Abstract:
:Primary mediastinal B-cell lymphoma is a locally highly aggressive but poorly disseminating tumor composed of medium sized or large cells most probably of thymic medullary origin. It has a mature B-cell phenotype, typically lacks immunoglobulin expression and has variable defects in expression of HLA-molecules. We present here a cell line, MedB-1, derived from such a tumor. As is frequently found in mediastinal B-cell lymphomas in situ, MedB-1 is CD10(-), CD19(+), CD21(-), CD22(+), CD23(+), CD25(-), CD37(+), CD38(-), CD39(+), CD40(+), CD54(+), CD95(+). Like the parental tumor, MedB-1 lacks HLA-A,B,C alpha-chains and beta(2)microglobulin and expresses HLA-D molecules at decreased levels. Both parental tumor and MedB-1 cells are clonally related as shown by immunoglobulin heavy chain gene rearrangement analysis. Unlike the parental tumor tissue, the MedB-1 cell line cytoplasmically expresses IgG/kappa in a very small subset of cells under standard culture conditions. MedB-1 does not contain any Epstein-Barr virus DNA. In a tissue adhesion assay MedB-1 cells showed an extensive binding to the medullary region of normal thymus. Altogether, MedB-1 is a suitable tool for functional and molecular analysis of this distinct lymphoma entity.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Möller P,Brüderlein S,Sträter J,Leithäuser F,Hasel C,Bataille F,Moldenhauer G,Pawlita M,Barth TFdoi
10.1002/ijc.1211keywords:
subject
Has Abstractpub_date
2001-05-01 00:00:00pages
348-53issue
3eissn
0020-7136issn
1097-0215pii
10.1002/ijc.1211journal_volume
92pub_type
杂志文章abstract::The partition-defective 3 (PAR-3) protein is implicated in the development and maintenance of cell polarity and is associated with proteins that mediate the changes in cytoskeleton organization required for cell polarity establishment. In this work, we used two original primary cell lines (R-180 and R-305) derived fro...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28548
更新日期:2014-05-01 00:00:00
abstract::Immunochromatographical fecal occult blood tests were shown to have higher sensitivity for detecting colorectal neoplasms than the commonly used guaiac-based test. However, positivity rates, sensitivity and specificity vary widely. We aimed to assess the reasons for this heterogeneity. Six dichotomous (qualitative) im...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25154
更新日期:2010-10-01 00:00:00
abstract::Epidemiological studies of in situ breast cancer are sparse, and the role of reproductive history, an established risk modifier for invasive breast cancer, remains incompletely investigated. To examine possible associations with parity and age at first birth, we undertook a case-control study nested in a nationwide co...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980729)77:3<330::aid-ijc
更新日期:1998-07-29 00:00:00
abstract::To identify molecular markers for the progression of head-and-neck squamous cell carcinoma (HNSCC), we used RNA arbitrarily primed (RAP) PCR to determine the qualitative and quantitative differences in gene expression between normal epithelial cells, those derived from dysplastic oral mucosa and invasive and metastati...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10403
更新日期:2002-06-01 00:00:00
abstract::A hospital-based case-control study of renal-cell carcinoma was conducted in France from 1987 to 1991. A total of 196 histologically confirmed cases (138 males and 58 females) and 347 controls (235 males and 112 females), matched for sex, age at interview, hospital and interviewer, were included. The risk of renal-cel...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910550107
更新日期:1993-08-19 00:00:00
abstract::Oncogenic activation of MYC occurs often in breast carcinoma and is associated with poor prognosis. Loss or inactivation of mechanisms that restrain MYC may therefore be involved in tumor progression. In this study, we show that the MYC-interacting adaptor protein BIN1 is frequently missing in malignant breast cells a...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::Specific estrogen, progesterone and androgen receptors were determined in 19 well-characterized squamous-cell carcinoma (SCC) cell lines. Ten of the lines were derived from patients with SCC of the larynx and nine were from patients with SCC originating in other areas of the head and neck. Estrogen receptors (ER) were...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910330105
更新日期:1984-01-15 00:00:00
abstract::Bromodomain and extraterminal domain (BET) inhibitors are broadly active against distinct types of cancer, including nonsmall cell lung cancer (NSCLC). Previous studies have addressed the effect of BET-inhibiting drugs on the expression of oncogenes such as c-Myc, but DNA damage repair pathways have also been reported...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32515
更新日期:2020-02-15 00:00:00
abstract::Gastric cancer is the second most common cause of cancer deaths worldwide. The identification of molecular genetic parameters that are associated with response to chemotherapy and prognosis is of utmost interest. We examined methylation of the apoptosis-related genes, TMS1 and DAPK, in 81 primary gastric cancers using...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23143
更新日期:2008-02-01 00:00:00
