Potentiation of pro-inflammatory cytokine suppression and survival by microencapsulated dexamethasone in the treatment of experimental sepsis.

Abstract:

:Cytokine inhibiting drugs are much more effective when delivered intracellularly to phagocytic cells in the microencapsulated form. Dexamethasone is a powerful inhibitor of TNF-α cytokine through inhibition of NF-κB which is a gene regulator of multiple pro-inflammatory cytokines. We have determined the effect of microencapsulated dexamethasone in pro-inflammatory cytokine release both in in vitro using whole blood model, and in vivo using peritonitis model of septic shock. Microspheres of 1-4 μm mean size were prepared by using albumin polymer matrix in a one-step spray drying method. Microencapsulated form of dexamethasone with concentration of 10(-1), 10(-2) and 10(-3) M was compared to an equivalent concentration of solution form of dexamethasone in the in vitro whole blood model. The results show microencapsulated dexamethasone inhibited tumor necrosis factor-alpha (TNF-α) and interleukin-beta (IL-1β) significantly in comparison with the solution form of dexamethasone. The in vivo peritonitis model also demonstrated significant inhibition of TNF-α and IL-1β cytokines in microencapsulated form in comparison with solution form of dexamethasone. In the in vivo study, the animal survival rate after 5 days was 90%, dexamethasone in solution with gentamicin was 40% and gentamicin alone was 30%. This study demonstrates significantly improved inhibition of TNF-α and IL-1β both in vivo and in vitro when dexamethasone was used in microencapsulated form.

journal_name

J Drug Target

authors

Uddin MN,Siddiq A,Oettinger CW,D'Souza MJ

doi

10.3109/1061186X.2011.561856

subject

Has Abstract

pub_date

2011-11-01 00:00:00

pages

752-60

issue

9

eissn

1061-186X

issn

1029-2330

journal_volume

19

pub_type

杂志文章
  • Secure and effective gene delivery system of plasmid DNA coated by polynucleotide.

    abstract::Polynucleotides are anionic macromolecules which are expected to transfer into the targeted cells through specific uptake mechanisms. So, we developed polynucleotides coating complexes of plasmid DNA (pDNA) and polyethylenimine (PEI) for a secure and efficient gene delivery system and evaluated their usefulness. Polya...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.3109/1061186X.2014.950665

    authors: Kodama Y,Ohkubo C,Kurosaki T,Egashira K,Sato K,Fumoto S,Nishida K,Higuchi N,Kitahara T,Nakamura T,Sasaki H

    更新日期:2015-01-01 00:00:00

  • Non-covalent ligand conjugation to biotinylated DNA nanoparticles using TAT peptide genetically fused to monovalent streptavidin.

    abstract::DNA nanoparticles (DNA NPs), which self-assemble from DNA plasmids and poly-L-lysine (pLL)-polyethylene glycol (PEG) block copolymers, transfect several cell types in vitro and in vivo with minimal toxicity and immune response. To further enhance the gene transfer efficiency of DNA NP and control its tropism, we estab...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.3109/1061186X.2012.712128

    authors: Sun W,Fletcher D,van Heeckeren RC,Davis PB

    更新日期:2012-09-01 00:00:00

  • The human Nox4: gene, structure, physiological function and pathological significance.

    abstract::Increased generation of reactive oxygen species (ROS) has been implicated in the pathogenesis of a variety of diseases such as cardiovascular diseases and cancer. NADPH oxidase (Nox), a multicomponent enzyme, has been identified as one of the key sources of ROS. Nox4, one of the seven members of Nox family (Nox1, Nox2...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章,评审

    doi:10.3109/1061186X.2015.1036276

    authors: Guo S,Chen X

    更新日期:2015-12-01 00:00:00

  • Effects of skeleton structure on necrosis targeting and clearance properties of radioiodinated dianthrones.

