Abstract:
:An important aspect of the cytochrome c electrochemistry is the possibility of coupling the 'heterogeneous reactions' with other redox enzymes. Cellobiose dehydrogenase, a 89170 Da glycoprotein that contains both FAD and a b-type haem as prosthetic groups, donates electrons to a number of acceptors, including cytochrome c. While haem b is surrounded mainly by acidic amino acids, cytochrome c displays positive charged lysine groups around the haem site. Thus a fast reaction between both proteins is explicable. In the presence of cellobiose, a catalytic current was observed, owing to the interaction of cellobiose dehydrogenase with electrostatically adsorbed cytochrome c. Adsorption of cytochrome c provides a technological model surface for vectorial electron transfer.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Fridman V,Wollenberger U,Bogdanovskaya V,Lisdat F,Ruzgas T,Lindgren A,Gorton L,Scheller FWdoi
10.1042/bst0280063keywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
63-70issue
2eissn
0300-5127issn
1470-8752journal_volume
28pub_type
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