Abstract：
BACKGROUND:A practical problem impeding clinical translation is the limited bone formation seen in artificial bone grafts. Low-pressure/vacuum seeding and dynamic culturing in bioreactors have led to a greater penetration into the scaffolds, enhanced production of bone marrow cells, and improved tissue-engineered bone formation. The goal of this study was to promote more extensive bone formation in the composites of porous ceramics and bone marrow stromal cells (BMSCs). METHODS:BMSCs/β-tricalcium phosphate (β-TCP) composites were subcultured for 2 weeks and then subcutaneously implanted into syngeneic rats that were split into a low-intensity pulsed ultrasound (LIPUS) treatment group and a control group. These implants were harvested at 5, 10, 25, and 50 days after implantation. The samples were then biomechanically tested and analyzed for alkaline phosphate (ALP) activity and osteocalcin (OCN) content and were also observed by light microscopy. RESULTS:The levels of ALP activity and OCN content in the composites were significantly higher in the LIPUS group than in the control group. Histomorphometric analysis revealed a greater degree of soft tissue repair, increased blood flow, better angiogenesis, and more extensive bone formation in the LIPUS groups than in the controls. No significant difference in the compressive strength was found between the two groups. CONCLUSION:LIPUS treatment appears to enhance bone formation and angiogenesis in the BMSCs/β-TCP composites.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Wang J,Wang J,Yoshinori A,Paul F,Shen H,Chen J,Sotome S,Liu Z,Shinomiya Ksubject
Has Abstractpub_date
2014-01-01 00:00:00pages
669-74issue
4eissn
0366-6999issn
2542-5641journal_volume
127pub_type
杂志文章abstract：OBJECTIVE:To study the effect of pericardial devascularization combined with Latarjet's innervation on portal hypertension. METHODS:Forty-eight patients undergoing pericardial devascularization combined with Latarjet's innervation were compared with 57 patients with devascularization. Clinical results were evaluated b...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：OBJECTIVE:To evaluate the feasibility, safety and diagnostic accuracy of pharmacologic stress of (99m)Technetium-MIBI single-photon emission computed tomography (SPECT) with intravenous adenosine triphosphate (ATP) in patients with suspected coronary artery disease. METHODS:The study group included 263 patients who we...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:Psoralen (PSO) was found cytotoxic against in vitro cultured human mucoepidermoid carcinoma cells of MEC-1 cell line. Its IC50 value was 8.6 micrograms/ml, and relative antitumor activity (RAA) 15. PSO suppressed DNA synthesis, damaged microvilli and cell membrane, and induced degeneration of mitochondria. It inhibite...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1992-11-01 00:00:00
abstract：OBJECTIVE:To investigate whether PreS1 antigen and/or anti-PreS1 antibody in serum might have some significance different from routine hepatitis B virus (HBV) markers in hepatitis B virus infection. METHODS:Forty-six acute hepatitis B patients and 377 chronic persistent hepatitis patients were examined to assess the c...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
abstract：:To develop a more comprehensive index for predicting the prognous of liver cirrhosis. 300 consecutive patients with cirrhosis were studied in terms of survival from 1975 to 1986. Median follow-up period was 5.3 years. A multivariable survival analysis (Cox's regression model) using clinical biochemical data obtained a...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:Dextrocardia is a rare anomaly where the heart is located on the right side of the chest instead of the normal left side. Ablation of atrial fibrillation (AF) with such an inverted anatomy may be challenging for the manipulation of the catheters. Here we report a case of dextrocardia who underwent ablation for persist...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-05-01 00:00:00
abstract：BACKGROUND:The most common obstruction sites for obstructive sleep apnea hypopnea syndrome (OSAHS) are the oropharynx and the glossopharyx. The diagnosis of glossopharyngeal airway obstruction is difficult. The study aimed to assess the effect of upper airway reconstructive surgery for OSAHS based on polysomnography (P...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：OBJECTIVE:To focus on the asthmatic pathogenesis and clinical manifestations related to epithelial sodium channel (ENaC)/chlorine ion channel. DATA SOURCES:The data analyzed in this review were the English articles from 1980 to 2015 from journal databases, primarily PubMed and Google Scholar. The terms used in the lit...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：10.4103/0366-6999.162494
