Abstract：
:Inosine 5'-monophosphate (IMP) dehydrogenase catalyzes the rate-limiting reaction of guanine nucleotide biosynthesis and has been implicated in the reaction of cell growth and differentiation. We investigated the ability of mycophenolate mofetil, a prodrug of mycophenolic acid, to induce differentiation in HL-60 and U937 leukemic cells as well as in fresh leukemia cells from patients with non-lymphocytic leukemia. Treatment with mycophenolate mofetil reduced the intracellular guanosine 5'-triphosphate (GTP) levels and induced morphologic and functional differentiation in HL-60 and U937 cells dose-dependently. HL-60 and U937 cells developed macrophage-like cytoplasm as well as the expression of CD11b and CD14 antigens and the ability to oxidize nitroblue tetrazorium (NBT). These changes became evident when the intracellular GTP levels decreased to approximately 20-30% of the untreated control level and were abrogated by the addition of guanosine. In the fresh leukemic cells, differentiation induction was shown in the cells derived from seven of 13 patients. The fresh leukemia cells responding to mycophenolate mofetil revealed significant higher positivity to CD11b, CD14, and NBT before treatment and significantly reduced intracellular GTP levels after treatment compared to the non-responding cells. These findings suggest that mycophenolate mofetil induces differentiation in HL-60 and U937 cells and some fresh leukemia cells with moderate tendency to maturation, by causing a decrease in the intracellular GTP levels. Mycophenolate mofetil could be a promising differentiation inducer in vivo.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Inai K,Tsutani H,Yamauchi T,Fukushima T,Iwasaki H,Imamura S,Wano Y,Nemoto Y,Naiki H,Ueda Tdoi
10.1016/s0145-2126(00)00045-xkeywords:
subject
Has Abstractpub_date
2000-09-01 00:00:00pages
761-8issue
9eissn
0145-2126issn
1873-5835pii
S0145-2126(00)00045-Xjournal_volume
24pub_type
杂志文章abstract：:In this case-control study, interview data on smoking habits were available for 179 de novo cases (116 with cytogenetic data) of acute myeloid leukemia (AML) or myelodysplastic syndromes (MDS). Smoking habits were compared with a pooled set of population controls and hospital controls (diagnosed with malignant melanom...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2008.10.009
更新日期：2009-06-01 00:00:00
abstract：:We investigated the expression of fos oncogene proteins in lymphoproliferative disorders, using a monoclonal antibody (FO-120) that was prepared against a synthetic oligopeptide of fos protein (amino acid sequence from 127 to 152). Although peripheral blood leukocytes were rarely positive for FO-120, they were transie...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(89)90010-6
更新日期：1989-01-01 00:00:00
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journal_title：Leukemia research
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doi：10.1016/j.leukres.2006.07.022
更新日期：2007-05-01 00:00:00
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journal_title：Leukemia research
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doi：10.1016/j.leukres.2009.05.009
更新日期：2010-02-01 00:00:00
abstract：:Bone marrow specimens from 185 patients with plasma cell disorders (PCD) were investigated by fluorescence in situ hybridization (FISH) in order to determine the temporal sequence of cytogenetic aberrations. In 25 cases combined FISH analysis has also been performed at single cell level. Clonal evolution was observed ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2011.02.010
更新日期：2011-08-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2014.01.009
更新日期：2014-07-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
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更新日期：2014-05-01 00:00:00
abstract：:In a prospective study of 27 patients with acute myeloid leukaemia (AML) quality of life was studied using three different instruments, Life Ingredient Profile, Karnofsky Performance Scale and Vitagram. The course of the disease was followed during the induction period, remission, relapse and in the terminal phase. We...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(94)90028-0
更新日期：1994-04-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2010.10.008
更新日期：2011-08-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(94)90045-0
更新日期：1994-08-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
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更新日期：2003-06-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
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更新日期：2010-04-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(87)90117-2
更新日期：1987-01-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(91)90180-2
更新日期：1991-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0145-2126(85)90087-6
更新日期：1985-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.leukres.2004.12.001
更新日期：2005-07-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2008.02.011
更新日期：2008-09-01 00:00:00
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journal_title：Leukemia research
pub_type： 评论,社论
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更新日期：2009-06-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
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更新日期：1998-06-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
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更新日期：1988-01-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
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更新日期：1991-01-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章,meta分析,评审
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更新日期：2014-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：2003-05-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
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更新日期：2007-07-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
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更新日期：2004-02-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
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更新日期：2011-01-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
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更新日期：2019-11-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2012.08.017
更新日期：2013-01-01 00:00:00
abstract：:Smoking is associated with both acute myeloid leukemia (AML) and lung cancer. We therefore searched our database for concomitant presentation of AML and lung cancer. Among 775 AML cases and 5225 lung cancer cases presenting to Roswell Park Cancer Institute between the years January 1992 and May 2008 we found 12 (1.5% ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2008.12.016
更新日期：2009-09-01 00:00:00
