00

  • Parameters involved in the recognition of fresh human leukemic blasts by tumor-specific cytolytic T cell clones: a model study.

    abstract::Although clinical experience and in vitro data provide evidence of an anti-leukemic activity of T cells, there are few examples of recognition of leukemic cells by tumor-specific T cells in vitro. Tumor antigens encoded by the MAGE genes are useful tools to study this recognition. We tested the sensitivity to recognit...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Chambost H,Collette Y,Dutartre H,Thuret I,Olive D

    更新日期:2000-10-01 00:00:00

  • Impaired cell-mediated immunity to cytomegalovirus (CMV) in leukemic children with prolonged CMV viruria.

    abstract::To determine the role of cell-mediated immunity (CMI) to cytomegalovirus (CMV) in leukemic children after CMV infection, CMI to CMV antigen was studied using CMV-specific lymphocyte blastogenic responses (LBR) and interferon (IFN) production. Four children, who continuously secreted CMV in urine more than 2 years afte...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Ihara T,Yasuda N,Isaji M,Torigoe S,Ito M,Kamiya H,Sakurai M

    更新日期:1994-07-01 00:00:00

  • Constitutive production of interleukin-8 (IL-8) by normal and malignant human B-cells and other cell types.

    abstract::The culture supernatants from 43 human cell lines obtained during log phase and from purified normal peripheral blood B-lymphocytes cultured at 10(6) cells ml-1 for 48 h in RPMI 1640-5% fetal calf serum were examined for interleukin-8 (IL-8) using Elisa kits. Constitutive IL-8 production was found for 14/15 B-cell lin...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Srivastava MD,Srivastava R,Srivastava BI

    更新日期:1993-12-01 00:00:00

  • ER stress in diffuse large B cell lymphoma: GRP94 is a possible biomarker in germinal center versus activated B-cell type.

    abstract::The process of B-cell development is characterized by the activation of the unfolded protein response. Under certain circumstances, the unfolded protein response can be manipulated in a cell death-inducing way. Therefore, tackling the unfolded protein response might be an attractive strategy in the treatment of diffus...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Boelens J,Jais JP,Vanhoecke B,Beck I,Van Melckebeke H,Philippé J,Bracke M,Jardin F,Brière J,Leroy K,Offner F,Lust S

    更新日期:2013-01-01 00:00:00

  • Partial restoration of all trans retinoic acid (ATRA) sensitivity by compactin in ATRA-resistant leukemic cells (ATRA-R HL-60).

    abstract::The resistance to all trans retinoic acid (ATRA) differentiating treatment is a consequence, in most of the cases, of either increased catabolism or down regulation of ATRA uptake. Recently, we have shown that ATRA efficiency to differentiate HL-60 cells was enhanced about 30 times after its incorporation into Low Den...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Gueddari N,Madoulet C,Lefebvre P,Berthou L,Chomienne C,Jardillier JC

    更新日期:2000-06-01 00:00:00

  • Bone marrow plasma macrophage inflammatory protein protein-1 alpha(MIP-1 alpha) and sclerostin in multiple myeloma: relationship with bone disease and clinical characteristics.

    abstract::The aim of the study was to investigate the expression of MIP-1 alpha and sclerostin in bone marrow of patients with multiple myeloma (MM), the possible association of the sclerostin and MIP-1 alpha with MBD and the clinical characteristics. 53 patients (29 M, 24 F), median age 64 years was studied. MIP-1 alpha, scler...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Wang XT,He YC,Zhou SY,Jiang JZ,Huang YM,Liang YZ,Lai YR

    更新日期:2014-05-01 00:00:00

  • STAT6 and the regulation of CD23 expression in B-chronic lymphocytic leukemia.

    abstract::High CD23 expression is a hallmark of B-CLL cells. It is lost during in vitro culture and can be reinduced by IL-4, albeit to a lower extent than in normal B cells. To elucidate the events controlling CD23 expression in B-CLL cells, the IL-4 mediated induction of STAT6 was investigated. Western-blot analysis demonstra...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Kneitz C,Goller M,Seggewiss R,Yaman A,Serfling E,Tony HP

