Molecular cloning and characterization of NAG-7: a novel gene downregulated in human nasopharyngeal carcinoma.

Abstract:

OBJECTIVE:To identify novel tumor suppressor genes at chromosome 3p24-26 in human nasopharyngeal carcinoma (NPC). METHODS:Twenty epithelial-derived expressed sequence tags (EST) were selected from chromosome 3p24-26. RT-PCR and Northern blot were used to detect the expression of the ESTs in NPC cell line, HNE-1, and primary cultures of normal nasopharyngeal epithelial cells. One EST, which was substantially downregulated in the HNE-1 cell line, was detected in 19 NPC biopsy samples. cDNA library screening was used to get its full sequence and the sequence of this novel gene was analyzed. RESULTS:A novel gene located at chromosome 3p25.3 was obtained and named NAG-7. It was downregulated in 26.3% (5/19) of NPC biopsy samples. Its 1677 bp full length cDNA had a potential open reading frame predicting a 94 amino acid protein with a molecular weight of 11,023.87 Dalton. Analysis of the NAG-7 gene showed that it was a transmembrane protein containing a protein kinase C phosphorylation site and a myristyl site. It has no significant homology to any reported genes in the database of GenBank. CONCLUSION:NAG-7 is a novel gene downregulated in NPC, suggesting that it may be involved in the development of NPC.

journal_name

Chin Med J (Engl)

journal_title

Chinese medical journal

authors

Xie Y,Bin L,Yang J,Li Z,Yu Y,Zhang X,Cao L,Li G

keywords:

subject

Has Abstract

pub_date

2001-05-01 00:00:00

pages

530-4

issue

5

eissn

0366-6999

issn

2542-5641

journal_volume

114

pub_type

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