Abstract:
:This study was aimed to generate a new agent, norcantharidin (NCTD) encapsulated liposomes modified with a novel murine anti-human CD19 monoclonal antibody 2E8 (2E8–NCTD–liposomes), to specifically target the B-lineage leukemia stem cells (B-LSCs) and their progeny in vitro. Our results have shown that the positive percentage of 2E8–NCTD–liposomes on CD19+ Nalm-6 cells was (95.82 ± 1.09)%, significantly higher than that on CD19- Molt-3 cells [(2.94 ± 0.07)%, P<0.01], demonstrated by using multiparameter flow cytometry. The IC50 of 2E8–NCTD–liposomes on Nalm-6 cells using MTT assay was 14.52 µM, which was significantly lower than that on Molt-3 cells (45.89 µM, P < 0.01). The confocal microscopy and multiparameter flow cytometry analyses revealed that the internalization of 2E8–NCTD–liposomes into the cells and subsequently the release of NCTD into the cytoplasm to induce the apoptosis of B cells were responsible for specific cytotoxicity to the cells targeted. Real-time RT-PCR showed that the immunoliposomes were able to induce the apoptosis of B-LSCs via down-regulating the HLF and up-regulating the NFIL3 (nuclear factor, IL3 regulated) expressions at the mRNA level. Our conclusion is that 2E8–NCTD–liposome is a promising agent for selectively eradicating the B-LSCs and their progeny in vitro which warrants further studies in vivo.
journal_name
J Drug Targetjournal_title
Journal of drug targetingauthors
Zhang J,Tang Y,Li S,Liao C,Guo Xdoi
10.3109/10611861003649720subject
Has Abstractpub_date
2010-11-01 00:00:00pages
675-87issue
9eissn
1061-186Xissn
1029-2330journal_volume
18pub_type
杂志文章abstract::HEK cells were transfected with the GFP gene using various vectors: naked DNA, lipofectamine, and both PLGA and P(FASA) plasmid-loaded nanospheres. All methods were assessed alone and with the use of chloroquine, a lysosomal enzyme inhibitor. Transfection efficiencies were determined and compared at various times post...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186021000038373
更新日期:2002-09-01 00:00:00
abstract::Recent research into the complex and varied components of rheumatoid arthritis (RA) is leading to the development of more effective targets for pharmaceutical approach than even before. Current treatment of RA frequently includes the use of nonsteroidal anti-inflammatory drugs, such as Diclofenac sodium (DFNa) in spit...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860701725191
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Synthesized dl-Nordihydroguaiaretic acid (dl-NGDA or "Nordy") can inhibit the growth of malignant human tumors, especially the tumor angiogenesis. However, its liposoluble nature limits its in vivo efficacy in the hydrosoluble circulation of human. PURPOSE:We tried to use the ultrasonic microbubble as the c...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2016.1144058
更新日期:2016-09-01 00:00:00
abstract::siRNA therapeutics has developed rapidly and already there are clinical trials ongoing or planned; however, the delivery of siRNA into cells, tissues or organs remains to be a major obstacle. Lipid-based vectors hold the most promising position among non-viral vectors, as they have a similar structure to cell or organ...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.3109/1061186X.2012.719232
更新日期:2012-11-01 00:00:00
abstract::Glioma is one of the most commonly occurring malignant brain tumours which need proper treatment strategy. The current therapies for treating glioma like surgical resection, radiotherapy, and chemotherapy have failed in achieving satisfactory results and this forms a rationale for the development of novel drug deliver...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.3109/1061186X.2014.916712
更新日期:2014-07-01 00:00:00
abstract::Graphene, the mother of all carbon materials, has unlocked a new era of biomedical nanomaterials due to its exceptional biocompatibility, physicochemical and mechanical properties. It is a single atom thick, nanosized, two-dimensional structure and provides high surface area with adjustable surface chemistry to form h...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186X.2018.1437920
更新日期:2018-12-01 00:00:00
abstract::Cytokine inhibiting drugs are much more effective when delivered intracellularly to phagocytic cells in the microencapsulated form. Dexamethasone is a powerful inhibitor of TNF-α cytokine through inhibition of NF-κB which is a gene regulator of multiple pro-inflammatory cytokines. We have determined the effect of micr...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2011.561856
