Abstract:
:We define here the specificity and significance of proteases in the endoplasmic reticulum (ER) that generate peptides for presentation by major histocompatibility complex (MHC) class I molecules. We show that aminopeptidases efficiently trimmed all residues except proline that flank the NH2-termini of antigenic precursors in the ER and caused an accumulation of X-P-Xn peptides. An aminopeptidase inhibitor blocked peptide trimming in the ER and, consequently, the generation of peptide-loaded MHC molecules. Peptide trimming in the ER is therefore a key step in the MHC class I antigen-processing pathway and also explains the paradox of why many MHC class I molecules display peptides with the X-P-Xn motif despite the inability of the transporter associated with antigen processing to transport such peptides from the cytoplasm.
journal_name
Nat Immunoljournal_title
Nature immunologyauthors
Serwold T,Gaw S,Shastri Ndoi
10.1038/89800keywords:
subject
Has Abstractpub_date
2001-07-01 00:00:00pages
644-51issue
7eissn
1529-2908issn
1529-2916pii
89800journal_volume
2pub_type
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