Hepatitis B virus PreS1 facilitates hepatocellular carcinoma development by promoting appearance and self-renewal of liver cancer stem cells.


:Hepatitis B virus (HBV) is a major etiologic agent of hepatocellular carcinoma (HCC). However, the molecular mechanism by which HBV infection contributes to HCC development is not fully understood. Here, we initially showed that HBV stimulates the production of cancer stem cells (CSCs)-related markers (CD133, CD117 and CD90) and CSCs-related genes (Klf4, Sox2, Nanog, c-Myc and Oct4) and facilitates the self-renewal of CSCs in human hepatoma cells. Cellular and clinical studies revealed that HBV facilitates hepatoma cell growth and migration, enhances white blood cell (WBC) production in the sera of patients, stimulates CD133 and CD117 expression in HCC tissues, and promotes the CSCs generation of human hepatoma cells and clinical cancer tissues. Detailed studies revealed that PreS1 protein of HBV is required for HBV-mediated CSCs generation. PreS1 activates CD133, CD117 and CD90 expression in normal hepatocyte derived cell line (L02) and human hepatoma cell line (HepG2 and Huh-7); facilitates L02 cells migration, growth and sphere formation; and finally enhances the abilities of L02 cells and HepG2 cells to induce tumorigeneses in nude mice. Thus, PreS1 acts as a new oncoprotein to play a key role in the appearance and self-renewal of CSCs during HCC development.


Cancer Lett


Cancer letters


Liu Z,Dai X,Wang T,Zhang C,Zhang W,Zhang W,Zhang Q,Wu K,Liu F,Liu Y,Wu J




Has Abstract


2017-08-01 00:00:00












  • Activation of lymphocytes of normal and tumor bearing mice by mangiferin, a naturally occurring glucosylxanthone.

    abstract::Mangiferin, a naturally occurring glucosylxanthone, was assessed for its immunomodulatory potential. The phytochemical induced extensive in vitro proliferation of murine splenocytes and thymocytes at the doses of 5-40 micrograms ml-1. Suppression of the proliferative response of the cells was observed with higher dose...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Chattopadhyay U,Das S,Guha S,Ghosal S

    更新日期:1987-11-01 00:00:00

  • mTOR inhibition by everolimus counteracts VEGF induction by sunitinib and improves anti-tumor activity against gastric cancer in vivo.

    abstract::VEGF receptor blockage has been reported to increase serum VEGF. We hypothesized that mTOR inhibition by everolimus counteracts VEGF induction by sunitinib resulting in an improved anti-tumor activity of sunitinib. In vitro, sunitinib in combination with everolimus did not outperform the respective monotherapies. In v...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Fuereder T,Jaeger-Lansky A,Hoeflmayer D,Preusser M,Strommer S,Cejka D,Koehrer S,Crevenna R,Wacheck V

    更新日期:2010-10-28 00:00:00

  • Inhibition of angiogenesis by a novel small peptide consisting of the active fragments of platelet factor-4 and vasostatin.

    abstract::Inhibition of angiogenesis provides a potential anti-cancer therapy. Here we describe a novel recombinant peptide composed of active fragments of two distinct angiogenesis inhibitors, platelet factor-4 (C-13 fragment) and vasostatin (N-terminal amino acids 135-164) with a nonpolar peptide bridge of Gly-Pro-Gly. This f...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Li X,Jiang L,Wang Y,Xiao Y,Huang Y,Yao Q,Yang Y,Wu X

    更新日期:2007-10-18 00:00:00

  • Epstein-Barr virus infection leads to partial phenotypic reversion of terminally differentiated malignant B cells.

    abstract::The B cell lymphomas associated with Epstein-Barr virus (EBV) are not limited to any specific stage of B cell differentiation but covers widely different B cell phenotypes. In vitro infection of the virus negative tumors with a recombinant EBV strain has provided important insights into virus-tumor interaction. Here, ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Anastasiadou E,Vaeth S,Cuomo L,Boccellato F,Vincenti S,Cirone M,Presutti C,Junker S,Winberg G,Frati L,Wade PA,Faggioni A,Trivedi P

