The iron distribution and magnetic properties of schistosome eggshells: implications for improved diagnostics.


BACKGROUND:Schistosoma mansoni and Schistosoma japonicum are the most frequent causative agents of human intestinal schistosomiasis. Approximately 200 million people in the world are infected with schistosomes. Diagnosis of schistosomiasis is often difficult. High percentages of low level infections are missed in routine fecal smear analysis and current diagnostic methodologies are inadequate to monitor the progress of parasite control, especially in areas with low transmission. Improved diagnostic methods are urgently needed to evaluate the success of elimination programs. Recently, a magnetic fractionation method for isolation of parasite eggs from feces was described, which uses magnetic microspheres to form parasite egg - magnetic microsphere conjugates. This approach enables screening of larger sample volumes and thus increased diagnostic sensitivity. The mechanism of formation of the conjugates remains unexplained and may either be related to specific surface characteristics of eggs and microspheres or to their magnetic properties. METHODS/PRINCIPAL FINDINGS:Here, we investigated iron localization in parasite eggs, specifically in the eggshells. We determined the magnetic properties of the eggs, studied the motion of eggs and egg-microsphere conjugates in magnetic fields and determined species specific affinity of parasite eggs to magnetic microspheres. Our study shows that iron is predominantly localized in pores in the eggshell. Parasite eggs showed distinct paramagnetic behaviour but they did not move in a magnetic field. Magnetic microspheres spontaneously bound to parasite eggs without the presence of a magnetic field. S. japonicum eggs had a significantly higher affinity to bind microspheres than S. mansoni eggs. CONCLUSIONS/SIGNIFICANCE:Our results suggest that the interaction of magnetic microspheres and parasite eggs is unlikely to be magnetic in origin. Instead, the filamentous surface of the eggshells may be important in facilitating the binding. Modification of microsphere surface properties may therefore be a way to optimize magnetic fractionation of parasite eggs.


PLoS Negl Trop Dis


Karl S,Gutiérrez L,Lucyk-Maurer R,Kerr R,Candido RR,Toh SQ,Saunders M,Shaw JA,Suvorova A,Hofmann A,House MJ,Woodward RC,Graeff-Teixera C,St Pierre TG,Jones MK




Has Abstract


2013-05-16 00:00:00














  • Molecular investigations of a locally acquired case of melioidosis in Southern AZ, USA.

    abstract::Melioidosis is caused by Burkholderia pseudomallei, a Gram-negative bacillus, primarily found in soils in Southeast Asia and northern Australia. A recent case of melioidosis in non-endemic Arizona was determined to be the result of locally acquired infection, as the patient had no travel history to endemic regions and...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Engelthaler DM,Bowers J,Schupp JA,Pearson T,Ginther J,Hornstra HM,Dale J,Stewart T,Sunenshine R,Waddell V,Levy C,Gillece J,Price LB,Contente T,Beckstrom-Sternberg SM,Blaney DD,Wagner DM,Mayo M,Currie BJ,Keim P,Tua

    更新日期:2011-10-01 00:00:00

  • Identification of anisomycin, prodigiosin and obatoclax as compounds with broad-spectrum anti-parasitic activity.

    abstract::Parasitic infections are a major source of human suffering, mortality, and economic loss, but drug development for these diseases has been stymied by the significant expense involved in bringing a drug though clinical trials and to market. Identification of single compounds active against multiple parasitic pathogens ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Ehrenkaufer G,Li P,Stebbins EE,Kangussu-Marcolino MM,Debnath A,White CV,Moser MS,DeRisi J,Gisselberg J,Yeh E,Wang SC,Company AH,Monti L,Caffrey CR,Huston CD,Wang B,Singh U

    更新日期:2020-03-20 00:00:00

  • Broad diversity of Mycobacterium tuberculosis complex strains isolated from humans and cattle in Northern Algeria suggests a zoonotic transmission cycle.

    abstract::Mycobacterium tuberculosis complex (MTBC) comprises closely related species responsible for human and animal tuberculosis (TB). Efficient species determination is useful for epidemiological purposes, especially for the elucidation of the zoonotic contribution. In Algeria, data on MTBC genotypes are largely unknown. In...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Damene H,Tahir D,Diels M,Berber A,Sahraoui N,Rigouts L

    更新日期:2020-11-30 00:00:00

  • Targeting trachoma control through risk mapping: the example of Southern Sudan.

