Abstract：
:Colorectal cancer results from mutations in components of the Wnt pathway that regulate beta-catenin levels. Dishevelled (Dvl or Dsh) signals downstream of Wnt receptors and stabilizes beta-catenin during cell proliferation and embryonic axis formation. Moreover, Dvl contributes to cytoskeletal reorganization during gastrulation and mitotic spindle orientation during asymmetric cell division. Dvl belongs to a family of eukaryotic signalling proteins that contain a conserved 85-residue module of unknown structure and biological function called the DIX domain. Here we show that the DIX domain mediates targeting to actin stress fibres and cytoplasmic vesicles in vivo. Neighbouring interaction sites for actin and phospholipid are identified between two helices by nuclear magnetic resonance spectroscopy (NMR). Mutation of the actin-binding motif abolishes the cytoskeletal localization of Dvl, but enhances Wnt/beta-catenin signalling and axis induction in Xenopus. By contrast, mutation of the phospholipid interaction site disrupts vesicular association of Dvl, Dvl phosphorylation, and Wnt/beta-catenin pathway activation. We propose that partitioning of Dvl into cytoskeletal and vesicular pools by the DIX domain represents a point of divergence in Wnt signalling.
journal_name
Naturejournal_title
Natureauthors
Capelluto DG,Kutateladze TG,Habas R,Finkielstein CV,He X,Overduin Mdoi
10.1038/nature01056keywords:
subject
Has Abstractpub_date
2002-10-17 00:00:00pages
726-9issue
6908eissn
0028-0836issn
1476-4687pii
nature01056journal_volume
419pub_type
杂志文章相关文献
NATURE文献大全abstract：:Dominant alleles at the agouti locus (A) cause an obesity syndrome in the mouse, as a consequence of ectopic expression of the agouti peptide. This peptide, normally only found in the skin, is a high-affinity antagonist of the melanocyte-stimulating hormone receptor (MC1-R), thus explaining the inhibitory effect of ag...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/385165a0
更新日期：1997-01-09 00:00:00
abstract：:Recently, several groups have initiated studies on cytosolic proteins that bind to isolated secretory vesicle membranes in the presence of Ca2+ in order to identify proteins that may regulate exocytosis. Two major chromaffin granule binding proteins, of molecular weights 32,000 (32K) and 34,000 (34K), were reported to...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/308208a0
更新日期：1984-03-08 00:00:00
abstract：:Olefin chemistry, through pericyclic reactions, polymerizations, oxidations, or reductions, has an essential role in the manipulation of organic matter. Despite its importance, olefin synthesis still relies largely on chemistry introduced more than three decades ago, with metathesis being the most recent addition. Her...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature22307
更新日期：2017-05-11 00:00:00
abstract：:High-pressure transitions are thought to modify hydrogen molecules to a molecular metallic solid and finally to an atomic metal1, which is predicted to have exotic physical properties and the topology of a two-component (electron and proton) superconducting superfluid condensate2,3. Therefore, understanding such trans...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-019-1565-9
更新日期：2019-09-01 00:00:00
abstract：:Immune-challenged vertebrate females transfer specific antibodies to their offspring, but this gratuitous immunity cannot operate in invertebrates. Here we show that constitutive immune defence is enhanced in sexual offspring of the bumble-bee Bombus terrestris L. when the parental colony is immune-challenged. Our fin...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35107138
更新日期：2001-11-29 00:00:00
abstract：:Insulin binding to its receptor leads to negatively cooperative interactions among the receptor sites. Studies with 29 insulin analogues (animal insulins and proinsulin, insulin-like growth factor and chemically modified insulins) which vary 1,000-fold in their affinity for the receptor and in their biological potency...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/273504a0
更新日期：1978-06-15 00:00:00
abstract：:General alarm about genetics continues, fanned by the expectation that what may one day be possible will be a reality tomorrow. Moderation is the most urgent need, but understanding would also help. ...
journal_title：Nature
pub_type： 社论
doi：10.1038/367099a0
更新日期：1994-01-13 00:00:00
abstract：: ...
