Glycan composition of follicle (Sertoli) cells of the amphibian Pleurodeles waltl. A lectin histochemical study.

Abstract:

:The glycan composition of the N- and O-linked oligosaccharides of the follicle (Sertoli) cells of the urodele amphibian Pleurodeles waltl testis were identified by lectin histochemistry, performed alone or in combination with enzymatic and chemical deglycosylation methods. The follicle cells were shown to contain: (1) Fuc, Galbeta(1,4)GlcNAc, GalNAc and Neu5Acalpha(2,3)Galbeta(1,4)GlcNAc in both N- and O-linked oligosaccharides; (2) Man in N-linked glycans; and (3) Galbeta(1,3)GalNAc in O-linked sugar chains. The follicle cells at the pre- and postmeiotic stages showed some differences in the UEA-1-positive Fuc characterisation, suggesting differences in the glycan composition. In addition, the sequence Neu5Acalpha(2,6)Gal/GalNAc was shown in the follicle cells only after spermiation, in the sperm-empty lobules of the developing glandular tissue. These results suggest that the follicle cells modify their glycoprotein content, probably for the performance of new roles, as the spermatogenetic cells develop. Thus the follicle cells surrounding male germ cells at different spermatogenetic stages would contain different glycoproteins involved in specific roles during male germ cell proliferation and maturation.

journal_name

J Anat

journal_title

Journal of anatomy

authors

Sáez FJ,Madrid JF,Alonso E,Hernández F

doi

10.1046/j.1469-7580.2001.19860673.x

keywords:

subject

Has Abstract

pub_date

2001-06-01 00:00:00

pages

673-81

issue

Pt 6

eissn

0021-8782

issn

1469-7580

journal_volume

198

pub_type

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