Abstract：
:Measurement of lymphocyte proliferation to detect hypersensitivity to beryllium (Be-LPT) in vitro is done presently using a method based on tritiated thymidine incorporation. Although this method is sensitive it gives no information on cell viability or responding lymphocyte subsets. We have developed reliable and simple flow cytometric assays for lymphocyte proliferation testing (Immuno-Be-LPT) by combining immunophenotyping with bromodeoxyuridine (BrdU) incorporation or DNA content using propidium iodide (PI) or 4'6'-diimidazolin-2-phenylindole (DAPI). Evaluation of beryllium-induced lymphocyte proliferation in blood cells from seven patients with chronic beryllium disease (CBD) and 120 beryllium workers by both the Bc-LPT and the Immuno-Be-LPT showed agreement between the tests. The Immuno-Bc-LPT provided additional information about the specific type of lymphocytes responding. CD4+ lymphocytes proliferated in response to beryllium in blood samples from all seven CBD individuals and CD8+ lymphocytes proliferated in six of the seven. Four beryllium workers without CBD had positive responses to beryllium primarily in the CD8+ cells. The use of the individual's own plasma supported a greater beryllium or tetanus-induced proliferation of CD4+ lymphocytes when compared to commercial human serum. The response of CD4+ lymphocytes measured in the Immuno-Be-LPT may provide a new marker for the diagnosis of CBD.
journal_name
Toxicologyjournal_title
Toxicologyauthors
Farris GM,Newman LS,Frome EL,Shou Y,Barker E,Habbersett RC,Maier L,Smith HN,Marrone BLdoi
10.1016/s0300-483x(99)00167-5keywords:
subject
Has Abstractpub_date
2000-02-21 00:00:00pages
125-40issue
2eissn
0300-483Xissn
1879-3185pii
S0300-483X(99)00167-5journal_volume
143pub_type
杂志文章相关文献
TOXICOLOGY文献大全abstract：:The subacute toxicity of sodium saccharin and 2 hydrolytic derivatives, o-sulfamoylbenzoic acid (Compound I) and ammonium o-carboxybenzene sulfonate (Compound II) was evaluated by feeding each of the compounds alone at a dietary level of 20 000 ppm to both beagle dogs and albino rats. Additionally, groups of dogs and ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(76)90015-9
更新日期：1976-08-01 00:00:00
abstract：:The usefulness of measuring serum bile acid concentrations by RIA in a number of acute experimental liver injuries of rats was assessed by comparing the concentrations with the results of some of the routinely employed methods of examining hepatotoxic changes. Centrilobular liver cell injury produced by CCl4 revealed ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(80)90005-0
更新日期：1980-01-01 00:00:00
abstract：:Although manufactured magnetic nanoparticles (NPs) are currently used in many fields, NPs have potential toxicity on cardiovascular system especially atherosclerosis. In our previous study, we prepared novel Fe3O4 nanoparticles surface-coated with aminoguanidine (Fe3O4-AG NPs) which could remove acid dyes from aqueous...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2019.03.002
更新日期：2019-05-01 00:00:00
abstract：:Mitochondria are cellular powerhouses as well as metabolic and signaling hubs regulating diverse cellular functions, from basic physiology to phenotypic fate determination. It is widely accepted that reactive oxygen species (ROS) generated in mitochondria participate in the regulation of cellular signaling, and that s...
journal_title：Toxicology
pub_type： 杂志文章,评审
doi：10.1016/j.tox.2017.07.013
更新日期：2017-11-01 00:00:00
abstract：:Tetrahydrocannabinol (THC), a psychoactive component of marijuana, is known to exert cytotoxicity in immune cells. In the present study, we examined the cytotoxicity of Δ⁸-THC in mouse macrophage J774-1 cells and a possible involvement of cannabinoid receptors and stress-responsive mitogen-activated protein kinases (M...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2013.10.007
更新日期：2013-12-15 00:00:00
abstract：:The Internet = s global reach offers new powerful tools to professionals in Occupational and Environmental Health (OEH). The World Wide Web includes extensive free and commercially available reference materials on toxicology, regulatory issues, environmental epidemiology and prevention programs. Much of this especiall...
