Abstract:
:Genomic DNA is constantly under threat from intracellular and environmental factors that damage its chemical structure. Uncorrected DNA damage may impede cellular propagation or even result in cell death, making it critical to restore genomic integrity. Decades of research have revealed a wide range of mechanisms through which repair factors recognize damage and co-ordinate repair processes. In recent years, single-molecule live-cell imaging methods have further enriched our understanding of how repair factors operate in the crowded intracellular environment. The ability to follow individual biochemical events, as they occur in live cells, makes single-molecule techniques tremendously powerful to uncover the spatial organization and temporal regulation of repair factors during DNA-repair reactions. In this review, we will cover practical aspects of single-molecule live-cell imaging and highlight recent advances accomplished by the application of these experimental approaches to the study of DNA-repair processes in prokaryotes.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Ghodke H,Ho H,van Oijen AMdoi
10.1042/BST20170055subject
Has Abstractpub_date
2018-02-19 00:00:00pages
23-35issue
1eissn
0300-5127issn
1470-8752pii
BST20170055journal_volume
46pub_type
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journal_title:Biochemical Society transactions
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