    更新日期:2000-04-01 00:00:00

  • Cytogenetic correlation with disease status and treatment outcome in advanced stage leukemia post bone marrow transplantation: a Southwest Oncology Group study (SWOG-8612).

    abstract::A retrospective cytogenetic study was performed to determine whether non-random chromosome aberrations were related to the outcome of marrow transplantation for advanced stage acute leukemia (AL) and chronic myelogenous leukemia (CML). The patients were registered on SWOG-8612, a randomized comparison of busulphan and...

    journal_title:Leukemia research

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Slovak ML,Kopecky KJ,Wolman SR,Henslee-Downey JP,Appelbaum FR,Forman SJ,Blume KG

    更新日期:1995-06-01 00:00:00

  • Effects of interleukin 2 on engraftment following autologous bone marrow transplantation (ABMT) in dogs.

    abstract::Beagle dogs were treated with recombinant human interleukin 2 (IL-2) 6 x 10(6) International Units (IU)/day for 7 consecutive days following conditioning with sublethal (200 cGy) or lethal (400 cGy) doses of high-dose rate whole body irradiation (WBI) and reconstitution with 2 x 10(8)/kg autologous bone marrow cells, ...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Samuel S,Nagler A,Or R,Slavin S

    更新日期:1992-10-01 00:00:00

  • Mutation screening for JAK2V617F: when to order the test and how to interpret the results.

    abstract::With the application of adequately sensitive tests, it is now becoming evident that more than 90% of patients with conventionally-defined polycythemia vera (PV) carry the somatic JAK2V617F mutation in their granulocytes. However, the specific mutation is also found in other classic and atypical myeloproliferative diso...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Tefferi A,Pardanani A

    更新日期:2006-06-01 00:00:00

  • Monoclonal antibodies raised against K562 cells reacted with human haematopoietic pluripotent stem cells.

    abstract::We describe the properties of three monoclonal antibodies (McAbs) (21H73, 37G7 and 49C12) against K562 cell surface antigens correlated with differentiation induced by 12-O-tetradecanoyl phorbol-13-acetate (TPA). Each of the McAbs immunoprecipitated K562 cell surface antigen with molecular weight (MW) of approximately...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Kubota Y,Tanaka T,Irino S

    更新日期:1991-01-01 00:00:00

  • Deoxycytidine kinase, thymidine kinase and cytidine deaminase and the formation of Ara-CTP in leukemic cells in different phases of the cell cycle.

    abstract::In this study we investigated the Ara-CTP-forming capacity of leukemic cells in different phases of the cell cycle. Cells from two leukemic cell lines and leukemic bone marrow cells from patients and rats (BNML model) with acute myelocytic leukemia were separated according to cell cycle phase by means of an albumin de...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Richel DJ,Colly LP,Arentsen-Honders MW,Starrenburg CW,Willemze R

    更新日期:1990-01-01 00:00:00

  • Genotype of glutathione S-transferase and other genetic configurations in myelodysplasia.

    abstract::We examined polymorphisms of glutathione S-transferase (GST) genes in 159 Japanese patients with myelodysplasia and compared the incidence with that in 43 normal individuals to clarify their pathogenetic significance in myelodysplasia. In individuals with the GSTT1 null genotype, the odds ratios for disease risk were ...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Sasai Y,Horiike S,Misawa S,Kaneko H,Kobayashi M,Fujii H,Kashima K,Taniwaki M

    更新日期:1999-11-01 00:00:00

  • Therapy-related myelodysplastic syndrome with inv(16)(p13q22) and I type CBFbeta/MYH11 after autologous transplantation: undetectable fusion transcript in pretransplant progenitor cells.

    abstract::We describe here a unique case of therapy-related myelodysplastic syndrome (t-MDS) with inv(16)(p13q22) after autologous stem cell transplantation for lymphoma. The rare and smallest I type CBFbeta/MYH11 fusion transcript with a breakpoint at nucleotide 399 of CBFbeta and at nucleotide 2134 of MYH11 was detected in th...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Yamamoto K,Nishikawa S,Minagawa K,Yakushijin K,Okamura A,Matsui T