    更新日期:2009-11-01 00:00:00

  • Morphological detection of cell kinetics and progesterone receptor expression during growth and regression of pregnancy-dependent mammary tumors of GRS/A mice.

    abstract::Cell kinetics (cell proliferation and cell death) and the expression of progesterone receptor (PgR) during growth and regression of pregnancy-dependent mammary tumors (PDMT) of female GRS/A mice were investigated on the histologic level. Cell proliferation was determined using monoclonal anti-proliferating cell nuclea...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Yang J,Senzaki H,Nambu H,Tsubura A

    更新日期:1996-08-23 00:00:00

  • Optimized dissociation protocol for isolating human glioma stem cells from tumorspheres via fluorescence-activated cell sorting.

    abstract::Fluorescence-activated cell sorting (FACS) based on the surface marker CD133 is the most common method for isolating glioma stem cells (GSCs) from heterogeneous glioma cell populations. Optimization of this method will have profound implications for the future of GSC research. Five commonly used digestion reagents, Li...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Lv D,Ma QH,Duan JJ,Wu HB,Zhao XL,Yu SC,Bian XW

    更新日期:2016-07-10 00:00:00

  • Preclinical comparison of regorafenib and sorafenib efficacy for hepatocellular carcinoma using multimodality molecular imaging.

    abstract::Sorafenib has been used as a clinical targeted therapy for hepatocellular carcinoma (HCC) for more than a decade. In 2017, regorafenib was approved for HCC treatment and has since been reported to prolong the survival of advanced HCC patients after treatment failure with sorafenib. However, there has been no direct sy...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Liu S,Du Y,Ma H,Liang Q,Zhu X,Tian J

    更新日期:2019-07-01 00:00:00

  • Effect of inducible FHIT and p53 expression in the Calu-1 lung cancer cell line.

    abstract::Loss of FHIT expression and p53 mutations are critical events in the early stages of lung carcinogenesis. The restoration of Fhit function in FHIT-negative cancer cells has been reported to cause tumour suppression by inhibition of cell proliferation and/or activation of apoptotic pathways. However, the studies design...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Cavazzoni A,Galetti M,Fumarola C,Alfieri RR,Roz L,Andriani F,Carbognani P,Rusca M,Sozzi G,Petronini PG

    更新日期:2007-02-08 00:00:00

  • The NLRP3 inflammasome: Role in metabolic disorders and regulation by metabolic pathways.

    abstract::Inflammasomes are large multimolecular complexes present in the cytosol of stimulated immune cells; they mediate the activation of caspase-1, leading to cellular pyroptosis. So far, a variety of studies on inflammasomes have emerged, and the best-studied is the NLRP3 inflammasome that is involved in many inflammatory ...

    journal_title:Cancer letters

    pub_type: 杂志文章,评审


    authors: Jiang D,Chen S,Sun R,Zhang X,Wang D

    更新日期:2018-04-10 00:00:00

  • Fluorescence photography as a diagnostic method for oral cancer.

    abstract::This study was carried out to evaluate the diagnostic utility of autofluorescence photography for oral mucosal lesions. The materials consisted of 15 chemically-induced lesions containing carcinomas in 15 hamsters, and 32 oral lesions in 30 patients. In the animal models, orange fluorescence was detected in all squamo...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Onizawa K,Saginoya H,Furuya Y,Yoshida H

    更新日期:1996-11-12 00:00:00

  • Signal-dependent regulation of gene expression as a target for cancer treatment: inhibiting p38alpha in colorectal tumors.

    abstract::In the last year, several evidences indicated that pharmacological manipulation of relevant signaling pathways could selectively affect gene expression to influence cell fate. These findings render of extreme importance the elucidation of how external stimuli are transduced to mediate chromatin modifications, resultin...

