Abstract:
:Germ-free (GF) animals lack a colonic microflora like that seen in conventional (CV) animals. Bacterial presence plays a role in the development of glycoproteins in the gastrointestinal (GI) tract; the absence of a microbiota has been seen to suppress the production of certain glycoproteins and glycolipids. Binding patterns of lectins are modified when glycoprotein structures are altered (e.g., during development or disease). Little information on lectin binding patterns in mature GF animals is available. We examined the binding of free and N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer-conjugated fluorescein isothiocyanate (FITC)-labeled wheat germ agglutinin (WGA) [P(HPMA)-(WGA-FITC)] and FITC-labeled peanut agglutinin (PNA) [P(HPMA)-(PNA-FITC)] in CV and GF mouse colon with and without neuraminidase pretreatment. Anti-Thomsen-Friedenreich (TF) antigen (a development and disease-related glycoprotein) antibody binding was also examined in these tissues. Subtle differences were seen in the binding patterns between CV and GF animals. CV animals showed strong P(HPMA)-(WGA-FITC) binding in goblet cells, but minimal P(HPMA)-(PNA-FITC) binding was visible. In GF animals, luminal surface binding of P(HPMA)-(WGA-FITC) was visible, and goblet cell binding of P(HPMA)-(PNA-FITC) was seen. These subtle changes suggest that altered glycoprotein expression occurred under GF conditions.
journal_name
J Drug Targetjournal_title
Journal of drug targetingauthors
Wróblewski S,Ríhová B,Rossmann P,Hudcovicz T,Reháková Z,Kopecková P,Kopecek Jdoi
10.3109/10611860108997920keywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
85-94issue
2eissn
1061-186Xissn
1029-2330journal_volume
9pub_type
杂志文章abstract::The aim of this work was to formulate minoxidil loaded liposome and niosome formulations to improve skin drug delivery. Multilamellar liposomes were prepared using soy phosphatidylcholine at different purity degrees (Phospholipon 90, 90% purity, soy lecithin (SL), 75% purity) and cholesterol (Chol), whereas niosomes w...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860600991993
更新日期:2007-02-01 00:00:00
abstract::The human body has adapted to defend against the aggressive biological or chemical agents. As a result, the defence mechanisms of the human body became barriers for the drug delivery. Theoretically, any problem that prevents a drug from reaching its site of action is considered to be a barrier to drug delivery. The ai...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860903398099
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abstract:BACKGROUND:Treatment of late stage cancers has proven to be a very difficult task. Targeted therapy and combinatory drug administration may be the solution. PURPOSE:The study was performed to evaluate the therapeutic efficacy of PEG-PE micelles, co-loaded with curcumin (CUR) and doxorubicin (DOX), and targeted with an...
journal_title:Journal of drug targeting
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doi:10.3109/1061186X.2013.840639
更新日期:2013-12-01 00:00:00
abstract::A novel concept in drug delivery discussed here, takes advantage of certain properties of the drug "containers" cyclodextrins and liposomes to combine them into a single system thus circumventing problems associated with both systems. The concept, entailing entrapment of water-soluble cyclodextrin-drug inclusion compl...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.3109/10611869408996821
更新日期:1994-01-01 00:00:00
abstract::Liposomes have been prepared from dipalmitoylphosphatidylcholine (DPPC), cholesterol (Chol) and dimethyldioctadecylammonium bromide (DDAB). The cationic vesicles adsorb to biofilms of the skin-associated bacteria Staphylococcus epidermidis, which have a negative charge. Encapsulation of the antibacterial drug vancomyc...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869609015975
更新日期:1996-01-01 00:00:00
abstract::Recent studies have shown that drugs that are normally unable to cross the blood-brain barrier (BBB) following intravenous injection can be transported across this barrier by binding to poly(butyl cyanoacrylate) nanoparticles and coating with polysorbate 80. However, the mechanism of this transport so far was not know...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860290031877
更新日期:2002-06-01 00:00:00
abstract::Organic anion-transporting polypeptides (OATPs) encoded by the SLCO genes constitute an important transporter superfamily that mediates transmembrane transport of various clinical drugs and endogenous nutrients. Eleven human OATPs with different transport functions are expressed in various tissues. Bile acids, steroid...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.3109/1061186X.2013.832767
更新日期:2014-01-01 00:00:00
