Drug-resistant human bladder-cancer cells are more sensitive to adenovirus-mediated wild-type p53 gene therapy compared to drug-sensitive cells.


:We investigated the therapeutic potential and molecular mechanism of adenovirus-mediated wt p53 gene therapy for drug-resistant human bladder cancers. KK47, a human bladder-cancer cell line, along with the drug-resistant sublines KK47/DDP10, KK47/DDP20 (cisplatin-resistant) and KK47/ADM (doxorubicin-resistant) were used for the experiments. All 4 KK47 cell lines had genetically normal p53 genes. Using an in vitro cytotoxicity assay, the drug-resistant cell lines were more sensitive to Ad-CMV-p53 cell killing than the KK47 parental cell line. Ad-CMV-p53 induced higher levels of p53 protein and mRNA in the drug-resistant cell lines than in the parental cell line and, consequently, higher levels of p21 and Bax mRNA, which resulted in higher percentages of G(1) cell-cycle arrest and apoptosis. The higher efficiencies of adenoviral gene transfer in the drug-resistant cell lines were confirmed by X-gal staining after infection with Ad-CMV-beta-gal. In conclusion, adenovirus-mediated wt p53 gene therapy was more effective in the drug-resistant bladder-cancer cell lines than in the drug-sensitive bladder-cancer cell line.


Int J Cancer


Shirakawa T,Sasaki R,Gardner TA,Kao C,Zhang ZJ,Sugimura K,Matsuo M,Kamidono S,Gotoh A





Has Abstract


2001-10-15 00:00:00














  • Distribution of the transferrin receptor in normal human fibroblasts and fibrosarcoma cells.

    abstract::Transferrin is required for proliferation of most cells in culture. This effect is presumably mediated by the binding of transferrin to its receptor, a surface glycoprotein which is preferentially expressed by actively growing cells. Here we show that normal human fibroblasts cultured in serum, and other media contain...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ekblom P,Thesleff I,Lehto VP,Virtanen I

    更新日期:1983-01-15 00:00:00

  • DNA methylation patterns in blood of patients with colorectal cancer and adenomatous colorectal polyps.

    abstract::Colorectal cancer (CRC) screening rates are currently suboptimal. Blood-based screening could improve rates of earlier detection for CRC and adenomatous colorectal polyps. In this study, we evaluated the feasibility of plasma-based detection of early CRC and adenomatous polyps using array-mediated analysis methylation...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Cassinotti E,Melson J,Liggett T,Melnikov A,Yi Q,Replogle C,Mobarhan S,Boni L,Segato S,Levenson V

    更新日期:2012-09-01 00:00:00

  • Serological survey and virus isolation of simian T-cell leukemia/T-lymphotropic virus type I (STLV-I) in non-human primates in their native countries.

    abstract::Infection with a simian retrovirus (STLV-I) closely related to human T-lymphotropic virus type I (HTLV-I) was investigated in non-human primates living in their native countries in Africa and Asia. Serum antibodies cross-reacting with HTLV-I antigens were detected in 85 of 567 non-human primates of 30 species. Seropos...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ishikawa K,Fukasawa M,Tsujimoto H,Else JG,Isahakia M,Ubhi NK,Ishida T,Takenaka O,Kawamoto Y,Shotake T

    更新日期:1987-08-15 00:00:00

  • Production of T-cell lines with inhibitory or stimulatory activity against syngeneic tumors in vivo. A preliminary report.

    abstract::We obtained Thy-I-positive cells directly from growing methylcholanthrene-induced (MCA-1510) sarcomas using fluorescence-activated cell sorting, then cultured these lymphocytes in medium containing Interleukin-2 and tested their activity in vivo against various MCA-sarcoma lines with the Winn assay. We found that cult...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Mulé JJ,Forstrom JW,George E,Hellström I,Hellström KE

    更新日期:1981-11-15 00:00:00

  • Influence of cell-free tumour-associated antigen preparations on the development of immunity to chemically induced rat tumours.

    abstract::Inbred rats were treated with extranuclear tumour membrane fractions or 3 M KCl-solubilized extracts of two antigenically distinct 3-methylcholanthrene-induced sarcomas, Mc7 and Mc57. The rats developed tumour-specific humoral antibody responses, but were not immune to tumour challenge. Moreover, they were unresponsiv...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Embleton MJ

    更新日期:1976-11-15 00:00:00

  • A potential role for aspirin in the prevention and treatment of cholangiocarcinoma.

    abstract::Cholangiocarcinoma is the second most common primary hepatic cancer, with a rising incidence worldwide. Owing to late diagnosis and limited treatment options, the prognosis for cholangiocarcinoma remains dismal, compelling a search for new treatments. As aspirin exhibits a well-supported chemopreventive effect on comm...