更新日期:2011-11-01 00:00:00
abstract::Polyethylene glycol-modified GoldMag nanoparticles (PGMNs) were synthesized and characterized by several analysis including transmission electron microscopy, dynamic light scattering, Fourier transform infrared spectroscopy, and vibrating sample magnetometer. Here, we showed that the composite nanoparticles have a sat...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611861003801842
更新日期:2011-04-01 00:00:00
abstract::Drug delivery is an important consideration in disease treatment. There are many opportunities for novel methods and technologies to hold promising roles in overcoming traditional obstacles. Delivery systems functionalised to boast synergistic antimicrobial effects, specific targeting, and enhanced bioavailability all...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186X.2017.1419356
更新日期:2018-09-01 00:00:00
abstract::Liposomal encapsulation of doxorubicin (DXR) improves tumor accumulation and reduces adverse effects. One possible strategy for further optimization of this delivery technology would be to design the liposome carrier to release its content within the tumor tissue in response to specific stimuli such as ultrasound (US)...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2010.551401
更新日期:2011-09-01 00:00:00
abstract::We hypothesized that immunoliposomes (ILs) constructed using Fab' from the humanized anti-EGFR monoclonal antibody, EMD72000, can provide efficient intracellular drug delivery in EGFR-overexpressing colorectal tumor cells.ILs were constructed modularly with various MAb fragments, including Fab' from EMD72000 (matuzuma...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860600691049
更新日期:2006-05-01 00:00:00
abstract::Nanoparticles form the fundamental building blocks for many exciting applications in various scientific disciplines due to its unique features such as large surface to mass ratio, targeting potential, ability to adsorbed and carry other compound which makes them suitable for biomedical applications. However, the probl...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186X.2020.1722137
更新日期:2020-06-01 00:00:00
abstract::Necrosis avid agents (NAAs) can be used for diagnose of necrosis-related diseases, evaluation of therapeutic responses and targeted therapeutics of tumor. In order to probe into the effects of molecular skeleton structure on necrosis targeting and clearance properties of radioiodinated dianthrones, four dianthrone com...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/1061186X.2015.1113541
更新日期:2016-01-01 00:00:00
abstract::Tissue plasminogen activator (tPA) is the only FDA approved medical treatment for the ischaemic stroke. However, it associates with some inevitable limitations, including: short therapeutic window, extremely short half-life and low penetration in large clots. Systemic administration may lead to complications such as h...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.1080/1061186X.2017.1365874
更新日期:2018-02-01 00:00:00
abstract::Metal nanoparticles (NPs) may have the potential to overcome problems related to conventional chemotherapy. Metal NPs reported to play a beneficial and powerful role in cancer therapy providing better targeting, gene silencing and drug delivery. Functionalised metal NPs with targeting ligands offer a better control of...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186X.2017.1400553
更新日期:2018-09-01 00:00:00
abstract::Lopinavir (LPV), an efficient drug for HIV infection treatment, was incorporated into biodegradable PLGA nanocapsules (NCs) embedded in microparticles (MCPs) using the spray-drying technique in an attempt to bypass the P-gp efflux and protect the drug from CYP3A pre-systemic metabolism without ritonavir (RTV). SEM obs...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186X.2018.1552275
更新日期:2019-01-01 00:00:00
abstract::There are much said about Pieter Cullis in this special volume honoring him. He was the pioneer to study the role of hexagonal HII phase in membrane fusion and the one who applied this concept for the design of lipid nanoparticles. He was also the first to utilize remote loading techniques for the delivery of amphipat...
