Abstract：
:The expression of voltage-gated potassium channels plays an important role in the acquisition of membrane excitability in neurons. We examined the expression pattern of genes in developing cerebellar granule neurons in vivo and in vitro. In situ hybridization of Kv3.1 mRNA demonstrated that the gene was expressed at high levels in the external granule layer (EGL) as well as in the internal granule layer (IGL) at all postnatal stages (P) examined (from P3 to P10). In contrast, Kv4.2 mRNA was detected in the premigratory zone (PMZ) of the EGL, but not in the proliferative zone (PLZ), in addition to the IGL. This indicates that Kv4.2 gene expression initiates in the postmitotic migrating neurons. We also examined the expression of the channel genes in microexplant culture systems. Kv3.1 polypeptide was detected in parallel fibers of granule cells at 2 days in vitro, and the expression continued in later stages. The signal of Kv4.2 protein was very low at 2 days in vitro; however, the number of positive cells and the intensity of the signals were increased at 6 days in vitro. These in vitro observations matched those in vivo and our previous electrophysiological studies in which we demonstrated that delayed- rectifier-type current was predominant in the immature granule cells followed by the later appearance of A-type current. The patterns of K(+) channel expression suggest that sequential expression of these channel genes primarily determines the membrane excitability.
journal_name
Dev Neuroscijournal_title
Developmental neuroscienceauthors
Shibata R,Wakazono Y,Nakahira K,Trimmer JS,Ikenaka Kdoi
10.1159/000017370keywords:
subject
Has Abstractpub_date
1999-01-01 00:00:00pages
87-93issue
2eissn
0378-5866issn
1421-9859pii
17370journal_volume
21pub_type
杂志文章abstract：:Disorders primarily affecting the nervous system comprise approximately one third of all established Mendelian genetic diseases in man. Recombinant DNA technology provides new approaches to the diagnosis and elucidation of the molecular pathology of these disorders. For a small but increasing number of disorders the D...
journal_title：Developmental neuroscience
pub_type： 杂志文章,评审
doi：10.1159/000112346
更新日期：1983-01-01 00:00:00
abstract：:Traumatic brain injury (TBI) is the leading cause of death and disability in children. Evidence-based guidelines for the management of this population are available; however, the data highlight significant deficiencies with few treatment standards or guidelines. Considering the limited availability of resources, it is...
journal_title：Developmental neuroscience
pub_type： 杂志文章,评审
doi：10.1159/000094153
更新日期：2006-01-01 00:00:00
abstract：:Neuronal heterotopia is a malformation of cortical development that is closely associated with epilepsy in humans. Despite emerging interest in the structure and function of the heterotopic cortex, little is known about the membrane properties and synaptic connections of these displaced neurons. We used whole-cell pat...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000017386
更新日期：1999-11-01 00:00:00
abstract：:Chick embryo retina neurons in culture extend neurites and form synapses. Seven different culture substrates were compared to determine which culture surface would produce the best neuronal survival and neurite extension. The order of survival of retina neurons during 7 days of culture is salt-precipitated collagen gr...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000112705
更新日期：1982-01-01 00:00:00
abstract：:We report on the effects of etiology and age on the linguistic outcomes in a large pediatric hemispherectomy population. Four populations were considered separately: cortical dysplasia (multilobar involvement), Rasmussen's encephalitis, infarction as a primary etiology and, fourth, children who failed to develop langu...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000017396
更新日期：1999-11-01 00:00:00
abstract：:Evidence from retroviral marking techniques and immortalized cell lines indicates that multipotential stem cells exist in many areas of the developing central nervous system. However, the factors that influence the commitment of these stem cells into distinct neuronal or glial lineages are not known. We have created a...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000111301
更新日期：1995-01-01 00:00:00
abstract：:The medial frontal cortex (MFC) is involved in the temporal organization of behaviour. It receives timing information from the master circadian clock in the suprachiasmatic nucleus (SCN), and exhibits daily oscillations in gene expression itself. In this study, we evaluate various properties of circadian rhythms of lo...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000232559
更新日期：2009-01-01 00:00:00
abstract：:The fine structure of pituicytes was investigated between 15 days of fetal life and 120 days postnatum. Prior to the penetration of neurosecretory axons into the neural lobe a uniform population of undifferentiated pituicytes is presented. Coincidental with the first appearance of neurosecretory axons in the neural lo...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000112744
更新日期：1981-01-01 00:00:00
abstract：:Proper eye formation depends on specific interactions between neural and ectodermal tissues coupled with temporally distinct gene expression and a regulated sequence of signaling events. The homeobox gene Pax6 is vitally important to the entire process of eye development in both vertebrates and invertebrates. Pax6 exp...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000082273
更新日期：2004-01-01 00:00:00
abstract：:L-Ornithine decarboxylase (ODC), the rate-limiting enzyme of polyamine biosynthesis and thus a unique marker of the proliferation and maturation processes, has been localized by means of immunohistochemistry in the human central nervous system. The enzyme protein has not only been found in the developing nervous syste...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000111320
更新日期：1993-01-01 00:00:00
abstract：:There is an important unmet need to further improve the outcome of neonatal encephalopathy in term infants. Meta-analyses of large controlled trials now suggest that maternal magnesium sulfate (MgSO4) therapy is associated with a reduced risk of cerebral palsy and gross motor dysfunction after premature birth, but tha...