    journal_title:International journal of cancer

    pub_type: 杂志文章,评审


    authors: Shen X,Shen X

    更新日期:2021-03-15 00:00:00

  • High in vitro-in vivo correlation of drug response using sponge-gel-supported three-dimensional histoculture and the MTT end point.

    abstract::The in vitro sponge-gel-supported three-dimensional histoculture chemosensitivity assay (Hoffman assay) allows the in vivo-like culture of human tumors. In this study, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H tetrazolium bromide (MTT) end point was applied to the Hoffman assay in an attempt to increase in vitr...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Furukawa T,Kubota T,Watanabe M,Takahara T,Yamaguchi H,Takeuchi T,Kase S,Kodaira S,Ishibiki K,Kitajima M

    更新日期:1992-05-28 00:00:00

  • Type I IFNs induce anti-tumor polarization of tumor associated neutrophils in mice and human.

    abstract::The importance of tumor associated neutrophils (TANs) in cancer development is in the meantime well established. Numerous of clinical data document the adverse prognostic effects of neutrophil infiltration in solid tumors. However, certain tumor therapies need functional neutrophils to be effective, suggesting altered...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Andzinski L,Kasnitz N,Stahnke S,Wu CF,Gereke M,von Köckritz-Blickwede M,Schilling B,Brandau S,Weiss S,Jablonska J

    更新日期:2016-04-15 00:00:00

  • Premature chromosome condensation: evidence for in vivo cell fusion in human malignant tumours.

    abstract::Premature chromosome condensation (PCC) was studied in direct preparations of tissues from patients with haematological diseases and carcinomas of various histological types. PCC was found in 6 out of 166 malignancies (128 haematological cases, 35 carcinomas and 3 malignant effusions) analysed with the GTG-technique. ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kovacs G

    更新日期:1985-12-15 00:00:00

  • HPP1-mediated tumor suppression requires activation of STAT1 pathways.

    abstract::HPP1 is a recently discovered gene that is epigenetically silenced in a number of tumor types, suggesting a potential role as a tumor suppressor. However, whether HPP1 has tumor suppressor activity is not clearly known. We have sought to investigate the effects of HPP1 on tumor growth and survival and to identify sign...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Elahi A,Zhang L,Yeatman TJ,Gery S,Sebti S,Shibata D

    更新日期:2008-04-01 00:00:00

  • Clonal heterogeneity in sporadic melanomas as revealed by loss-of-heterozygosity analysis.

    abstract::The major obstacle preventing effective treatment of melanoma is the biological heterogeneity of tumor cells. This study was performed to determine clonal genetic heterogeneity within primary melanoma and the evolution of these heterogeneous sub-clones during disease progression. DNA samples were obtained from 44 morp...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Takata M,Morita R,Takehara K

    更新日期:2000-02-15 00:00:00

  • APE1 genotype and risk of bladder cancer: evidence for effect modification by smoking.

    abstract::Apurinic/apyrimidinic (AP) sites are common mutagenic and cytotoxic DNA lesions that arise from the loss of normal bases. APE1, the major AP endonuclease of human cells, plays a central role in the repair of AP sites through both its endonuclease and phosphodiesterase activities. A common APE1 polymorphism, a T-->G tr...

    journal_title:International journal of cancer

    pub_type: 杂志文章,多中心研究


    authors: Terry PD,Umbach DM,Taylor JA

    更新日期:2006-06-15 00:00:00

  • Clusters of chromosomal imbalances in thymic epithelial tumours are associated with the WHO classification and the staging system according to Masaoka.

    abstract::Using comparative genomic hybridisation, we investigated chromosomal imbalances in 28 cases of thymic epithelial neoplasms including type A, B2, B3, the A component of type AB and different subtypes of type C thymoma. To identify different patterns of chromosomal aberrations associated with the biological behaviour an...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Penzel R,Hoegel J,Schmitz W,Blaeker H,Morresi-Hauf A,Aulmann S,Hecker E,Mechtersheimer G,Otto HF,Rieker RJ

    更新日期:2003-07-01 00:00:00

  • MLN64 exhibits homology with the steroidogenic acute regulatory protein (STAR) and is over-expressed in human breast carcinomas.

    abstract::The MLN64 gene, which is localized in q12-q21 of the human chromosome 17, encodes a novel protein containing 2 distinct domains. At the N-terminal, MLN64 exhibits a potential trans-membrane region, while at the C-terminal, it shares homology with the F26F4.4 protein of Coenorhabditis elegans and the steroidogenic acut...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Moog-Lutz C,Tomasetto C,Régnier CH,Wendling C,Lutz Y,Muller D,Chenard MP,Basset P,Rio MC