abstract::beta-Glucuronidase (GUSB) is a lysosomal enzyme that could be developed as a brain therapy for Type VII Mucopolysaccharidosis. However, GUSB does not cross the blood-brain barrier (BBB). To enable BBB transport of the enzyme, human GUSB was re-engineered as a fusion protein with the chimeric monoclonal antibody (MAb) ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860903353362
更新日期:2010-04-01 00:00:00
abstract::Improved delivery of macromolecular drugs to solid tumor is known as the enhanced permeability and retention (EPR) effect of macromolecular drugs and lipids. We report here that a prostaglandin I2 (PGI2) analogue induces enhancement of tumor-selective drug delivery, while it decreases tumor blood flow, in a rat tumor ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186031000086072
更新日期:2003-01-01 00:00:00
abstract::Doxorubicin (DXR) was encapsulated in long-circulating liposomes, composed of ganglioside GM1 (GM1)/distearoylphosphatidylcholine (DSPC)/cholesterol (CH) (0.13:1:1 in molar ratio) and sized to approximately 100 nm in mean diameter, with 98% entrapping efficiency by the transmembrane pH gradient method. Free DXR, DXR-D...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869308996086
更新日期:1993-01-01 00:00:00
abstract::The nerve growth factor (NGF) is essential for the survival of both peripheral ganglion cells and central cholinergic neurons in the basal forebrain. The accelerated loss of central cholinergic neurons during Alzheimer's disease may be a determinant cause of dementia, and this observation may suggest a possible therap...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860903112842
更新日期:2009-09-01 00:00:00
abstract::A polymeric gene carrier was developed to deliver vascular endothelial growth factor (VEGF) small interfering RNA (siRNA) for prostate cancer cells in a target-specific manner. Prostate cancer-binding peptide (PCP) was conjugated with polyethylenimine (PEI) via a poly(ethylene glycol) (PEG) linker (PEI-PEG-PCP). The P...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860902767232
更新日期:2009-05-01 00:00:00
abstract::The high-molecular-weight soluble aggregates of Fab fragments of murine antibodies against cardiac myosin were prepared as a potential long-circulating and low immunogenic pharmaceutical carriers by conjugation of thiolated Fab and Fab modified with succinimidyl 3-(2-pyridyldithio)propionate. The clearance time and bi...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869808997880
更新日期:1998-01-01 00:00:00
abstract::Solid tumors such as breast cancer have historically provided many challenges to anti-cancer therapy. Therapeutic hurdles to drug penetration in solid tumors include heterogeneous vascular supply and high interstitial pressures within tumor tissue, particularly in necrotic zones, lower pH and presence of leaky vascula...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.1080/10611860600809112
更新日期:2006-06-01 00:00:00
abstract::Supramolecules since ages have been characterised as self-associating systems emanating the properties directly related to the mode of molecular association. Similar to supramolecules, liquid crystals may also be considered as an associated system. The liquid crystals are defined as the state of matter with the charac...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869908996839
更新日期:1999-01-01 00:00:00
abstract::Highly lipophilic antitumor agent, palmitoyl rhizoxin (RS-1541), was incorporated into stable lipid emulsions about 100-1000nm in mean diameter consisting of triglyceride ODO and surfactant HCO-60. The pharmacokinetics of RS-1541 were studied after i.v. injection in mice, rats, rabbits, and dogs. Dog showed characteri...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869808997875
更新日期:1998-01-01 00:00:00
abstract::Despite the advantages of drug delivery through skin, transdermal drug delivery is only used with a small subset of drugs because most compounds cannot cross the skin at therapeutically useful rates. Recently, a new concept known as microneedle was introduced and could be used to pierce effectively to deliver drugs us...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860903115274
更新日期:2010-01-01 00:00:00
abstract::To design a novel cytospecific photosensitizer for photodynamic antitumor therapy, a fullerene (C(60)) was chemically modified with pullulan which is a water-soluble polysaccharide with a high affinity for asialoglycoprotein receptors. Ethylene diamine was introduced to the terminal aldehyde groups of pullulan by the ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611861003599479
更新日期:2010-09-01 00:00:00
abstract::The transport of 125I-radiolabelled latex nanoparticles across the nasal mucosa of rats was studied using a range of particle sizes and surface coatings. Translocation of the particles into the blood stream was examined by means of monitoring the radiolabel associated with the particles. Particles were detected in the...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860108997935
