Abstract:
OBJECTIVE:To study the effects of skeletal muscle satellite cells implanted into infarcted myocardium on the volume of remnant myocytes. METHODS:Thirty-six adult mongrel canines were divided randomly into implantation group and control group. In the implantation group, skeletal muscle satellite cells taken from the gluteus maximus muscles of the dogs were cultured, proliferated and labeled with 4',6-diamidino-2-phenylindone (DAPI) in vitro. In both groups, a model of acute myocardial infarction was established in every dog. In the implantation group, each dog was injected with M199 solution containing autologous skeletal muscle satellite cells. The dogs in the control group received M199 solution without skeletal muscle satellite cells. The dogs of both groups were killed 2, 4 and 8 weeks after implantation (six dogs in a separate group each time). Both infarcted myocardium and normal myocytes distal from the infracted regions isolated were observed under optical and fluorescent microscope. Their volumes were determined using a confocal microscopy image analysis system and analyzed using SAS. A P < 0.05 was considered significant. RESULTS:A portion of the implanted cells differentiated into muscle fiber with striations and were connected with intercalated discs. Cross-sectional area and cell volume were increased in normal myocardium. Hypertrophy of remnant myocytes in the infarcted site after skeletal muscle cell implantation was much more evident than in the control group. Cross-sectional area, cell area and cell volume differed significantly from those of the control group (P < 0.05). Hypertrophy of the cells occurred predominantly in terms of width and thickness, whereas cell length remained unchanged. CONCLUSION:Skeletal muscle satellite cells implanted into infarct myocardium, could induce the hypertrophy of remnant myocyte cells in the infarcted site and could also aid in the recovery of the contractile force of the infarcted myocardium.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Zhong H,Zhu H,Wei H,Zhang Zkeywords:
subject
Has Abstractpub_date
2003-07-01 00:00:00pages
1088-91issue
7eissn
0366-6999issn
2542-5641journal_volume
116pub_type
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journal_title:Chinese medical journal
pub_type: 杂志文章
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journal_title:Chinese medical journal
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pub_type: 临床试验,杂志文章
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更新日期:2010-09-01 00:00:00
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pub_type: 杂志文章,评审
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doi:
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