Simultaneous inhibition of cell-cycle, proliferation, survival, metastatic pathways and induction of apoptosis in breast cancer cells by a phytochemical super-cocktail: genes that underpin its mode of action.

Abstract:

:Traditional chemotherapy and radiotherapy for cancer treatment face serious challenges such as drug resistance and toxic side effects. Complementary / Alternative medicine is increasingly being practiced worldwide due to its safety beneficial therapeutic effects. We hypothesized that a super combination (SC) of known phytochemicals used at bioavailable levels could induce 100% killing of breast cancer (BC) cells without toxic effects on normal cells and that microarray analysis would identify potential genes for targeted therapy of BC. Mesenchymal Stems cells (MSC, control) and two BC cell lines were treated with six well established pro-apoptotic phytochemicals individually and in combination (super cocktail), at bioavailable levels. The compounds were ineffective individually. In combination, they significantly suppressed BC cell proliferation (>80%), inhibited migration and invasion, caused cell cycle arrest and induced apoptosis resulting in 100% cell death. However, there were no deleterious effects on MSC cells used as control. Furthermore, the SC down-regulated the expression of PCNA, Rb, CDK4, BcL-2, SVV, and CD44 (metastasis inducing stem cell factor) in the BC cell lines. Microarray analysis revealed several differentially expressed key genes (PCNA, Rb, CDK4, Bcl-2, SVV, P53 and CD44) underpinning SC-promoted BC cell death and motility. Four unique genes were highly up-regulated (ARC, GADD45B, MYLIP and CDKN1C). This investigation indicates the potential for development of a highly effective phytochemical combination for breast cancer chemoprevention / chemotherapy. The novel over-expressed genes hold the potential for development as markers to follow efficacy of therapy.

journal_name

J Cancer

journal_title

Journal of Cancer

authors

Ouhtit A,Gaur RL,Abdraboh M,Ireland SK,Rao PN,Raj SG,Al-Riyami H,Shanmuganathan S,Gupta I,Murthy SN,Hollenbach A,Raj MH

doi

10.7150/jca.7235

subject

Has Abstract

pub_date

2013-11-14 00:00:00

pages

703-15

issue

9

issn

1837-9664

pii

jcav04p0703

journal_volume

4

pub_type

杂志文章
  • Incidence and Impact of Lymph Node Metastases in Advanced Ovarian Cancer: Implications for Surgical Treatment.

    abstract::Background: This study aimed to clarify the impact of node involvement (affected to resected nodes) in optimally cytoreduced (residual tumour ≤1cm) stage IIIC/IV ovarian cancer. Methods: 108 consecutive patients with primary stage IIIC/IV ovarian cancer underwent stage-related surgery and got adjuvant platinum-based c...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.15644

    authors: Bachmann C,Bachmann R,Fend F,Wallwiener D

    更新日期:2016-11-09 00:00:00

  • Methylation patterns of RASA3 associated with clinicopathological factors in hepatocellular carcinoma.

    abstract::Hepatocellular carcinoma (HCC) is the sixth most common tumor worldwide. The relationship between the gene methylation accumulation and HCC has been widely studied. In our study, we used the Sequenom EpiTYPER assay to investigate the methylation levels of the RASA3 in 164 HCC samples and paired adjacent non-cancerous ...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.24567

    authors: Lin H,Fan X,He L,Zhou D

    更新日期:2018-05-25 00:00:00

  • GV1001 inhibits cell viability and induces apoptosis in castration-resistant prostate cancer cells through the AKT/NF-κB/VEGF pathway.

    abstract::Purpose: We examined the effect of GV1001 in castration castration-resistant prostate cancer (CRPC) cell growth and invasion and explored the potential molecular mechanisms of action. Materials and Methods: The in vitro anti-cancer effects of GV1001 in CRPC cells were examined using cell viability assay, TUNEL assay, ...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.34859

    authors: Park YH,Jung AR,Kim GE,Kim MY,Sung JW,Shin D,Cho HJ,Ha US,Hong SH,Kim SW,Lee JY

    更新日期:2019-10-17 00:00:00

  • Risk of immune-related colitis with PD-1/PD-L1 inhibitors vs chemotherapy in solid tumors: systems assessment.

