Abstract:
:Evidence is presented that the chronicity of infection in CBA mice after injection of Brucella abortus 19 is related to a number of factors: (i) the relative resistance of B. abortus to macrophage killing, which allowed some bacteria to survive the peak of macrophage activity occurring at 14 days; (ii) the decline in macrophage activity thereafter (this decline was related in part to the presence of fewer bacteria to stimulate the bactericidal activity and also to specific, active suppressor mechanisms not identified in this study); and (iii) the insensitivity of the persistent Brucella organisms to activated macrophages. This was not due to a selection of genetically resistant bacteria, but possibly to their inaccessibility, either within "incompetent" macrophages or outside macrophages altogether.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Cheers C,Pagram Fdoi
10.1128/IAI.23.2.197-205.1979keywords:
subject
Has Abstractpub_date
1979-02-01 00:00:00pages
197-205issue
2eissn
0019-9567issn
1098-5522journal_volume
23pub_type
杂志文章abstract::Bovine tumor necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) cDNAs were generated by reverse transcription and then by PCR amplification from lipopolysaccharide (LPS)-stimulated alveolar macrophage RNA. The amplified cDNAs were cloned into pPow and expressed in Escherichia coli DH5 alpha. The expr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.2.381-388.1995
更新日期:1995-02-01 00:00:00
abstract::Pseudomonas aeruginosa is considered an extracellular pathogen. Using assays to determine intracellular survival in the presence of gentamicin, we have demonstrated that some strains of P. aeruginosa are able to invade corneal cells during experimental bacterial keratitis in mice. Although intracellular bacteria were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3485-3493.1994
更新日期:1994-08-01 00:00:00
abstract::(DBA/N[female] X CBA/2[male])F1 males have been reported to be deficient in producing antibodies against a number of antigens, including carbohydrates (I. Scher, Adv. Immunol. 35:1-71, 1982). We show that F1 male mice, in contrast to females, made less lipopolysaccharide (LPS)-specific antibodies after immunization wi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1209-1214.1988
更新日期:1988-05-01 00:00:00
abstract::The antigenic cross-reactivity between Treponema pallidum and several pathogenic members of the family Spirochaetaceae was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques. Blots of T. pallidum antigens were incubated with antiserum from rabbits infected or immunize...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.1.116-121.1984
更新日期:1984-10-01 00:00:00
abstract::B-cell-deficient C57B1/6J mice (suppressed from birth with goat anti-mu) and controls (treated from birth with normal goat serum) were infected with Trypanosoma rhodesiense. There was a significant (P less than 0.01) decrease in duration of survival of the mu-suppressed mice compared with that of controls. Whereas bot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.2.434-438.1977
更新日期:1977-11-01 00:00:00
abstract::Exposure of PAM cells, a spontaneously transformed mouse cell line, to brain homogenates from mice infected with scrapie caused a relative decrease in total cell yield, which persisted from passage 2 or 3 to passage 18 after treatment. The effect was elicited by each of the eight independent scrapie isolates tested. L...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.1.163-167.1976
更新日期:1976-07-01 00:00:00
abstract::Neutrophil collagenase (matrix metalloproteinase 8 [MMP-8]) is an important mediator of tissue destruction in inflammatory diseases. Studies of anaerobic periodontal infections have shown that active MMP-8 in gingival crevicular fluid is associated with the degradation of periodontal tissues in progressive periodontit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.5.2319-2326.1999
更新日期:1999-05-01 00:00:00
abstract::Streptococcus pneumoniae sortase A (SrtA) is a transpeptidase that is highly conserved among pneumococcal strains, whose involvement in adhesion/colonization has been reported. We found that intraperitoneal immunization with recombinant SrtA conferred to mice protection against S. pneumoniae intraperitoneal challenge ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01516-08
更新日期:2009-07-01 00:00:00
