Abstract:
OBJECTIVE:To identify novel tumor suppressor genes at chromosome 3p24-26 in human nasopharyngeal carcinoma (NPC). METHODS:Twenty epithelial-derived expressed sequence tags (EST) were selected from chromosome 3p24-26. RT-PCR and Northern blot were used to detect the expression of the ESTs in NPC cell line, HNE-1, and primary cultures of normal nasopharyngeal epithelial cells. One EST, which was substantially downregulated in the HNE-1 cell line, was detected in 19 NPC biopsy samples. cDNA library screening was used to get its full sequence and the sequence of this novel gene was analyzed. RESULTS:A novel gene located at chromosome 3p25.3 was obtained and named NAG-7. It was downregulated in 26.3% (5/19) of NPC biopsy samples. Its 1677 bp full length cDNA had a potential open reading frame predicting a 94 amino acid protein with a molecular weight of 11,023.87 Dalton. Analysis of the NAG-7 gene showed that it was a transmembrane protein containing a protein kinase C phosphorylation site and a myristyl site. It has no significant homology to any reported genes in the database of GenBank. CONCLUSION:NAG-7 is a novel gene downregulated in NPC, suggesting that it may be involved in the development of NPC.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Xie Y,Bin L,Yang J,Li Z,Yu Y,Zhang X,Cao L,Li Gkeywords:
subject
Has Abstractpub_date
2001-05-01 00:00:00pages
530-4issue
5eissn
0366-6999issn
2542-5641journal_volume
114pub_type
杂志文章abstract::Plasma levels of tissue plasminogen activator (tPA) antigen, tPA activity and plasminogen activator inhibitor (PAI) activity were determined in 40 cases of acute myocardial infarction (AMI) at the onset and 2-4 weeks after the onset of the disease. 24 healthy subjects served as controls. At the onset of AMI, mean plas...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
abstract:BACKGROUND:Mutation or deletion in the phosphatase and tensin homologue deleted on chromosome ten (PTEN) gene has been identified as an important cause of endometrial carcinoma; stromal cell derived factor-1alpha (SDF-1alpha) exerts growth-promoting effects on endometrial cancer cells through activation of the PI-3 kin...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2006-03-05 00:00:00
abstract::Apolipoprotein (a) [Lp(a)] phenotypes of 69 myocardial infarction survivor and 56 stroke patients were reported and compared to those of 190 healthy Chinese. The results revealed that the distributions of apo(a) phenotype frequency in patients with cardio-cerebrovascular disease (CCVD) were different from those of con...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2008-06-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND:After establishing a murine model of aminoglycoside antibiotic (AmAn) induced ototoxicity, the sensitivity of AmAn induced ototoxicity in three murine strains and the effect of kanamycin on the expression of Na-K-2Cl cotransporter-1 (NKCC1) in stria vascularis were investigated. METHODS:C57BL/6J, CBA/CaJ, N...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2006-06-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.152664
更新日期:2015-03-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
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更新日期:2012-05-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章,meta分析
doi:
更新日期:2013-07-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract:BACKGROUND:Patients with temporal lobe epilepsy (TLE) originating from different seizure onset zones had distinct electrophysiological characteristics and surgical outcomes. In this study, we aimed to investigate the relationship between the origin and prognosis of TLE, and the stereoelectroencephalography (SEEG) featu...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000001256
更新日期:2020-12-02 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.239306
更新日期:2018-09-05 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2009-03-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.4103/0366-6999.237396
更新日期:2018-08-05 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000000688
更新日期:2020-03-20 00:00:00
abstract:BACKGROUND:So far, most of the surgical techniques for congenital blepharophimosis syndrome are two-stage procedures. In this study, we investigated a modified one-stage procedure to reduce the suffering of patients. METHODS:From 2003 to 2005, we adopted an one-stage technique combining blepharoptosis correction with ...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2007-08-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2013-11-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract:BACKGROUND:Optical coherence tomography (OCT) is a high resolution noncontact imaging modality which can quantitatively detect the optic disc and retinal structure. This study was designed to evaluate the diagnostic capability of parameters of the optic disc, retinal nerve fiber layer thickness, and ganglion cell compl...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2010-08-05 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2012-03-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2009-05-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章,多中心研究
doi:
更新日期:1995-04-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2003-01-01 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract:BACKGROUND:Spinal cord injury (SCI) is a worldwide medical concern. This study aimed to elucidate the mechanism underlying the protective effect of hyperbaric oxygen (HBO) against SCI-induced neurologic defects in rats via exploring the stromal cell-derived factor-1 (SDF-1)/CXC chemokine receptor 4 (CXCR4) axis and exp...
journal_title:Chinese medical journal
pub_type: 杂志文章
doi:10.1097/CM9.0000000000000115
更新日期:2019-03-20 00:00:00
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journal_title:Chinese medical journal
pub_type: 杂志文章
doi:
更新日期:2009-08-20 00:00:00