journal_title：Toxicology
pub_type： 杂志文章,评审
doi：10.1016/s0300-483x(02)00028-8
更新日期：2002-04-25 00:00:00
abstract：:Chemical carcinogens are generally classified as genotoxic or non-genotoxic. However, weak genotoxicity at high concentrations is sometimes observed and interpretation is often problematic. In addition, certain rodent carcinogens exert their effects at doses associated with cytotoxicity and compensatory hyperplasia ma...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(03)00058-1
更新日期：2003-05-03 00:00:00
abstract：:In mice, styrene is hepatotoxic, pneumotoxic, and causes lung tumors. One explanation for the mechanism of toxicity is oxidative stress/damage. Previous studies have shown decreased glutathione levels, linked to increased apoptosis, in lung homogenates and isolated Clara cells 3 h following styrene or styrene oxide (S...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2009.08.001
更新日期：2009-10-29 00:00:00
abstract：OBJECTIVE:We investigated the potential dose related apoptotic effect of subchronic administration of the alkylating chemotherapeutic agent ifosfamide in different sites of the small and large rabbit intestine. MATERIALS AND METHODS:Twenty New Zealand White rabbits received intravenously every week, for 10 weeks, 0 (g...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2004.03.020
更新日期：2004-08-05 00:00:00
abstract：:Cadmium is a heavy metal, which is widely used in industry, affecting human health through occupational and environmental exposure. In mammals, it exerts multiple toxic effects and has been classified as a human carcinogen by the International Agency for Research on Cancer. Cadmium affects cell proliferation, differen...
journal_title：Toxicology
pub_type： 杂志文章,评审
doi：10.1016/s0300-483x(03)00305-6
更新日期：2003-11-05 00:00:00
abstract：:Between the DNA sequences of two randomly-selected human genomes, which consist of over 3 billion base pairs and twenty five thousand genes, there exists only 0.1% variation and 99.9% sequence identity. During the last couple of decades, extensive genome-wide studies have investigated the association between single-nu...
journal_title：Toxicology
pub_type： 杂志文章,评审
doi：10.1016/j.tox.2017.03.020
更新日期：2017-05-15 00:00:00
abstract：:The antidotal property of oximes is attributed to their ability to reactivate acetylcholinesterase (AChE) inhibited by organophosphorus compounds (OP) such as pesticides and nerve warfare agents. Understanding their interactions within the active site of phosphylated AChE is of great significance for the search for mo...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2018.05.008
更新日期：2018-08-01 00:00:00
abstract：:Our previous study revealed that exposure to 0.4 ppm ozone (O(3)) enhanced nasal allergy-like reactions in guinea pigs. In the present study, we investigated the concentration-dependency of the effects of exposure to O(3) on the aggravation of nasal allergy-like reactions induced by repeated nasal administration of an...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2004.01.008
更新日期：2004-06-01 00:00:00
abstract：:The in vivo effects of sodium cyanide and its antidotes, sodium nitrite, sodium thiosulfate and 4-dimethylaminophenol (DMAP), as well as the alpha-adrenergic blocking agent phentolamine, on rat brain cytochrome oxidase were studied. The course of inhibition was time-dependent and a peak of 40% was attained between 15 ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(92)90018-a
更新日期：1992-11-22 00:00:00
abstract：:The histogenesis of coumarin-induced cholangiofibrosis in the rat has been determined. Proliferation of ductal structures was preceded by extensive damage to hepatocytes in the centrilobular region. Focal proliferation of ducts and fibrous tissue was present at 3 months and typical areas of cholangiofibrosis at 6 mont...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(89)90187-x
更新日期：1989-04-01 00:00:00
abstract：:Osteotoxic compounds administered during pregnancy can initiate skeletal congenital anomalies in the embryo. In vitro, developmental osteotoxicity of a compound can be predicted with the embryonic stem cell test (EST), the only in vitro embryotoxicity model identified to date that entirely abrogates the use of animals...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2010.08.016
更新日期：2010-11-09 00:00:00
abstract：:Rats were exposed to isopropanol by inhalation of 200,2000 and 8000 ppm for 2 weeks with a daily exposure of 6 h. A pilot group exposed to 8000 ppm was given a recovery period of 4 weeks. Kidney and liver microsomal metabolism of n-hexane was investigated in vitro concomitant with activities of cytochrome P-450 and GS...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(85)90078-2
更新日期：1985-01-01 00:00:00
abstract：:Novel substituted phenoxyalkyl pyridinium oximes, previously shown to reactivate brain cholinesterase in rats treated with high sublethal dosages of surrogates of sarin and VX, were tested for their ability to prevent mortality from lethal doses of these two surrogates. Rats were treated subcutaneously with 0.6mg/kg n...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2015.12.001