journal_title:Journal of drug targeting
pub_type: 传,历史文章,杂志文章
doi:10.3109/1061186X.2016.1172590
更新日期:2016-11-01 00:00:00
abstract::N-(2-hydroxypropyl)methacrylamide (HPMA) copolymers containing doxorubicin and galactosamine have been developed to target the hepatocyte galactose receptor with the aim of organ-specific chemotherapy of primary and metastatic liver disease. Previous biodistribution studies in rats and mice have used tyrosinamide inco...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869308996068
更新日期:1993-01-01 00:00:00
abstract::The high-molecular-weight soluble aggregates of Fab fragments of murine antibodies against cardiac myosin were prepared as a potential long-circulating and low immunogenic pharmaceutical carriers by conjugation of thiolated Fab and Fab modified with succinimidyl 3-(2-pyridyldithio)propionate. The clearance time and bi...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869808997880
更新日期:1998-01-01 00:00:00
abstract::The present study evaluates the prophylactic efficacy of α-tocopherol (α-TOH), resveratrol (RES), and coenzyme Q10 (CoQ10) co-loaded self-nanoemulsifying drug delivery system (α-TOH-RES-CoQ10 SNEDDS) in 7,12-Dimethylbenz[a]anthracene (DMBA) induced breast cancer model. SNEDDS formulation components were rationally sel...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186X.2017.1298603
更新日期:2017-07-01 00:00:00
abstract::Tumour-associated macrophages (TAMs) represent as much as 50% of the solid mass in different types of human solid tumours including lung, breast, ovarian and pancreatic adenocarcinomas. The tumour microenvironment (TME) plays an important role in the polarisation of macrophages into the M1 phenotype, which is tumour-s...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186X.2019.1648478
更新日期:2020-02-01 00:00:00
abstract::Tissue factor (TF) is aberrantly expressed on tumor vascular endothelial cells (VECs) and on cancer cells in many malignant tumors, but not on normal VECs, making it a promising target for cancer therapy. As a transmembrane receptor for coagulation factor VIIa (fVIIa), TF forms a high-affinity complex with its cognate...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/10611860801890093
更新日期:2008-04-01 00:00:00
abstract::Local treatment of middle ear (ME) disease currently requires surgical penetration of the tympanic membrane (TM). We previously discovered 12-mer peptides that are actively transported across the intact TM, a process that could be used for non-invasive drug delivery into the ME. To optimise transport and provide furth...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186X.2017.1347791
更新日期:2018-02-01 00:00:00
abstract::Based on the organotropic characteristics of bile acids towards the liver and the intestine, two novel compounds of the Bamet family, containing at least one bile acid moiety bound to platinum(II), have been synthesized and their cytostatic effect compared to their ability to become accumulated in tumor cells of hepat...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.1080/1061186021000001841
更新日期:2002-08-01 00:00:00
abstract::The objective of this study is to develop and evaluate gastroretentive particulate delivery systems using Riboflavin-5'-monophosphate sodium salt dihydrate (RF5'PNa) as model drug. Poly(acrylic acid)-cysteine and chitosan-4-thiobuthylamidine were evaluated and compared as anionic and cationic polymers for gastroretent...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860903483370
更新日期:2010-06-01 00:00:00
abstract::A targeted delivery system for inflammatory bowel diseases, chitosan-Ca-alginate microparticles efficiently loaded with budesonide (BDS), were designed using one-step spray-drying process. They were eudragit-coated and examined for in vivo efficacy. Experimental colitis was induced by rectal instillation of 2,4,6-trin...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611860903161310
更新日期:2009-12-01 00:00:00
abstract::Liposomes have been prepared from dipalmitoylphosphatidylcholine (DPPC), cholesterol (Chol) and dimethyldioctadecylammonium bromide (DDAB). The cationic vesicles adsorb to biofilms of the skin-associated bacteria Staphylococcus epidermidis, which have a negative charge. Encapsulation of the antibacterial drug vancomyc...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869609015975
更新日期:1996-01-01 00:00:00
abstract::Toxoplasmic encephalitis (TE) is the most common clinical manifestation of reactivated infection with Toxoplasma gondii in immunocompromised patients that is lethal if untreated. The combination of pyrimethamine plus sulfadiazine or clindamycin is the standard therapy for the treatment of TE, but these combinations ar...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611861003733995
更新日期:2011-02-01 00:00:00
abstract::I125 radiolabelled HSA (HSA-I125) was utilised as a probe to quantify protein adsorption onto polystyrene (PS) and Poloxamine 908 coated PS (PS-908) particles. Upon ageing of the HSA-I125 a dramatic increase in the amount of protein adsorbed onto the two particle systems was observed. This phenomenon was not due to la...
journal_title:Journal of drug targeting
pub_type: 杂志文章
doi:10.3109/10611869708995849
更新日期:1997-01-01 00:00:00
abstract::Diabetes is a condition that is not completely treatable but life of a diabetic patient can be smoothed by preventing or delaying the associate conditions like diabetic retinopathy, nephropathy, impaired wound healing process, etc. Apart from conventional methods to regulate diabetic condition, new techniques using si...
journal_title:Journal of drug targeting
pub_type: 杂志文章,评审
doi:10.1080/1061186X.2018.1476518
更新日期:2019-02-01 00:00:00