Abstract:
:Poly(D,L-lactic-co-glycolic) acid (PLGA) microspheres containing plasmid DNA encoding the firefly luciferase gene were prepared using the water-in-oil-in-water (w/o/w) double emulsion and solvent evaporation method. In this study, we investigated the effects of three process parameters on DNA microencapsulation: (1) emulsification method used to generate the primary emulsion, (2) water/oil ratio during formation of the first emulsion, and (3) surfactant concentration used in the preparation of the second emulsion. The resulting formulations were also analyzed for microsphere size, encapsulation efficiency, and kinetics of DNA release. We found that although each process alteration resulted in encapsulation of biologically active, structurally intact DNA, the surfactant and water/oil ratio significantly affected the size, release kinetics and encapsulation efficiency of plasmid DNA.
journal_name
J Drug Targetjournal_title
Journal of drug targetingauthors
Hsu YY,Hao T,Hedley MLdoi
10.3109/10611869909085514keywords:
subject
Has Abstractpub_date
1999-12-01 00:00:00pages
313-23issue
4eissn
1061-186Xissn
1029-2330journal_volume
7pub_type
杂志文章abstract::The myriad of complications associated with diabetes is closely linked with the generation of reactive species or free radicals leading to oxidative and nitrosative stress. Increased oxidative stress is an important cause and result of diabetes and it is thought to underlie the cellular changes that lead to diabetic c...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.1080/1061186X.2017.1419478
更新日期:2018-11-01 00:00:00
abstract::beta-Glucuronidase (GUSB) is a lysosomal enzyme that could be developed as a brain therapy for Type VII Mucopolysaccharidosis. However, GUSB does not cross the blood-brain barrier (BBB). To enable BBB transport of the enzyme, human GUSB was re-engineered as a fusion protein with the chimeric monoclonal antibody (MAb) ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860903353362
更新日期:2010-04-01 00:00:00
abstract:BACKGROUND:Synthesized dl-Nordihydroguaiaretic acid (dl-NGDA or "Nordy") can inhibit the growth of malignant human tumors, especially the tumor angiogenesis. However, its liposoluble nature limits its in vivo efficacy in the hydrosoluble circulation of human. PURPOSE:We tried to use the ultrasonic microbubble as the c...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2016.1144058
更新日期:2016-09-01 00:00:00
abstract::Liposomes have been prepared loaded with DNA (plasmid encoding for the green fluorescent protein, GFP) and additionally modified with TATp and PEG, with PEG being attached to the liposome surface via both pH-sensitive hydrazone and non-pH-sensitive bonds. The pGFP-loaded liposomal preparations have been administered i...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860701498203
更新日期:2007-08-01 00:00:00
abstract::Drug efflux by the membrane transporter P-glycoprotein (P-gp) plays a key role in multidrug resistance (MDR). In order to bypass P-gp, thus overcoming MDR, a hybrid peptide comprising a cell penetrating peptide (Tat) and a drug binding motif (DBM) has been developed to noncovalently bind and deliver doxorubicin (Dox) ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860903548347
更新日期:2010-07-01 00:00:00
abstract::In vitro transfection efficiency of a plasmid DNA for rat gastric mucosal (RGM)-1 cells was enhanced by ultrasound (US) irradiation. Ethylenediamine was introduced to the carboxyl groups of gelatin to prepare a cationized gelatin as the vector of plasmid DNA encoding luciferase. An electrophoresis experiment revealed ...
