Abstract:
:Liposomes have been prepared loaded with DNA (plasmid encoding for the green fluorescent protein, GFP) and additionally modified with TATp and PEG, with PEG being attached to the liposome surface via both pH-sensitive hydrazone and non-pH-sensitive bonds. The pGFP-loaded liposomal preparations have been administered intratumorarly in tumor-bearing mice and the efficacy of tumor cell transfection was followed after 72 h. The administration of pGFP-TATp-liposomes with non-pH-sensitive PEG coating has resulted in only minimal transfection of tumor cells because of steric hindrances for the liposome-to-cell interaction created by the PEG coat, which shielded the surface-attached TATp. At the same time, the administration of pGFP-TATp-liposomes with the low pH-detachable PEG resulted in at least three times more efficient transfection since the removal of PEG under the action of the decreased intratumoral pH leads to the exposure of the liposome-attached TATp residues, enhanced penetration of the liposomes inside tumor cells and more effective intracellular delivery of the pGFP. This result can be considered as an important step in the development of tumor-specific stimuli-sensitive drug and gene delivery systems.
journal_name
J Drug Targetjournal_title
Journal of drug targetingauthors
Kale AA,Torchilin VPdoi
10.1080/10611860701498203subject
Has Abstractpub_date
2007-08-01 00:00:00pages
538-45issue
7-8eissn
1061-186Xissn
1029-2330pii
780334291journal_volume
15pub_type
杂志文章abstract::The objective of this study was to investigate the factors for enhancing the susceptibility of cancer cells to chemotherapeutic drug by ultrasound microbubbles. Ultrasound (US) combined with phospholipid-based microbubbles (MB) was used to enhance the susceptibility of colon cancer cell line SWD-620 to anticancer drug...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860903434043
更新日期:2010-07-01 00:00:00
abstract::Gene transfer offers a potential way to correct local and systemic protein deficiency disorders by using genes as drugs, so called gene therapeutics. Salivary glands present an interesting target site for gene therapeutic applications. Herein, we review proofs of concept achieved for salivary glands with in vivo anima...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.3109/10611860108998782
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Synthesized dl-Nordihydroguaiaretic acid (dl-NGDA or "Nordy") can inhibit the growth of malignant human tumors, especially the tumor angiogenesis. However, its liposoluble nature limits its in vivo efficacy in the hydrosoluble circulation of human. PURPOSE:We tried to use the ultrasonic microbubble as the c...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2016.1144058
更新日期:2016-09-01 00:00:00
abstract::The purpose of the study was to develop the liposomal formulations of TFu for oral and intravenous (i.v.) administration, clarify the biodistribution characteristics and in vivo pharmacokinetic behaviors of TFu-loaded liposomes. Four TFu-loaded liposomes of different sizes were prepared and characterized. The pharmaco...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860801927598
更新日期:2008-06-01 00:00:00
abstract::Most therapeutic agents are delivered orally. Consequently, the major classes of therapeutically useful chemicals are partially lipophilic, small molecular weight compounds. They have reasonable permeability coefficient values across cell membranes, including those of intestinal epithelia and vascular endothelia. In c...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.1080/10611860410001693715
更新日期:2004-02-01 00:00:00
abstract::The objective of this study is to develop and evaluate gastroretentive particulate delivery systems using Riboflavin-5'-monophosphate sodium salt dihydrate (RF5'PNa) as model drug. Poly(acrylic acid)-cysteine and chitosan-4-thiobuthylamidine were evaluated and compared as anionic and cationic polymers for gastroretent...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860903483370
更新日期:2010-06-01 00:00:00
abstract::Polynucleotides are anionic macromolecules which are expected to transfer into the targeted cells through specific uptake mechanisms. So, we developed polynucleotides coating complexes of plasmid DNA (pDNA) and polyethylenimine (PEI) for a secure and efficient gene delivery system and evaluated their usefulness. Polya...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2014.950665
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:The objective of this work was to examine the application of catechins-containing phospholipids-based ultrasonic microbubbles (PUM) in ultrasound-induced delivery. METHODS:Catechins-containing PUM were prepared by dissolving lyophilized PUM powder in catechins solution. Morphologic characteristics of catechi...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860802475696