    abstract::Necrosis avid agents (NAAs) can be used for diagnose of necrosis-related diseases, evaluation of therapeutic responses and targeted therapeutics of tumor. In order to probe into the effects of molecular skeleton structure on necrosis targeting and clearance properties of radioiodinated dianthrones, four dianthrone com...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.3109/1061186X.2015.1113541

    authors: Zhang D,Jiang C,Yang S,Gao M,Huang D,Wang X,Shao H,Feng Y,Sun Z,Ni Y,Zhang J,Yin Z

    更新日期:2016-01-01 00:00:00

  • Gold nanoparticles potentiates N-acetylcysteine effects on neurochemicals alterations in rats after polymicrobial sepsis.

    abstract::Herein, we report the effect of gold nanoparticles (AuNP) and n-acetylcysteine (NAC) isolated or in association as important anti-inflammatory and antioxidant compounds on brain dysfunction in septic rats. Male Wistar rats after sham operation or caecal ligation and perforation (CLP) were treated with subcutaneously i...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.1080/1061186X.2019.1678168

    authors: Petronilho F,Tenfen L,Della Giustina A,Joaquim L,Novochadlo M,de Oliveira Junior AN,Bagio E,Goldim MPS,de Carli RJ,Bonfante SRSA,Metzker KLL,Muttini S,Dos Santos TM,de Oliveira MP,Engel NA,Rezin GT,Kanis LA,Barichello T

    更新日期:2020-04-01 00:00:00

  • Strategies for hepatic gene correction.

    abstract::Gene augmentation has been the paradigm in the majority of gene therapy protocols but in recent years the potential of repairing the mutated gene in situ by targeted gene correction has become a reality. In fact, targeted gene repair has many advantages over conventional replacement strategies, notably the possibility...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章,评审

    doi:10.1080/10611860290016739

    authors: Richardson P,Thoma C,Kren BT,Steer CJ

    更新日期:2002-03-01 00:00:00

  • A gene delivery approach for antimicrobials: expression of defensins.

    abstract:INTRODUCTION:Peptide antibiotics as new therapeutic agents are becoming a popular option to investigate due to their broad bacterial target selectivity and limited resistance problems. Although attractive, these new drug candidates have several limitations including low potency and delivery issues which face all peptid...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.1080/10611860600907767

    authors: Zhang C,Yadava P,Sun J,Hughes JA

    更新日期:2006-11-01 00:00:00

  • Internalization and subcellular fate of aptamer and peptide dual-functioned nanoparticles.

    abstract:PURPOSE:To evaluate the internalization and subcellular fate of AS1411 aptamer (for glioma targeting) and TGN peptide (for blood-brain barrier targeting)-modified nanoparticles (AsTNPs), which was important for optimizing targeted delivery systems and realizing the potential toxicity to cells. METHODS:Organelles were ...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.3109/1061186X.2014.886038

    authors: Gao H,Yang Z,Zhang S,Pang Z,Jiang X

    更新日期:2014-06-01 00:00:00

  • Similar efficiency of DNA-liposome complexes and retrovirus-producing cells for HSV-tk suicide gene therapy of peritoneal carcinomatosis.

    abstract::Several experimental approaches have been tested for suicide gene delivery into tumor cells, including viral and non-viral vectors. In this study, we compared the efficiency of Herpes Simplex Virus type 1 thymidine kinase gene (HSV-tk) delivery by retrovirus-producing cells and DNA/liposome complexes for the treatment...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.3109/10611860008996854

    authors: Princen F,Lechanteur C,Lopez M,Gielen J,Bours V,Merville MP

    更新日期:2000-01-01 00:00:00

  • α-Tocopherol as functional excipient for resveratrol and coenzyme Q10-loaded SNEDDS for improved bioavailability and prophylaxis of breast cancer.

    abstract::The present study evaluates the prophylactic efficacy of α-tocopherol (α-TOH), resveratrol (RES), and coenzyme Q10 (CoQ10) co-loaded self-nanoemulsifying drug delivery system (α-TOH-RES-CoQ10 SNEDDS) in 7,12-Dimethylbenz[a]anthracene (DMBA) induced breast cancer model. SNEDDS formulation components were rationally sel...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.1080/1061186X.2017.1298603

    authors: Jain S,Garg T,Kushwah V,Thanki K,Agrawal AK,Dora CP

    更新日期:2017-07-01 00:00:00

  • Neuroprotective effects of a biodegradable poly(lactic-co-glycolic acid)-ginsenoside Rg3 nanoformulation: a potential nanotherapy for Alzheimer's disease?