journal_title:Journal of drug targeting
pub_type: 杂志文章,收录出版
doi:10.1080/10611860290022624
更新日期:2002-05-01 00:00:00
abstract::The transport of 125I-radiolabelled latex nanoparticles across the nasal mucosa of rats was studied using a range of particle sizes and surface coatings. Translocation of the particles into the blood stream was examined by means of monitoring the radiolabel associated with the particles. Particles were detected in the...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860108997935
更新日期:2001-01-01 00:00:00
abstract::As a percutaneous sustained-release preparation, insulin micropiles (MPs) were prepared with biodegradable polymers poly(lactic acid) (PLA), poly(ϵ-caprolactone) (PCL) and poly(lactic-co-glycolic acid) (PLGA) as the base. The obtained PLA, PCL, and PLGA MPs of which the insulin:polymer ratio was 1:2 were administered ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2010.492521
更新日期:2011-04-01 00:00:00
abstract::In order to confirm the efficacy of ciprofloxacin (CPFX) incorporated into liposomes (CPFX-liposomes) for treatment of respiratory intracellular parasite infections, the influence of particle size on drug delivery to rat alveolar macrophages (AMs) following pulmonary administration of CPFX-liposomes was investigated. ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860600834375
更新日期:2006-09-01 00:00:00
abstract::Necrosis avid agents (NAAs) can be used for diagnose of necrosis-related diseases, evaluation of therapeutic responses and targeted therapeutics of tumor. In order to probe into the effects of molecular skeleton structure on necrosis targeting and clearance properties of radioiodinated dianthrones, four dianthrone com...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2015.1113541
更新日期:2016-01-01 00:00:00
abstract::Diabetes is a condition that is not completely treatable but life of a diabetic patient can be smoothed by preventing or delaying the associate conditions like diabetic retinopathy, nephropathy, impaired wound healing process, etc. Apart from conventional methods to regulate diabetic condition, new techniques using si...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.1080/1061186X.2018.1476518
更新日期:2019-02-01 00:00:00
abstract::The present investigation was aimed at targeting indinavir, a protease inhibitor to cells of mononuclear phagocyte system (MPS) via mannosylated liposomes. β-d-1-thiomannopyranoside residues were covalently coupled with dimyristoyl phosphatidylethanolamine (DMPE) to generate mannosylated-DMPE (Man-DMPE) conjugate whic...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2010.499460
更新日期:2011-05-01 00:00:00
abstract::Pathophysiological molecules in the extracellular environment offer excellent targets that can be exploited for designing drug targeting systems. Matrix metalloproteases (MMPs) are a family of extracellular proteolytic enzymes that are characterized by their overexpression or overactivity in several pathologies. Over ...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.1080/10611860600968967
更新日期:2007-01-01 00:00:00
abstract::The human papillomavirus type-16 (HPV-16) E7 protein is considered a major viral oncoprotein involved in cervical cancer (CxCa) and a potential candidate for the development of a vaccine against this neoplasia. Here, two lactic acid bacteria (the model one Lactococcus lactis and a probiotic one Lactobacillus plantarum...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860400024219
更新日期:2005-02-01 00:00:00
abstract::We have previously reported that the transfection efficiency of laminin-derived AG73-peptide labeled polyethyleneglycol-modified liposomes (AG73-PEG liposomes) was enhanced by echo-contrast gas entrapping PEG liposomes (Bubble liposomes, BLs) and ultrasound (US) exposure by improving endosomal escape. However, it has ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2012.660162
更新日期:2012-05-01 00:00:00
abstract:OBJECTIVE:The objective of this work was to examine the application of catechins-containing phospholipids-based ultrasonic microbubbles (PUM) in ultrasound-induced delivery. METHODS:Catechins-containing PUM were prepared by dissolving lyophilized PUM powder in catechins solution. Morphologic characteristics of catechi...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860802475696
更新日期:2008-12-01 00:00:00
abstract::DNA nanoparticles (DNA NPs), which self-assemble from DNA plasmids and poly-L-lysine (pLL)-polyethylene glycol (PEG) block copolymers, transfect several cell types in vitro and in vivo with minimal toxicity and immune response. To further enhance the gene transfer efficiency of DNA NP and control its tropism, we estab...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2012.712128
更新日期:2012-09-01 00:00:00
abstract::Notch pathway was found to be activated in most glioblastomas (GBMs), underlining the importance of Notch in formation and recurrence of GBM. In this study, a Notch inhibitory peptide, dominant negative MAML (dnMAML), was conjugated to elastin-like polypeptide (ELP) for tumor targeted delivery. ELP is a thermally resp...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186X.2017.1289537
