Abstract：
:A wide range of different agents are capable of inducing the onset of HL-60 differentiation along the myeloid lineage. The diversity of these agents has made the analysis of the molecular mechanisms involved in the regulation of the onset and progress of terminal differentiation in these cells difficult. We have adapted the standard soft-agar cloning procedure to enable the single-step selection of clonal populations of spontaneously arising differentiation resistant HL-60 variants. This simple procedure which obviates the need for mutagenesis, long-term exposure to inducing agents or complex manipulations, optimises the chance of obtaining variants with a single lesion blocking the onset of differentiation. The analysis of two variants, HL-R5 and HL-D4, selected by this procedure for resistance to retinoic acid and DMSO, respectively, suggests the existence of different pathways used by the two agents which converge before the onset of terminal differentiation.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Darling DC,Linskens MH,Farzaneh Fdoi
10.1016/0145-2126(89)90081-7subject
Has Abstractpub_date
1989-01-01 00:00:00pages
407-15issue
5eissn
0145-2126issn
1873-5835journal_volume
13pub_type
杂志文章abstract：:Experimental data are in keeping with the finding that megakaryocytes isolated from normal human bone marrow may promote fibroblast growth. This effect can be significantly enhanced by interleukin (IL)-3. In this context it has been demonstrated that IL-3 induces the release of platelet-derived growth factor (PDGF) an...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(99)00091-0
更新日期：1999-08-01 00:00:00
abstract：:This study aimed at optimizing the conditions for flow cytometric detection of myeloperoxidase (MPO), cytoplasmic CD3 (cCD3), and cytoplasmic CD22 (cCD22), which seem to be more reliable lineage-associated markers in acute leukemia than surface antigens. Fixation methods employing saponin as detergent resulted in accu...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(93)90102-q
更新日期：1993-05-01 00:00:00
abstract：:Clofarabine (CLO), a purine nucleoside analog with promising efficacy in acute myeloid leukemia (AML), inhibits the ribonucleotidereductase, p53R2. We have shown that p53R2 mRNA is up-regulated by decitabine (DEC), another drug with promising activity in AML. We developed a pharmacodynamic model to characterize the in...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2012.07.015
更新日期：2012-11-01 00:00:00
abstract：:Although clinical experience and in vitro data provide evidence of an anti-leukemic activity of T cells, there are few examples of recognition of leukemic cells by tumor-specific T cells in vitro. Tumor antigens encoded by the MAGE genes are useful tools to study this recognition. We tested the sensitivity to recognit...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(00)00053-9
更新日期：2000-10-01 00:00:00
abstract：:This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.01.006
更新日期：2009-02-20 00:00:00
abstract：:Dasatinib was approved for the treatment of chronic phase (CP) chronic myeloid leukemia (CML) patients in first line therapy based on the demonstration of efficacy and safety reported in patients enrolled in clinical trials. We describe a multicentric Italian "real-life" experience of dasatinib used as frontline treat...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2015.11.008
更新日期：2016-01-01 00:00:00
abstract：:MDS is primarily a disease of the elderly. Cases who give a history of exposure to X-rays, cytotoxic drugs or leukaemogenic chemicals may be younger. Many cases of MDS present because of an incidental blood count. The most prominent clinical features are those of anaemia, neutropenia, thrombocytopenia. Because haemopo...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/0145-2126(92)90106-h
更新日期：1992-01-01 00:00:00
abstract：:We used a novel high-throughput drug screening assay, based on Luminex technology, to identify anti-myeloma agents capable of inhibiting cytokines and growth factors essential for multiple myeloma (MM) from a chemical library of 1120 compounds provided by MMRF. Tetracycline derivatives inhibited MM cell proliferation ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.12.002
更新日期：2010-07-01 00:00:00
abstract：:Dimethyl sulfoxide (DMSO) is a widely used prototypical chemical inducer of cell differentiation. In the present study, the effects of DMSO on susceptibility of human myeloid leukemia U937 cells towards ligation of distinct death receptors (DRs) were investigated. DMSO sensitized cells towards induction of apoptosis b...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2005.05.016
