Abstract:
:RecBCD enzyme is a processive DNA helicase and nuclease that participates in the repair of chromosomal DNA through homologous recombination. We have visualized directly the movement of individual RecBCD enzymes on single molecules of double-stranded DNA (dsDNA). Detection involves the optical trapping of solitary, fluorescently tagged dsDNA molecules that are attached to polystyrene beads, and their visualization by fluorescence microscopy. Both helicase translocation and DNA unwinding are monitored by the displacement of fluorescent dye from the DNA by the enzyme. Here we show that unwinding is both continuous and processive, occurring at a maximum rate of 972 +/- 172 base pairs per second (0.30 microm s(-1)), with as many as 42,300 base pairs of dsDNA unwound by a single RecBCD enzyme molecule. The mean behaviour of the individual RecBCD enzyme molecules corresponds to that observed in bulk solution.
journal_name
Naturejournal_title
Natureauthors
Bianco PR,Brewer LR,Corzett M,Balhorn R,Yeh Y,Kowalczykowski SC,Baskin RJdoi
10.1038/35053131keywords:
subject
Has Abstractpub_date
2001-01-18 00:00:00pages
374-8issue
6818eissn
0028-0836issn
1476-4687journal_volume
409pub_type
杂志文章相关文献
NATURE文献大全abstract::How long does it take a random walker to reach a given target point? This quantity, known as a first-passage time (FPT), has led to a growing number of theoretical investigations over the past decade. The importance of FPTs originates from the crucial role played by first encounter properties in various real situation...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature06201
更新日期:2007-11-01 00:00:00
abstract::Villin is an actin-binding protein of relative molecular mass (Mr) 95,000 found in the core bundle of microfilaments in brush border microvilli from intestine. In physiological calcium concentrations (less than 1 microM), villin crosslinks actin filaments into bundles. However, in free calcium concentrations (greater ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/315248a0
更新日期:1985-05-16 00:00:00
abstract::Q beta replicase, in the absence of added template, will synthesize RNA autocatalytically. A variety of small RNa species, termed '6S RNAs' are generated. As this reaction purportedly occurs in the absence of template, it has been termed 'de novo' RNA synthesis. The question of whether Q beta replicase can polymerize ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/301350a0
更新日期:1983-01-27 00:00:00
abstract::Cell-free DNA in the blood provides a non-invasive diagnostic avenue for patients with cancer1. However, characteristics of the origins and molecular features of cell-free DNA are poorly understood. Here we developed an approach to evaluate fragmentation patterns of cell-free DNA across the genome, and found that prof...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-019-1272-6
更新日期:2019-06-01 00:00:00
abstract::Tau is one of the diverse group of microtubule-associated proteins that bind to microtubules and may thereby influence their structure and function. It occurs in the mammalian brain, mainly in axons, and is a component of the neurofibrillary tangles of Alzheimer's disease. Tau was recently sequenced, but there remains...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/334359a0
更新日期:1988-07-28 00:00:00
abstract::The exceptionally broad species diversity of vascular plant genera in east Asian temperate forests, compared with their sister taxa in North America, has been attributed to the greater climatic diversity of east Asia, combined with opportunities for allopatric speciation afforded by repeated fragmentation and coalesce...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35093166
更新日期:2001-09-13 00:00:00
abstract::Wolbachia is a common maternally inherited bacterial symbiont able to induce crossing sterilities known as cytoplasmic incompatibility (CI) in insects. Wolbachia-modified sperm are unable to complete fertilization of uninfected ova, but a rescue function allows infected eggs to develop normally. By providing a reprodu...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature03629
更新日期:2005-07-14 00:00:00
abstract::The emission of isoprene from the leaves of forest trees is a fundamental component of biosphere-atmosphere interactions, controlling many aspects of photochemistry in the lower atmosphere. As almost all commercial agriforest species emit high levels of isoprene, proliferation of agriforest plantations has significant...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature01312
更新日期:2003-01-16 00:00:00
abstract::Cyclosporin A, a cyclic undecapeptide, is a potent immunosuppressant that binds to a peptidyl-prolyl cis-trans isomerase of 165 amino acids, cyclophilin. The cyclosporin A/cyclophilin complex inhibits the calcium- and calmodulin-dependent phosphatase, calcineurin, resulting in a failure to activate genes encoding inte...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/361088a0
更新日期:1993-01-07 00:00:00
abstract::In addition to coordinating immune and inflammatory responses, NF-kappaB/Rel transcription factors control cell survival. Normally, NF-kappaB dimers are sequestered in the cytoplasm by binding to inhibitory IkappaB proteins, and can be activated rapidly by signals that induce the sequential phosphorylation and proteol...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35104560
更新日期:2001-11-15 00:00:00
abstract::The respiratory organs of terrestrial insects consist of tracheal tubes with external spiracular valves that control gas exchange. Despite their relatively high metabolic rate, many insects have highly discontinuous patterns of gas exchange, including long periods when the spiracles are fully closed. Two explanations ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature03106
更新日期:2005-02-03 00:00:00
abstract::The retina in most mammals contains two types of macroglial cells--Müller cells, which span the entire thickness of the retina, and astrocytes, which are mainly confined to the nerve fibre layer. Whereas Müller cells are diffusely distributed in all vertebrate retinae, the presence and distribution of retinal astrocyt...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/332834a0
更新日期:1988-04-28 00:00:00
abstract::Antigen recognition by T lymphocytes is mediated by cell-surface glycoproteins known as T-cell antigen receptors (TCRs). These are composed of alpha and beta, or gamma and delta, polypeptide chains with variable (V) and constant (C) regions. In contrast to alphabeta TCRs, which recognize antigen only as peptide fragme...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35172
更新日期:1998-01-29 00:00:00