    journal_title:Cancer letters

    pub_type: 杂志文章,评审


    authors: Chiacchiera F,Simone C

    更新日期:2008-06-28 00:00:00

  • Postnatal efficacy of Momordica charantia peel, pulp, seed and whole fruit extract in the detoxication pathway of suckling neonates and lactating mice.

    abstract::The present study evaluates the postnatal efficacy of Momordica charantia peel, pulp, seed and whole fruit extract via assessing the modulation in the biotransformation system enzymes of suckling neonates and lactating mice. The peel, seed or whole fruit extract of Momordica (100 microl/animal/day) independently induc...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Singh A,Singh SP,Bamezai R

    更新日期:1998-01-09 00:00:00

  • The actin-bundling protein TRIOBP-4 and -5 promotes the motility of pancreatic cancer cells.

    abstract::TRIOBP isoforms 4 and 5 (TRIOBP-4/-5) are an actin-bundling protein associated with hearing loss. Here, we showed that TRIOBP-4/-5 was up-regulated in human pancreatic carcinoma cells. Knockdown of TRIOBP-4/-5 led to a loss of filopodia and a decrease in cell motility. Confocal microscopy showed that re-expression of ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Bao J,Wang S,Gunther LK,Kitajiri S,Li C,Sakamoto T

    更新日期:2015-01-28 00:00:00

  • TACC3 promotes epithelial-mesenchymal transition (EMT) through the activation of PI3K/Akt and ERK signaling pathways.

    abstract::Transforming acidic coiled-coil protein 3 (TACC3) is a member of the TACC family, essential for mitotic spindle dynamics and centrosome integrity during mitosis. Mounting evidence suggests that deregulation of TACC3 is associated with various types of human cancer. However, the molecular mechanisms by which TACC3 cont...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Ha GH,Park JS,Breuer EK

    更新日期:2013-05-10 00:00:00

  • Decreased [20-3H]phorbol-12,13-dibutyrate binding to phospholipase C pretreated epidermal cells.

    abstract::Incubation of mouse epidermal cells (HEL-37) with phospholipase C (Clostridium perfringens) caused about a 50% decrease in the specific binding of [20-3H]phorbol-12,13-dibutyrate. Phospholipase C caused a decrease in the apparent number of binding sites from 2.86 X 10(5) to 1.21 X 10(5) per cell with little effect on ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Jones MJ,Murray AW

    更新日期:1983-05-01 00:00:00

  • BRCA1-2 mutations in breast cancer: identification of nine new variants of BRCA1-2 genes in a population from central Western Spain.

    abstract::We carried out a mutational analysis of BRCA1 and BRCA2 genes in 103 individuals from a population in Western Central Spain and we identified nine new variants: two truncating mutations in BRCA2 [2604C>A (Y792X), 8873del4], three missense mutations in BRCA2 [677A>G (H150R), 958G>A (D224N) and 3398A>G (K1057R], and fou...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Salazar R,Cruz-Hernandez JJ,Sanchez-Valdivieso E,Rodriguez CA,Gomez-Bernal A,Barco E,Fonseca E,Portugal T,Gonzalez-Sarmiento R

    更新日期:2006-02-20 00:00:00

  • Gut microbiota: A new piece in understanding hepatocarcinogenesis.

    abstract::The gut microbiota forms a symbiotic relationship with the host and benefits the body in many critical aspects of life. However, immune system defects, alterations in the gut microbiota and environmental changes can destroy this symbiotic relationship and may lead to diseases, including cancer. Due to the anatomic and...

    journal_title:Cancer letters

    pub_type: 杂志文章,评审


    authors: Zhou A,Tang L,Zeng S,Lei Y,Yang S,Tang B

    更新日期:2020-04-01 00:00:00

  • Proteomics discovery of radioresistant cancer biomarkers for radiotherapy.

