Abstract：
:Crk is a signaling adaptor protein which is mostly composed of SH2 and SH3 domains, and has been shown to play a pivotal role in cell proliferation, differentiation, and migration. Because Crk was originally isolated as an avian sarcoma virus CT10 encoding oncoprotein v-Crk, we examined a potential role for c-Crk in the carcinogenesis of human cancers. First, to analyze gene mutations of c-Crk, we isolated a human bacterial artificial chromosome clone containing Crk genome and exon/intron structures. However, polymerase chain reaction-single strand conformation polymorphism methods failed to show any genomic mutations in the Crk exon which could be related to carcinogenesis. Second, immunohistochemical analysis of c-Crk-II demonstrated that the levels of c-Crk-II were significantly elevated in most of the tumors, particularly in the colon and lung cancers. Furthermore, immunoblot analysis using human lung cancer cell lines revealed that the expression levels of c-Crk-II were correlated to growth rates of cells. The elevated expression levels of c-Crk-II might be related to the development of human cancers.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Nishihara H,Tanaka S,Tsuda M,Oikawa S,Maeda M,Shimizu M,Shinomiya H,Tanigami A,Sawa H,Nagashima Kdoi
10.1016/s0304-3835(01)00763-7keywords:
subject
Has Abstractpub_date
2002-06-06 00:00:00pages
55-61issue
1eissn
0304-3835issn
1872-7980pii
S0304383501007637journal_volume
180pub_type
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