journal_title：Nature
pub_type： 新闻
doi：10.1038/d41586-018-07631-3
更新日期：2018-12-01 00:00:00
abstract：:Ultrathin ferroelectric materials could potentially enable low-power perovskite ferroelectric tetragonality logic and nonvolatile memories1,2. As ferroelectric materials are made thinner, however, the ferroelectricity is usually suppressed. Size effects in ferroelectrics have been thoroughly investigated in perovskite...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-020-2208-x
更新日期：2020-04-01 00:00:00
abstract：:Excitatory amino acids mediate fast synaptic transmission in the central nervous system through the activation of at least three distinct ionotropic receptors: N-methyl-D-aspartate (NMDA), the alpha-amino-3-hydroxy-5-methyl-isoxasole-4-propionate (AMPA)/quisqualate (QUIS) and the kainate subtypes (for reviews, see ref...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/347765a0
更新日期：1990-10-25 00:00:00
abstract：:Thermodynamics predicts that transmembrane voltage modulates membrane tension and that this will cause movement. The magnitude and polarity of movement is governed by cell stiffness and surface potentials. Here we confirm these predictions using the atomic force microscope to dynamically follow the movement of voltage...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35096578
更新日期：2001-09-27 00:00:00
abstract：:Secretory-protein translocation into the endoplasmic reticulum (ER) is thought to be catalysed by integral membrane proteins. Genetic selections uncovered three Saccharomyces cerevisiae genes (SEC61, SEC62 and SEC63), mutations in which block import of precursor proteins into the ER lumen in vivo and in vitro. The DNA...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/349806a0
更新日期：1991-02-28 00:00:00
abstract：:The question of whether DNA is able to transport electrons has attracted much interest, particularly as this ability may play a role as a repair mechanism after radiation damage to the DNA helix. Experiments addressing DNA conductivity have involved a large number of DNA strands doped with intercalated donor and accep...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/18855
更新日期：1999-04-01 00:00:00
abstract：:The biochemical response to food intake must be precisely regulated. Because ingested sugars and fats can feed into many anabolic and catabolic pathways1, how our bodies handle nutrients depends on strategically positioned metabolic sensors that link the intrinsic nutritional value of a meal with intermediary metaboli...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-018-0849-9
更新日期：2019-02-01 00:00:00
abstract：:Cancer genomics has revealed many genes and core molecular processes that contribute to human malignancies, but the genetic and molecular bases of many rare cancers remains unclear. Genetic predisposition accounts for 5 to 10% of cancer diagnoses in children1,2, and genetic events that cooperate with known somatic dri...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-020-2164-5
更新日期：2020-04-01 00:00:00
abstract：:The interplay between magnetic fields and interacting particles can lead to exotic phases of matter that exhibit topological order and high degrees of spatial entanglement. Although these phases were discovered in a solid-state setting, recent innovations in systems of ultracold neutral atoms-uncharged atoms that do n...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature22811
更新日期：2017-06-21 00:00:00
abstract：:The genes torso (tor) and torso-like (tsl) are two of the Drosophila maternal group genes implicated in a receptor tyrosine kinase signalling pathway that specifies terminal cell fate (reviewed in ref. 3). Loss-of-function mutations in these loci cause an identical phenotype in which pattern elements from the anterior...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/367741a0
更新日期：1994-02-24 00:00:00
abstract：:Behavioural experiences activate the FOS transcription factor in sparse populations of neurons that are critical for encoding and recalling specific events1-3. However, there is limited understanding of the mechanisms by which experience drives circuit reorganization to establish a network of Fos-activated cells. It i...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-020-3031-0
更新日期：2020-12-09 00:00:00
abstract：:Considerable attention has centered recently on the changes in neuron excitability and synaptic efficacy caused by certain biogenic amines and neuropeptides. These neuromodulators act at a wide variety of both central and peripheral targets, and bring about diverse biological results. In sensory pathways, modulation o...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/326793a0