更新日期:2008-12-01 00:00:00
abstract::Liposomal encapsulation of doxorubicin (DXR) improves tumor accumulation and reduces adverse effects. One possible strategy for further optimization of this delivery technology would be to design the liposome carrier to release its content within the tumor tissue in response to specific stimuli such as ultrasound (US)...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2010.551401
更新日期:2011-09-01 00:00:00
abstract::Cytokine inhibiting drugs are much more effective when delivered intracellularly to phagocytic cells in the microencapsulated form. Dexamethasone is a powerful inhibitor of TNF-α cytokine through inhibition of NF-κB which is a gene regulator of multiple pro-inflammatory cytokines. We have determined the effect of micr...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2011.561856
更新日期:2011-11-01 00:00:00
abstract::Finding adequate carriers for proteins/peptides and anticancer drugs delivery has become an urgent need, owing to the growing number of therapeutic macromolecules and the increasing amount of cancer incidence. Polysaccharide-based nanogels have attracted interest as carriers for proteins/peptides and anticancer drugs ...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.1080/1061186X.2017.1326123
更新日期:2017-09-01 00:00:00
abstract::Necrosis avid agents (NAAs) can be used for diagnose of necrosis-related diseases, evaluation of therapeutic responses and targeted therapeutics of tumor. In order to probe into the effects of molecular skeleton structure on necrosis targeting and clearance properties of radioiodinated dianthrones, four dianthrone com...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2015.1113541
更新日期:2016-01-01 00:00:00
abstract::Germ-free (GF) animals lack a colonic microflora like that seen in conventional (CV) animals. Bacterial presence plays a role in the development of glycoproteins in the gastrointestinal (GI) tract; the absence of a microbiota has been seen to suppress the production of certain glycoproteins and glycolipids. Binding pa...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860108997920
更新日期:2001-04-01 00:00:00
abstract::Glioma is one of the most commonly occurring malignant brain tumours which need proper treatment strategy. The current therapies for treating glioma like surgical resection, radiotherapy, and chemotherapy have failed in achieving satisfactory results and this forms a rationale for the development of novel drug deliver...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.3109/1061186X.2014.916712
更新日期:2014-07-01 00:00:00
abstract:BACKGROUND:High expression of glucagon-like peptide-1 receptor (GLP-1R) in insulinoma supplies a potential drug target for tumor imaging. Exendin-4 can specifically bind to GLP-1R as an agonist and its analogs are extensively used in receptor imaging studies. PURPOSE:A new GLP-1R imaging agent, [(18)F]AlF-NOTA-MAL-Cys...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2015.1020808
更新日期:2015-01-01 00:00:00
abstract::Nanostructured lipid carrier (NLC) is second generation smarter drug carrier system having solid matrix at room temperature. This carrier system is made up of physiological, biodegradable and biocompatible lipid materials and surfactants and is accepted by regulatory authorities for application in different drug deliv...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.3109/1061186X.2012.716845
更新日期:2012-12-01 00:00:00
abstract::HEK cells were transfected with the GFP gene using various vectors: naked DNA, lipofectamine, and both PLGA and P(FASA) plasmid-loaded nanospheres. All methods were assessed alone and with the use of chloroquine, a lysosomal enzyme inhibitor. Transfection efficiencies were determined and compared at various times post...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186021000038373
更新日期:2002-09-01 00:00:00
abstract::The ultimate goal of bacterial based cancer therapy is to achieve non-toxic penetration and colonisation of the tumour microenvironment. To overcome this efficacy-limiting toxicity of anticancer immunotherapy, we have tested a therapy comprised of systemic delivery of a vascular disrupting agent to induce intratumoral...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186X.2020.1850739
更新日期:2020-12-21 00:00:00
abstract:BACKGROUND:The polycationic nanopeptide RKKRRQRRR is a fragment of HIV-Tat protein known to be a transfection enhancer. Such factors control the transfection efficiency of plasmid DNA complexed with tat peptide. However, their actions are poorly understood. METHODS AND RESULTS:Several cell lines and primary cells were...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/106118604200041403