    abstract::Background: We performed a meta-analysis to evaluate the risk of immune-related colitis associated with PD1/PD-L1 inhibitors as compared to chemotherapy in solid tumor patients. Methods: Eligible studies were identified through a comprehensive search of multiple databases and included solid tumor patients in randomize...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.24200

    authors: Su Q,Zhang X,Shen X,Hou Y,Sun Z,Gao ZH

    更新日期:2018-04-12 00:00:00

  • Fibroblast growth factor receptor signaling as therapeutic targets in female reproductive system cancers.

    abstract::Ovarian cancer, cervical cancer and endometrial cancer are three relatively common malignant cancers of the female reproductive system. Despite improvements in female genital tract cancer detection and development of new therapeutic approaches, there are still poor prognoses and some do not respond to therapeutic patt...

    journal_title:Journal of Cancer

    pub_type: 杂志文章,评审

    doi:10.7150/jca.44727

    authors: Zhu DL,Tuo XM,Rong Y,Zhang K,Guo Y

    更新日期:2020-10-21 00:00:00

  • LPS and IL-8 activated umbilical cord blood-derived neutrophils inhibit the progression of ovarian cancer.

    abstract::Background: Immunotherapy including immune checkpoint blockade, cancer vaccines, and adoptive cell therapy. However, no immune therapies support ovarian cancer. It is not clear whether the neutrophils, the component of the immune system derived from umbilical cord blood play a role in inhibiting the progression of ova...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.41035

    authors: Liu Q,Yang W,Luo N,Liu J,Wu Y,Ding J,Li C,Cheng Z

    更新日期:2020-05-18 00:00:00

  • MCMs expression in lung cancer: implication of prognostic significance.

    abstract::Minichromosome Maintenance (MCM) proteins play essential roles in various cancers. We previously reported that MCM7 could be a prognostic biomarker in non-small cell lung cancer (NSCLC). The purpose of current study is to explore roles of other MCM proteins in NSCLC and their correlation with clinico-pathologic parame...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.20777

    authors: Liu YZ,Wang BS,Jiang YY,Cao J,Hao JJ,Zhang Y,Xu X,Cai Y,Wang MR

    更新日期:2017-10-09 00:00:00

  • Significant prognostic impact of chemoradiotherapy-induced hemoglobin decrease on treatment outcomes of nasopharyngeal carcinoma.

    abstract:PURPOSE:To investigate prognostic impact of chemoradiotherapy-induced hemoglobin (Hb) decrease on treatment outcomes of endemic nasopharyngeal carcinoma (NPC). MATERIALS AND METHODS:Eight hundred and fifteen non-metastatic NPC, receiving neoadjuvant chemotherapy followed by radiotherapy (NACT+RT group) or concomitant ...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.11403

    authors: Liang XX,Li Q,Su Z,Lan XW,Ouyang PY,Mao YP,Shi DB,Deng WG,Cheng ZB,Wang SY,Xie FY

    更新日期:2015-04-02 00:00:00

  • Gene Signal Distribution and HER2 Amplification in Gastroesophageal Cancer.

    abstract::Background: HER2 serves as an important therapeutic target in gastroesophageal cancer. Differences in HER2 gene signal distribution patterns can be observed at the tissue level, but how it influences the HER2 amplification status has not been studied so far. Here, we investigated the link between HER2 amplification an...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.17878

    authors: Jørgensen JT,Nielsen KB,Kjærsgaard G,Jepsen A,Mollerup J

    更新日期:2017-06-01 00:00:00

  • Total PC Activity Is Increased in Uterine Lavage of Post-Menopausal Endometrial but Not Ovarian Cancer Patients.

    abstract::Endometrial and ovarian cancers are two most common cancers affecting women in their post-menopausal years. To date, there are no simple biochemical tests to detect these cancers at early stages. Our previous study has demonstrated that the activity of total proprotein convertases (PCs) is significantly increased in u...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.16331

    authors: Heng S,Stephens AN,Jobling TW,Nie G

    更新日期:2016-08-12 00:00:00

  • Efficacy of Cabazitaxel Treatment in Metastatic Castration Resistant Prostate Cancer in Second and Later Lines. An Experience from Two German Centers.

