Abstract：
:Gram negative bacterial infection is a leading cause of fatality and is attributed, at least in part, to the bacteria's capacity to persist in the host in spite of appropriate antibiotic therapy. It has been suggested that bacteria evade antibiotics by hiding within host cells. We sought to investigate this important aspect of infections in mast cells, which are inflammatory cells found in close proximity to the host-environment interface and which have recently been reported to play a crucial role in the early innate immune response to bacteria. We examined mast cell interactions with FimH-expressing E. coli, one of the major opportunistic pathogens of humans. We determined that in serum free conditions, these bacteria were able to trigger mast cell uptake without loss of bacterial viability. CD48, a mannose containing GPI (glycosylphosphatidylinositol)-linked molecule was found to be the receptor of FimH-expressing E. coli in mouse mast cells. We found that the internalization via CD48 was blocked by filipin, a cholesterol binding drug known to disrupt cholesterol/glycolipid-enriched microdomains and the bacteria-encasing vacuoles were rich in cholesterol inside cells. Interestingly, we found that mast cells subsequently expelled majority of the intracellular bacteria in 24 hours. This expulsion process was blocked by lovastatin/cyclodextrin treatment, which is known to inhibit cellular trafficking of cholesterol/glycolipid-enriched microdomains. Thus, the bacterial entry into and expulsion from mast cells were critically dependent on cholesterol/glycolipid-enriched microdomains, which represents a novel mode of tussle between the pathogen and the mast cell occurring in opsonin deficient sites in the body or even at other sites in naive or immunocompromised hosts which have low systemic levels of E. coli specific antibody.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Shin JS,Gao Z,Abraham SNdoi
10.1023/a:1020216323271keywords:
subject
Has Abstractpub_date
1999-10-01 00:00:00pages
421-32issue
5eissn
0144-8463issn
1573-4935journal_volume
19pub_type
杂志文章abstract：:Ovarian cancer is a highly lethal cancer in females. Therefore, it is necessary to explore effective biomarkers for the diagnosis and prognosis of the disease. Stratifin (SFN) is a cell cycle checkpoint protein that has been reported to be involved in oncogenesis. Our studies detected the expression of SFN in ovarian ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20190100
更新日期：2019-05-02 00:00:00
abstract：:The increase in % phosphorylase a associated with muscle contraction is reversed after a few minutes despite continued contractile activity. The present study was undertaken to determine whether adrenaline can activate phosphorylase after deactivation has occurred during prolonged stimulation of contraction. We found ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01115118
更新日期：1982-05-01 00:00:00
abstract：:Three genotypically suppressible alleles, a1X4, alcA125, and niaD500, are phenotypically suppressed by aminoglycoside antibiotics. Unsuppressible alleles at these loci are unaffected as are known missense mutations at the yA and gdhA loci. This is consistent with the premise that the suppressible mutations are nonsens...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01121566
更新日期：1983-01-01 00:00:00
abstract：:To clarify the mechanism of circGOLPH3 regulation on prostate cancer cells, we performed an overexpression and interference circGOLPH3 assay in prostate cancer cells PC-3 and then evaluated cellular viability, proliferation, cell cycle, and apoptosis of prostate cancer cells by MTT, CCK8, Edu stain, TUNEL stain, and f...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20200936
更新日期：2020-12-23 00:00:00
abstract：:Native bovine parathyroid hormone (bPTH) was found to be readily cleaved with human leukocyte elastase to yield the fragments bPTH(1-41) and bPTH(42-84). These were then isolated by reverse-phase HPLC and characterised by gas-phase sequencing and amino acid analysis. The biological activities of these fragments were a...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01122726
更新日期：1987-01-01 00:00:00
abstract：:Bladder cancer is a common malignant tumour worldwide. Epithelial-mesenchymal transition (EMT)-related biomarkers can be used for early diagnosis and prognosis of cancer patients. To explore, accurate prediction models are essential to the diagnosis and treatment for bladder cancer. In the present study, an EMT-relate...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20203944
更新日期：2021-01-29 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01120989
更新日期：1983-08-01 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01116509
更新日期：1987-06-01 00:00:00
abstract：:Identified and cloned in 1996 for the first time, G protein-coupled oestrogen receptor (ER) 30 (GPR30/GPER) has been a hot spot in the field of sex hormone research till now. In the present study, we examined the effects of low-dose oestradiol (E2) combined with G15, a specific antagonist of GPR30 on ovariectomy (OVX)...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20150146
更新日期：2015-07-03 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01117344
更新日期：1988-12-01 00:00:00
abstract：:Circulating tumor cells can provide important diagnostic and prognostic information of the patients with non-small cell lung cancer (NSCLC). Aldehyde dehydrogenase 1 (ALDH1), a cancer stem cell marker, has been used in various tumors, including NSCLC. In the present study, we isolated the circulating ALDH1+ tumor cell...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20180914
更新日期：2018-10-31 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20181658
更新日期：2019-03-22 00:00:00
abstract：OBJECTIVES:To investigate the association between dectin-1 gene single nucleotide polymorphisms (SNPs) and susceptibility to fungal infection (FI). METHODS:Databases were searched electronically and manually to identify case-control studies concerning dectin-1 SNPs and FI, which were published up to 12 November 2018. ...