abstract::Various pathogenic bacteria with the capacity to live within eukaryotic cells activate an apoptotic program in infected host cells. Induction of apoptosis by Listeria monocytogenes in murine dendritic cells and hepatocytes has been described. Here we address the questions of whether and how the pathogen kills macropha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.10.4075-4081.1997
更新日期:1997-10-01 00:00:00
abstract::Several Shigella flexneri mutants with defects in aromatic amino acid and/or purine biosynthesis have been evaluated as vaccines in humans or in animal models. To be suitable as a vaccine, a mutant has to show virulence attenuation, minimal reactogenicity, and a good immunogenic potential in animal models. With this a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.12.7002-7013.2003
更新日期:2003-12-01 00:00:00
abstract::Active immunization with Porphyromonas gingivalis whole-cell preparations has been shown to prevent P. gingivalis infection and oral bone loss. Employing passive antibody transfer and opsonization, we demonstrate with this study that immunization-elicited P. gingivalis-specific immunoglobulin G facilitates clearance o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2408-2411.2004
更新日期:2004-04-01 00:00:00
abstract::Ureaplasma urealyticum is a common commensal of the female lower urogenital tract, yet it has been shown to be an important cause of chorioamnion infection, respiratory and central nervous system disease, and death in premature infants. It has been suggested that only certain serovars are capable of producing invasive...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.891-898.1995
更新日期:1995-03-01 00:00:00
abstract::Studies concerned with the mechanisms responsible for relative resistance or susceptibility of strains of inbred mice to Trypanosoma musculi infections are presented. Treatment with 400 rads of ionizing radiation, silica dust, or trypan blue (reticuloendothelial blocking agents) rendered C3H mice unable to control the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.6.1647-1655.1989
更新日期:1989-06-01 00:00:00
abstract::F1651 and the pyelonephritis-associated pili (Pap) are two members of the type P family of adhesive factors. They play a key role in establishing disease caused by extraintestinal pathogenic Escherichia coli (ExPEC) strains in animals and humans. Both F1651 and Pap are under the control of an epigenetic and reversible...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02510-14
更新日期:2015-05-01 00:00:00
abstract::Cryptosporidial infections in severe combined immune deficient (SCID) mice produce a chronic disease state which in the later stages leads to extraintestinal involvement and hepatic dysfunction. To further characterize the infection dynamics in this model and monitor the changes in the hepatic system, a dose titration...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1691-1695.1994
更新日期:1994-05-01 00:00:00
abstract::The actions of polymyxin B, rabbit polymorphonuclear lysosome extracts, 14 polycationic peptides (including defensin NP-2, cecropin P1, lactoferricin B, and active peptides from cationic protein 18 and bactenecin), EDTA, and Tris on Brucella spp. were studied, with other gram-negative bacteria as controls. Brucella sp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.8.3054-3061.1995
更新日期:1995-08-01 00:00:00
abstract::Mice immunized subcutaneously with heat-killed Escherichia coli were protected from pyelonephritis produced by the intravenous route, but there was little or no protection from ascending infection. No significant protection from ascending or hematogenous pyelonephritis was demonstrated when immunization was accomplish...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.3.330-334.1972
更新日期:1972-09-01 00:00:00
abstract::Mice were vaccinated with 300 micrograms of BCG cell walls (BCG-CW) in oil-in-water emulsion intravenously or with a high or low dose of living BCG by inhalation (BCG-HD or BCG-LD, respectively). The consequences of vaccination were evaluated in terms of the growth of BCG in the lungs and spleen, lung and spleen weigh...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::Q fever is a zoonotic disease of worldwide significance caused by the obligate intracellular bacterium Coxiella burnetii. Humans with Q fever may experience an acute flu-like illness and pneumonia and/or chronic hepatitis or endocarditis. Various markers demonstrate significant phylogenetic separation between and clus...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00851-09
更新日期:2009-12-01 00:00:00