abstract::Urinary bladder cancer is a heterogeneous disease with tumors ranging from papillary noninvasive (stage Ta) to solid muscle infiltrating tumors (stage T2+). The risk of progression and death for the most frequent diagnosed type, Ta, is low, but the high incidence of recurrences has a significant effect on the patients...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24954
更新日期:2010-03-15 00:00:00
abstract::Neuron-specific src mRNA, which is expressed in human brain tissue by alternative splicing, is associated with neural differentiation. Neuronal c-srcNI expression may be associated with the ability of neuroblastomas to mature; furthermore, c-srcN2 mRNA is induced in chemically differentiated neuroblastoma cells in vit...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910580607
更新日期:1994-09-15 00:00:00
abstract::Alterations of gene expression in squamous cell carcinoma (SCC) cell lines derived from the larynx and keratinocytes derived from adjacent normal mucosa of the larynx have been studied using the mRNA differential display technique. Lane-to-lane comparison of reverse transcribed mRNA showed a strong repression of a 148...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19991210)83:6<750::aid-ijc
更新日期:1999-12-10 00:00:00
abstract::Southern blot analyses of surgical specimens of cervical carcinoma from Japanese patients showed that 3/9 samples contained human papillomavirus (HPV) type-16 DNA sequences, and 2 contained HPV type-18 DNA sequences. By Northern blot analyses, RNA transcripts of HPV DNA sequences were demonstrated in some of the tissu...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910370405
更新日期:1986-04-15 00:00:00
abstract::The mismatch repair (MMR) genes are involved in the maintenance of genomic integrity. Recently, we showed that common variants in these genes are unlikely to contribute significantly to colorectal cancer risk. The aim of this study was to investigate the role of common variants in the mismatch repair pathway as progno...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24120
更新日期:2009-04-15 00:00:00
abstract::Children are especially sensitive to ionizing radiation and chemical carcinogens, and limiting their cancer risk is of great public concern. Calorie restriction (CR) is a potent intervention for suppressing cancer. However, CR is generally not appropriate for children. This study, therefore, examined to see if adult-o...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28751
更新日期:2014-09-01 00:00:00
abstract::No animal model exists for the in vivo growth of Hodgkin's-lymphoma-derived cells. Neither unmanipulated Hodgkin's-disease(HD)-derived cell lines nor primary biopsy tissue could be grown in nude mice. Since the severe combined immunodeficient (SCID) mouse has been reported to be a better recipient for transplanted hum...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910520610
更新日期:1992-12-02 00:00:00
abstract::Human non-small cell lung cancer (NSCLC) cells were transfected with recombinant prodrug herpes simplex virus type I thymidine kinase (HSV-tk) cDNA, and the selected clones underwent apoptosis in response to induction by antiviral ganciclovir (GCV). The efficiency of GCV-induced growth inhibition and the extent of the...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10701
更新日期:2002-12-01 00:00:00
abstract::Obesity has been postulated to increase the risk of colorectal cancer by mechanisms involving insulin resistance and the metabolic syndrome. We examined the associations of body mass index (BMI), waist circumference, the metabolic syndrome, metabolic obesity phenotypes and homeostasis model-insulin resistance (HOMA-IR...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.31345
更新日期:2018-08-01 00:00:00
abstract::In 1927 Otto Warburg established that tumours derive energy primarily from the conversion of glucose to lactic acid and only partially through cellular respiration involving oxygen. In the 1950s he proposed that all causes of cancer reflected different mechanisms of disabling cellular respiration in favour of fermenta...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.29012
更新日期:2015-10-01 00:00:00
abstract::A panel of 7 diagnostic cytopathologists, i.e., physicians trained to diagnose the malignant potential of human cells in Papanicolaou-stained smears, was asked to evaluate two sets of microscope slides of stained coverslip preparations of 28 cell culture lines, 15 of which were neoplastic. Slide Set I consisted of 13 ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910380310
更新日期:1986-09-15 00:00:00
abstract::The incidence of non-Hodgkin's lymphoma (NHL) has markedly increased in the US and other westernized countries in recent years and presents a considerable clinical challenge. NHL is divided into subtypes that follow an aggressive or indolent course. Follicular lymphoma (FL), the most common indolent subtype, and mantl...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.21805
更新日期:2006-09-01 00:00:00
abstract::The Insulin-like growth factor (IGF)/IGF-receptor pathway with its scaffolding proteins Insulin Receptor Substrate (IRS)1 and IRS2 are crucial regulators of metabolism and progression in metastatic colorectal cancer (mCRC). The goal of the study was the identification of predictive and prognostic markers among IRS1, I...
