Abstract：
:The Semliki Forest virus capsid protein contains a chymotrypsin-like protease domain that must fold before it can autocatalytically cleave the protein from a larger polyprotein precursor. Here we analyse this cleavage in living mammalian and prokaryotic cells, and find that it occurs immediately after the emergence of the protease domain from the ribosome during protein synthesis. The acquisition of the native conformation of this domain thus occurs rapidly and at the same time as translation. It does not require termination of translation or release from the ribosome, and nor does it involve Hsp70 binding. These results provide direct evidence that protein folding can occur co-translationally in the cytosol of both prokaryotes and eukaryotes.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Nicola AV,Chen W,Helenius Adoi
10.1038/14032keywords:
subject
Has Abstractpub_date
1999-10-01 00:00:00pages
341-5issue
6eissn
1465-7392issn
1476-4679journal_volume
1pub_type
杂志文章abstract：:Previous analyses of labelled clones of cells within the developing nervous system of the mouse have indicated that descendants are initially dispersed rostrocaudally followed by more local proliferation, which is consistent with the progressing node's contributing descendants from a resident population of progenitor ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35078535
更新日期：2001-06-01 00:00:00
abstract：:The formation of axon trajectories requires integration of local adhesive interactions with directional information from attractive and repulsive cues. Here, we show that these two types of information are functionally integrated; activation of the transmembrane receptor Roundabout (Robo) by its ligand, the secreted r...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb858
更新日期：2002-10-01 00:00:00
abstract：:Cytokinesis requires the spatio-temporal coordination of cell-cycle control and cytoskeletal reorganization. Members of the Rho-family of GTPases are crucial regulators of this process and assembly of the contractile ring depends on local activation of Rho signalling. Here, we show that the armadillo protein p0071, un...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1504
更新日期：2006-12-01 00:00:00
abstract：:BACE1 activity is significantly increased in the brains of Alzheimer's disease patients, potentially contributing to neurodegeneration. The voltage-gated sodium channel (Na(v)1) beta2-subunit (beta2), a type I membrane protein that covalently binds to Na(v)1 alpha-subunits, is a substrate for BACE1 and gamma-secretase...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1602
更新日期：2007-07-01 00:00:00
abstract：:Breast cancer brain metastasis (BCBM) is a devastating disease. Radiation therapy remains the mainstay for treatment of this disease. Unfortunately, its efficacy is limited by the dose that can be safely applied. One promising approach to overcoming this limitation is to sensitize BCBMs to radiation by inhibiting thei...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-020-00586-6
更新日期：2020-10-01 00:00:00
abstract：:Protein ubiquitination regulates many cellular processes, including protein degradation, signal transduction, DNA repair and cell division. In the classical model, a uniform polyubiquitin chain that is linked through Lys 48 is required for recognition and degradation by the 26S proteasome. Here, we used a reconstitute...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1436
更新日期：2006-07-01 00:00:00
abstract：:N-WASP is a major cytoskeletal regulator that stimulates Arp2/3-mediated actin nucleation. Here, we identify a nucleation-independent pathway by which N-WASP regulates the cytoskeleton and junctional integrity at the epithelial zonula adherens. N-WASP is a junctional protein whose depletion decreased junctional F-acti...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2290
更新日期：2011-07-24 00:00:00
abstract：:The actin filament (F-actin) cytoskeleton associates dynamically with the plasma membrane and is thus ideally positioned to participate in endocytosis. Indeed, a wealth of genetic and biochemical evidence has confirmed that actin interacts with components of the endocytic machinery, although its precise function in en...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1025
更新日期：2003-08-01 00:00:00
abstract：:Epigenetic mechanisms participate in the regulation of gene transcription in eukaryotes. Two studies in yeast have revealed an additional mechanism for controlling global gene transcription that is based on an inherited self-perpetuating change in the conformation of two different components of key transcriptional reg...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb0309-241
更新日期：2009-03-01 00:00:00
abstract：: ...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/s41556-018-0246-5
更新日期：2018-12-01 00:00:00
abstract：:Primary cilia are required for vertebrate cells to respond to specific intercellular signals. Here we define when and where primary cilia appear in the mouse embryo using a transgenic line that expresses ARL13B-mCherry in cilia and Centrin 2-GFP in centrosomes. Primary cilia first appear on cells of the epiblast at E6...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3091
更新日期：2015-02-01 00:00:00
abstract：:Tumour growth and invasiveness require extracellular matrix (ECM) degradation and are stimulated by the GALA pathway, which induces protein O-glycosylation in the endoplasmic reticulum (ER). ECM degradation requires metalloproteases, but whether other enzymes are required is unclear. Here, we show that GALA induces th...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-020-00590-w
更新日期：2020-11-01 00:00:00
abstract：:In the version of this Article originally published, ref. 52 was incorrectly only attributed to its corresponding author, Fre, S., and an older title was used. The correct citation should have been: Lilja, A. M. et al. Clonal analysis of Notch1-expressing cells reveals the existence of unipotent stem cells that retain...