journal_title：Bioscience reports
pub_type： 杂志文章,meta分析
doi：10.1042/BSR20191519
更新日期：2019-11-29 00:00:00
abstract：:Homologues of the putative dehydrogenase YjhC are found in operons involved in the metabolism of N-acetylneuraminate (Neu5Ac) or related compounds. We observed that purified recombinant YjhC forms Neu5Ac from two dehydrated forms of this compound, 2,7-anhydro-N-acetylneuraminate (2,7-AN) and 2-deoxy-2,3-didehydro-N-ac...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20200927
更新日期：2020-06-26 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01182482
更新日期：1991-06-01 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章,评审
doi：10.1023/a:1016061006256
更新日期：2002-02-01 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20160226
更新日期：2016-11-03 00:00:00
abstract：:Polymorphisms of methylenetetrahydrofolate reductase (MTHFR) in hormone metabolism pathways might cause metabolic disturbances and contribute to the development of polycystic ovary syndrome (PCOS) and ovarian cancer, but the published studies were inconsistent. The aim of the present study was to evaluate the MTHFR C6...
journal_title：Bioscience reports
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doi：10.1042/BSR20200995
更新日期：2020-07-31 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1023/a:1014768909442
更新日期：2001-10-01 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20190504
更新日期：2019-06-18 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章,评审
doi：10.1023/a:1020182404940
更新日期：2002-04-01 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20181320
更新日期：2018-09-07 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01114948
更新日期：1986-06-01 00:00:00
abstract：BACKGROUND:Glioma is the most common malignant tumor in the human central nervous system. Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) promotes oncogenesis in various tumors. In the present study, we aimed to examine the role of NEAT1 in altering the properties of gliomas. METHODS:Quantitative ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20200767
更新日期：2021-01-04 00:00:00
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journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1042/BSR20201482
更新日期：2020-12-01 00:00:00
abstract：:Superoxide dismutase (SOD), a metal containing enzyme is present in parasite Leishmania donovani as well as in host macrophages both resident and activated in a detectable amount, although the level is much higher in the latter case. It is observed that at any particular protein concentration, the SOD activity is high...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01116457
更新日期：1988-04-01 00:00:00
abstract：:The influence of lindane upon the dynamic properties of plasma membranes from rat ventral prostate has been investigated using a fluorescence polarization technique. Preincubation with lindane decreased the fluorescence polarization in a dose dependent manner. This effect, which is associated with an increased membran...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01119197
更新日期：1991-04-01 00:00:00
abstract：:The family of 5-HT4 receptors comprises 16 putative splice variants. We have previously shown that there are differences in signal transduction of the h5-HT(4a) and h5-HT(4b) receptors. In the present study, the internalization of these two splice variants following receptor stimulation was investigated with confocal ...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/s10540-005-2582-5
更新日期：2004-06-01 00:00:00
abstract：:125I-insulin was shown to be internalized in vivo to a discrete population of low-density membranes (ligandosomes), distinct from the +Golgi, endoplasmic reticulum, plasma membrane, and lysosomes. However, analytical subcellular fractionation shows that glutathione-insulin transhydrogenase is localized to the endoplas...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01122896
更新日期：1983-04-01 00:00:00
abstract：:Cathepsin D occurs in two forms, a single polypeptide chain (Mr 44 000) and a non-covalent complex of two peptides of Mr 14 000 and 30 000 that is derived by proteolytic processing of the 44 000 polypeptide. The two forms from bovine spleen are closely similar in secondary structure content, in aromatic amino acid env...
journal_title：Bioscience reports
pub_type： 杂志文章
doi：10.1007/BF01119908
更新日期：1985-10-01 00:00:00