    更新日期:1997-04-10 00:00:00

  • Lymphoma development from transplanted murine leukemia virus infected organ cultured thymuses: inhibitory effect of in vitro interferon treatment.

    abstract::Thymuses of 14-day-old AKR mouse embryos were infected with Gross murine leukemia virus (MuLV) and then maintained in organ culture for 3 weeks. When they were transplanted to 3-week-old (AKR X C3H)F1 mice, approximately 50% of these developed T lymphomas within 3-4 months. Most (22/23) tumors were of host, F1-hybrid,...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Riesenfeld I,Tufveson G,Alm GV

    更新日期:1980-04-15 00:00:00

  • Clinicopathologic significance of urokinase receptor- and MMP-9-positive stromal cells in human colorectal cancer: functional multiplicity of matrix degradation on hematogenous metastasis.

    abstract::Our previous clinicopathologic study revealed an inverse association of liver metastasis of colorectal cancer and stromal expression of matrix metalloproteinase-9 (MMP-9) or urokinase receptor (uPAR). This suggests that host cells, particularly macrophages, expressing matrix-degrading enzymes/factors could be protecti...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Saito K,Takeha S,Shiba K,Matsuno S,Sorsa T,Nagura H,Ohtani H

    更新日期:2000-04-01 00:00:00

  • Association study of genetic variation in the autophagy lysosome pathway genes and risk of eight kinds of cancers.

    abstract::The autophagy lysosome pathway is essential to maintain cell viability and homeostasis in response to many stressful environments, which is reported to play a vital role in cancer development and therapy. However, the association of genetic alterations of this pathway with risk of cancer remains unclear. Based on geno...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Tan J,Fu L,Chen H,Guan J,Chen Y,Fang J

    更新日期:2018-07-01 00:00:00

  • Body size and composition and colon cancer risk in women.

    abstract::Studies of colon cancer risk in males have reported strong positive associations with obesity, particularly with central adiposity. The association has been weaker and less consistent for women. In a prospective cohort study of women, body measurements were taken directly; fat mass and fat-free mass being estimated by...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: MacInnis RJ,English DR,Hopper JL,Gertig DM,Haydon AM,Giles GG

    更新日期:2006-03-15 00:00:00

  • Breast cancer screening effect across breast density strata: A case-control study.

    abstract::Breast cancer screening is known to reduce breast cancer mortality. A high breast density may affect this reduction. We assessed the effect of screening on breast cancer mortality in women with dense and fatty breasts separately. Analyses were performed within the Nijmegen (Dutch) screening programme (1975-2008), whic...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: van der Waal D,Ripping TM,Verbeek AL,Broeders MJ

    更新日期:2017-01-01 00:00:00

  • The association between markers of tumour cell metabolism, the tumour microenvironment and outcomes in patients with colorectal cancer.

    abstract::Tumour cell anaerobic metabolism has been reported to be a prognostic factor in colorectal cancer. The present study investigated the association between monocarboxylate transporter (MCT) 1, MCT 2, lactate dehydrogenase (LDH) 1 and LDH 5, the tumour microenvironment, and outcome in patients with colorectal cancer. A c...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Roseweir AK,Clark J,McSorley ST,vanWyk HC,Quinn JA,Horgan PG,McMillan DC,Park JH,Edwards J

    更新日期:2019-05-01 00:00:00

  • Loss of hSef promotes metastasis through upregulation of EMT in prostate cancer.

    abstract::We have previously reported that the negative signaling regulator Similar Expression to FGF (hSef) is downregulated in prostate cancer and its loss is associated with clinical metastasis. Here, we explored the mechanistic basis of this finding. We first confirmed our clinical observation by testing hSef manipulation i...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hori S,Wadhwa K,Pisupati V,Zecchini V,Ramos-Montoya A,Warren AY,Neal DE,Gnanapragasam VJ

    更新日期:2017-04-15 00:00:00

  • MicroRNA alterations in head and neck squamous cell carcinoma.

    abstract::MicroRNAs (mirs) are small noncoding RNA molecules (~22 nucleotides) that regulate posttranscriptional gene expression. Currently, there has not been a comprehensive study of their role in primary head and neck squamous cell carcinoma (HNSCC). To determine the role of mirs in HNSCC, we screened for altered microRNA ex...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Chang SS,Jiang WW,Smith I,Poeta LM,Begum S,Glazer C,Shan S,Westra W,Sidransky D,Califano JA

    更新日期:2008-12-15 00:00:00

  • Epstein-Barr virus-related herpesvirus from a rhesus monkey (Macaca mulatta) with malignant lymphoma.

    abstract::A herpesvirus (RhEBV) was isolated from a lymphoblastoid cell line (LCL) that became established from a malignant lymphoma in a rhesus monkey. The predominant cell marker in the LCL was that of B lymphocytes. RhEBV-induced viral capsid (VCA) and nuclear antigens (NA) in the LCL were serologically related to similar an...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Rangan SR,Martin LN,Bozelka BE,Wang N,Gormus BJ