    abstract::Radiotherapy (RT) is one of the most important strategies in cancer treatment. Radioresistance is a major challenge to RT and results in locoregional recurrence and metastasis. Thus, there is a great interest in investigating biomarkers to distinguish radiosensitive from radioresistant (RR) cancer patients. The develo...

    journal_title:Cancer letters

    pub_type: 杂志文章,评审


    authors: Chang L,Graham P,Hao J,Bucci J,Malouf D,Gillatt D,Li Y

    更新日期:2015-12-28 00:00:00

  • RSPO2 suppresses colorectal cancer metastasis by counteracting the Wnt5a/Fzd7-driven noncanonical Wnt pathway.

    abstract::R-spondins play critical roles in development, stem cell survival, and tumorigenicity by modulating Wnt/β-catenin signaling; however, the role of R-spondins in noncanonical Wnt signaling regulation remains largely unknown. We demonstrate here that R-spondin 2 (RSPO2) has an inhibitory effect on colorectal cancer (CRC)...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Dong X,Liao W,Zhang L,Tu X,Hu J,Chen T,Dai X,Xiong Y,Liang W,Ding C,Liu R,Dai J,Wang O,Lu L,Lu X

    更新日期:2017-08-28 00:00:00

  • Coding polymorphisms in Casp5, Casp8 and DR4 genes may play a role in predisposition to lung cancer.

    abstract::Apoptosis plays a role in the elimination of DNA-damaged cells thus protecting the host from cancer development. Some data indicate that normal variations within the sequence of apoptotic genes may lead to suboptimal apoptotic capacity and therefore increased cancer risk. We tested 19 coding apoptotic gene SNPs in 2-s...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Ulybina YM,Kuligina ES,Mitiushkina NV,Rozanov ME,Ivantsov AO,Ponomariova DN,Togo AV,Levchenko EV,Shutkin VA,Brenister SI,Devilee P,Zhivotovsky B,Hirvonen A,Imyanitov EN

    更新日期:2009-06-18 00:00:00

  • Effect of cis-unsaturated fatty acids on Meth-A ascitic tumour cells in vitro and in vivo.

    abstract::Earlier studies performed both by us and by others have demonstrated that some n-3 and n-6 fatty acids can inhibit the growth of tumour cells in vitro. Though studies done with various types of oils rich in n-3 and n-6 fatty acids did show that the tumour incidence and growth can be modified, there were relatively few...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Ramesh G,Das UN

    更新日期:1998-01-30 00:00:00

  • Sensitization of cerebral tissue in nude mice with photodynamic therapy induces ADAM17/TACE and promotes glioma cell invasion.

    abstract::In the present study, we tested the hypothesis that a mild cerebral tissue injury promotes subsequent glioma invasion via activation of the ADAM17-EGFR-PI3K-Akt pathway. Mild injury was induced by photodynamic therapy (PDT), which employs tissue-penetrating laser light exposure following systemic administration of a t...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Zheng X,Jiang F,Katakowski M,Zhang X,Jiang H,Zhang ZG,Chopp M

    更新日期:2008-07-08 00:00:00

  • Endothelin-1 axis fosters YAP-induced chemotherapy escape in ovarian cancer.

    abstract::The majority of ovarian cancer (OC) patients recur with a platinum-resistant disease. OC cells activate adaptive resistance mechanisms that are only partially described. Here we show that OC cells can adapt to chemotherapy through a positive-feedback loop that favors chemoresistance. In platinum-resistant OC cells we ...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Tocci P,Cianfrocca R,Sestito R,Rosanò L,Di Castro V,Blandino G,Bagnato A

    更新日期:2020-11-01 00:00:00

  • Further investigations into the effect of non-benzo ring bay-region methyl substituents on tumor-initiating activity of polycyclic hydrocarbons.

    abstract::The effect of a methyl substituent at the non-benzo ring bay-region position of benzo[e]pyrene (B[e]P), cholanthrene (CA) and dibenz[a,j]anthracene (DB[a,j]A) on skin tumor-initiating activity was examined. A methyl substituent at the 1-carbon of B[e]P enhanced tumor-initiating activity of the parent compound (0.18 vs...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Sawyer TW,Chang K,Harvey RG,DiGiovanni J