    abstract::Purpose: Several new treatment options for patients with metastatic castration resistant prostate cancer (mCRPC) have been approved within the last years - among them cabazitaxel (CAB), abiraterone acetate, enzalutamide, and radium-223. The aim of this study was to assess factors predictive for efficacy of CAB. Method...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.17644

    authors: Zschäbitz S,Vallet S,Hadaschik B,Debatin D,Fuxius S,Karcher A,Pahernik S,Spath C,Duensing S,Jäger D,Hohenfellner M,Grüllich C

    更新日期:2017-02-11 00:00:00

  • Whole-exome Sequencing of Prostate Cancer in Sardinian Identify Recurrent UDP-glucuronosyltransferase Amplifications.

    abstract::Globally, prostate cancer is the third most common cancer in the world, and the second most common cancer in men. However, rates for incidence and mortality vary considerably with race, ethnicity, and geography. Over 97 significantly mutated genes that have been identified in prostate cancer; however, a lack of genomi...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.48433

    authors: Zeng T,Fedeli MA,Tanda F,Wang Y,Yang D,Xue B,Jia L,Palmieri G,Sechi LA,Kelvin DJ

    更新日期:2021-01-01 00:00:00

  • Plasmacytoid Dendritic Cells and ICOS+ Regulatory T Cells Predict Poor Prognosis in Gastric Cancer: A Pilot Study.

    abstract::Background: Regulatory T cells (Tregs) and plasmacytoid dendritic cells (pDCs) are the main immunosuppressive cells in tumor microenvironment of gastric cancer (GC). In this prospective study, the association of prognosis with Tregs subsets and pDCs were further analyzed. Methods: pDCs, Tregs population and its expres...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.34826

    authors: Liu X,Yu H,Yan C,Mei Y,Lin C,Hong Y,Lin X,Zhang Q,Yu J

    更新日期:2019-10-22 00:00:00

  • Ethanol Enhances Estrogen Mediated Angiogenesis in Breast Cancer.

    abstract::Angiogenesis, a highly regulated process, is exploited by tumors like breast cancer to ensure a constant supply of oxygen and nutrients and is key for tumor survival and progression. Estrogen and alcohol independently have been observed to contribute to angiogenesis in breast cancer but their combinatorial effects hav...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.25581

    authors: Maniyar R,Chakraborty S,Suriano R

    更新日期:2018-10-06 00:00:00

  • Treatment of Neuroblastoma with an Engineered "Obligate" Anaerobic Salmonella typhimurium Strain YB1.

    abstract::Purpose Neuroblastoma is an embryonic solid tumor derived from the progenitors of the sympathetic nervous system. More than half of the patients developed metastatic disease at the time of initial diagnosis and had poor outcome with current therapeutic approaches. In recent years, some obligate and facultative anaerob...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.18776

    authors: Ning BT,Yu B,Chan S,Chan JL,Huang JD,Chan GC

    更新日期:2017-06-03 00:00:00

  • Tripartite motif-containing 15 overexpression in non-small cell lung cancer is associated with poor patient prognoses.

    abstract::Purpose: This study aimed to comprehensively investigate the differential expression and prognostic indicators of the tripartite motif-containing (TRIM) gene family in non-small cell lung cancer (NSCLC). Methods: The Cancer Genome Atlas (TCGA) Research Network and three datasets from Gene Expression Omnibus (GEO) data...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.27856

    authors: Han X,Huang C,Qu X,Liu S,Yang X,Wang Y,Bie F,Liu Q,Du J

    更新日期:2019-01-29 00:00:00

  • Novel Metabolomics Serum Biomarkers for Pancreatic Ductal Adenocarcinoma by the Comparison of Pre-, Postoperative and Normal Samples.

    abstract::Background: Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive human malignancies. The metabolomic approaches are developed to discover the novel biomarkers of PDAC. Methods: 550 preoperative, postoperative PDAC and normal controls (NCs) serums were employed to characterize metabolic alterations in ...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.41250

    authors: Zhang X,Shi X,Lu X,Li Y,Zhan C,Akhtar ML,Yang L,Bai Y,Zhao J,Wang Y,Yao Y,Li Y,Nie H