更新日期：2015-08-20 00:00:00
abstract：OBJECTIVE:The aim of this study was to summarize recent studies on nondopaminergic options for the treatment of motor symptoms in Parkinson's disease (PD). DATA SOURCES:Papers in English published in PubMed, Cochrane, and Ovid Nursing databases between January 1988 and November 2016 were searched using the following k...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：10.4103/0366-6999.211555
更新日期：2017-08-05 00:00:00
abstract：:Ninety-five patients with perforation, hemorrhage or stenosis due to duodenal ulcer were treated by extended parietal cell vagotomy. Postoperative follow-up ranged from 3.5 to 10 years (mean 6 years) in 88 patients (92%) with acute perforation (60), hemorrhage (8) and stenosis (20). There was no operative mortality. U...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of active immunotherapy with anti-idiotypic vaccine in patients with nasopharyngeal carcinoma (NPC). METHODS:Anti-idiotypic antibodies (2H4/5D3) bearing the internal image of the NPC antigen were used in active immunotherapy in NPC patients receiving radiotherapy. Antibodies and cyt...
journal_title：Chinese medical journal
pub_type： 临床试验,杂志文章
doi：
更新日期：2002-04-01 00:00:00
abstract：OBJECTIVE:To investigate the effects of gypenosides (Gs) on cellular immunocompetence in the gamma-ray-irradiated mice. MATERIALS AND METHODS:Tested mice of ICR strain were treated continuously with Gs for 10 days before or after 4 Gy gamma-irradiation. Body weight and splenic weight of mice were measured. The blastog...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1996-02-01 00:00:00
abstract：BACKGROUND:With the development of magnetic resonance (MR) technologies, whole-body 3D contrast-enhanced MR angiography (3D CE MRA) has become possible. The purpose of this study was to introduce and evaluate this technique in demonstration of various systemic arterial diseases. METHODS:Thirty-seven patients underwent...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2006-11-05 00:00:00
abstract：BACKGROUND:In county-level tuberculosis (TB) dispensaries in China, the accurate diagnosis of sputum smear-negative pulmonary tuberculosis (SNPT) needs to be improved by developing and validating clinical and radiographic predictors. METHODS:The study was conducted simultaneously in three counties per province in Chon...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：:100 women ranging in age from 26-34 years were followed up for 4-21 months after undergoing sterilization by tubal occlusion using intraluminal threads and silver clips. The number of children/woman ranged from 1-5 with 80% having 2. 31 women underwent sterilization postmenstrually, 11 midmenstrually, 28 premenstrua...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1983-08-01 00:00:00
abstract：BACKGROUND:Mitochondrial DNA 4977-bp deletion (DeltamtDNA(4977)) was reported in many human neoplasia. However, its biological significance remains to be evaluated and the molecular mechanism needs to be investigated. In this study, we analyzed the frequency of DeltamtDNA(4977) in gastric cancer (GC) cell lines and tis...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-02-20 00:00:00
abstract：BACKGROUND:Recent studies have suggested that cancer stem cells cause tumor recurrence based on their resistance to radiotherapy and chemotherapy. Although the highly invasive nature of glioblastoma cells is also implicated in the failure of current therapies, it is not clear whether cancer stem cells are involved in i...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-09-01 00:00:00
abstract：OBJECTIVE:To explore the management of cerebral ischemia caused by Takayasu's arteritis. METHODS:Ninety-three cases treated from June 1984 to September 1999 at the General Post & Telecom Hospital, the Sir Run Run Shaw Hospital, the First Affiliated Hospital of Zhejiang University, the Second Medical College of Beijing...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：BACKGROUND:Serum creatinine (Scr) measurement plays a key role in glomerular filtration rate estimation (eGFR), chronic kidney disease (CKD) diagnosis as well as CKD treatment. However, the test results of Scr from different laboratories vary significantly. In order to get comparable results, the European in vitro diag...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
