Abstract：
:The formation of axon trajectories requires integration of local adhesive interactions with directional information from attractive and repulsive cues. Here, we show that these two types of information are functionally integrated; activation of the transmembrane receptor Roundabout (Robo) by its ligand, the secreted repulsive guidance cue Slit, inactivates N-cadherin-mediated adhesion. Loss of N-cadherin-mediated adhesion is accompanied by tyrosine phosphorylation of beta-catenin and its loss from the N-cadherin complex, concomitant with the formation of a supramolecular complex containing Robo, Abelson (Abl) kinase and N-cadherin. Local formation of such a receptor complex is an ideal mechanism to steer the growth cone while still allowing adhesion and growth in other directions.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Rhee J,Mahfooz NS,Arregui C,Lilien J,Balsamo J,VanBerkum MFdoi
10.1038/ncb858keywords:
subject
Has Abstractpub_date
2002-10-01 00:00:00pages
798-805issue
10eissn
1465-7392issn
1476-4679pii
ncb858journal_volume
4pub_type
杂志文章abstract：:Different sites of plasma membrane attachment may underlie functional differences between isoforms of Ras. Here we show that palmitoylation and farnesylation targets H-ras to lipid rafts and caveolae, but that the interaction of H-ras with these membrane subdomains is dynamic. GTP-loading redistributes H-ras from raft...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35070050
更新日期：2001-04-01 00:00:00
abstract：:The tumour-suppressor gene ATM, mutations in which cause the human genetic disease ataxia telangiectasia (A-T), encodes a key protein kinase that controls the cellular response to DNA double-strand breaks (DSBs). DNA DSBs caused by ionizing radiation or chemicals result in rapid ATM autophosphorylation, leading to che...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1651
更新日期：2007-11-01 00:00:00
abstract：:The guanosine tri-phosphatase Ran stimulates assembly of microtubule spindles. However, it is not known what aspects of the microtubule cytoskeleton are subject to regulation by Ran in mitosis. Here we show that Ran-GTP stimulates microtubule assembly by increasing the rescue frequency of microtubules three- to eightf...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35060000
更新日期：2001-03-01 00:00:00
abstract：:We have designed new non-peptidic potential inhibitors of gamma-secretase and examined their ability to prevent production of amyloid-beta 40 (Abeta40) and Abeta42 by human cells expressing wild-type and Swedish-mutant beta-amyloid precursor protein (betaAPP). Here we identify three such agents that markedly reduce re...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35074581
更新日期：2001-05-01 00:00:00
abstract：:Protein degradation in eukaryotes often requires the ubiquitin-selective chaperone p97 for substrate recruitment and ubiquitin-chain assembly. However, the physiological relevance of p97, and its role in developmental processes, remain unclear. Here, we discover an unanticipated function for CDC-48/p97 in myosin assem...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1554
更新日期：2007-04-01 00:00:00
abstract：:Activation of the ataxia telangiectasia mutated (ATM) kinase triggers diverse cellular responses to ionizing radiation (IR), including the initiation of cell cycle checkpoints. Histone H2AX, p53 binding-protein 1 (53BP1) and Chk2 are targets of ATM-mediated phosphorylation, but little is known about their roles in sig...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb884
更新日期：2002-12-01 00:00:00
abstract：:Research on genome stability and integrity now extends far beyond the biochemistry of DNA repair to encompass signal transduction pathways that span numerous aspects of cellular life. Derailed genomic integrity pathways can result in debilitating genetic disorders, premature ageing, predisposition to cancer and degene...