    更新日期:1987-09-01 00:00:00

  • Arsenic trioxide amplifies cisplatin toxicity in human tubular cells transformed by HPV-16 E6/E7 for further therapeutic directions in renal cell carcinoma.

    abstract::Human papillomavirus (HPV) DNA integrations may affect therapeutic responses in cancers through ATM network-related DNA damage response (DDR). We studied whether cisplatin-induced DDR was altered in human HK-2 renal tubular cells immortalized by HPV16 E6/E7 genes. Cytotoxicity assays utilized thiazolyl blue dye and DD...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Dogra S,Bandi S,Viswanathan P,Gupta S

    更新日期:2015-01-28 00:00:00

  • Intracellular localization and concentration as well as photodynamic effects of benzoporphyrin derivative monoacid ring A in four types of rodent tumor cells.

    abstract::The relative sensitivities of different tumor cells to photodynamic therapy (PDT) with benzoporphyrin derivative monoacid ring A (BPD-MA) were compared in the four tumor cells. A good correlation was observed between the cell survival at 0.1 microg/ml of BPD-MA and sensitizer uptake/10(6) cells (r = -0.99) or the plat...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Osaki T,Takagi S,Hoshino Y,Okumura M,Fujinaga T

    更新日期:2006-11-18 00:00:00

  • Tri-n-butyltin chloride promotes morphological transformation and induces proliferin expression in C3H10T1/2 cells.

    abstract::Transcripts from the murine gene family proliferin, which are increased by a wide assortment of chemical promoters of C3H10T1/2 cell morphological transformation, were shown to be induced by tri-n-butyltin chloride at concentrations above 50 nM. Two-stage transformation assays, with 3-methylcholanthrene as inducer and...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Parfett CL,Pilon R

    更新日期:1993-07-30 00:00:00

  • Regulation of the intestinal mucin MUC2 gene expression in vivo: evidence for the role of promoter methylation.

    abstract::In the present work we investigated the in vivo regulation of the mucin gene MUC2, which is overexpressed in all mucinous colorectal carcinomas. The inhibition of methylation by 5-azadeoxycytidine induces de novo expression of MUC2 in the colon carcinoma cell line COLO 205. The expression is retained in xenograft tiss...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Gratchev A,Siedow A,Bumke-Vogt C,Hummel M,Foss HD,Hanski ML,Kobalz U,Mann B,Lammert H,Mansmann U,Stein H,Riecken EO,Hanski C

    更新日期:2001-07-10 00:00:00

  • Fibronectin promotes cell proliferation and invasion through mTOR signaling pathway activation in gallbladder cancer.

    abstract::Fibronectin (FN), a heterodimeric glycoprotein overexpressed in several types of tumors, has been implicated in cancer progression via the activation of integrin-mediated pro-oncogenic pathways. The FN level in human bile fluid is dramatically increased in malignant biliary diseases; however, FN expression and its bio...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Cao Y,Liu X,Lu W,Chen Y,Wu X,Li M,Wang XA,Zhang F,Jiang L,Zhang Y,Hu Y,Xiang S,Shu Y,Bao R,Li H,Wu W,Weng H,Yen Y,Liu Y

    更新日期:2015-05-01 00:00:00

  • Regulation of death promoter Bak expression by cell density and 17 beta-estradiol in MCF-7 cells.

    abstract::In the current study we examined the regulation of Bak, a death promoter of an apoptotic pathway, in the human breast cancer cell line MCF-7. We observed a time-dependent increase in both Bak mRNA and protein levels which appeared to correlate well with the increase in cell density. We also found that treatment of cel...

    journal_title:Cancer letters

    pub_type: 杂志文章


    authors: Leung LK,Do L,Wang TT

    更新日期:1998-02-13 00:00:00