    abstract:BACKGROUND:Trachoma is a major cause of blindness in Southern Sudan. Its distribution has only been partially established and many communities in need of intervention have therefore not been identified or targeted. The present study aimed to develop a tool to improve targeting of survey and control activities. METHODS...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Clements AC,Kur LW,Gatpan G,Ngondi JM,Emerson PM,Lado M,Sabasio A,Kolaczinski JH

    更新日期:2010-08-17 00:00:00

  • High Resolution Melting Analysis Targeting hsp70 as a Fast and Efficient Method for the Discrimination of Leishmania Species.

    abstract:BACKGROUND:Protozoan parasites of the genus Leishmania cause a large spectrum of clinical manifestations known as Leishmaniases. These diseases are increasingly important public health problems in many countries both within and outside endemic regions. Thus, an accurate differential diagnosis is extremely relevant for ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Zampieri RA,Laranjeira-Silva MF,Muxel SM,Stocco de Lima AC,Shaw JJ,Floeter-Winter LM

    更新日期:2016-02-29 00:00:00

  • Risk of disability among adult leprosy cases and determinants of delay in diagnosis in five states of India: A case-control study.

    abstract:INTRODUCTION:A high proportion of grade 2 disability (visible deformity) is indicative of delay in detection of leprosy and leprosy is one of the major causes of preventable disability. We conducted this study to determine the risk factors associated with disability (G2D and G1D) among adult new leprosy cases and to me...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,多中心研究


    authors: Srinivas G,Muthuvel T,Lal V,Vaikundanathan K,Schwienhorst-Stich EM,Kasang C

    更新日期:2019-06-27 00:00:00

  • Engineered action at a distance: Blood-meal-inducible paralysis in Aedes aegypti.

    abstract:BACKGROUND:Population suppression through mass-release of Aedes aegypti males carrying dominant-lethal transgenes has been demonstrated in the field. Where population dynamics show negative density-dependence, suppression can be enhanced if lethality occurs after the density-dependent (i.e. larval) stage. Existing mole...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Haghighat-Khah RE,Harvey-Samuel T,Basu S,StJohn O,Scaife S,Verkuijl S,Lovett E,Alphey L

    更新日期:2019-09-03 00:00:00

  • Epidemiology of Chikungunya Virus Outbreaks in Guadeloupe and Martinique, 2014: An Observational Study in Volunteer Blood Donors.

    abstract:BACKGROUND:During Dec-2013, a chikungunya virus (CHIKV) outbreak was first detected in the French-West Indies. Subsequently, the virus dispersed to other Caribbean islands, continental America and many islands in the Pacific Ocean. Previous estimates of the attack rate were based on declaration of clinically suspected ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Gallian P,Leparc-Goffart I,Richard P,Maire F,Flusin O,Djoudi R,Chiaroni J,Charrel R,Tiberghien P,de Lamballerie X

    更新日期:2017-01-12 00:00:00

  • External quality assessment study for ebolavirus PCR-diagnostic promotes international preparedness during the 2014 - 2016 Ebola outbreak in West Africa.

    abstract::During the recent Ebola outbreak in West Africa several international mobile laboratories were deployed to the mainly affected countries Guinea, Sierra Leone and Liberia to provide ebolavirus diagnostic capacity. Additionally, imported cases and small outbreaks in other countries required global preparedness for Ebola...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,多中心研究


    authors: Ellerbrok H,Jacobsen S,Patel P,Rieger T,Eickmann M,Becker S,Günther S,Naidoo D,Schrick L,Keeren K,Targosz A,Teichmann A,Formenty P,Niedrig M

    更新日期:2017-05-01 00:00:00

  • Dried-blood spots: a cost-effective field method for the detection of Chikungunya virus circulation in remote areas.

    abstract:BACKGROUND:In 2005, there were outbreaks of febrile polyarthritis due to Chikungunya virus (CHIKV) in the Comoros Islands. CHIKV then spread to other islands in the Indian Ocean: La Réunion, Mauritius, Seychelles and Madagascar. These outbreaks revealed the lack of surveillance and preparedness of Madagascar and other ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Andriamandimby SF,Heraud JM,Randrianasolo L,Rafisandratantsoa JT,Andriamamonjy S,Richard V

    更新日期:2013-07-25 00:00:00

  • Spatio-temporal Genetic Structuring of Leishmania major in Tunisia by Microsatellite Analysis.