    更新日期:2020-05-19 00:00:00

  • Progression-free Survival Decreases with Each Subsequent Therapy in Patients Presenting for Phase I Clinical Trials.

    abstract:BACKGROUND:There is often a finite progression-free interval of time between one systemic therapy and the next when treating patients with advanced cancer. While it appears that progression-free survival (PFS) between systemic therapies tends to get shorter for a number of factors, there has not been a formal evaluatio...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.3.7

    authors: Bailey CH,Jameson G,Sima C,Fleck S,White E,Von Hoff DD,Weiss GJ

    更新日期:2012-01-01 00:00:00

  • Histone deacetylase inhibition up-regulates MHC class I to facilitate cytotoxic T lymphocyte-mediated tumor cell killing in glioma cells.

    abstract::Background: Immune cells recognize tumor antigens presented on major histocompatibility complex class I (MHC-I) molecule. Increase of MHC-I molecular expression makes tumor cells more susceptible to lysis by immune cells. Methods: Tumor lysate vaccine was prepared to damage glioma cells including cell lines and primar...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.34471

    authors: Sun T,Li Y,Yang W,Wu H,Li X,Huang Y,Zhou Y,Du Z

    更新日期:2019-09-07 00:00:00

  • Can Patients with Pancreatic Cancer and Liver Metastases Obtain Survival Benefit from Surgery? A Population-Based Study.

    abstract::Background: Surgery for pancreatic cancer with liver metastases (PCL) is not recommended in the international guidelines, and investigation of its clinical significance in patients with PCL is very limited. This study explored whether surgery, especially synchronous resection of the primary tumor and liver metastases ...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.51218

    authors: Su BB,Bai DS,Yu JQ,Zhang C,Jin SJ,Zhou BH,Jiang GQ

    更新日期:2021-01-01 00:00:00

  • Cucurbitacin E inhibits cellular proliferation and enhances the chemo-response in gastric cancer by suppressing AKt activation.

    abstract::Background: The incidence and mortality rate of gastric cancer has markedly declined over the past few decades, due to the progress and advances in the development of diagnostic and treatment regimens. However, there is still a large portion of patients who are first diagnosed during the advanced stage of gastric canc...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.31303

    authors: Si W,Lyu J,Liu Z,Wang C,Huang J,Jiang L,Ma T

    更新日期:2019-10-06 00:00:00

  • Stromal mesenchymal stem cells facilitate pancreatic cancer progression by regulating specific secretory molecules through mutual cellular interaction.

    abstract::Pancreatic ductal adenocarcinoma (PDAC) is currently one of the most intractable malignancies with a typical scirrhous pattern in histology. Due to its abundant tumor stroma and scant vascularization, chemotherapeutic agents are considered inefficiently permeable to cancer nests, making it highly difficult to cure the...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.24415

    authors: Saito K,Sakaguchi M,Maruyama S,Iioka H,Putranto EW,Sumardika IW,Tomonobu N,Kawasaki T,Homma K,Kondo E

    更新日期:2018-07-30 00:00:00

  • Human Stromal Cells in the Peripheral Zone of the Prostate Promote Tumorigenesis of Prostatic Cancer Stem Cells through Up-regulation of C-Kit Expression.

    abstract:OBJECTIVE:Most prostate cancers originate from the prostatic peripheral zone (PZ). We tested the hypothesis that the stromal cells from PZ and transitional zone (TZ) have differential effects on the ability of tumorigenesis. METHODS:Stromal cells isolated from the PZ and TZ of normal human prostates mixed with DU145 c...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.9961

    authors: Peng Y,Chen Q,Gu M,Chen Y,Zhang M,Zhou J,Wang H,Gao Y,Li W,Wang Z,Cai Z

    更新日期:2015-07-03 00:00:00

  • Secretory Clusterin Mediates Oxaliplatin Resistance via the Gadd45a/PI3K/Akt Signaling Pathway in Hepatocellular Carcinoma.

