Abstract:
:Background: Epithelial-mesenchymal transition (EMT) plays an important role in the invasion and migration during cancer metastasis. Wnt3a is one of the ligands in canonical Wnt/β-catenin signaling pathway, which contributes to the carcinogenesis and progression of lung cancer cell lines. The aim of this study was to evaluate the association between Wnt3a and EMT-related proteins (E-cadherin and N-cadherin), and to further investigate its impact on prognosis of lung adenocarcinoma patients. Methods: A total of 147 lung adenocarcinoma patients were included and their clinicopathological characteristics were collected in this retrospective study. The expression levels of Wnt3a, E-cadherin and N-cadherin in post-surgery cancerous and adjacent normal tissues were assessed by immunohistochemistry. The association between Wnt3a and EMT-related proteins and their prognostic values were systematically evaluated. HCC827 and PC9 cell lines were treated with Wnt3a to detect the expression of EMT-related and Wnt/β-catenin signaling-associated proteins, as well as the in vitro migration and invasion abilities. Results: High Wnt3a expression level was significantly associated with low E-cadherin (P<0.001) and high N-cadherin (P<0.001) expression levels in lung adenocarcinoma tissues. Besides, high Wnt3a level predicted poorer lung adenocarcinoma survival by univariate Cox analysis (P=0.001), while the multivariate result was not significant (P=0.355). Subgroup analysis suggested that the prognostic value of Wnt3a expression level was significant in stage T1-T2 (log rank P=0.003) and stage N0 (log rank P=0.031) patients. The multivariate Cox analysis suggested N-cadherin was an independent prognostic factor for lung adenocarcinoma patients (P=0.012). After including these markers into a nomogram, the Harrell's C-index of the nomogram was 0.755. The decision-curve analysis of our nomogram performed net benefit at the threshold probability from 21.6% to 82.0%, and the current model had a better prognostic value than TNM-classification with a lower Akaike information criterion (AIC) value of 166.54. In vitro experiments suggested that Wnt3a could regulate EMT-related proteins and promotes in vitro invasion and migration abilities. Conclusions: Wnt3a could regulate EMT-related proteins and promote the migration and invasion process of lung adenocarcinoma. Although its value as an independent prognostic factor was limited, the combined model suggested good prognostic performance for lung adenocarcinoma patients.
journal_name
J Cancerjournal_title
Journal of Cancerauthors
Xu J,Lv W,Hu Y,Wang L,Wang Y,Cao J,Hu Jdoi
10.7150/jca.18560subject
Has Abstractpub_date
2017-08-02 00:00:00pages
2523-2531issue
13issn
1837-9664pii
jcav08p2523journal_volume
8pub_type
杂志文章abstract::Objective: The purpose of this study was to evaluate the efficacy and feasibility of the R.E.N.A.L. Nephrometry Score to postoperatively predict high-grade clear-cell renal carcinoma (ccRCC). Methods: The study included 288 patients diagnosed with ccRCC who had complete CT/CTA data and R.E.N.A.L. Nephrometry Scores an...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.21189
更新日期:2017-10-17 00:00:00
abstract::Hepatocellular carcinoma (HCC) cells exhibit multidrug resistance (MDR), but the underlying mechanisms remain unclear. Cancer cells that overexpress telomerase are resistant to chemotherapeutic drugs. This study aimed to determine the effects of mitochondrial translocation of telomerase on MDR in HCC cells. HepG2 cell...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.10419
更新日期:2015-01-05 00:00:00
abstract::Purpose: To design an alternative workflow for cancer-risk assessment to predict distant metastasis (DM) of nasopharyngeal carcinoma (NPC). Methods: We enrolled 234 patients with non-disseminated NPC and a family history of cancer who underwent intensity-modulated radiotherapy and concurrent chemo-radiotherapy with/wi...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.19611
更新日期:2017-07-20 00:00:00
abstract::Circular RNAs (circRNAs), as a type of tissue specific RNA with more stable structure than linear RNAs, was poorly understood on its correlation with gastric cancer (GC). In this review, we outline the synthesis and characteristics of circRNAs and generalize their categories and functions. Through comprehensive analys...