    更新日期:2006-03-01 00:00:00

  • Changes in the surface marker pattern related to maturation in adult acute myeloid leukaemia.

    abstract::Introduction of the maturation index (MI) as a measure for the degree of maturation improved the subtyping of acute myeloid leukemia (AML). A comparison is made here between the MI and the results of surface marker analysis with a panel of monoclonal antibodies (McAb) in the immunofluorescence technique. The McAb appl...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: van Rhenen DJ,van der Reijden HJ,van't Veer MB,Engelfriet CP,von dem Borne AE,Langenhuijsen MM

    更新日期:1983-01-01 00:00:00

  • Ricin fusion toxin targeted to the human granulocyte-macrophage colony stimulating factor receptor is selectively toxic to acute myeloid leukemia cells.

    abstract::Treatment failure of patients with acute myelogenous leukemia (AML) is frequently due to the development of multidrug resistance phenotype blasts. We have expressed a fusion protein consisting of human granulocyte-macrophage colony stimulating factor (GMCSF) fused to the N-terminus of a lectin-deficient ricin toxin B ...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Burbage C,Tagge EP,Harris B,Hall P,Fu T,Willingham MC,Frankel AE

    更新日期:1997-07-01 00:00:00

  • Establishment of a novel cell line with T-lineage phenotype (HPB-MLp-W) from a non-Hodgkin's lymphoma patient.

    abstract::We report the characterization of a novel human T-cell line, HPB-MLp-W, which was established from blastic cells of a lymph node specimen from a patient with non-Hodgkin's lymphoma. They demonstrated the T-cell association antigens, CD2 and CD4, but no CD3, CD8, CD1, CD5, CD7 nor T-cell antigen receptor on their cell ...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Morikawa S,Morikawa K,Hara J,Nagasaki M,Nakano A,Oseko F

    更新日期:1991-01-01 00:00:00

  • Dequalinium induces cytotoxicity in human leukemia NB4 cells by downregulation of Raf/MEK/ERK and PI3K/Akt signaling pathways and potentiation of specific inhibitors of these pathways.

    abstract::Delocalized lipophilic cation dequalinium (DQA) selectively accumulates in mitochondria and displays anticancer activity in different malignancies. Our previous studies indicate a DQA-induced cytotoxicity in human acute promyelocytic leukemia NB4 cells by early disturbance in mitochondrial function and oxidative stres...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: García-Pérez AI,Galeano E,Nieto E,Estañ MC,Sancho P

    更新日期:2014-07-01 00:00:00

  • Tumoricidal activity of an acute promyelocytic leukemia cell line (HL-60) is augmented by human interferon alpha.

    abstract::Normal human peripheral blood polymorphonuclear leukocytes (PMNs) and cells from a human acute promyelocytic leukemia line (HL-60) were tested for cytotoxic potential against two human glioma and one normal fibroblast line. Both the PMNs and HL-60 exhibited significant cytotoxicity against the tumor targets while spar...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Buessow SC,Gillespie GY,Mahaley MS

    更新日期:1984-01-01 00:00:00

  • Minimal residual disease in vitro of a pre-B-lymphoma.

    abstract::We have studied the survival of clonogenic neoplastic cells of a murine pre-B-lymphoma (BCl-1) of the spleen in culture. We have found quantitative deficiencies such as reduced surface adherence of stromal cells and impaired CFU-F (colony forming units-fibroblast) and pre-CFU-F colony and layer formation in stromal cu...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Prindull G,Ben-Ishay Z,Bergholz M,Prindull B

    更新日期:1993-07-01 00:00:00

  • Pycnogenol induces differentiation and apoptosis in human promyeloid leukemia HL-60 cells.

    abstract::Pycnogenol, rich of many phytochemicals of medical value, is a commercialized nutrient supplement extracted from the bark of European coastal pine. In this study, we investigated the anti-tumor effects of Pycnogenol on HL-60, U937 and K562 human leukemia cell lines. We found that Pycnogenol inhibited cell proliferatio...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Huang WW,Yang JS,Lin CF,Ho WJ,Lee MR