journal_title：Developmental neuroscience
pub_type： 杂志文章,评审
doi：10.1159/000362206
更新日期：2014-01-01 00:00:00
abstract：:Traumatic brain injury (TBI) is predominantly a clinical problem of young persons, resulting in chronic cognitive and behavioral deficits. Specifically, the physiological response to a diffuse biomechanical injury in a maturing brain can clearly alter normal neuroplasticity. To properly evaluate and investigate develo...
journal_title：Developmental neuroscience
pub_type： 杂志文章,评审
doi：10.1159/000094163
更新日期：2006-01-01 00:00:00
abstract：:Maternal peptides from the hypothalamic-pituitary-adrenal (HPA) axis rise during human pregnancy. The effects of circulating maternal adrenocorticotropin (ACTH) and beta-endorphin (BE) on human fetal behavior was determined in 135 women during their 32nd week of gestation. Fetal behavior was measured by assessing hear...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000071467
更新日期：2003-01-01 00:00:00
abstract：:We have previously reported that a developmental axon-sparing striatal injury induced by hypoxia-ischemia results in a diminished adult number of substantia nigra (SN) tyrosine hydroxylase (TH)-positive neurons, in the absence of direct nigral injury. To examine the specific role of striatal injury, we made selective ...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000111684
更新日期：1992-01-01 00:00:00
abstract：:A total of 216 male and female albino Wistar rats (Rattus norvegicus) between 10 and 60 days old from 42 litters were studied to determine mast cell characteristics within the thalamus. Whereas the mean numbers of mast cells within both the parenchyma and leptomeninges (combined) did not change appreciably after 15 da...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000112759
更新日期：1981-01-01 00:00:00
abstract：:Rats were exposed to mild hyperoxia and hypoxia by the administration of oxygen/air and nitrogen/air mixtures through plastic tubing held close to their snouts for periods of 3 min. Changes in tissue oxygen were monitored at an implanted carbon paste electrode; local cerebral blood flow (rCBF) at an implanted platinum...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000017298
更新日期：1998-01-01 00:00:00
abstract：:Glutamine synthesis, the major pathway of ammonia detoxification, and the intracellular concentration of organic osmolytes in primary astrocytes and F98 glioma cells were investigated with multinuclear magnetic resonance spectroscopy. Acute exposure to ammonia (3 h incubation with NH4Cl) raised the concentration of gl...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000017339
更新日期：1998-01-01 00:00:00
abstract：:Plexin is a type I membrane protein which was identified in Xenopus nervous system by hybridoma technique. Molecular cloning studies demonstrated that the extracellular segment of the plexin protein possesses three internal repeats of cysteine cluster which are homologous to the cysteine-rich domain of the c-met proto...