更新日期：1987-04-23 00:00:00
abstract：:Vasopressin (VP) potentiates the effect of corticotropin-releasing factor (CRF) on the secretion of adrenocorticotropic hormone (ACTH) from anterior pituitary cells in vitro, and both CRF and VP have been found in portal blood. These data support the hypothesis that VP acts synergistically with CRF to cause the secret...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/317248a0
更新日期：1985-09-19 00:00:00
abstract：:Abscisic acid (ABA) is a ubiquitous hormone that regulates plant growth, development and responses to environmental stresses. Its action is mediated by the PYR/PYL/RCAR family of START proteins, but it remains unclear how these receptors bind ABA and, in turn, how hormone binding leads to inhibition of the downstream ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature08613
更新日期：2009-12-03 00:00:00
abstract：:Transforming growth factor-alpha (TGF-alpha) is a mitogenically active polypeptide hormone produced by acutely proliferating neoplastic and embryonic tissues. Molecular cloning of TGF-alpha cDNA from human and rat has indicated that this factor is synthesized as part of a larger precursor, proTGF-alpha. An intriguing ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/326883a0
更新日期：1987-04-30 00:00:00
abstract：:Intracellular Ca2+ signalling processes are fundamental to muscle contraction, neurotransmitter release, cell growth and apoptosis. Release of Ca2+ from the intracellular stores is supported by a series of ion channels in sarcoplasmic or endoplasmic reticulum (SR/ER). Among them, two isoforms of the trimeric intracell...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature19767
更新日期：2016-10-27 00:00:00
abstract：:Quantum computation, a paradigm of computing that is completely different from classical methods, benefits from theoretically proved speed-ups for certain problems and can be used to study the properties of quantum systems1. Yet, because of the inherently fragile nature of the physical computing elements (qubits), ach...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-019-1040-7
更新日期：2019-03-01 00:00:00
abstract：:In this Letter, in "About 75% of this reduction is expected to come from emission reductions and the remaining 25% from land use, land-use change and forestry", '25%' should read '1%' and '75%' should read '99%'. In the sentence "The carbon-sink-maximizing portfolio has a small negative effect on annual precipitation ...
journal_title：Nature
pub_type： 已发布勘误
doi：10.1038/s41586-019-1023-8
更新日期：2019-03-01 00:00:00
abstract：:Brain metastasis represents a substantial source of morbidity and mortality in various cancers, and is characterized by high resistance to chemotherapy. Here we define the role of the most abundant cell type in the brain, the astrocyte, in promoting brain metastasis. We show that human and mouse breast and lung cancer...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature18268
更新日期：2016-05-26 00:00:00
abstract：:Activation of a visual pigment molecule to initiate phototransduction requires a minimum energy, Ea, that need not be wholly derived from a photon, but may be supplemented by heat. Theory predicts that absorbance at very long wavelengths declines with the fraction of molecules that have a sufficient complement of ther...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35003242
更新日期：2000-01-13 00:00:00
abstract：:The TATA sequence-binding factor TFIID plays a central role both in promoter activation by RNA polymerase II and other common initiation factors, and in promoter regulation by gene-specific factors. The sequence of yeast TFIID, which seems to be encoded by a single gene, contains interesting structural motifs that are...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/341299a0
更新日期：1989-09-28 00:00:00
abstract：:It has been suggested that the initiation step in mouse skin carcinogenesis involves an alteration in epidermal-differentiation, as mouse basal keratinocytes exposed to initiators resist the arrest of cell growth that is normally associated with the induction of terminal differentiation by calcium ions. The growth of ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/314459a0
更新日期：1985-04-04 00:00:00
abstract：:Melting processes in the deep mantle have important implications for the origin of the deep-derived plumes believed to feed hotspot volcanoes such as those in Hawaii. They also provide insight into how the mantle has evolved, geochemically and dynamically, since the formation of Earth. Melt production in the shallow m...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature11294
更新日期：2012-07-18 00:00:00