    更新日期:2005-06-01 00:00:00

  • Human interleukin 4 regulates the phenotype of lymphocytes generated during mixed lymphocyte culture and inhibits the IL-2-induced development of LAK function in normal and leukaemic cells.

    abstract::This study examined the immunoregulatory role of recombinant interleukin 4 (IL-4), also known as B-cell stimulating factor 1, on the generation of cytotoxic effector cells from normal and leukaemic human blood mononuclear cells. When tested on cells from normal individuals, the addition of IL-4 to mixed lymphocyte cul...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Jin BQ,Lopez AF,Gillis S,Juttner CA,Vadas MA,Burns GF

    更新日期:1989-01-01 00:00:00

  • Aetiologic factors in lymphoid malignancies: a case-control epidemiological study.

    abstract::A prospective case-control study of the aetiologic factors involved in the production of lymphoid malignancies has been conducted within a defined geographical area covering six health districts in the Yorkshire Region. Among the aspects investigated were past medical events, occupations and certain social factors. A ...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Bernard SM,Cartwright RA,Bird CC,Richards ID,Lauder I,Roberts BE

    更新日期:1984-01-01 00:00:00

  • Soluble Kit receptor blocks stem cell factor bioactivity in vitro.

    abstract::Stem cell factor (SCF) is a growth factor that promotes the survival, proliferation, and differentiation of hematopoietic cells. SCF and its receptor, Kit, are normally present in both cell surface and soluble forms. Both forms of Kit can bind SCF. However, the function of soluble Kit is unknown. In order to determine...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Dahlen DD,Lin NL,Liu YC,Broudy VC

    更新日期:2001-05-01 00:00:00

  • Steroid effects on ZAP-70 and SYK in relation to apoptosis in poor prognosis chronic lymphocytic leukemia.

    abstract::There is resurgent interest in glucocorticoids (GCs) in the treatment of poor prognosis chronic lymphocytic leukemia (CLL). Little is known however on how GCs induce apoptosis in CLL. Methylprednisolone (MP) induces apoptosis in ZAP-70 positive CLL more readily than in ZAP-70 negative CLL, which is in contrast to the ...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Boelens J,Lust S,Van Bockstaele F,Van Gele M,Janssens A,Derycke L,Vanhoecke B,Philippé J,Bracke M,Offner F

    更新日期:2009-10-01 00:00:00

  • Haemopoietic stem cell: a new concept.

    abstract::It is generally accepted that morphologically recognizable bone marrow (BM) cells are derived from progenitor cells committed to a specific line of haematopoietic differentiation [51]. The origin and morphological identity of such progenitor cells is not yet known and the question, whether there is a single pluripoten...

    journal_title:Leukemia research

    pub_type: 杂志文章,评审


    authors: Islam A

    更新日期:1985-01-01 00:00:00

  • Production of interleukin-3 from a T-cell neoplasm.

    abstract::We have established a new T-cell line (CL-8313) that produces interleukin-3 from the spleen cells of mice with a radiation-induced myeloproliferative disorder. IL-3 activity was detected at an extremely high level in the culture medium of the CL-8313 cell line in the absence of any exogenous stimulator. A large amount...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Yoshida K,Seki M,Maeda M,Fujita J,Tadokoro K

    更新日期:1988-01-01 00:00:00

  • Mutation analysis of the DNA-damage checkpoint gene CHK2 in myelodysplastic syndromes and acute myeloid leukemias.

    abstract::Checkpoint genes code for a family of proteins which sense DNA damage in eukaryotic cells. They play an important role in the control of the cell cycle. The human CHK2 is a homolog of the yeast G(2) checkpoint kinases known as CDS1 and RAD53. The CHK2 may be a tumor suppressor gene because it was found to be mutated i...

    journal_title:Leukemia research

    pub_type: 杂志文章


    authors: Hofmann WK,Miller CW,Tsukasaki K,Tavor S,Ikezoe T,Hoelzer D,Takeuchi S,Koeffler HP

    更新日期:2001-04-01 00:00:00