abstract：OBJECTIVE:To report the surgical techniques and results of an 8-year follow-up study of 42 patients with a modified orthotopic ileal neobladder restoring normal anatomical relationship. METHODS:Total cystoprostatectomy was performed extraperitoneally. A 45 - 50 cm segment of the ileal loop was isolated, detubularized,...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
abstract：Objective:Mechanical ventilation (MV) has long been used as a life-sustaining approach for several decades. However, researchers realized that MV not only brings benefits to patients but also cause lung injury if used improperly, which is termed as ventilator-induced lung injury (VILI). This review aimed to discuss the...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：10.4103/0366-6999.226840
更新日期：2018-05-20 00:00:00
abstract：BACKGROUND:The Xpert MTB/RIF showed high sensitivity and specificity in previous studies carried out in different epidemiological and geographical settings and patient populations in high-burden tuberculosis (TB) countries. However, there were little data obtained by validation or demonstration study of the assay in Ch...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:Early diagnosis and accurate staging are important to improve the cure rate and prognosis for pancreatic cancer. This study was performed to develop an automatic and accurate imaging processing technique system, allowing this system to read computed tomography (CT) images correctly and make diagnosis of panc...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000000544
更新日期：2019-12-05 00:00:00
abstract：Background:Myoclonic epilepsy with ragged red fibers (MERRF) syndrome is characterized by myoclonus, generalized epilepsy, cerebellar ataxia, and ragged red fibers (RRFs) in the muscle. T-to-C transition at nucleotide position 14709 in the mitochondrial tRNA glutamic acid (tRNAGlu) gene has previously been associated w...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.235120
更新日期：2018-07-05 00:00:00
abstract：BACKGROUND:So far, most of the surgical techniques for congenital blepharophimosis syndrome are two-stage procedures. In this study, we investigated a modified one-stage procedure to reduce the suffering of patients. METHODS:From 2003 to 2005, we adopted an one-stage technique combining blepharoptosis correction with ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2007-08-20 00:00:00
abstract：:Endolymphatic sac papillary tumor (ELST) is an extremely rare and aggressive tumor characterized by hearing loss and temporal bone destruction. A case with clinical, imaging, pathologic and treatment data is reported and relevant literature is reviewed. A 25-year-old woman, with ELST underwent craniotomy for tumor sub...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：2011-11-01 00:00:00
abstract：BACKGROUND:Four single nucleotide polymorphisms (SNPs) in the modulator recognition factor 2/AT-rich interaction domain 5B (MRF2/ARID5B) gene located at chromosome 10q21.2 have been shown to be associated with both type 2 diabetes mellitus (T2DM) and coronary artery disease in a Japanese cohort. This study aimed to inv...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.204926
更新日期：2017-05-05 00:00:00
abstract：BACKGROUND:Rituximab is used extensively in combination with chemotherapy to cure non-Hodgkin's lymphoma (NHL), and not only accelerates short-term improvement, but also prolongs patient survival and decreases receptor relapse. The aim of this study was to evaluate the impact of Fcgamma IIIA (FcgammaRIIIA) gene polymor...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-01-20 00:00:00
abstract：BACKGROUND:Angiogenesis and lymphogenesis which were promoted by vascular endothelial growth factor (VEGF) and VEGF-C are important in the growth and metastasis of solid tumors. The high level of VEGF and VEGF-C were distributed in numerous types of cancers, but their distribution and expression in Wilms tumor, the mos...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-11-01 00:00:00
abstract：BACKGROUND:Green tea has been shown to improve cholesterol metabolism in animal studies, but the molecular mechanisms underlying this function have not been fully understood. Long non-coding RNAs (lncRNAs) have recently emerged as a major class of regulatory molecules involved in a broad range of biological processes a...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.147824
更新日期：2015-01-05 00:00:00
