Abstract:
:We have used differential-display PCR (DD-PCR) to compare renal-cell carcinoma (RCC) and normal kidney gene expression with the aim of identifying genes specifically associated with RCC. Using a modified DD-PCR approach, which was non-radioactive, quicker and simpler than the conventional method, 24 cDNA samples were clearly up- or down-regulated in RCC tissue from 4 patients. Fourteen of these showed high similarity to a number of known genes. Eight of these cDNA clones were chosen for further analysis. These were a regulator of G-protein signalling (RGS-5), Notch-3, Na,K-ATPase alpha subunit, HLA class II antigen, ETS-like protein, transforming growth factor beta-stimulated clone (TSC-22), bladder cancer-related protein (BC10) and adipophilin. Semi-quantitative RT-PCR using specific primers to each of these genes confirmed differential expression in 67% to 83% of a further 12 RCC and normal kidney paired samples from 7 of the 8 cDNA clones. Northern analysis further confirmed the up-regulation in expression of RGS-5 and Notch-3 in RCC. Further characterisation of these differentially expressed genes should lead to a better understanding of the changes that occur at the molecular level during RCC development and progression.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Rae FK,Stephenson SA,Nicol DL,Clements JAdoi
10.1002/1097-0215(20001201)88:5<726::aid-ijc7>3.0.keywords:
subject
Has Abstractpub_date
2000-12-01 00:00:00pages
726-32issue
5eissn
0020-7136issn
1097-0215pii
10.1002/1097-0215(20001201)88:5<726::AID-IJC7>3.0.journal_volume
88pub_type
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