abstract::Graphene oxide membranes-partially oxidized, stacked sheets of graphene-can provide ultrathin, high-flux and energy-efficient membranes for precise ionic and molecular sieving in aqueous solution. These materials have shown potential in a variety of applications, including water desalination and purification, gas and ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature24044
更新日期:2017-10-19 00:00:00
abstract::Arachidonic acid is metabolised either by cyclooxygenases to produce prostaglandins and thromboxanes or by lipoxygenases to produce mono-, di- and trihydroxyeicosatetraenoic acids (HETEs). Polymorphonuclear leukocytes (PMNs) release HETEs, including mono- and dihydroxy fatty acids, when exposed to stimuli such as the ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/286264a0
更新日期:1980-07-17 00:00:00
abstract::Water-driven fracture propagation beneath supraglacial lakes rapidly transports large volumes of surface meltwater to the base of the Greenland Ice Sheet. These drainage events drive transient ice-sheet acceleration and establish conduits for additional surface-to-bed meltwater transport for the remainder of the melt ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature14480
更新日期:2015-06-04 00:00:00
abstract::Genetic testing will become the future standard of medical care. Life insurers will also need access to genetic information if the insurance industry is to survive intact and if cover is to remain affordable. ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/376013a0
更新日期:1995-07-06 00:00:00
abstract::Pathfinding by growing axons in the developing nervous system may be guided by gradients of extracellular guidance factors. Analogous to the process of chemotaxis in microorganisms, we found that axonal growth cones of cultured Xenopus spinal neurons exhibit adaptation during chemotactic migration, undergoing consecut...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature745
更新日期:2002-05-23 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a degenerative disorder of motor neurons in the cortex, brainstem and spinal cord. Its cause is unknown and it is uniformly fatal, typically within five years. About 10% of cases are inherited as an autosomal dominant trait, with high penetrance after the sixth decade. In most in...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/362059a0
更新日期:1993-03-04 00:00:00
abstract::The ras-related low-molecular-mass GTPases participate in signal transduction involving a variety of cellular functions, including cell-cycle progression, cellular differentiation, cytoskeletal organization, protein transport and secretion. The cycling of these proteins between GTP-bound and GDP-bound states is partia...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/359153a0
更新日期:1992-09-10 00:00:00
abstract::Injury causes a systemic inflammatory response syndrome (SIRS) that is clinically much like sepsis. Microbial pathogen-associated molecular patterns (PAMPs) activate innate immunocytes through pattern recognition receptors. Similarly, cellular injury can release endogenous 'damage'-associated molecular patterns (DAMPs...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature08780
更新日期:2010-03-04 00:00:00
abstract::Diatoms are one of the most ecologically successful classes of photosynthetic marine eukaryotes in the contemporary oceans. Over the past 30 million years, they have helped to moderate Earth's climate by absorbing carbon dioxide from the atmosphere, sequestering it via the biological carbon pump and ultimately burying...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature14599
更新日期:2015-08-20 00:00:00
abstract::Since Bridgman's discovery of five solid water (H2O) ice phases1 in 1912, studies on the extraordinary polymorphism of H2O have documented more than seventeen crystalline and several amorphous ice structures2,3, as well as rich metastability and kinetic effects4,5. This unique behaviour is due in part to the geometric...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-019-1114-6
更新日期:2019-05-01 00:00:00
abstract::Microtubules have a central role in eukaryotic cell polarity, in part through interactions between microtubule end-binding proteins and the cell cortex. In the fission yeast Schizosaccharomyces pombe, microtubules and the polarity modulator tea1p maintain cylindrical cell shape and strictly antipodal cell growth. The ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature01672
更新日期:2003-06-05 00:00:00
abstract::Susceptibility of rodent cells to infection by ecotropic murine leukaemia viruses (MuLV) is determined by binding of the virus envelope to a membrane receptor that has multiple membrane-spanning domains. Cells infected by ecotropic MuLV synthesize envelope protein, gp70, which binds to this receptor, thereby preventin...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/352725a0
更新日期:1991-08-22 00:00:00
abstract::Although the nuclear properties of the late actinides (plutonium, americium and curium) are fully understood and widely applied to energy generation, their solid-state properties do not fit within standard models and are the subject of active research. Plutonium displays phases with enormous volume differences, and bo...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature05647
更新日期:2007-03-29 00:00:00
abstract::How does the human visual system 'bind' different fragments in the visual scene to create enduring representations of objects? A visual illusion known as 'metacontrast' or backward masking provides compelling evidence that perception is not instantaneous and that it occurs sequentially in distinct stages. If a solid w...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/373066a0
更新日期:1995-01-05 00:00:00
abstract::The size of plants is largely determined by growth of the stem. Stem elongation is stimulated by gibberellic acid1-3. Here we show that internode stem elongation in rice is regulated antagonistically by an 'accelerator' and a 'decelerator' in concert with gibberellic acid. Expression of a gene we name ACCELERATOR OF I...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-2501-8
更新日期:2020-08-01 00:00:00
abstract::Sensory neuroscience seeks to understand how the brain encodes natural environments. However, neural coding has largely been studied using simplified stimuli. In order to assess whether the brain's coding strategy depends on the stimulus ensemble, we apply a new information-theoretic method that allows unbiased calcul...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature04519
更新日期:2006-02-23 00:00:00
abstract::The bmi-1 proto-oncogene can be activated by Moloney murine leukaemia proviral insertions in E mu-myc transgenic mice. It encodes a highly conserved nuclear protein of 324 amino acids which belongs to a family of proteins containing a putative new zinc-finger. Another closely related member of this family is the mouse...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/353353a0
更新日期:1991-09-26 00:00:00