更新日期：2016-01-02 00:00:00
abstract：:Indoor suspended particulate matter (SPM) consists of many different types of particles, the vast majority of which are less than 2.5 microm in diameter. An important question is how these particles, being inhalable, contribute to asthma and respiratory symptoms. One possibility is that these particles have an adjuvan...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(98)00079-1
更新日期：1998-08-21 00:00:00
abstract：:Nine structurally different polycyclic aromatic hydrocarbons (PAHs) were tested for their ability to either agonize or antagonize the human androgen receptor (hAR) in a sensitive reporter gene assay based on CHO cells transiently cotransfected with a hAR vector and an MMTV-LUC vector. Benz[a]anthracene (B[a]A), benzo[...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(00)00143-8
更新日期：2000-04-14 00:00:00
abstract：:The mechanism of delta-aminolevulinate dehydratase (delta-ALA-D) inhibition by phenyl selenoacetylene in vitro was investigated in this study. Phenyl selenoacetylene (40-400 microM) inhibition of delta-aminolevulinate dehydratase from rat liver (low speed supernatant fraction, S1 fraction) was partially prevented by i...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2004.08.001
更新日期：2005-01-31 00:00:00
abstract：:The term "health/functional food" (HFF) refers to food supplements containing nutrients or other substances (in a concentrated form) that have a nutritional or physiological effect whose purpose is to supplement the normal diet. The Korean Health/Functional Food Act that came into effect in 2004 requires these product...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2006.01.016
更新日期：2006-04-03 00:00:00
abstract：:During the past decade, several assessments have estimated the carcinogenic risk of inhaled 1,3-butadiene (BD) to the general population as well as for occupational exposures. Although most of the risk assessments have been based on inhalation bioassays in mice, some of the assessments have included the 2-year inhalat...
journal_title：Toxicology
pub_type： 杂志文章,评审
doi：10.1016/0300-483x(96)03447-6
更新日期：1996-10-28 00:00:00
abstract：:In the present study, we investigated the inducibility of the drug conjugate transporter genes MRP1 and MRP2 by redox-active compounds such as tertiary butylated hydroquinone (tBHQ) and quercetin and by chemicals known to activate the pregnane X receptor (PXR) such as rifampicin and clotrimazol and by the metalloid co...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(01)00570-4
更新日期：2002-02-28 00:00:00
abstract：:The effect of dose and duration of treatment with chromium on its distribution in certain vital organs and the blood of rats has been investigated. The accumulation of metal was highest in liver and kidneys, followed by testes, brain and blood and was rather more dependent on the dose than on the duration of exposure....
journal_title：Toxicology
pub_type： 杂志文章
doi：
更新日期：1979-05-01 00:00:00
abstract：:Methylcyclopentadienyl manganese tricarbonyl (MMT) is an effective inhibitor of mitochondrial respiration associated with NAD+-linked substrates. At concentrations which inhibit glutamate-malate oxidation (over 80% inhibition) succinate and tetramethylphenylenediamine (TMPD)-ascorbate are inhibited less than 25%. MMT ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(77)90043-9
更新日期：1977-02-01 00:00:00
abstract：:The toxic oil syndrome (TOS), a multisystemic disease, that occurred in Spain in 1981, was caused by the ingestion of rapeseed oil denatured with 2% aniline. Due to the clinical course of the disease, immunopathological mechanisms have been suspected but a direct connection was never demonstrated. To analyse this poss...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(94)90085-x
更新日期：1994-11-11 00:00:00
abstract：:The presence of inclusion bodies in renal proximal tubules was studied in rats exposed to lead and/or disulfiram (tetraethylthiuram disulfide). Pregnant rats were treated with only lead acetate (0.25% Pb in the drinking water), only disulfiram (0.1 mmol/kg p.o. twice a week) or with both lead acetate and disulfiram fr...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(87)90046-1
更新日期：1987-04-01 00:00:00
abstract：:Recent studies have demonstrated that mammalian sperm are capable of generating reactive oxygen species (ROS) and that this activity is significantly accelerated in subfertile subjects. The observed decrease in penetration of zona-intact oocyte might be explained by chemical-induced ROS-related early onset of capacita...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(99)00107-9
更新日期：1999-11-29 00:00:00
abstract：:A representative reactive oxygen species (ROS), hydroxyl radical (*OH), is a highly reactive species and induces DNA backbone breakage. *OH also oxidizes every DNA base. The interaction of *OH with guanine leads to the generation of not only piperidine-resistant 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) but also v...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2006.01.002
更新日期：2006-04-17 00:00:00