    abstract::In Tunisia, cases of zoonotic cutaneous leishmaniasis caused by Leishmania major are increasing and spreading from the south-west to new areas in the center. To improve the current knowledge on L. major evolution and population dynamics, we performed multi-locus microsatellite typing of human isolates from Tunisian go...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Harrabi M,Bettaieb J,Ghawar W,Toumi A,Zaâtour A,Yazidi R,Chaâbane S,Chalghaf B,Hide M,Bañuls AL,Ben Salah A

    更新日期:2015-08-24 00:00:00

  • Identification of microRNA genes in three opisthorchiids.

    abstract:BACKGROUND:Opisthorchis felineus, O. viverrini, and Clonorchis sinensis (family Opisthorchiidae) are parasitic flatworms that pose a serious threat to humans in some countries and cause opisthorchiasis/clonorchiasis. Chronic disease may lead to a risk of carcinogenesis in the biliary ducts. MicroRNAs (miRNAs) are small...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Ovchinnikov VY,Afonnikov DA,Vasiliev GV,Kashina EV,Sripa B,Mordvinov VA,Katokhin AV

    更新日期:2015-04-21 00:00:00

  • Impact of PGL-I seropositivity on the protective effect of BCG vaccination among leprosy contacts: a cohort study.

    abstract:BACKGROUND:Contacts of leprosy patients are at increased risk of developing leprosy and need to be targeted for early diagnosis. Seropositivity to the phenolic glycolipid I (PGL-I) antigen of Mycobacterium leprae has been used to identify contacts who have an increased risk of developing leprosy. In the present study, ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Düppre NC,Camacho LA,Sales AM,Illarramendi X,Nery JA,Sampaio EP,Sarno EN,Bührer-Sékula S

    更新日期:2012-01-01 00:00:00

  • Regulatory T cells in human lymphatic filariasis: stronger functional activity in microfilaremics.

    abstract::Infection with filarial parasites is associated with T cell hyporesponsiveness, which is thought to be partly mediated by their ability to induce regulatory T cells (Tregs) during human infections. This study investigates the functional capacity of Tregs from different groups of filarial patients to suppress filaria-s...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Wammes LJ,Hamid F,Wiria AE,Wibowo H,Sartono E,Maizels RM,Smits HH,Supali T,Yazdanbakhsh M

    更新日期:2012-01-01 00:00:00

  • Schistosoma mansoni infection suppresses the growth of Plasmodium yoelii parasites in the liver and reduces gametocyte infectivity to mosquitoes.

    abstract::Malaria and schistosomiasis are major parasitic diseases causing morbidity and mortality in the tropics. Epidemiological surveys have revealed coinfection rates of up to 30% among children in Sub-Saharan Africa. To investigate the impact of coinfection of these two parasites on disease epidemiology and pathology, we c...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Moriyasu T,Nakamura R,Deloer S,Senba M,Kubo M,Inoue M,Culleton R,Hamano S

    更新日期:2018-01-26 00:00:00

  • Advances in the diagnosis of endemic treponematoses: yaws, bejel, and pinta.

    abstract::Improved understanding of the differential diagnosis of endemic treponematoses is needed to inform clinical practice and to ensure the best outcome for a new global initiative for the eradication of yaws, bejel, and pinta. Traditionally, the human treponematoses have been differentiated based upon their clinical manif...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审


    authors: Mitjà O,Šmajs D,Bassat Q

    更新日期:2013-10-24 00:00:00

  • Declining antibody levels to Trypanosoma cruzi correlate with polymerase chain reaction positivity and electrocardiographic changes in a retrospective cohort of untreated Brazilian blood donors.

    abstract:BACKGROUND:Although infection with Trypanosoma cruzi is thought to be lifelong, less than half of those infected develop cardiomyopathy, suggesting greater parasite control or even clearance. Antibody levels appear to correlate with T. cruzi (antigen) load. We test the association between a downwards antibody trajector...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Buss LF,Campos de Oliveira-da Silva L,Moreira CHV,Manuli ER,Sales FC,Morales I,Di Germanio C,de Almeida-Neto C,Bakkour S,Constable P,Pinto-Filho MM,Ribeiro AL,Busch M,Sabino EC

    更新日期:2020-10-27 00:00:00

  • Rabies Risk: Difficulties Encountered during Management of Grouped Cases of Bat Bites in 2 Isolated Villages in French Guiana.