journal_title：Nature cell biology
pub_type： 已发布勘误
doi：10.1038/s41556-018-0164-6
更新日期：2018-09-01 00:00:00
abstract：:In the unicellular eukaryote Saccharomyces cerevisiae, Cln3-cyclin-dependent kinase activity enables Start, the irreversible commitment to the cell division cycle. However, the concentration of Cln3 has been paradoxically considered to remain constant during G1, due to the presumed scaling of its production rate with ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-019-0413-3
更新日期：2019-11-01 00:00:00
abstract：:It is now well established that stromal interaction molecule 1 (STIM1) is the calcium sensor of endoplasmic reticulum stores required to activate store-operated calcium entry (SOC) channels at the surface of non-excitable cells. However, little is known about STIM1 in excitable cells, such as striated muscle, where th...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1731
更新日期：2008-06-01 00:00:00
abstract：:Current models for the export of messenger RNA share the notion that the highly abundant class of nuclear RNA-binding proteins--the hnRNP proteins--have a key role in exporting RNA. But recent studies have led to a new understanding of several non-hnRNP proteins, including SR proteins and the conserved mRNA export fac...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb0901-e201
更新日期：2001-09-01 00:00:00
abstract：:ES-cell-based cardiovascular repair requires an in-depth understanding of the molecular mechanisms underlying the differentiation of cardiovascular ES cells. A candidate cardiovascular-fate inducer is the bHLH transcription factor MesP1. As one of the earliest markers, it is expressed specifically in almost all cardio...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1696
更新日期：2008-03-01 00:00:00
abstract：:Phagocytic cells engulf their prey into vesicular structures called phagosomes, of which a certain proportion becomes demarcated for enhanced maturation by a process called LC3-associated phagocytosis (LAP). Light has now been shed on the molecular requirements of LAP, establishing a central role for the protein Rubic...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb3197
更新日期：2015-07-01 00:00:00
abstract：:During meiosis, homologous chromosomes are sorted into pairs and are then intimately aligned, or synapsed, along their lengths while a proteinaceous structure, the synaptonemal complex, is assembled between them. However, little is known about how chromosomes first recognise each other. Here we show, by comparing the ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1168
更新日期：2004-09-01 00:00:00
abstract：:Brown adipose tissue is a key metabolic organ that oxidizes fatty acids and glucose to generate heat. Through epigenomic analyses of multiple adipose depots, the transcription factor nuclear factor I-A (NFIA) is now shown to drive the brown fat genetic program through binding to lineage-specific cis-regulatory element...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3591
更新日期：2017-08-31 00:00:00
abstract：:RNA interference has become an indispensable tool for loss-of-function studies across eukaryotes. By enabling stable and reversible gene silencing, shRNAs provide a means to study long-term phenotypes, perform pool-based forward genetic screens and examine the consequences of temporary target inhibition in vivo. Howev...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/ncb2895
更新日期：2014-01-01 00:00:00
abstract：:Different sites of plasma membrane attachment may underlie functional differences between isoforms of Ras. Here we show that palmitoylation and farnesylation targets H-ras to lipid rafts and caveolae, but that the interaction of H-ras with these membrane subdomains is dynamic. GTP-loading redistributes H-ras from raft...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35070050
更新日期：2001-04-01 00:00:00
abstract：:Lamina-associated polypeptide (LAP) 2alpha is a chromatin-associated protein that binds A-type lamins. Mutations in both LAP2alpha and A-type lamins are linked to human diseases called laminopathies, but the molecular mechanisms are poorly understood. The A-type lamin-LAP2alpha complex interacts with and regulates ret...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1793
更新日期：2008-11-01 00:00:00
abstract：:Many pro-apoptotic signals activate caspase-9, an initiator protease that activates caspase-3 and downstream caspases to initiate cellular destruction. However, survival signals can impinge on this pathway and suppress apoptosis. Activation of the Ras-Raf-MEK-ERK mitogen-activated protein kinase (MAPK) pathway is asso...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1005
更新日期：2003-07-01 00:00:00
abstract：:Dozens of proteins capture, polymerize and reshape the clathrin lattice during clathrin-mediated endocytosis (CME). How or if this ensemble of proteins is organized in relation to the clathrin coat is unknown. Here, we map key molecules involved in CME at the nanoscale using correlative super-resolution light and tran...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3498
更新日期：2017-04-01 00:00:00
abstract：:Work over the past two years has led to a breakthrough in our understanding of the molecular basis of the directionality of the kinesin motor proteins. This breakthrough has come first from the reversal of directionality of the kinesin-related motor Ncd, followed closely by the reversal of kinesin's directionality and...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
doi：10.1038/14113
更新日期：1999-10-01 00:00:00
abstract：:The mitogen-activated protein kinase (MAPK) network is a conserved signalling module that regulates cell fate by transducing a myriad of growth-factor signals. The ability of this network to coordinate and process a variety of inputs from different growth-factor receptors into specific biological responses is, however...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1543
更新日期：2007-03-01 00:00:00
abstract：:Accurate chromosome segregation during cell division in metazoans relies on proper chromosome congression at the equator. Chromosome congression is achieved after bi-orientation to both spindle poles shortly after nuclear envelope breakdown, or by the coordinated action of motor proteins that slide misaligned chromoso...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3060
更新日期：2014-12-01 00:00:00
abstract：:Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) involves a marked reorganization of chromatin. To identify post-translational histone modifications that change in global abundance during this process, we have applied a quantitative mass-spectrometry-based approach. We found that iPSCs, compa...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2768
更新日期：2013-07-01 00:00:00
abstract：:Growing microtubule end regions recruit a variety of proteins collectively termed +TIPs, which confer local functions to the microtubule cytoskeleton. +TIPs form dynamic interaction networks whose behaviour depends on a number of potentially competitive and hierarchical interaction modes. The rules that determine whic...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2999
更新日期：2014-08-01 00:00:00
