Abstract：
:In stem-cell research a major difficulty is caused by the lack of distinctive features that allow the identification of human mesenchymal stem cells (hMSC). Until now, there has been no specific marker and the most common way to identify hMSC is by their characteristic stem-cell properties: self-replication and differentiation potential. However, these findings can only be revealed retrospectively, and, once differentiated, hMSC lose their stem-cell character. The aim of this study was to establish four-colour immunofluorescence of several markers simultaneously in order to address the problem of how to identify hMSC on the single-cell level. The four markers collagen-I, collagen-IV, fibronectin and CD44 are known to be expressed by hMSC. Antibody binding was detected using secondary antibodies conjugated to FITC, Alexa546, TexasRed and AMCA. Because the distinction between Alexa546 and TexasRed was not possible on conventional digital images using standard filter sets, we performed spectral image acquisition. The image was subsequently decomposed into its pure spectral components, which permitted linear unmixing. Using this procedure we were able to demonstrate four-colour immunofluorescence on hMSC. With the possibility of using more sophisticated marker profiles and/or additional markers, four-colour immunofluorescence offers the opportunity of identifying hMSC on the single-cell level without performing differentiation assays.
journal_name
J Anatjournal_title
Journal of anatomyauthors
Schieker M,Pautke C,Reitz K,Hemraj I,Neth P,Mutschler W,Milz Sdoi
10.1111/j.1469-7580.2004.00252.xkeywords:
subject
Has Abstractpub_date
2004-02-01 00:00:00pages
133-9issue
2eissn
0021-8782issn
1469-7580pii
JOA252journal_volume
204pub_type
杂志文章abstract：:Kinetic changes have been studied in the transitional cells of the marrow in normal, hypoxic (0.5 atm) and rebound rats. Suspensions of marrow cells were incubated with tritiated thymidine for 1 hour, and the percentage of labelled transitionals of all sizes, as well as of large transitionals, was couinted in radioaut...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：:The study of human anatomy can be found throughout the rich history of Persia. For thousands of years, morphological descriptions derived from this part of the world have contributed to and have helped form our current anatomical knowledge base. In this article we review the major influential Persian periods and the i...
journal_title：Journal of anatomy
pub_type： 历史文章,杂志文章
doi：10.1111/j.1469-7580.2007.00711.x
更新日期：2007-04-01 00:00:00
abstract：:The neuromuscular junction (NMJ) is the highly specialised peripheral synapse formed between lower motor neuron terminals and muscle fibres. Post-synaptic acetylcholine receptors (AChRs), which are found in high density in the muscle membrane, bind to acetylcholine released into the synaptic cleft of the NMJ, thereby ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.13228
更新日期：2020-10-01 00:00:00
abstract：:It is a challenge to understand how barn owls (Tyto alba) reduce noise during flight to be able to hunt small mammals by audition. Several specializations of the wing and the wing feathers have been implicated in noise reduction. What has been overlooked so far are the fringes at the inner vanes of remiges. We demonst...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2012.01504.x
更新日期：2012-07-01 00:00:00
abstract：:We studied the cerebellar connections to the reticular nucleus thalamus (RNT) by means of retrograde axonal transport of horseradish peroxidase (HRP) in the rat. Specific HRP pressure injections to the rostral RNT (1.6-1.8 mm caudal to bregma) resulted in retrograde labelling of neurones in the cerebellar nuclei. The ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.2002.00119.x
更新日期：2002-12-01 00:00:00
abstract：:Surgical interruption of the gastrointestinal tract is widely used for investigating the structure of the enteric nervous system and in the treatment of certain pathological conditions of the gastrointestinal tract. The effects of transection and end-to-end anastomosis and myotomy on nerve cells of the myenteric plexu...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:Amniotic fluid-derived stem cells (AFSCs) are a unique stem cell source that may have great potential for use in tissue engineering (TE) due to their pluripotentiality. AFSCs have previously shown angiogenic potential and may present an alternative cell source for endothelial-like cells that could be used in range of ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12283
更新日期：2015-12-01 00:00:00
abstract：:The structure of amniotic papillae in sheep was investigated by light transmission and scanning electron microscopy. Papillae were found on the amnion near the umbilical cord in a majority of the sheep examined, from early mid-term onwards. The papillae possessed a basic connective tissue core with a varying degree of...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1978-06-01 00:00:00
abstract：:The calcaneocuboid joint was studied in ligamentous specimens of ten human feet, and in skeletons of two gorillas (Gorilla gorilla beringei), six chimpanzees (Pan troglodytes), three orangutans (Pongo pygmaeus) and 25 human feet. The movement of the transverse tarsal joint was further studied in a living foot on a wal...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1979-08-01 00:00:00
abstract：:Homoplastic grafts of re-orientated unsegmented paraxial mesoderm transplanted from stage 20 Xenopus embryos into host embryos of the same age resulted in segmentation and the formation of somites in the same axis as if they had been left in situ. Because grafts transplanted with various orientations came under the st...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1983-06-01 00:00:00
abstract：:The natural radiographic appearance of the various bones of the skeleton are described for several strains of laboratory mice. The Harwell substrains of CBA, A and 101 are generally similar and become osteoporotic on ageing. Harwell C57BL have similar, but more delicately chiseled, bones. Harwell C3H mice have bones w...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：:The regeneration of adrenocortical autotransplants in the rat spleen has been investigated by light and electron microscopy. Up to the seventh day after implantation, adrenal grafts showed large areas of necrosis and contained many degenerating (apoptotic) adrenocortical cells, some mesenchyme-like poorly differentiat...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1982-09-01 00:00:00