journal_title:Journal of Cancer
pub_type: 杂志文章,评审
doi:10.7150/jca.32927
更新日期:2019-10-15 00:00:00
abstract:PURPOSE:Our study is designed to develop and certify a promising prognostic signature for hepatocellular carcinoma (HCC). Materials and methods: We retrospectively analyzed mRNA expression profiles and clinicopathological data fetched from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets. We fo...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.44573
更新日期:2020-07-31 00:00:00
abstract:OBJECTIVE(S):To assess skeletal wide fracture location and time of fracture after cancer treatment Study Design: One hundred thirty-nine women diagnosed with breast or gynecologic cancer between 2003 and 2012 that subsequently had a radiologic diagnosis of fracture were identified retrospectively using electronic medic...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.10288
更新日期:2015-01-01 00:00:00
abstract::Tumor growth depends on angiogenesis, the growth of new blood vessels. Complement factor H (CFH) is a plasma glycoprotein that functions as a regulator of the complement system. The aim of this study is to delineate the role of CFH in angiogenesis. A conditional null allele of the Cfh gene was generated in C57BL/6J mi...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.19452
更新日期:2017-07-15 00:00:00
abstract::EGFR mutation of Non-small cell lung cancers (NSCLC) was predominantly seen in Asian population and it was considered as a predictor of responsiveness. Eendothelial nitric oxide synthase (eNOS) plays a vital role in chronic inflammation and carcinogenesis. In this study, we aimed to explore the association between the...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.25824
更新日期:2018-06-15 00:00:00
abstract::MPHOSPH1, which is one of the kinesin superfamily proteins, has been reported to play an essential role in the carcinogenesis and progression of several kinds of cancers. MPHOSPH1 has also been suggested to be involved in STAT3 phosphorylation in hepatocellular carcinoma. However, the biological behavior of MPHOSPH1 i...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.25279
更新日期:2018-10-31 00:00:00
abstract::Clinical studies have confirmed epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors (TKIs) used in lung cancer patients with EGFR mutations can obtain a better result, but still part of the patients with poor efficacy. EGFR mutation is highly related to female, nonsmoking and adenocarcinoma. Thus, we hy...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.25679
更新日期:2018-07-30 00:00:00
abstract::Endometrial and ovarian cancers are two most common cancers affecting women in their post-menopausal years. To date, there are no simple biochemical tests to detect these cancers at early stages. Our previous study has demonstrated that the activity of total proprotein convertases (PCs) is significantly increased in u...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.16331
更新日期:2016-08-12 00:00:00
abstract::Little is known about the possible oncogenic roles of genes encoding for the phosphatidylinositol 4-kinases, a family of enzymes that regulate an early step in phosphoinositide signalling. To address this issue, the mutational status of all four human phosphatidylinositol 4-kinases genes was analyzed across 852 breast...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.9794
更新日期:2014-10-28 00:00:00
abstract:OBJECTIVES:To study whether radical nephrectomy (RN) with lymph node dissection (LND) can benefit pT3 renal cell carcinoma (RCC) patients versus no LND under the 2018 American Joint Committee on Cancer TNM classification system. Subjects/Patients and Methods: We performed a retrospective cohort study of clinicopatholog...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.30375
更新日期:2019-05-26 00:00:00
abstract::Purpose: We retrospectively collected consecutive survival data of lung adenosquamous cell carcinoma (ASC) patients with brain metastasis (BM) in our institute and discussed the factors related to prognosis of these patients. Patients and Methods: A total of 42 patients diagnosed as lung ASC with BM between July 1, 20...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.27441
更新日期:2018-09-08 00:00:00
abstract::Objective: Chemotherapy is a major therapeutic method for bladder urothelial carcinoma (BUC), which can effectively improve the prognosis of BUC patients, but the chemoresistance often leads to chemotherapy failure. This study will research the regulatory roles and molecular mechanism of miR-101-3p in BUC chemoresista...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.33117
更新日期:2019-06-02 00:00:00
abstract::Triple negative breast cancer (TNBC) is characterized by distinctive biological features that confer an aggressive clinical behavior. In TNBC patients, the absence of well-defined driver pathways such as hormonal receptor expression or hyperactivation of the human epidermal growth factor receptor 2 (HER2) significantl...