journal_title:International journal of cancer
pub_type: 杂志文章,随机对照试验
doi:10.1002/ijc.30715
更新日期:2017-07-15 00:00:00
abstract::Loss of cell polarity is one of the hallmarks of malignant carcinomas. Most of the understanding about the link between cell polarity and proliferation control comes from studies on the Drosophila tumor suppressors discs large (Dlg), scribble (Scrib) and lethal giant larvae (lgl). Mammalian homologues of these protein...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21982
更新日期:2006-09-15 00:00:00
abstract::Our previous clinicopathologic study revealed an inverse association of liver metastasis of colorectal cancer and stromal expression of matrix metalloproteinase-9 (MMP-9) or urokinase receptor (uPAR). This suggests that host cells, particularly macrophages, expressing matrix-degrading enzymes/factors could be protecti...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(20000401)86:1<24::aid-ijc4
更新日期:2000-04-01 00:00:00
abstract::Clinical features, 8 histological variables, 7 nuclear morphometric variables, 2 mitotic indices, oestrogen-receptor (ER) and progesterone-receptor content (PR), DNA ploidy and S-phase fraction (SPF) were entered in a Cox's model to assess their independent predictive value in 216 breast-cancer patients followed up fo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910510409
更新日期:1992-06-19 00:00:00
abstract::Annamycin (Ann) is a non-cross-resistant lipophilic anthracycline antiobiotic optimally suited for liposome delivery. We studied how vesicle size and presence of phospholipids with a high phase transition temperature and monosialoganglioside (GM I) in the liposome bilayers affect the pharmacokinetics, tumor selectivit...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910610513
更新日期:1995-05-29 00:00:00
abstract::We have studied the in vitro invasive properties of 3 cell lines derived from the co-transfection of rat embryo fibroblasts (REF) with EIA genes deficient in exon 2 and T24-ras. All 3 cell lines showed invasive properties at passage 10 after isolation. Invasive cells expressed elevated levels of stromelysin-1 and redu...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910510516
更新日期:1992-07-09 00:00:00
abstract::We previously determined that human papillomavirus (HPV) virus-like particles (VLPs) and pseudovirions (PsV) did not, respectively, bind to or infect intact epithelium of the cervicovaginal tract. However, they strongly bound heparan sulfate proteoglycans (HSPG) on the basement membrane of disrupted epithelium and inf...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29823
更新日期:2016-02-15 00:00:00
abstract::CD16 surface antigens represent activatory molecules in CD3-16+ NK cells. In order to target NK cells against relatively NK-resistant ovarian carcinomas, we used an anti-CD16 monoclonal antibody (MAb) (VD4), together with an anti-ovarian carcinoma-associated antigen (MOV19), to construct biMAbs. To this end, hybrid hy...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910480213
更新日期:1991-05-10 00:00:00
abstract::Survivin protein is an attractive candidate for cancer immunotherapy since it is abundantly expressed in most common human cancers and mostly absent in normal adult tissues. Malignant mesothelioma (MM) is a deadly cancer associated with asbestos or erionite exposure for which no successful therapies are currently avai...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28048
更新日期:2013-08-01 00:00:00