journal_title：Nature cell biology
pub_type：
doi：10.1038/ncb1004-923
更新日期：2004-10-01 00:00:00
abstract：:The seminal observation that mechanical signals can elicit changes in biochemical signalling within cells, a process commonly termed mechanosensation and mechanotransduction, has revolutionized our understanding of the role of cell mechanics in various fundamental biological processes, such as cell motility, adhesion,...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb3524
更新日期：2017-05-31 00:00:00
abstract：:The position of the mitotic spindle controls the plane of cell cleavage and determines whether polarized cells divide symmetrically or asymmetrically. In animals, an evolutionarily conserved pathway of LIN-5 (homologues: Mud and NuMA), GPR-1/2 (homologues: Pins, LGN, AGS-3) and Gα mediates spindle positioning, and act...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2315
更新日期：2011-08-21 00:00:00
abstract：:Regulated activation of integrins is critical for cell adhesion, motility and tissue homeostasis. Talin and kindlins activate β1-integrins, but the counteracting inhibiting mechanisms are poorly defined. We identified SHARPIN as an important inactivator of β1-integrins in an RNAi screen. SHARPIN inhibited β1-integrin ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2340
更新日期：2011-09-25 00:00:00
abstract：:Extracellular signal-regulated kinase (ERK) controls fundamental cellular functions, including cell fate decisions. In PC12, cells shifting ERK activation from transient to sustained induces neuronal differentiation. As ERK associates with both regulators and effectors, we hypothesized that the mechanisms underlying t...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1994
更新日期：2009-12-01 00:00:00
abstract：:Chaperone proteins are thought to promote the correct folding and assembly of newly synthesized proteins and to facilitate restoration of the folded state under environmental conditions that favour protein denaturation. They are among the most ubiquitous and highly conserved of all proteins. The eukaryotic endoplasmic...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1001-891
更新日期：2001-10-01 00:00:00
abstract：:Centromeres are specialized chromosomal domains that direct kinetochore assembly during mitosis. CENP-A (centromere protein A), a histone H3-variant present exclusively in centromeric nucleosomes, is thought to function as an epigenetic mark that specifies centromere identity. Here we identify the essential centromere...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1899
更新日期：2009-07-01 00:00:00
abstract：:Blocking mitotic progression has been proposed as an attractive therapeutic strategy to impair proliferation of tumour cells. However, how cells survive during prolonged mitotic arrest is not well understood. We show here that survival during mitotic arrest is affected by the special energetic requirements of mitotic ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3231
更新日期：2015-10-01 00:00:00
abstract：:Phosphorylation networks intimately regulate mechanisms of response to therapies. Mapping the phospho-catalytic profile of kinases in cells or tissues remains a challenge. Here, we introduce a practical high-throughput system to measure the enzymatic activity of kinases using biological peptide targets as phospho-sens...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-019-0328-z
更新日期：2019-06-01 00:00:00
abstract：:Given the important role of the hypothalamus in regulating feeding and metabolism, there has been considerable interest in a possible function for hypothalamic stem cells in modulating body weight in health and disease. Mice given a high-fat diet develop inflammation in the hypothalamus and lose key types of neurons. ...
journal_title：Nature cell biology
pub_type： 新闻
doi：10.1038/ncb2599
更新日期：2012-10-01 00:00:00
abstract：:The retinoblastoma protein (pRB) negatively regulates the progression from G1 to S phase of the cell cycle, in part, by repressing E2F-dependent transcription. pRB also possesses E2F-independent functions that contribute to cell-cycle control--for example, during pRB-mediated cell-cycle arrest pRB associates with Skp2...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1532
更新日期：2007-02-01 00:00:00
abstract：:Chromophore-assisted laser inactivation (CALI) is a light-mediated technique used to selectively inactivate proteins within cells. Here, we demonstrate that GFP can be used as a CALI reagent to locally inactivate proteins in living cells. We show that focused laser irradiation of EGFP-alpha-actinin expressed in Swiss ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb772
更新日期：2002-04-01 00:00:00