abstract::A Mycobacterium tuberculosis strain disrupted in the AraC homologue Rv1931c was isolated. The mutant strain exhibited reduced survival both in macrophages and in a mouse infection model, with survival being restored on complementation with the Rv1931c gene. These results suggest that Rv1931c regulates genes important ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5483-5486.2004
更新日期:2004-09-01 00:00:00
abstract::The Haemophilus ducreyi 35000HP genome encodes a homolog of the CpxRA two-component cell envelope stress response system originally characterized in Escherichia coli. CpxR, the cytoplasmic response regulator, was shown previously to be involved in repression of the expression of the lspB-lspA2 operon (M. Labandeira-Re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00678-10
更新日期:2010-11-01 00:00:00
abstract::FcRgamma and interleukin-10 (IL-10) are both required for chronic disease in C57BL/6 mice with Leishmania mexicana parasite infection. FcRgamma is a component of several different FcRs and may be a component of some T-cell receptors. The initial antibody response to L. mexicana is an immunoglobulin G1 (IgG1) response,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00316-07
更新日期:2008-02-01 00:00:00
abstract::The rate of clearance of Vibrio fetus var. venerealis was measured by using in vivo and in vitro methods. The rate of clearance from the circulating blood increased in immune rabbits and reached a plateau 4 days after immunization. Thereafter in vivo clearance did not increase further despite rising agglutinin titers....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.6.815-818.1971
更新日期:1971-06-01 00:00:00
abstract::Streptococcus sanguis 804 (NCTC 10904) was grown ih batch culture at constant pH. and the glucosyltransferase activity of the supernatant was assayed over a 40-h growth period. The optimum pH for enzyme production was 7.0 to 7.2. During growth of the culture, three reproducible phases of enzyme activity were observed....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.50-58.1977
更新日期:1977-01-01 00:00:00
abstract::We investigated the role of bacterial adherence and hemolysin production from Escherichia coli parent and genetically cloned strains as to their effects on histamine release from rat mast cells and leukotriene generation from human polymorphonuclear granulocytes. These mediators were involved in the induction of infla...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.1.271-278.1985
更新日期:1985-10-01 00:00:00
abstract::UV-inactivated Sendai virus is mitogenic for murine splenocytes, whereas infectious Sendai virus kills spleen cells in vitro. The isolated hemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins of Sendai virus are also mitogenic for cultured mouse spleen cells. A mixture of these glycoproteins (1 microgram/well...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.592-600.1983
更新日期:1983-05-01 00:00:00
abstract::Cross-reactive humoral immune responses between antigens of different developmental stages of the worm Schistosoma mansoni have previously been demonstrated. In contrast, information on antigenic cross-reactivity at the T-cell level is still very sparse. The present study examined the cross-reactive T-cell responses t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.9.3237-3242.1991
更新日期:1991-09-01 00:00:00
abstract::The relationship between the sequence of Neisseria gonorrhoeae pilin and its quaternary assembly into pilus fibers was studied with a set of site-directed antibody probes and by mapping the specificities of antipilus antisera with peptides. Buried and exposed peptides in assembled pili were identified by competitive i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.2.644-652.1996
更新日期:1996-02-01 00:00:00
abstract::Ehrlichia chaffeensis, a tick-transmitted obligate intracellular rickettsial agent, causes human monocytic ehrlichiosis. In recent reports, we described substantial advances in developing random and targeted gene disruption methods to investigate the functions of E. chaffeensis genes. We reported earlier that the Hima...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00316-20
更新日期:2020-09-18 00:00:00
abstract::The importance of CD4+ cells and gamma interferon (IFN-gamma) in the resolution of established Cryptosporidium parvum infection was investigated with a murine model of cryptosporidiosis in severe combined immunodeficient (SCID) mice. C. parvum-infected SCID mice were reconstituted with spleen cells from immunocompeten...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3928-3932.1993
更新日期:1993-09-01 00:00:00