Abstract：
BACKGROUND:The polycationic nanopeptide RKKRRQRRR is a fragment of HIV-Tat protein known to be a transfection enhancer. Such factors control the transfection efficiency of plasmid DNA complexed with tat peptide. However, their actions are poorly understood. METHODS AND RESULTS:Several cell lines and primary cells were used in transfection experiments. The optimal charge ratio of plasmid to the branched 8Tat peptide was 1:8 for all cell lines and primary cells tested except primary human skin fibroblasts where it was 1:4. The conditions of complex formation (volume, DNA-peptide-solution mixing order and the nature of solution (HEPES, 0.15 M NaCl or 5% dextrose)) influenced transfection efficiency. Chloroquine increased the transgene expression 50-fold in HeLa cells but had little impact on Cos7 cells or 3T3 fibroblasts. The integrity of plasmid DNA in the presence of DNase I was shown to be dependent on the DNA/Tat ratio and the nature of the complex solvent. Complexes with -/+ charge ratio of 1:8 formulated in NaCl and dextrose provided high degree of DNA resistance towards nuclease degradation. CONCLUSIONS:The conditions of DNA-peptide complex formation and DNA/Tat ratio have significant impact on the level of transgene expression and degree of DNA protection from nuclease attack. The level of elevation of gene delivery in the presence of chloroquine varied considerably between different cells, indicating possible different mechanisms of plasmid-peptide internalisation and intracellular trafficking. The efficiency of branched 8Tat peptide as a plasmid DNA carrier varied between different cell types and have to be optimised for each application.
journal_name
J Drug Targetjournal_title
Journal of drug targetingauthors
Hellgren I,Gorman J,Sylvén Cdoi
10.1080/106118604200041403keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
39-47issue
1eissn
1061-186Xissn
1029-2330pii
3PNR6Q39PA4EUBVNjournal_volume
12pub_type
杂志文章abstract：:The ability of a polymer therapeutic to access the appropriate subcellular location is crucial to its efficacy and is defined to a large part by the many and complex cellular biological and biochemical barriers such that a construct must traverse. It is shown here that model dextrin conjugates are able to pass through...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.1080/1061186X.2017.1378660
更新日期：2017-01-01 00:00:00
abstract：:The aim of this work was to formulate minoxidil loaded liposome and niosome formulations to improve skin drug delivery. Multilamellar liposomes were prepared using soy phosphatidylcholine at different purity degrees (Phospholipon 90, 90% purity, soy lecithin (SL), 75% purity) and cholesterol (Chol), whereas niosomes w...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.1080/10611860600991993
更新日期：2007-02-01 00:00:00
abstract：:Cancer is one of the most common diseases all over the world; many people suffer from diverse types of cancer. However, currently there is no exact cure or therapy developed for cancer. On the other hand, nanoparticles are defined as microscopic particles that have dimensions less than 100 nm and they are known for th...
journal_title：Journal of drug targeting
pub_type： 杂志文章,评审
doi：10.1080/1061186X.2018.1527338
更新日期：2019-08-01 00:00:00
abstract：:Recently, considerable interest has been focussed on the use of biodegradable polymers for specialized applications such as controlled release of drug formulations; meanwhile, microsphere drug delivery systems using various kinds of biodegradable polymers have been studied extensively during the past two decades. In t...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.1080/10611860400010630
更新日期：2004-01-01 00:00:00
abstract：:Paclitaxel is not effective for treatment of brain cancers because it cannot cross the blood-brain barrier (BBB) due to efflux by P-glycoprotein (P-gp). In this work, glutathione-coated poly-(lactide-co-glycolide) (PLGA) nanoparticles (NPs) of paclitaxel were developed for brain targeting for treatment of brain cancer...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.3109/1061186X.2011.589435
更新日期：2011-11-01 00:00:00
abstract：:Gene delivery systems face the problem not only of the route toward the cell and tissues in question, but also of the molecularly crowded environment of both the cytoplasm and the nucleus itself. One of the physical barriers in the cytoplasm for diffusing nanoparticles is an actin network. Here, we describe the findin...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.3109/1061186X.2010.521159
更新日期：2010-12-01 00:00:00
abstract：:Toxoplasmic encephalitis (TE) is the most common clinical manifestation of reactivated infection with Toxoplasma gondii in immunocompromised patients that is lethal if untreated. The combination of pyrimethamine plus sulfadiazine or clindamycin is the standard therapy for the treatment of TE, but these combinations ar...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.3109/10611861003733995
更新日期：2011-02-01 00:00:00
abstract：:The biodistribution of orally and intravenously administered archaeosomes in mice was compared to that of archaeosomes containing either coenzyme Q10 (archaeosome-CoQ10), polyethylene glycol (archaeosome-PEG), or PEG plus CoQ10 (archaeosome-PEG-CoQ10). The archaeosome formulations were prepared by a reverse-phase evap...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.3109/10611869909085521
更新日期：2000-01-01 00:00:00
abstract：:Interactions between phosphatidylcholine (PC) or phosphatidylserine (PS) liposomes and human umbilical vein endothelial cells (HUVEC) or human promyelocytic leukemia cells (HL60) were investigated. Pyramine encapsulating or rhodamine incorporating small unilamellar liposomes with mean diameters around 80 nm (demonstra...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.3109/10611860008997910
更新日期：2000-01-01 00:00:00
abstract：:In this review, we emphasize the efforts on the development of radiolabeled nanoparticles (NPs) for cancer treatment, i.e. theranostic tools based on nanotechnology and nuclear medicine. Currently, radionuclide therapy remains to be an important treatment option. The ionizing radiation from radionuclides (not provided...