    abstract::It is well established that overproduction and accumulation of the β-amyloid (Aβ) peptide 1-42 (Aβ(1-42)) is a trigger of the pathological cascade in Alzheimer's disease (AD) that manifests as cognitive impairment. Ginsenoside Rg3 is an important constituent of ginseng, plays an essential role in memory and improved c...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.1080/1061186X.2017.1354002

    authors: Aalinkeel R,Kutscher HL,Singh A,Cwiklinski K,Khechen N,Schwartz SA,Prasad PN,Mahajan SD

    更新日期:2018-02-01 00:00:00

  • Basic fibroblast growth factor-binding peptide as a novel targeting ligand of drug carrier to tumor cells.

    abstract::Drug systems targeting tumor cells using basic fibroblast growth factor (bFGF) have been widely reported. In this study, the peptide KRTGQYKLC (bFGFp), containing cysteine at the carboxyl termination of the bFGF-derived peptide, was applied as a novel ligand targeting tumor cells. bFGFp was conjugated with bovine seru...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.1080/10611860600849498

    authors: Terada T,Mizobata M,Kawakami S,Yabe Y,Yamashita F,Hashida M

    更新日期:2006-09-01 00:00:00

  • Styrene-maleic acid-copolymer conjugated zinc protoporphyrin as a candidate drug for tumor-targeted therapy and imaging.

    abstract::Previous studies indicated the potential of zinc protoporphyrin (ZnPP) as an antitumor agent targeting to the tumor survival factor heme oxygenase-1, and/or for photodynamic therapy (PDT). In this study, to achieve tumor-targeted delivery, styrene-maleic acid-copolymer conjugated ZnPP (SMA-ZnPP) was synthesized via am...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.3109/1061186X.2015.1077851

    authors: Fang J,Tsukigawa K,Liao L,Yin H,Eguchi K,Maeda H

    更新日期:2016-01-01 00:00:00

  • Silica-deposited phospholipid nanotubules as a plausible drug targeting system.

    abstract::An aqueous dispersion of self-organized phospholipid tubules has been utilized as the template for silica-deposited nanotubules (approximately 0.5 microm thick and >10 microm long) by a sol-gel method. The formation of the hybrid tubules was mechanistically investigated by controlled sol-gel reaction. The incorporatio...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.1080/10611860802295581

    authors: Kim I,Park YH,Rey DA,Batt CA

    更新日期:2008-11-01 00:00:00

  • Biphasic interactions between a cationic dendrimer and actin.

    abstract::Gene delivery systems face the problem not only of the route toward the cell and tissues in question, but also of the molecularly crowded environment of both the cytoplasm and the nucleus itself. One of the physical barriers in the cytoplasm for diffusing nanoparticles is an actin network. Here, we describe the findin...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.3109/1061186X.2010.521159

    authors: Ruenraroengsak P,Florence AT

    更新日期:2010-12-01 00:00:00

  • Low-intensity light-induced paclitaxel release from lipid-based nano-delivery systems.

    abstract::Light-induced drug release has been explored as a strategy for externally modulating the release of drug from delivery systems. This study reports the development of a solid lipid nanoparticulate system (SLN) for paclitaxel (PTX), where photosensitizer-mediated oxidation of lipids was used as a mechanism for controlli...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.1080/1061186X.2019.1571066

    authors: Meerovich I,Nichols MG,Dash AK

    更新日期:2019-11-01 00:00:00

  • Influence of particle size on drug delivery to rat alveolar macrophages following pulmonary administration of ciprofloxacin incorporated into liposomes.

