Abstract:
OBJECTIVE:To study telomerase activity (TA) and its variation in bone marrow mononuclear cells from patients with myelodysplastic syndrome (MDS) at different stages in comparison with normal bone marrow cells and leukemic cells. METHODS:The TA was semi-quantitatively determined in mononuclear cells from 20 normal bone marrow samples, 21 patients with MDS at different stages and 32 cases of acute leukemia by using a polymerase chain reaction-enzyme linked immuno-sorben assay (PCR-ELISA) kit. RESULTS:The TA in normal bone marrow cells was in the range of 0 to 0.3 units (U) with a mean of 0.11 +/- 0.08 U. Among them, 3 samples were considered positive in accordance with the standard recommended by the kit's pamphlet. In bone marrow cells from patients with acute leukemia, the TA was ranging from 0 to 0.96 U with a mean value of 0.42 +/- 0.26 U. The positive rate was 78.1% which was significantly different from that in normal bone marrow (BM) (P < 0.01). In case of myelodysplastic syndrome, the average level of TA was 0.27 +/- 0.19 U (ranging from 0 to 0.97 U) with a positive rate of 66.7%. In comparison with normal BM cells, the difference was significant (P < 0.05). Particularly, the MDS high-risk subgroup exhibited a significantly higher activity of telomerase (P < 0.05). In comparison with INT-1 and INT-2 subgroups in MDS patients based on international prognostic scoring system (IPPS), the difference in TA was also significant (P < 0.05). The abnormality in cell karyotype was not correlated with TA. CONCLUSION:The normal bone marrow cells demonstrate TA at a marginal level while a remarkably increasing level may be seen in acute leukemia patients. The BM cells from MDS patients display a moderate TA among which the high risk MDS subgroup with a poor prognostic IPPS score exhibited markedly higher TA.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Fu C,Chen Zkeywords:
subject
Has Abstractpub_date
2002-10-01 00:00:00pages
1475-8issue
10eissn
0366-6999issn
2542-5641journal_volume
115pub_type
杂志文章abstract:BACKGROUND:Asthma is a heterogeneous disease for which a strong genetic basis has been firmly established. Until now no studies have been undertaken to systemically explore the network of asthma-related genes using an internally developed literature-based discovery approach. This study was to explore asthma-related gen...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2013-07-01 00:00:00
abstract:BACKGROUND:Artificial joints present certain problems such as osteal absorption and lysis induced by wear debris which leads to loosening of the prosthesis over a period of time. Here we propose a design of an artificial magnetic suspension joint that was prepared by integrating the medical theories of modern material ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-12-01 00:00:00
abstract:OBJECTIVE:To evaluate the efficacy and safety of misoprostol in term labour induction. DATA SOURCES:Data from published English and Chinese literatures about misoprostol in term labour induction were identified from Medline and CBMdisk (using the search terms "misoprostol" and "labour induction") before 2001; hand sea...
journal_title:Chinese medical journal
pub_type: 杂志文章,meta分析,评审
doi:
更新日期:2004-03-01 00:00:00
abstract:OBJECTIVE:To investigate the significance of telomerase activity in urine in the diagnosis and recurrence surveillance of urothelial carcinoma. METHODS:Telomerase activity in urine of 54 cases of urothelial carcinoma (urothelial carcinoma group) was estimated by polymerase chain reaction-enzyme-linked immunosorbent as...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
abstract:Background:Automated peritoneal dialysis (APD) can cater to individual needs, provide treatment while asleep, take into account the adequacy of dialysis, and improve the quality of life. Currently, independent research and development of APD machines made in China are more conducive to patients. A randomized, multicent...
journal_title:Chinese medical journal
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.4103/0366-6999.246079
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pub_type: 杂志文章
doi:10.4103/0366-6999.156784
更新日期:2015-05-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:10.1097/CM9.0000000000000537
更新日期:2019-12-05 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2008-07-05 00:00:00
abstract::Klinefelter’s syndrome (KS) is the most common sex chromosome disease in men. Classical features of the syndrome include a eunuchoidal body habitus, small testes and hypergonadotrophic hypogonadism. There has been an increased risk of diabetes mellitus and autoimmune disease for KS patients. This paper reports a case ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-03-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.213959
更新日期:2017-09-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-05-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract:BACKGROUND:Adenomyosis is a gynecological disorder with symptoms most presenting as dysmenorrhea and heavy menstrual bleeding. However, the presence of lower urinary tract symptoms (LUTS) among women with adenomyosis remains unclear. This study was designed to determine the prevalence of LUTS and factors related to the...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.208232
更新日期:2017-07-05 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:10.4103/0366-6999.170257
更新日期:2015-12-05 00:00:00
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pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 临床试验,杂志文章
doi:
更新日期:2013-03-01 00:00:00
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pub_type: 杂志文章
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章,评审
doi:
更新日期:1997-11-01 00:00:00
abstract:OBJECTIVES:To study the interrelation between nitric oxide (NO) and endothelin-1 (ET1) in experimental acute hypoxic rats, and to evaluate the mechanism of acute hypoxic pulmonary hypertension affected by NO and ET1 and its intervention. METHODS:Nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemi...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
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