更新日期:2017-07-01 00:00:00
abstract::The human body has adapted to defend against the aggressive biological or chemical agents. As a result, the defence mechanisms of the human body became barriers for the drug delivery. Theoretically, any problem that prevents a drug from reaching its site of action is considered to be a barrier to drug delivery. The ai...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860903398099
更新日期:2010-05-01 00:00:00
abstract::Gelatin microspheres (ms) and gelatin/BSA (bovine serum albumin) or gelatin/alginate ms were prepared by encapsulating fluorescein isothiocyanate (FITC) labeled dextran or interferon alpha (IFN-alpha). Ms were obtained by an emulsion-solvent-extraction method. Gelatin and gelatin/BSA ms were obtained by treating water...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869908996851
更新日期:1999-01-01 00:00:00
abstract::Radiolabeled cytochrome c (Cyt c), either as a free protein or as cell penetrating peptide (CPP)-conjugates, was tested for cellular uptake and nuclear transport in Human embryonic kidney 293 (HEK293) cells and HeLa cells. Conjugation of Cyt c with either the amphipathic peptide model amphipathic peptide (MAP) or the ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2011.649481
更新日期:2012-05-01 00:00:00
abstract::A nuclear localization signal (NLS) is a short amino acid sequence derived from eukaryotic nuclear proteins and viral proteins. Many NLS peptides can efficiently mediate the intranucleus transport of cargo molecules, so they have been widely used for non-viral gene transfer and shown potential ability to improve nucle...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.1080/1061186X.2016.1184273
更新日期:2016-12-01 00:00:00
abstract::Cancer is one of the most common diseases all over the world; many people suffer from diverse types of cancer. However, currently there is no exact cure or therapy developed for cancer. On the other hand, nanoparticles are defined as microscopic particles that have dimensions less than 100 nm and they are known for th...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.1080/1061186X.2018.1527338
更新日期:2019-08-01 00:00:00
abstract::A higher encapsulation rate was obtained using the dehydration-rehydration method compared with the reverse-phase evaporation technique in negative multilamellar vesicles with amikacin (AMK) (45% versus 15%; P < 0.05) and teicoplanin (TCP) (34% versus 25%; P < 0.05). The addition of 250 mM sucrose to AMK- or TCP-conta...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869308996076
更新日期:1993-01-01 00:00:00
abstract::The purpose of the study was to develop the liposomal formulations of TFu for oral and intravenous (i.v.) administration, clarify the biodistribution characteristics and in vivo pharmacokinetic behaviors of TFu-loaded liposomes. Four TFu-loaded liposomes of different sizes were prepared and characterized. The pharmaco...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860801927598
更新日期:2008-06-01 00:00:00
abstract::Based on the organotropic characteristics of bile acids towards the liver and the intestine, two novel compounds of the Bamet family, containing at least one bile acid moiety bound to platinum(II), have been synthesized and their cytostatic effect compared to their ability to become accumulated in tumor cells of hepat...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186021000001841
更新日期:2002-08-01 00:00:00
abstract::Cytokine inhibiting drugs are much more effective when delivered intracellularly to phagocytic cells in the microencapsulated form. Dexamethasone is a powerful inhibitor of TNF-α cytokine through inhibition of NF-κB which is a gene regulator of multiple pro-inflammatory cytokines. We have determined the effect of micr...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2011.561856
更新日期:2011-11-01 00:00:00
abstract:OBJECTIVE:Histone deacetylases (HDACs) have been revealed to be involved in cerebrovascular diseases, while the role of HDAC9 in intracranial aneurysm (IA) remains seldom studied. We aim to explore the role of the HDAC9/microRNA-92a (miR-92a)/Bcl-2-like protein 11 (BCL2L11) axis in IA progression. METHODS:Expression o...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186X.2021.1878365
更新日期:2021-01-22 00:00:00
abstract:BACKGROUND:High expression of glucagon-like peptide-1 receptor (GLP-1R) in insulinoma supplies a potential drug target for tumor imaging. Exendin-4 can specifically bind to GLP-1R as an agonist and its analogs are extensively used in receptor imaging studies. PURPOSE:A new GLP-1R imaging agent, [(18)F]AlF-NOTA-MAL-Cys...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2015.1020808
更新日期:2015-01-01 00:00:00
abstract::This study was aimed to generate a new agent, norcantharidin (NCTD) encapsulated liposomes modified with a novel murine anti-human CD19 monoclonal antibody 2E8 (2E8–NCTD–liposomes), to specifically target the B-lineage leukemia stem cells (B-LSCs) and their progeny in vitro. Our results have shown that the positive pe...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611861003649720
更新日期:2010-11-01 00:00:00