更新日期:2004-01-01 00:00:00
abstract::We reside in an era of technological innovation and advancement despite which infectious diseases like malaria remain to be one of the greatest threats to the humans. Mortality rate caused by malaria disease is a huge concern in the twenty-first century. Multiple drug resistance and nonspecific drug targeting of the m...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.1080/1061186X.2017.1291645
更新日期:2017-08-01 00:00:00
abstract::Micro-needle arrays increase skin permeability by forming channels through the outer physical barrier, without stimulating pain receptors populating the underlying dermis. It was postulated that micro-needle arrays could facilitate transfer of DNA to human skin epidermis for cutaneous gene therapy applications. Platin...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860500383705
更新日期:2005-08-01 00:00:00
abstract::Lopinavir (LPV), an efficient drug for HIV infection treatment, was incorporated into biodegradable PLGA nanocapsules (NCs) embedded in microparticles (MCPs) using the spray-drying technique in an attempt to bypass the P-gp efflux and protect the drug from CYP3A pre-systemic metabolism without ritonavir (RTV). SEM obs...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186X.2018.1552275
更新日期:2019-01-01 00:00:00
abstract::Increased generation of reactive oxygen species (ROS) has been implicated in the pathogenesis of a variety of diseases such as cardiovascular diseases and cancer. NADPH oxidase (Nox), a multicomponent enzyme, has been identified as one of the key sources of ROS. Nox4, one of the seven members of Nox family (Nox1, Nox2...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.3109/1061186X.2015.1036276
更新日期:2015-12-01 00:00:00
abstract:BACKGROUND:Nanoparticles with controlled physical properties have been widely used for controlled release applications. In addition to shape, the anisotropic nature of the particles can be an important design criterion to ensure selective surface modification or independent release of combinations of drugs. PURPOSE:El...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2015.1076428
更新日期:2015-01-01 00:00:00
abstract::The aim of this work was to formulate minoxidil loaded liposome and niosome formulations to improve skin drug delivery. Multilamellar liposomes were prepared using soy phosphatidylcholine at different purity degrees (Phospholipon 90, 90% purity, soy lecithin (SL), 75% purity) and cholesterol (Chol), whereas niosomes w...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860600991993
更新日期:2007-02-01 00:00:00
abstract:OBJECTIVE:Histone deacetylases (HDACs) have been revealed to be involved in cerebrovascular diseases, while the role of HDAC9 in intracranial aneurysm (IA) remains seldom studied. We aim to explore the role of the HDAC9/microRNA-92a (miR-92a)/Bcl-2-like protein 11 (BCL2L11) axis in IA progression. METHODS:Expression o...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186X.2021.1878365
更新日期:2021-01-22 00:00:00
abstract::Drug systems targeting tumor cells using basic fibroblast growth factor (bFGF) have been widely reported. In this study, the peptide KRTGQYKLC (bFGFp), containing cysteine at the carboxyl termination of the bFGF-derived peptide, was applied as a novel ligand targeting tumor cells. bFGFp was conjugated with bovine seru...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860600849498
更新日期:2006-09-01 00:00:00
abstract::Recent studies have shown that drugs that are normally unable to cross the blood-brain barrier (BBB) following intravenous injection can be transported across this barrier by binding to poly(butyl cyanoacrylate) nanoparticles and coating with polysorbate 80. However, the mechanism of this transport so far was not know...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860290031877
更新日期:2002-06-01 00:00:00
abstract::Surface architecture of pharmaceutical nanocarriers (using polymeric micelles as an example) and the length of the spacer group through which specific ligand is attached to the carrier surface determine the interaction of ligand-bearing nanocarrier with cells. We have prepared surface-modified polyethyleneglycol-phosp...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860802230240
更新日期:2008-08-01 00:00:00
abstract::N-(2-hydroxypropyl)methacrylamide (HPMA) copolymers containing doxorubicin and galactosamine have been developed to target the hepatocyte galactose receptor with the aim of organ-specific chemotherapy of primary and metastatic liver disease. Previous biodistribution studies in rats and mice have used tyrosinamide inco...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869308996068
更新日期:1993-01-01 00:00:00