journal_title:Journal of Cancer
pub_type: 杂志文章,评审
doi:10.7150/jca.35109
更新日期:2019-10-12 00:00:00
abstract::Traditional chemotherapy and radiotherapy for cancer treatment face serious challenges such as drug resistance and toxic side effects. Complementary / Alternative medicine is increasingly being practiced worldwide due to its safety beneficial therapeutic effects. We hypothesized that a super combination (SC) of known ...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.7235
更新日期:2013-11-14 00:00:00
abstract::Purpose Neuroblastoma is an embryonic solid tumor derived from the progenitors of the sympathetic nervous system. More than half of the patients developed metastatic disease at the time of initial diagnosis and had poor outcome with current therapeutic approaches. In recent years, some obligate and facultative anaerob...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.18776
更新日期:2017-06-03 00:00:00
abstract::The authors retrospectively examined the contralateral prophylactic mastectomy (CPM) rate among 100 women with ductal carcinoma in situ who are BRCA negative. Of 100 women with ductal carcinoma in situ, 31 elected contralateral prophylactic mastectomy (CPM). Factors associated with increased likelihood of undergoing c...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.11710
更新日期:2015-05-23 00:00:00
abstract::Ovarian cancer is a highly lethal malignancy in the female reproductive system. Platinum drugs, represented by cisplatin, are the first-line chemotherapeutic agents for treatment of various malignancies including ovarian cancer, but drug resistance leads to chemotherapy failure. MicroRNAs emerged as promising molecule...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.41135
更新日期:2020-03-13 00:00:00
abstract:BACKGROUND:The 2010 guidelines by ASCO-CAP have mandated that breast cancer specimens with ≥1% positively staining cells by immunohistochemistry should be considered Estrogen Receptor (ER) positive. This has led to a subclass of low-ER positive (1-10%) breast cancers. We have examined the biology and clinical behavior ...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.7668
更新日期:2014-01-23 00:00:00
abstract::Liver kinase B1 (LKB1) is a critical tumor suppressor that is frequently mutated in human cancers. LKB1 has serine/threonine protein kinase activity, which regulates gene expression by phosphorylation of Yes-Associated protein (YAP). The phosphorylation-dependent YAP shuttling is critically important intracellular mec...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.33237
更新日期:2019-06-09 00:00:00
abstract::Cachexia is a metabolic mutiny that directly reduces life expectancy in chronic conditions such as cancer. The underlying mechanisms associated with cachexia involve inflammation, metabolism, and anorexia. Therefore, the need to identify cachexia biomarkers is warranted to better understand catabolism change and asses...
journal_title:Journal of Cancer
pub_type: 杂志文章,评审
doi:10.7150/jca.50376
更新日期:2021-01-01 00:00:00
abstract::Myeloid-derived suppressor cells (MDSCs) have strong immunosuppressive functions and contribute to the formation of the tumor microenvironment. Long non-coding (Lnc) RNAs are highly important factors associated with tumors and may be used as markers for tumor diagnosis, which is valuable for targeted therapy. LncRNA M...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.24796
更新日期:2018-06-14 00:00:00
abstract::Melanoma is the most aggressive and treatment-resistant form of skin cancer. Curcumol is a Chinese medicinal herb traditionally used as a cancer remedy. However, the molecular mechanisms underlying the anticancer activity of curcumol in melanoma remains largely unknown. In the present study, we observed that Curcumol ...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.38624
更新日期:2020-01-14 00:00:00
abstract::Objective: Liposarcoma is a mesenchymal malignant tumor characterized by adipocyte differentiation which is divided into four subtypes with different prognosis. Accurate histopathological diagnosis is essential for precise treatment. Perilipins, including PLIN1, PLIN2, PLIN3, PLIN4, PLIN5, is a family of lipid droplet...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.41736
更新日期:2020-04-07 00:00:00
abstract::Objective: To enquire into the clinical significance and potential molecular mechanism of microRNA (miRNA)-196b-5p in hepatocellular carcinoma (HCC). Methods: Quantitative reverse transcription and polymerase chain reaction (qRT-PCR) were utilized to examine miR-196b-5p expression level in 67 HCC paraffin embedded tis...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.29293
更新日期:2019-08-29 00:00:00
abstract::Background: Given recent results indicating that diminished LKB1 expression in laryngeal cancer correlates with shorter survival. We aim to perform an analysis estimate the role of decreased liver kinase B1(LKB1) and in the prognostication of human laryngeal squamous cell carcinoma (LSCC). Methods: We conducted a retr...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.20535
更新日期:2017-09-30 00:00:00
abstract::Red blood cell distribution width (RDW) has been recently demonstrated to be a predictor of inflammation. High pretreatment RDW level is associated with poor survival outcomes in various malignancies, although the results are controversial. We aimed to investigate the prognostic role of RDW. A systematic literature se...
journal_title:Journal of Cancer
pub_type: 杂志文章,评审
doi:10.7150/jca.31598
更新日期:2019-07-10 00:00:00
abstract::Non epithelial ovarian tumors represent a small group of rare gynecological cancers but they have a high incidence in young childbearing women. The recent years fertility preservation surgeries have become a common practice in specific ovarian malignancies when the patients wish to maintain their fertility. Specific t...
journal_title:Journal of Cancer
pub_type: 杂志文章,评审
doi:10.7150/jca.26674
更新日期:2018-11-24 00:00:00