abstract：:FoxG1 is an evolutionarily conserved, winged-helix transcriptional repressor that maintains progenitor cells in the vertebrate forebrain. How the activity of FoxG1 is regulated is not known. Here, we report that in the developing Xenopus and mouse forebrain, FoxG1 is nuclear in progenitor cells but cytoplasmic in diff...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1573
更新日期：2007-05-01 00:00:00
abstract：:The metazoan nuclear pore complex (NPC) disassembles during mitosis, and many of its constituents distribute onto spindles and kinetochores, including the Nup107-160 sub-complex. We have found that Nup107-160 interacts with the gamma-tubulin ring complex (gamma-TuRC), an essential and conserved microtubule nucleator, ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2016
更新日期：2010-02-01 00:00:00
abstract：:Most invasive solid tumours display predominantly collective invasion, in which groups of cells invade the peritumoral stroma while maintaining cell-cell contacts. As the concepts and experimental models for functional analysis of collective cancer cell invasion are rapidly developing, we propose a framework for addre...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb2548
更新日期：2012-08-01 00:00:00
abstract：:In some organs, adult stem cells are uniquely poised to serve as cancer cells of origin. It is unclear, however, whether tumorigenesis is influenced by the activation state of the adult stem cell. Hair follicle stem cells (HFSCs) act as cancer cells of origin for cutaneous squamous cell carcinoma and undergo defined c...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2889
更新日期：2014-01-01 00:00:00
abstract：:Examination of the tumour suppressor adenomatous polyposis coli (APC) has shown that it may be multifunctional. Recent work has demonstrated dynamic interactions of APC with the microtubule cytoskeleton, supporting the idea that APC has an important function in cell migration. ...
journal_title：Nature cell biology
pub_type： 新闻,评审
doi：10.1038/35008685
更新日期：2000-04-01 00:00:00
abstract：:Formins have been implicated in the regulation of cytoskeletal structure in animals and fungi. Here we show that the formins Bni1 and Bnr1 of budding yeast stimulate the assembly of actin filaments that function as precursors to tropomyosin-stabilized cables that direct polarized cell growth. With loss of formin funct...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb718
更新日期：2002-01-01 00:00:00
abstract：:In the version of this Article originally published, ref. 52 was incorrectly only attributed to its corresponding author, Fre, S., and an older title was used. The correct citation should have been: Lilja, A. M. et al. Clonal analysis of Notch1-expressing cells reveals the existence of unipotent stem cells that retain...
journal_title：Nature cell biology
pub_type： 已发布勘误
doi：10.1038/s41556-018-0164-6
更新日期：2018-09-01 00:00:00
abstract：:Actin dynamics is a tightly regulated process involved in various cellular events including biogenesis of clathrin-coated, AP-1 (adaptor protein 1)-coated transport carriers connecting the trans-Golgi network (TGN) and the endocytic pathway. However, the mechanisms coordinating coat assembly, membrane and actin remode...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2034
更新日期：2010-04-01 00:00:00
abstract：:Once considered to be contained, infectious diseases of bacterial origin are now making a comeback. A lack of innovative therapies and the appearance of drug-resistant pathogens are becoming increasingly serious problems. A better understanding of pathogen-host interactions at the cellular and molecular levels is nece...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/15620
更新日期：1999-11-01 00:00:00
abstract：:The tumour stroma is an active participant during cancer progression. Stromal cells promote tumour progression and metastasis through multiple mechanisms including enhancing tumour invasiveness and angiogenesis, and suppressing immune surveillance. We report here that miR-126/miR-126(*), a microRNA pair derived from a...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2690
更新日期：2013-03-01 00:00:00
abstract：:Although many genes that lead to different types of cancer when mutated have been identified, the cells that initiate tumour formation following accumulation of these mutations have, until recently, remained elusive. This review explores how mouse genetic lineage-tracing experiments that allow the expression of oncoge...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb2657
更新日期：2013-02-01 00:00:00
abstract：:When co-translationally inserted into endoplasmic reticulum (ER) membranes, newly synthesized proteins encounter the lumenal environment of the ER, which contains chaperone proteins that facilitate the folding reactions necessary for protein oligomerization, maturation and export from the ER. Here we show, using a tem...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35010558
更新日期：2000-05-01 00:00:00