    abstract::Purpose: Systemic therapy has often been used for patients with advanced hepatocellular carcinoma (HCC). However, due to drug resistance, the use of cytotoxic chemotherapy in the treatment of patients with advanced HCC has typically demonstrated low response rates. Secretory clusterin (sCLU) is expressed in aggressive...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.23849

    authors: Wang X,Zou F,Zhong J,Yue L,Wang F,Wei H,Yang G,Jin T,Dong X,Li J,Xiu P

    更新日期:2018-04-06 00:00:00

  • Anticancer Properties of Fenofibrate: A Repurposing Use.

    abstract::Cancer is a leading cause of death throughout the world, and cancer therapy remains a big medical challenge in terms of both its therapeutic efficacy and safety. Therefore, to find out a safe anticancer drug has been long goal for oncologist and medical scientists. Among clinically used medicines with no or little tox...

    journal_title:Journal of Cancer

    pub_type: 杂志文章,评审

    doi:10.7150/jca.24488

    authors: Lian X,Wang G,Zhou H,Zheng Z,Fu Y,Cai L

    更新日期:2018-04-06 00:00:00

  • TGF-β1-Induced Upregulation of MALAT1 Promotes Kazakh's Esophageal Squamous Cell Carcinoma Invasion by EMT.

    abstract::Transforming growth factor β1 (TGF-β1) plays an important role in tumor initiation and development by inducing epithelial-mesenchymal Transition (EMT). Metastasis-Associated Lung Adenocarcinoma Transcript 1 (MALAT1) is a long noncoding RNA (lncRNA) that contributes to the invasion and metastasis of tumors, including e...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.48426

    authors: Liu Q,Zheng S,Chen Y,Liu T,Han X,Zhang X,Shen T,Lu X

    更新日期:2020-10-04 00:00:00

  • The PARP-1 inhibitor Olaparib suppresses BRCA1 protein levels, increases apoptosis and causes radiation hypersensitivity in BRCA1+/- lymphoblastoid cells.

    abstract::The use of polyADPribose polymerase inhibitors in cancer treatment provides a unique opportunity to target DNA repair processes in cancer cells while leaving normal tissue intact. The PARP-1 enzyme repairs DNA single strand breaks (SSB). Therefore PARP-1 inhibition in BRCA1 negative cancers results in the formation of...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.21338

    authors: Bourton EC,Ahorner PA,Plowman PN,Zahir SA,Al-Ali H,Parris CN

    更新日期:2017-10-23 00:00:00

  • Effect of TTLL6 expression on CDDP sensitivity of EC109/CDDP cells in hypoxia/acidosis microenvironment.

    abstract::Multidrug resistance is a major obstacle to the effective treatment of esophageal carcinoma. It occurs more readily in hypoxia and acidosis microenvironment. TTLL6 is one of Tubulin tyrosine ligase-like family members. In this study, the effect of TTLL6 on the regulation of cisplatin (CDDP) sensitivity was evaluated i...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.47694

    authors: Qiu Y,Wu C,Li J,Tang M,Zhang S,Jing T,Liao Y,Wang H

    更新日期:2020-09-30 00:00:00

  • Role of Chemokines in Non-Small Cell Lung Cancer: Angiogenesis and Inflammation.

    abstract::Non-small cell lung cancer (NSCLC) is one of the most common types of aggressive cancer. The tumor tissue, which shows an active angiogenesis, is composed of neoplastic and stromal cells, and an abundant inflammatory infiltrate. Angiogenesis is important to support tumor growth, while infiltrating cells contribute to ...

    journal_title:Journal of Cancer

    pub_type: 杂志文章,评审

    doi:10.7150/jca.12286

    authors: Rivas-Fuentes S,Salgado-Aguayo A,Pertuz Belloso S,Gorocica Rosete P,Alvarado-Vásquez N,Aquino-Jarquin G

    更新日期:2015-08-07 00:00:00

  • Survival benefit of adjuvant chemotherapy for patients with poorly differentiated stage IIA colon cancer.

    abstract::Background: The effect of adjuvant chemotherapy on stage II colon cancer remains constantly controversial. Recently, however, several studies have reported the definite survival benefit of adjuvant chemotherapy (ACT) in T4 disease (stage IIB and IIC) among stage II colon cancer patients. The following study investigat...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.28917

    authors: Liu Q,Luo D,An H,Zhang S,Cai S,Li Q,Li X

    更新日期:2019-01-29 00:00:00