更新日期：2006-01-01 00:00:00
abstract：:Methods for ex vivo purging of neoplastic cells from harvested marrow are being developed to increase the efficacy of autologous transplantation. One approach is selective photosensitization, using sensitizing compounds and light radiation. Pyrene-containing fatty acids and lipids are potent photosensitizers, e.g. 12-...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(92)90170-c
更新日期：1992-01-01 00:00:00
abstract：:Feulgen-DNA cytophotometry and 3H-thymidine autoradiography were used to evaluate therapeutic cytapheresis effects on cell cycle distributions of peripheral blood leukemic cells in 15 acute leukemia, 5 CML and 8 CLL patients and of bone marrow erythroblasts in 18 PV patients. Both individual cytapheresis procedures an...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(87)90186-x
更新日期：1987-01-01 00:00:00
abstract：:Philadelphia-positive chronic myeloid leukemia (Ph+ CML) patients who are resistant to imatinib are commonly treated with second-generation tyrosine kinase inhibitors (TKIs). Limited data exist on the possible effects of these drugs on subsequent allogeneic hematopoietic stem cell transplantation (allo-HSCT).The outco...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2009.04.036
更新日期：2010-02-01 00:00:00
abstract：:A total of 854 patients with anti-HTLV-I antibody positive ATL, newly diagnosed from 1983 to 1987, were analyzed for prognostic factors. There were 466 males and 388 females with a mean age of 57.1. Of these 269 (31.5%) were alive with a median follow-up time of 14 months from diagnosis, while 585 (65.5%) were dead wi...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(91)90087-a
更新日期：1991-01-01 00:00:00
abstract：:We have utilized the MTT assay to measure the metabolic activity of cells from the bone marrow of 55 patients with acute myeloid leukaemia (AML), myelodysplastic syndrome (MDS) and non-clonal disease. Doubling dilutions of cells were exposed to MTT for 3-4 h. The mean optical density of the formazan produced by each c...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(95)00163-8
更新日期：1996-05-01 00:00:00
abstract：:Recent studies indicate that lipid metabolic changes affect the survival of multiple myeloma (MM) cells. Time-of-flight secondary ion mass spectrometry (TOF-SIMS), an imaging mass spectrometry technique, is used to visualize the subcellular distribution of biomolecules including lipids. We therefore applied this metho...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2015.02.011
更新日期：2015-06-01 00:00:00
abstract：:Previous studies have shown that thymosin (fraction 5) is able to induce surface differentiation markers on normal murine bone marrow T-cell precursors. Phorbol ester (TPA) promotes differentiation of human leukaemic lymphoblasts as assessed by changes in phenotypic surface markers and terminal deoxynucleotidyl transf...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(83)90072-3
更新日期：1983-01-01 00:00:00
abstract：:Despite advances in understanding the complexities of acute myeloid leukaemia (AML), the treatment of refractory or relapsed AML (rrAML) remains a daunting clinical challenge. Numerous clinical trials have failed to identify new treatments or combinations of existing therapies that substantially improve outcomes and s...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/j.leukres.2016.05.025
更新日期：2016-08-01 00:00:00
abstract：:Five patients from the Kyushu area in Japan with adult T-cell leukaemia (ATL) refractory to conventional chemotherapeutic agents were treated with 5 mg/m2 of the adenosine deaminase inhibitor, 2'-deoxycoformycin (DCF), intravenously (i.v.) for 3 consecutive days, followed by 5 mg/m2 i.v. weekly. Two patients showed a ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(86)90251-1
更新日期：1986-01-01 00:00:00
abstract：:We have recently established the MV4-11 acute myelogenous leukemia (AML) subline, designated as MV4-11 TP53 R248W, which possesses a missense mutation (CGG→TGG; R248W) in the TP53 gene, leading to inactivation of this transcription factor. DNA methyltransferase (DNMT) inhibitor 5-Aza-2'-deoxycytidine (5-AzadC) induced...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2011.04.004
更新日期：2011-07-01 00:00:00
