Abstract:
:The control of progenitor cell proliferation in concert with terminal differentiation during embryonic development is poorly understood. The present paper examines this issue in the different cell lineages of the fetal mouse pituitary. Mouse fetuses were pulse-exposed to 3H-thymidine (3H-T) on a single day between embryonic day (E) 10 and E16 (prior to the onset of hormone phenotype expression) and the 3H-T labeling index of each cell type determined 3 or 4 days later (E13-19), when hormone phenotypes were detectable. In the pars tuberalis primordium, TSHbeta appeared from E13. Of these cells 75.5% were labeled when 3H-T had been administered on E10. Label decreased to 40.8% when it had been incorporated on E11 and was negligible (4.2%) when it had been taken up on E12. In the pars distalis, ACTH appeared on E13, TSHbeta, and PRL on E14, LHbeta/FSHbeta on E15 and GH on E16. When examined on E16, all these cell types were labeled for 50-60% if 3H-T had been injected on E12, but this number dropped to about 15% when 3H-T had been given on E13. Only 5-10% of the hormonal cells had taken up label when E14, 15, and 16 were the days of 3H-T administration. The decline in overall labeling index (LI) within both parts of the pituitary was significantly smaller than that in the hormone expressing cells. It is concluded that an outspoken decline in proliferation of the cells destined to become hormone-expressing cell types occurs one to several days before these hormones come to expression. In the pars distalis, this decline occurs at a common time point i.e. between E12 and E13 for each cell type. Pars tuberalis and pars distalis TSHbeta cells show distinct 3H-T labeling profiles, suggesting distinct cell lineage sources for each.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Seuntjens E,Denef Cdoi
10.1016/s0303-7207(99)00028-3keywords:
subject
Has Abstractpub_date
1999-04-25 00:00:00pages
57-63issue
1-2eissn
0303-7207issn
1872-8057pii
S0303-7207(99)00028-3journal_volume
150pub_type
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