journal_title：Journal of drug targeting
pub_type： 杂志文章,评审
doi：10.3109/1061186X.2014.988216
更新日期：2015-04-01 00:00:00
abstract：:Graphene, the mother of all carbon materials, has unlocked a new era of biomedical nanomaterials due to its exceptional biocompatibility, physicochemical and mechanical properties. It is a single atom thick, nanosized, two-dimensional structure and provides high surface area with adjustable surface chemistry to form h...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.1080/1061186X.2018.1437920
更新日期：2018-12-01 00:00:00
abstract：:Tumour-associated macrophages (TAMs) represent as much as 50% of the solid mass in different types of human solid tumours including lung, breast, ovarian and pancreatic adenocarcinomas. The tumour microenvironment (TME) plays an important role in the polarisation of macrophages into the M1 phenotype, which is tumour-s...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.1080/1061186X.2019.1648478
更新日期：2020-02-01 00:00:00
abstract：:The aim of this study was to evaluate the pharmacokinetics (PK), tissue distribution, and the specific drug targeting of cefpiramide sodium-loaded liposomes (CPMS-Lips) compared with cefpiramide sodium solution (CPMS-Sol) in mice. CPMS-Lips were prepared by reverse phase evaporation method. In the PK and biodistributi...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.3109/10611861003667607
更新日期：2011-01-01 00:00:00
abstract：:Nanoparticles have shown great promise in the treatment of cancer, with a demonstrated potential in targeted drug delivery. Among a myriad of nanocarriers that have been recently developed, dendrimers have attracted a great deal of scientific interests due to their unique chemical and structural properties that allow ...
journal_title：Journal of drug targeting
pub_type： 杂志文章,评审
doi：10.3109/1061186X.2015.1052077
更新日期：2015-01-01 00:00:00
abstract：:Pathophysiological molecules in the extracellular environment offer excellent targets that can be exploited for designing drug targeting systems. Matrix metalloproteases (MMPs) are a family of extracellular proteolytic enzymes that are characterized by their overexpression or overactivity in several pathologies. Over ...
journal_title：Journal of drug targeting
pub_type： 杂志文章,评审
doi：10.1080/10611860600968967
更新日期：2007-01-01 00:00:00
abstract：OBJECTIVE:Histone deacetylases (HDACs) have been revealed to be involved in cerebrovascular diseases, while the role of HDAC9 in intracranial aneurysm (IA) remains seldom studied. We aim to explore the role of the HDAC9/microRNA-92a (miR-92a)/Bcl-2-like protein 11 (BCL2L11) axis in IA progression. METHODS:Expression o...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.1080/1061186X.2021.1878365
更新日期：2021-01-22 00:00:00
abstract：:Several experimental approaches have been tested for suicide gene delivery into tumor cells, including viral and non-viral vectors. In this study, we compared the efficiency of Herpes Simplex Virus type 1 thymidine kinase gene (HSV-tk) delivery by retrovirus-producing cells and DNA/liposome complexes for the treatment...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.3109/10611860008996854
更新日期：2000-01-01 00:00:00
abstract：:Rabies virus glycoprotein (RVG), a 505 amino acid type-1 glycoprotein, is responsible for the neurotrophic nature of the rabies virus infection. Despite varying reports in the literature as to which receptor is ultimately responsible for interaction of RVG with the nervous system, there is a strong argument for major ...