journal_title：Developmental neuroscience
pub_type： 杂志文章,评审
doi：10.1159/000111192
更新日期：1997-01-01 00:00:00
abstract：:Perinatal hypoxic ischemic (HI) brain injury is a leading cause of long-term neurological handicap in newborn babies. Recently, excessive activity of matrix metalloproteinases (MMPs), and in particular MMP-9, has been implicated in the aetiology of HI injuries to the immature brain. Our previous study suggested that M...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000343257
更新日期：2012-01-01 00:00:00
abstract：:The myelin-associated glycoprotein (MAG) is a 100 K-dalton, integral membrane glycoprotein containing 30% carbohydrate that is in central and peripheral myelin sheaths. Recent research on its function during normal myelinogenesis and possible roles in the pathology of demyelinating diseases is reviewed. Emphasis is pl...
journal_title：Developmental neuroscience
pub_type： 杂志文章,评审
doi：10.1159/000112356
更新日期：1983-01-01 00:00:00
abstract：:Extracts prepared from embryonic, neonatal and adult rat brain were examined for the presence of oligodendroglial mitogens. Brain-derived mitogenic activities were found in all developmental stages with specific activity increasing during neonatal development. Extracts from postnatal day 7 contained the highest specif...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000112301
更新日期：1985-01-01 00:00:00
abstract：:Transient early cerebral laminar organization resulting from normal developmental events has been revealed in human beings through histology and imaging studies. DTI studies have postulated that the fractional anisotropy (FA)-based differentiation of different laminar structures reflects both differing cellular densit...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000229500
更新日期：2009-01-01 00:00:00
abstract：:Hypoxic-ischemic brain injury is a leading cause of neurodevelopmental morbidities in preterm and full-term infants. Blood-brain barrier dysfunction represents an important component of perinatal hypoxic-ischemic brain injury. The extracellular matrix (ECM) is a vital component of the blood-brain barrier. Matrix metal...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000489700
更新日期：2018-01-01 00:00:00
abstract：:In the presence of nerve growth factor (NGF) PC12 pheochromocytoma cells develop properties of sympathetic neurons and extend long microtubule-containing neurites. PC12 cell microtubule protein was isolated using an assembly and disassembly procedure, either directly from an unlabeled cell extract or by copolymerizati...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000112330
更新日期：1983-01-01 00:00:00
abstract：:A study was made on the development of cortical synapses in the gracile nucleus of rats using degeneration methods. A total of 46 animals, 1 adult and 45 neonates whose ages varied from 1 to 7 days, had the right somatosensory motor cortex destroyed. The survival period varied from 1 to 30 days. Identification of axon...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000112118
更新日期：1994-01-01 00:00:00
abstract：:All known actions of cAMP in the brain require cAMP-dependent protein kinase (cAMPdPK), which consists of regulatory (R) and catalytic (C) subunits (R2C2). Using homologous rat cDNAs for all known cAMPdPK subunit isoforms found in the brain (RI alpha, RI beta, RII alpha, RII beta, C alpha, C beta) we observe that, in ...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000112140
更新日期：1991-01-01 00:00:00
abstract：:In vertebrate species, the neuropeptide oxytocin (OT) has been implicated in neural and behavioral development. Although several OT-like peptides have been characterized in invertebrate species, the ontogenesis of the OT-like system has not yet been described in these species. Thus, the aim of the present study was to...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000235942
更新日期：2010-03-01 00:00:00
abstract：:Prenatal stress has been associated with detrimental outcomes of pregnancy, including altered brain development leading to behavioural pathologies. The neurosteroid allopregnanolone has been implicated in mediating some of these adverse outcomes following prenatal stress due to its potent inhibitory and anxiolytic eff...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000437302
更新日期：2015-01-01 00:00:00
abstract：:The rate of glutaminase-dependent metabolism of glutamine in intact astrocytes was determined under conditions in which the extracellular concentration of glutamine was varied between 0.2 and 3.2 mM glutamine for control and dibutyryl cyclic AMP (dBcAMP)-treated cells. Glutamine metabolism by intact cells increased wi...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000111410
更新日期：1996-01-01 00:00:00
abstract：:In adult rats, kainic acid-induced status epilepticus markedly reduces GluR2 (the alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid, AMPA subunit that limits Ca2+ permeability), receptor mRNA in the vulnerable CA3 and may contribute to delayed neurodegeneration. In rat pups resistant to kainate seizure-induced...
journal_title：Developmental neuroscience
pub_type： 杂志文章
doi：10.1159/000111257
更新日期：1997-01-01 00:00:00