abstract：:We have previously shown that forced expression of the transcription factor Scl in WEHI-3B D(+) cells prevents ATRA-induced cell differentiation. We now find that the overexpression of Rbtn2 also interferes with induction of differentiation by ATRA. Addition of LiCl to ATRA treatment restored the capacity of both Scl-...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2003.08.011
更新日期：2004-04-01 00:00:00
abstract：BACKGROUND:Current results regarding treatment outcomes in acute myeloid leukemia (AML) point to significant differences between low- and middle-income countries (LMIC) and high-income countries (HIC). Excluding well-known socioeconomic issues, genetic markers important for prognosis have not been properly incorporated...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2017.07.005
更新日期：2017-09-01 00:00:00
abstract：:Homoharringtonine combined aclarubicin and cytarabine (HAA) has been demonstrated to achieve a high remission rate and provide a survival advantage in acute myeloid leukemia (AML). To investigate whether HAA is an ideal induction regimen for t(8;21)AML, we retrospectively analyzed the data of 140 patients from the las...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2016.02.012
更新日期：2016-05-01 00:00:00
abstract：:The aim of the study was to investigate the expression of MIP-1 alpha and sclerostin in bone marrow of patients with multiple myeloma (MM), the possible association of the sclerostin and MIP-1 alpha with MBD and the clinical characteristics. 53 patients (29 M, 24 F), median age 64 years was studied. MIP-1 alpha, scler...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2014.02.010
更新日期：2014-05-01 00:00:00
abstract：:Expression of p14(ARF) and p16(INK4a) tumor suppressor genes was investigated in 109 patients with chronic myeloid leukemia (CML). The p14(ARF) and p16(INK4a) mRNA levels were significantly low in patients in chronic phase (CP) at presentation and high in patients treated with interferon-alpha (IFN-alpha), especially ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2006.02.002
更新日期：2006-10-01 00:00:00
abstract：:The tumor promoting agent TPA (phorbol ester; 1.6 X 10(-8)M) was used to induce the differentiation in vitro of B-chronic lymphocytic leukemia (B-CLL) cells from 14 untreated patients. The uninduced phenotype was SIg+, Mrbc+, RFT-1+, RFA-4-, FMC7-. After 72 h incubation with TPA, B-CLL cells became RFA-4+, FMC7+ and l...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(84)90005-5
更新日期：1984-01-01 00:00:00
abstract：:In 2008 the first evidence-based Canadian consensus guideline addressing the diagnosis, monitoring and management of transfusional iron overload in patients with myelodysplastic syndromes (MDS) was published. The Canadian Consortium on MDS, comprised of hematologists from across Canada with a clinical and academic int...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/j.leukres.2018.09.005
更新日期：2018-11-01 00:00:00
abstract：:The K562 leukemia cell line has been extensively used in studies of erythroid differentiation but it has been less well appreciated that treatment of K562 cells with the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) leads to loss of their erythroid properties and to acquisition of several megakaryoblastoi...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/0145-2126(90)90002-q
更新日期：1990-01-01 00:00:00
abstract：:Beagle dogs were treated with recombinant human interleukin 2 (IL-2) 6 x 10(6) International Units (IU)/day for 7 consecutive days following conditioning with sublethal (200 cGy) or lethal (400 cGy) doses of high-dose rate whole body irradiation (WBI) and reconstitution with 2 x 10(8)/kg autologous bone marrow cells, ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(92)90075-i
更新日期：1992-10-01 00:00:00
abstract：:Fractionation of mononuclear cells from human fetal liver provides a cell population at early stages of myeloid differentiation which, when cultured, generates neutrophils and macrophages for up to a month. These studies describe the further purification of an undifferentiated myeloid blast cell population by rosette ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(90)90114-o
更新日期：1990-01-01 00:00:00
abstract：:Hyperdiploid and non-hyperdiploid multiple myeloma represents distinct biological entities characterized by different patterns of genetic changes. We sought to determine whether ploidy category (non-hyperdiploid versus hyperdiploid) remains stable over time from diagnosis to progression. Of the 43 patients studied (39...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/j.leukres.2005.07.004
更新日期：2006-03-01 00:00:00