    更新日期:1986-09-15 00:00:00

  • The SCID mouse environment causes immunophenotypic changes in human immature T-cell lines.

    abstract::In order to evaluate whether the SCID mouse can provide the microenvironment for the growth of human immature T-cell leukemias and if in vivo growth alters their phenotype, we examined the behavior of 3 well-characterized T-cell acute lymphoblastic leukemia (T-ALL)-derived T-cell lines which are at different stages of...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Uittenbogaart CH,Anisman DJ,Tary-Lehmann M,Vollger LW,Breit TM,Van Dongen JJ,Saxon A

    更新日期:1994-02-15 00:00:00

  • Analgesics and cancers of the renal pelvis and ureter.

    abstract::To evaluate renal pelvis and ureter (RPU) cancer risk in relation to lifetime use of analgesics, a population-based case-control study was carried out in 3 areas of the United States. Among 502 cases and 496 controls diagnosed and interviewed during 1983-1986, no significant increases in risk were found for any of the...

    journal_title:International journal of cancer

    pub_type: 杂志文章,多中心研究


    authors: Linet MS,Chow WH,McLaughlin JK,Wacholder S,Yu MC,Schoenberg JB,Lynch C,Fraumeni JF Jr

    更新日期:1995-07-04 00:00:00

  • Trends in hysterectomy-corrected uterine cancer mortality rates during 2002 to 2015: mortality of nonendometrioid cancer on the rise?

    abstract::Corpus uteri cancer is the most common gynecological malignancy in most developed countries. The disease is typically diagnosed at an early stage, is of endometrioid histologic subtype, and has a fairly good prognosis. Here, we describe hysterectomy-corrected mortality rates of corpus uteri cancer, overall and stratif...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Gustafson LW,Booth BB,Kahlert J,Ørtoft G,Mejlgaard E,Clarke MA,Wentzensen N,Rositch AF,Hammer A

    更新日期:2021-02-01 00:00:00

  • Potentiation of the anti-tumor effect of actinomycin D by tumor necrosis factor alpha in mice: correlation between in vitro and in vivo results.

    abstract::The anti-tumor effects of actinomycin D (Act D) and recombinant human tumor necrosis factor (TNF)-alpha have been studied on 4 established murine tumor cell lines: MmB16 melanoma, Lewis lung (LL/2) carcinoma, L1 sarcoma and L1210 leukemia. During short-term incubation (24 hr) Act D produced dose-dependent cytostatic/c...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lasek W,Giermasz A,Kuc K,Wańkowicz A,Feleszko W,Gołab J,Zagozdzon R,Stokłosa T,Jakóbisiak M

    更新日期:1996-05-03 00:00:00

  • Impact of BCRP/MXR, MRP1 and MDR1/P-Glycoprotein on thermoresistant variants of atypical and classical multidrug resistant cancer cells.

    abstract::The impact of the ABC transporters breast cancer resistance protein/mitoxantrone resistance associated transporter (BCRP/MXR), multidrug resistance-associated protein 1 (MRP1) and multidrug resistance gene-1/P-glycoprotein (MDR1/PGP) on the multidrug resistance (MDR) phenotype in chemoresistance and thermoresistance w...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Stein U,Lage H,Jordan A,Walther W,Bates SE,Litman T,Hohenberger P,Dietel M

    更新日期:2002-02-20 00:00:00

  • Low expression of human tubulin tyrosine ligase and suppressed tubulin tyrosination/detyrosination cycle are associated with impaired neuronal differentiation in neuroblastomas with poor prognosis.

    abstract::Neuroblastoma (NBL), one of the most common childhood solid tumors, has a distinct nature in different prognostic subgroups. However, the precise mechanism underlying this phenomenon remains largely unknown. To understand the molecular and genetic bases of neuroblastoma, we have generated its cDNA libraries and identi...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Kato C,Miyazaki K,Nakagawa A,Ohira M,Nakamura Y,Ozaki T,Imai T,Nakagawara A

    更新日期:2004-11-10 00:00:00

  • Idiotypic protein-pulsed dendritic cell vaccination in multiple myeloma.

    abstract::Idiotypic protein (Id) produced by myeloma cells is clone-specific and may be a suitable tumor-specific antigen for immune targeting. Advances in dendritic cell (DC) technology suggest the opportunity for using this potent antigen presentation system to deliver myeloma Id to the autologous host to elicit anti-tumor im...

    journal_title:International journal of cancer

    pub_type: 临床试验,杂志文章


    authors: Lim SH,Bailey-Wood R

    更新日期:1999-10-08 00:00:00