    abstract::In order to confirm the efficacy of ciprofloxacin (CPFX) incorporated into liposomes (CPFX-liposomes) for treatment of respiratory intracellular parasite infections, the influence of particle size on drug delivery to rat alveolar macrophages (AMs) following pulmonary administration of CPFX-liposomes was investigated. ...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.1080/10611860600834375

    authors: Chono S,Tanino T,Seki T,Morimoto K

    更新日期:2006-09-01 00:00:00

  • Peptide-mediated gene transfer of cationic lipid/plasmid DNA complexes to endothelial cells.

    abstract::The purpose of this research is to develop ligand-targeted plasmid based gene delivery systems for gene transfer to tumor endothelium. Cell adhesion assays were used to test the peptide inhibition of human endothelial cell adsorption to vitronectin-treated tissue culture plates. A series of RGD containing peptides wer...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.1080/10611860410001724468

    authors: Anwer K,Kao G,Rolland A,Driessen WH,Sullivan SM

    更新日期:2004-05-01 00:00:00

  • Colon-specific delivery and enhanced colonic absorption of [Asu(1,7)]-eel calcitonin using chitosan capsules containing various additives in rats.

    abstract::The objective of this study was to estimate the colon-specific delivery of [Asu1,7]-eel calcitonin (ECT) using chitosan capsules in rats. The intestinal absorption of ECT was evaluated by measuring the plasma calcium levels after oral administration of the chitosan capsules containing ECT and different combinations of...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.1080/10611860600648494

    authors: Fetih G,Fausia H,Okada N,Fujita T,Attia M,Yamamoto A

    更新日期:2006-04-01 00:00:00

  • Recent advances in metal nanoparticles in cancer therapy.

    abstract::Metal nanoparticles (NPs) may have the potential to overcome problems related to conventional chemotherapy. Metal NPs reported to play a beneficial and powerful role in cancer therapy providing better targeting, gene silencing and drug delivery. Functionalised metal NPs with targeting ligands offer a better control of...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.1080/1061186X.2017.1400553

    authors: Sharma A,Goyal AK,Rath G

    更新日期:2018-09-01 00:00:00

  • The potential use of rabies virus glycoprotein-derived peptides to facilitate drug delivery into the central nervous system: a mini review.

    abstract::Rabies virus glycoprotein (RVG), a 505 amino acid type-1 glycoprotein, is responsible for the neurotrophic nature of the rabies virus infection. Despite varying reports in the literature as to which receptor is ultimately responsible for interaction of RVG with the nervous system, there is a strong argument for major ...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章,评审

    doi:10.1080/1061186X.2016.1223676

    authors: Huey R,Hawthorne S,McCarron P

    更新日期:2017-06-01 00:00:00

  • Octreotide-conjugated PAMAM for targeted delivery to somatostatin receptors over-expressed tumor cells.

    abstract:PURPOSE:An octreotide-conjugated polyamidoamine (PAMAM) dendrimer was synthesized and employed as nanocarriers of methotrexate (MTX), for targeting to the somatostatin receptors over-expressed tumor cells. METHODS:PAMAM-PEG-octreotide (PPO) and PAMAM-PEG (PPG) were synthesized and characterized. The cellular uptake of...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.3109/1061186X.2013.879386

    authors: Peng J,Qi X,Chen Y,Ma N,Zhang Z,Xing J,Zhu X,Li Z,Wu Z

    更新日期:2014-06-01 00:00:00

  • Sensitized liposomes as an antigen delivery system for the stimulation of mucosal immunity.

    abstract::This study was designed to exploit the ability of Peyer's patch M cells to recognize antigen-antibody complexes in the targeted delivery of a model antigen for the induction of mucosal immunity. Sensitized liposomes consisted of an entrapped model antigen, ovalbumin (OVA), and coated with unrelated antigen-antibody co...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.3109/10611869708995854

    authors: Velez CN,Tonkonogy SL,Lichtman SN,Cho MJ

    更新日期:1997-01-01 00:00:00

  • Oridonin-loaded poly(epsilon-caprolactone)-poly(ethylene oxide)-poly(epsilon-caprolactone) copolymer nanoparticles: preparation, characterization, and antitumor activity on mice with transplanted hepatoma.