abstract：:The ultrastructure of 3 fibrocartilages is described at the insertion of the adult rat Achilles tendon. Enthesial fibrocartilage lies at the tendon-bone junction, sesamoid fibrocartilage in the deep surface of the tendon where it presses on the calcaneus, and periosteal fibrocartilage covers the opposing surface of th...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:Male rats undernourished from about birth until 100 days of age were nutritionally rehabilitated until 200 days. Six control and six experimental rats were anaesthetised and X-rayed at each of 50, 100 and 200 days. Measurements of various skeletal dimensions, and estimates of skeletal maturity, were made from these ra...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1985-08-01 00:00:00
abstract：:Current surgical treatments for degenerative intervertebral disc disease do not restore full normal spinal movement. Tissue engineering a functional disc replacement may be one way to circumvent this limitation, but will require an integration of the different tissues making up the disc for this approach to be success...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2012.01537.x
更新日期：2012-12-01 00:00:00
abstract：:Vascular sympathetic neuroeffector associations have been examined in rat iris arterioles using serial section electron microscopy and reconstruction techniques. Examination of random sections showed that, of all profiles of varicosities (199) seen to lie closer than 4 microns to vascular smooth muscle cells, only a s...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.1998.19210045.x
更新日期：1998-01-01 00:00:00
abstract：:The paraspinal compartment acts as a bone-muscle composite beam of the spine. The elastic properties of the paraspinal muscles play a critical role in spine stabilization. These properties depend on the subjects' posture, and they may be drastically altered by low back pain. Supersonic shear wave elastography can be u...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12978
更新日期：2019-06-01 00:00:00
abstract：:A vomeronasal organ and patent nasopalatine ducts were found in adult specimens of Aotus trivirgatus, Arctocebus calabarensis, Ateles geoffroyi and Saguinus fuscicollis. The vomeronasal complex appeared to be functional except in Ateles. However, the relative size of the organ in the Platyrrhini is much reduced compar...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
abstract：:A one day symposium of the Anatomical Society of Great Britain and Ireland was held at the Royal Free Hospital in London on the 17th September 1999, on the topic of 'Mechanisms of ageing and longevity'. The following are abstracts of communications and posters presented at the meeting. ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.2000.19620293.x
更新日期：2000-02-01 00:00:00
abstract：:Adult male Sprague-Dawley rats were randomly assigned to two groups: control and anaemic. Anaemia was induced by periodical blood withdrawal. Extensor digitorum longus and soleus muscles were excised under pentobarbital sodium total anaesthesia and processed for transmission electron microscopy, histochemical and bioc...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2008.00908.x
更新日期：2008-06-01 00:00:00
abstract：:The differentiation of the bulbar region and arterial outflow tract of the developing mouse heart was investigated by analysing serial transverse sections through the heart region of mouse embryos isolated between the eleventh and fifteenth day of gestation. Over this period of time, we observed the configurational an...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:A symposium on extramedullary haematopoiesis was held on 11-12 September 1996 at the University of St Andrews. The following are abstracts of papers and posters presented at the meeting. ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.1997.19020311.x
更新日期：1997-02-01 00:00:00
abstract：:The ventricular surface features of the pineal recess of 35 adult brush-tailed possums (Trichosurus vulpecula) were studied, mainly with the scanning microscope. The complex and interesting details observed have not been reported before. The pineal recess shows three distinct zones, and it is suggested that these be c...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1979-10-01 00:00:00
abstract：:The histochemical and ultrastructural characteristics of the adult rat and rabbit costal diaphragm were investigated. On the basis of enzyme histochemistry, the rat diaphragm was found to contain 42% and 39% Type I, 24% and 25% Type II A and 33% and 34% Type II B fibres on the thoracic and abdominal surfaces respectiv...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1990-02-01 00:00:00
abstract：:This investigation is based upon the pineal organs of 92 specimens of 36 species of the family Pteropodidae (Mammalia, Chiroptera). The size of the megachiropteran pineal correlates well with body size (r = 0.864), confirming the former conclusions that generally larger bodied bats have larger pineals. The range of th...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1990-02-01 00:00:00
abstract：:In order to investigate further the effects of paralysis on skeletal development in the chick embryo, paralysis was induced at 6 days of incubation by dropping 0.2% solution of decamethonium bromide onto the chorioallantoic membrane and maintaining paralysis through to 20 days of incubation. General effects of paralys...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1991-08-01 00:00:00
abstract：:Two endocrine cell types were found in the metaplastic epithelium of gall bladders removed for gall stones. The endocrine cell type I resembled the EC (enterochromaffin) cell of the normal stomach mucosa. The homogeneous and electron-dense secretory granules were variable in size and shape and mainly located in the su...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1975-11-01 00:00:00
abstract：:The presence of nerve fibres in the medulla of the chick thymus has been investigated ultrastructurally. Unmyelinated nerve fibres were found not only in the perivascular space but also among the myoid cells. The nerve fibres were situated in close proximity to the myoid cells. The unmyelinated axons, containing predo...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:Apoptosis is an essential physiological process in embryonic development. In the developing eye of vertebrates, three periods of developmental apoptosis can be distinguished: early, intermediate and later. Within the apoptosis pathway, caspases play a crucial role. It has also been shown that HSP110 may have a potenti...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2007.00719.x
更新日期：2007-05-01 00:00:00
abstract：:A defining feature of dentitions in modern sharks and rays is the regulated pattern order that generates multiple replacement teeth. These are arranged in labio-lingual files of replacement teeth that form in sequential time order both along the jaw and within successively initiated teeth in a deep dental lamina. Two ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12796
更新日期：2018-06-01 00:00:00
