Abstract:
:Streptococcus pneumoniae sortase A (SrtA) is a transpeptidase that is highly conserved among pneumococcal strains, whose involvement in adhesion/colonization has been reported. We found that intraperitoneal immunization with recombinant SrtA conferred to mice protection against S. pneumoniae intraperitoneal challenge and that the passive transfer of immune serum before intraperitoneal challenge was also protective. Moreover, by using the intranasal challenge model, we observed a significant reduction of bacteremia when mice were intraperitoneally immunized with SrtA, while a moderate decrease of lung infection was achieved by intranasal immunization, even though no influence on nasopharynx colonization was seen. Taken together, our results suggest that SrtA is a good candidate for inclusion in a multicomponent, protein-based, pneumococcal vaccine.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Gianfaldoni C,Maccari S,Pancotto L,Rossi G,Hilleringmann M,Pansegrau W,Sinisi A,Moschioni M,Masignani V,Rappuoli R,Del Giudice G,Ruggiero Pdoi
10.1128/IAI.01516-08subject
Has Abstractpub_date
2009-07-01 00:00:00pages
2957-61issue
7eissn
0019-9567issn
1098-5522pii
IAI.01516-08journal_volume
77pub_type
杂志文章abstract::The reproducibility of an experimental result is a fundamental assumption in science. Yet, results that are merely confirmatory of previous findings are given low priority and can be difficult to publish. Furthermore, the complex and chaotic nature of biological systems imposes limitations on the replicability of scie...
journal_title:Infection and immunity
pub_type: 社论
doi:10.1128/IAI.00908-10
更新日期:2010-12-01 00:00:00
abstract::Subtilase cytotoxin (SubAB) was first isolated from a Shiga toxigenic Escherichia coli (STEC) strain that was responsible for an outbreak of hemolytic-uremic syndrome and is the prototype of a new family of AB(5) cytotoxins. SubAB is a subtilase-like serine protease, and upon uptake by host cells, it is trafficked to ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00801-10
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.12.3988-3995.1990
更新日期:1990-12-01 00:00:00
abstract::In the present investigation we studied the nature of a Shigella flexneri O antigen, the type III antigen. No structural entity has yet been attributed to this antigen. Phenol-water-extracted lipopolysaccharides (LPSs) from all S. flexneri serotypes possessing either group 6 antigen or both type III antigen and group ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.1.110-115.1986
更新日期:1986-07-01 00:00:00
abstract::A set of four monoclonal antibodies was produced against a highly purified pepsin extract of type 5 streptococcal M protein. Three of the four antibodies cross-reacted with purified M proteins from heterologous serotypes and opsonized the respective heterologous organisms. Our studies suggest that monoclonal antibodie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.1.267-269.1984
更新日期:1984-10-01 00:00:00
abstract::Comparison of the susceptibility of outbred OF1 and inbred BALB/c, C57BL/6, DBA/2, and CBA mice to heat-stable toxin (STa) of enterotoxigenic Escherichia coli was made at different levels of induced secretion. STa was able to elicit fluid accumulation into the intestine of each strain of mice; however, quantitatively ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.8.3117-3121.1992
更新日期:1992-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.1.130-135.1981
更新日期:1981-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00449-08
更新日期:2009-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.1848-1854.1998
更新日期:1998-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.782-786.1985
更新日期:1985-06-01 00:00:00
abstract::Guinea pig and mouse peritoneal macrophages in culture transport glucose by a specific, saturable system with characteristics compatible with facilitated diffusion. Phagocytosis of killed staphylococci or polystyrene latex spheres results in a significant increase in uptake of 2-deoxy-d-glucose. Reciprocal plot analys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1391-1396.1974
更新日期:1974-12-01 00:00:00
abstract::Rabbit-specific enteropathogenic Escherichia coli (REPEC) is an attaching and effacing pathogen of young rabbits. Using signature-tagged mutagenesis, we identified several known colonization factors of REPEC as well as a gene predicted to encode a novel autotransporter protein. This novel gene was termed rpeA for REPE...
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pub_type: 杂志文章
doi:10.1128/IAI.00972-06
更新日期:2007-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.1.72-79.2000
更新日期:2000-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.895-899.1982
更新日期:1982-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2728-2735.1998
更新日期:1998-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.9.5036-5044.2002
更新日期:2002-09-01 00:00:00
abstract::The generation of nitric oxide (NO) by activated macrophages is believed to control mycobacterial infection in the murine system. In this study we examined the effect of Mycobacterium bovis BCG infection on the L-arginine-dependent NO pathway in J774.1 murine macrophages. We have confirmed previous results by demonstr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.9.5823-5831.2001
更新日期:2001-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.4.1351-1356.1996
更新日期:1996-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.11.2856-2860.1988
更新日期:1988-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1996-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.4.887-892.1990
更新日期:1990-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1179-1184.2003
更新日期:2003-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2004-11-01 00:00:00
abstract::Naturally occurring antibodies against [Gal alpha-1,3-Gal] structures (anti-Gal antibodies) are the primary effectors of human hyperacute rejection (HAR) of nonhuman tissue. Unlike most mammals, humans lack a functional alpha-1,3-galactosyltransferase (GalT) gene and produce abundant anti-Gal antibodies, putatively in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.11.6215-6222.2002
更新日期:2002-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06339-11
更新日期:2012-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.50-61.2005
更新日期:2005-01-01 00:00:00
abstract::The complement system plays a key role in resistance to cryptococcosis. In the present study, we examined several factors that influence the binding of C3 cleavage fragments to Cryptococcus neoformans. Binding of C3 was determined by using normal human serum supplemented with 125I-labeled C3. Incubation of encapsulate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.1.1-5.1986
更新日期:1986-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.3.707-713.1980
更新日期:1980-03-01 00:00:00
abstract::A nonhuman primate model for malaria vaccine development allowing reliable, stringent sporozoite challenge and evaluation of both cellular and antibody responses is needed. We therefore constructed a multicomponent, multistage DNA vaccine for the simian malaria species Plasmodium knowlesi including two preerythrocytic...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.9.5565-5572.2001
更新日期:2001-09-01 00:00:00
abstract::The bactericidal and opsonizing effects of normal human serum on six strains of Salmonella typhimurium LT-2 having different lipopolysaccharide (LPS) composition were demonstrated through five indices. Complement activity in the presence of antibody was important for the opsonization of all six strains and for the bac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.3.647-653.1985
更新日期:1985-09-01 00:00:00