    abstract::The aim of the present study was to develop a polymeric delivery system for water-insoluble drug oridonin. Amphiphilic block copolymers, poly(epsilon-caprolactone)-poly(ethylene glycol)-poly (epsilon-caprolactone) (PCL-PEO-PCL), were synthesized by ring-opening polymerization of caprolactone initiated by the hydroxyl ...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.1080/10611860802200938

    authors: Feng N,Wu P,Li Q,Mei Y,Shi S,Yu J,Xu J,Liu Y,Wang Y

    更新日期:2008-07-01 00:00:00

  • A new method to isolate polyalkylcyanoacrylate nanoparticle preparations.

    abstract::A simple method for the separation of polyalkylcyanoacrylate nanoparticles was developed using polyisohexylcyanoacrylate (PIHCA) as a model. Fluorescein isothiocyanate dextran 70 was used to label the nanoparticles. Ultracentrifugation onto a performed sucrose gradient allowed the easy elimination of the dextran and o...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.3109/10611869509059216

    authors: Pinto-Alphandary H,Balland O,Couvreur P

    更新日期:1995-01-01 00:00:00

  • Interaction of serum components with poly(methylmethacrylate) nanoparticles and the resulting body distribution after intravenous injection in rats.

    abstract::Radiolabelled poly(methylmethacrylate) (PMMA) nanoparticles were coated with rat serum albumin (RSA), serum and inactivated serum, to examine the influence of these blood components on the body distribution of a model colloidal drug carrier. The particles were incubated overnight at 37 degrees C either in a 1% solutio...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.3109/10611869308998760

    authors: Borchard G,Kreuter J

    更新日期:1993-01-01 00:00:00

  • Transport of nanoparticles across the rat nasal mucosa.

    abstract::The transport of 125I-radiolabelled latex nanoparticles across the nasal mucosa of rats was studied using a range of particle sizes and surface coatings. Translocation of the particles into the blood stream was examined by means of monitoring the radiolabel associated with the particles. Particles were detected in the...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.3109/10611860108997935

    authors: Brooking J,Davis SS,Illum L

    更新日期:2001-01-01 00:00:00

  • Enhanced gene delivery using Bubble liposomes and ultrasound for folate-PEG liposomes.

    abstract::We have previously reported that the transfection efficiency of laminin-derived AG73-peptide labeled polyethyleneglycol-modified liposomes (AG73-PEG liposomes) was enhanced by echo-contrast gas entrapping PEG liposomes (Bubble liposomes, BLs) and ultrasound (US) exposure by improving endosomal escape. However, it has ...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.3109/1061186X.2012.660162

    authors: Omata D,Negishi Y,Hagiwara S,Yamamura S,Endo-Takahashi Y,Suzuki R,Maruyama K,Aramaki Y

    更新日期:2012-05-01 00:00:00

  • Brain-targeted delivery of paclitaxel using glutathione-coated nanoparticles for brain cancers.

    abstract::Paclitaxel is not effective for treatment of brain cancers because it cannot cross the blood-brain barrier (BBB) due to efflux by P-glycoprotein (P-gp). In this work, glutathione-coated poly-(lactide-co-glycolide) (PLGA) nanoparticles (NPs) of paclitaxel were developed for brain targeting for treatment of brain cancer...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.3109/1061186X.2011.589435

    authors: Geldenhuys W,Mbimba T,Bui T,Harrison K,Sutariya V

    更新日期:2011-11-01 00:00:00

  • New terpolymers as hydrogels for oral protein delivery application.

    abstract::The purpose of this study is to develop novel intestinal-specific insulin delivery systems with pH-sensitive swelling and drug release properties. The glucose-6-acrylate-1,2,3,4-tetraacetate (GATA) monomer was prepared under mild conditions. Cubane-1,4-dicarboxylic acid linked to two 2-hydroxyethyl methacrylate groups...

    journal_title:Journal of drug targeting

    pub_type: 杂志文章

    doi:10.1080/10611860802438728

    authors: Mahkam M

    更新日期:2009-01-01 00:00:00