journal_title：Journal of drug targeting
pub_type： 杂志文章,评审
doi：10.1080/1061186X.2016.1223676
更新日期：2017-06-01 00:00:00
abstract：:Cytokine inhibiting drugs are much more effective when delivered intracellularly to phagocytic cells in the microencapsulated form. Dexamethasone is a powerful inhibitor of TNF-α cytokine through inhibition of NF-κB which is a gene regulator of multiple pro-inflammatory cytokines. We have determined the effect of micr...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.3109/1061186X.2011.561856
更新日期：2011-11-01 00:00:00
abstract：:A simple method for the separation of polyalkylcyanoacrylate nanoparticles was developed using polyisohexylcyanoacrylate (PIHCA) as a model. Fluorescein isothiocyanate dextran 70 was used to label the nanoparticles. Ultracentrifugation onto a performed sucrose gradient allowed the easy elimination of the dextran and o...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.3109/10611869509059216
更新日期：1995-01-01 00:00:00
abstract：:Increased generation of reactive oxygen species (ROS) has been implicated in the pathogenesis of a variety of diseases such as cardiovascular diseases and cancer. NADPH oxidase (Nox), a multicomponent enzyme, has been identified as one of the key sources of ROS. Nox4, one of the seven members of Nox family (Nox1, Nox2...
journal_title：Journal of drug targeting
pub_type： 杂志文章,评审
doi：10.3109/1061186X.2015.1036276
更新日期：2015-12-01 00:00:00
abstract：:In this study, folate receptor (FR) targeted liposome microbubbles loaded with oridonin (ORI) (F-LMB-ORI), liposome loaded with ORI (L-ORI) and liposome microbubbles loaded with ORI (LMB-ORI) were prepared. In vitro release properties, cellular uptake and cytotoxicity in HepG-2 cells as well as in vivo antitumor effec...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.1080/1061186X.2016.1200588
更新日期：2017-01-01 00:00:00
abstract：:An aqueous dispersion of self-organized phospholipid tubules has been utilized as the template for silica-deposited nanotubules (approximately 0.5 microm thick and >10 microm long) by a sol-gel method. The formation of the hybrid tubules was mechanistically investigated by controlled sol-gel reaction. The incorporatio...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.1080/10611860802295581
更新日期：2008-11-01 00:00:00
abstract：:Surface architecture of pharmaceutical nanocarriers (using polymeric micelles as an example) and the length of the spacer group through which specific ligand is attached to the carrier surface determine the interaction of ligand-bearing nanocarrier with cells. We have prepared surface-modified polyethyleneglycol-phosp...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.1080/10611860802230240
更新日期：2008-08-01 00:00:00
abstract：:The effects of concentration, amperage and duration on the antidiuretic response induced by iontophoretic delivery of desmopressin acetate (DDAVP) were examined using a diabetes insipidus model in rats. A higher current density brought about a larger and longer antidiuretic response. Prolonged iontophoretic duration c...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.3109/10611869509015918
更新日期：1995-01-01 00:00:00
abstract：:Targeting of anti-tumor drugs to the urinary bladder for the treatment of bladder carcinoma may be useful, since these agents generally have a low degree of urinary excretion and are highly toxic elsewhere in the body. The anti-tumor drug doxorubicin was coupled to the low-molecular weight protein lysozyme via the aci...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.1080/10611860290007568
更新日期：2002-02-01 00:00:00
abstract：:Light-induced drug release has been explored as a strategy for externally modulating the release of drug from delivery systems. This study reports the development of a solid lipid nanoparticulate system (SLN) for paclitaxel (PTX), where photosensitizer-mediated oxidation of lipids was used as a mechanism for controlli...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.1080/1061186X.2019.1571066
更新日期：2019-11-01 00:00:00
abstract：:As a percutaneous sustained-release preparation, insulin micropiles (MPs) were prepared with biodegradable polymers poly(lactic acid) (PLA), poly(ϵ-caprolactone) (PCL) and poly(lactic-co-glycolic acid) (PLGA) as the base. The obtained PLA, PCL, and PLGA MPs of which the insulin:polymer ratio was 1:2 were administered ...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.3109/1061186X.2010.492521
更新日期：2011-04-01 00:00:00
abstract：:The aim of the present study was to develop a polymeric delivery system for water-insoluble drug oridonin. Amphiphilic block copolymers, poly(epsilon-caprolactone)-poly(ethylene glycol)-poly (epsilon-caprolactone) (PCL-PEO-PCL), were synthesized by ring-opening polymerization of caprolactone initiated by the hydroxyl ...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.1080/10611860802200938
更新日期：2008-07-01 00:00:00
abstract：:Highly lipophilic antitumor agent, palmitoyl rhizoxin (RS-1541), was incorporated into stable lipid emulsions about 100-1000nm in mean diameter consisting of triglyceride ODO and surfactant HCO-60. The pharmacokinetics of RS-1541 were studied after i.v. injection in mice, rats, rabbits, and dogs. Dog showed characteri...
journal_title：Journal of drug targeting
pub_type： 杂志文章
doi：10.3109/10611869808997875
更新日期：1998-01-01 00:00:00