更新日期:2001-01-01 00:00:00
abstract::Human epidermal growth factor receptor 2 (HER2) expression has been shown to be increased in several types of human tumours. In this study, for the imaging of HER2-related tumours, a modified RNA aptamer with HER2-specific targeting was labelled with (99m)Tc, by using hydrazino nicotinamide (HYNIC) as the chelator in ...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2013.839688
更新日期:2014-02-01 00:00:00
abstract::Radiolabelled poly(methylmethacrylate) (PMMA) nanoparticles were coated with rat serum albumin (RSA), serum and inactivated serum, to examine the influence of these blood components on the body distribution of a model colloidal drug carrier. The particles were incubated overnight at 37 degrees C either in a 1% solutio...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869308998760
更新日期:1993-01-01 00:00:00
abstract::Therapeutic peptides are conventionally administered via subcutaneous injection. Chitosan-based nanoparticles are gaining increased attention for their ability to serve as a carrier for oral delivery of peptides and vaccination. They offered superior biocompatibiltiy, controlled drug release profile and facilitated ga...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
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更新日期:2018-08-01 00:00:00
abstract::A promising strategy to carry genetic material to brain cells either in vitro or in vivo is using the LDL receptor (LDLr) on blood-brain barrier. LDLr naturally help to low density lipoproteins (LDL(S)) transporting across the BBB by endocytosis. Here we present the idea of using the LDLr-mediated pathway for transpor...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860601148908
更新日期:2007-04-01 00:00:00
abstract::An aspargine-glycine-arginine (NGR) peptide modified single-walled carbon nanotubes (SWCNTs) system, developed by a simple non-covalent approach, could be loaded with the anticancer drug doxorubicin (DOX) and magnetic resonance imaging (MRI) contrast agent gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA). This...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186X.2016.1221958
更新日期:2017-02-01 00:00:00
abstract:BACKGROUND:Nanoparticles with controlled physical properties have been widely used for controlled release applications. In addition to shape, the anisotropic nature of the particles can be an important design criterion to ensure selective surface modification or independent release of combinations of drugs. PURPOSE:El...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2015.1076428
更新日期:2015-01-01 00:00:00
abstract::Most therapeutic agents are delivered orally. Consequently, the major classes of therapeutically useful chemicals are partially lipophilic, small molecular weight compounds. They have reasonable permeability coefficient values across cell membranes, including those of intestinal epithelia and vascular endothelia. In c...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.1080/10611860410001693715
更新日期:2004-02-01 00:00:00
abstract::The purpose of this research is to develop ligand-targeted plasmid based gene delivery systems for gene transfer to tumor endothelium. Cell adhesion assays were used to test the peptide inhibition of human endothelial cell adsorption to vitronectin-treated tissue culture plates. A series of RGD containing peptides wer...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860410001724468
更新日期:2004-05-01 00:00:00
abstract::Insulin and dexamethasone were encapsulated in poly(lactic-co-glycolic acid) (PLGA) microspheres to induce adipogenesis for potential applications in soft tissue reconstruction. Release kinetics and bioactivity of the drugs were examined. Surface morphology and diameter of the PLGA microspheres was evaluated using sca...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860802669231
更新日期:2009-04-01 00:00:00
abstract::It is well established that overproduction and accumulation of the β-amyloid (Aβ) peptide 1-42 (Aβ(1-42)) is a trigger of the pathological cascade in Alzheimer's disease (AD) that manifests as cognitive impairment. Ginsenoside Rg3 is an important constituent of ginseng, plays an essential role in memory and improved c...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186X.2017.1354002
更新日期:2018-02-01 00:00:00
abstract::Recently, considerable interest has been focussed on the use of biodegradable polymers for specialized applications such as controlled release of drug formulations; meanwhile, microsphere drug delivery systems using various kinds of biodegradable polymers have been studied extensively during the past two decades. In t...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860400010630
更新日期:2004-01-01 00:00:00