    abstract::In French Guiana, from 1984 to 2011, 14 animal rabies cases and 1 human rabies case (2008) were diagnosed. In January 2011, vampire-bat attacks occurred in 2 isolated villages. In mid-January, a medical team from the Cayenne Centre for Anti-Rabies Treatment visited the sites to manage individuals potentially exposed t...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Berger F,Desplanches N,Baillargeaux S,Joubert M,Miller M,Ribadeau-Dumas F,Spiegel A,Bourhy H

    更新日期:2013-06-27 00:00:00

  • Risk of Intestinal Parasitic Infections in People with Different Exposures to Wastewater and Fecal Sludge in Kampala, Uganda: A Cross-Sectional Study.

    abstract:BACKGROUND:There are health risks associated with wastewater and fecal sludge management and use, but little is known about the magnitude, particularly in rapidly growing urban settings of low- and middle-income countries. We assessed the point-prevalence and risk factors of intestinal parasite infections in people wit...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Fuhrimann S,Winkler MS,Kabatereine NB,Tukahebwa EM,Halage AA,Rutebemberwa E,Medlicott K,Schindler C,Utzinger J,Cissé G

    更新日期:2016-03-03 00:00:00

  • Chagas cardiomyopathy in the context of the chronic disease transition.

    abstract:BACKGROUND:Patients with Chagas disease have migrated to cities, where obesity, hypertension and other cardiac risk factors are common. METHODOLOGY/PRINCIPAL FINDINGS:The study included adult patients evaluated by the cardiology service in a public hospital in Santa Cruz, Bolivia. Data included risk factors for T. cru...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Hidron AI,Gilman RH,Justiniano J,Blackstock AJ,Lafuente C,Selum W,Calderon M,Verastegui M,Ferrufino L,Valencia E,Tornheim JA,O'Neal S,Comer R,Galdos-Cardenas G,Bern C,Chagas Disease Working Group in Peru and Bolivia.

    更新日期:2010-05-18 00:00:00

  • An agent-based model of tsetse fly response to seasonal climatic drivers: Assessing the impact on sleeping sickness transmission rates.

    abstract:BACKGROUND:This paper presents the development of an agent-based model (ABM) to incorporate climatic drivers which affect tsetse fly (G. m. morsitans) population dynamics, and ultimately disease transmission. The model was used to gain a greater understanding of how tsetse populations fluctuate seasonally, and investig...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Alderton S,Macleod ET,Anderson NE,Palmer G,Machila N,Simuunza M,Welburn SC,Atkinson PM

    更新日期:2018-02-09 00:00:00

  • Tissue invasion by Entamoeba histolytica: evidence of genetic selection and/or DNA reorganization events in organ tropism.

    abstract::Entamoeba histolytica infection may have various clinical manifestations. Nine out of ten E. histolytica infections remain asymptomatic, while the remainder become invasive and cause disease. The most common form of invasive infection is amebic diarrhea and colitis, whereas the most common extra-intestinal disease is ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Ali IK,Solaymani-Mohammadi S,Akhter J,Roy S,Gorrini C,Calderaro A,Parker SK,Haque R,Petri WA Jr,Clark CG

    更新日期:2008-04-09 00:00:00

  • The glutamine synthetase of Trypanosoma cruzi is required for its resistance to ammonium accumulation and evasion of the parasitophorous vacuole during host-cell infection.

    abstract::Trypanosoma cruzi, the etiological agent of Chagas disease, consumes glucose and amino acids depending on the environmental availability of each nutrient during its complex life cycle. For example, amino acids are the major energy and carbon sources in the intracellular stages of the T. cruzi parasite, but their consu...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Crispim M,Damasceno FS,Hernández A,Barisón MJ,Pretto Sauter I,Souza Pavani R,Santos Moura A,Pral EMF,Cortez M,Elias MC,Silber AM

    更新日期:2018-01-10 00:00:00

  • SARS-CoV-2 infection in China-Before the pandemic.

    abstract::In order to rapidly inform polices in the international response to the ongoing pandemic of coronavirus disease 19 (COVID-19), we summarize in this review current evidence on epidemiological and clinical features of the infection, transmission routes, problems of nucleic-acid testing, the epidemiological trend in Chin...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审


    authors: Liang H,Zheng L,Xia H,Tang J

    更新日期:2020-08-06 00:00:00

  • Heterogeneities in Leishmania infantum infection: using skin parasite burdens to identify highly infectious dogs.

    abstract:BACKGROUND:The relationships between heterogeneities in host infection and infectiousness (transmission to arthropod vectors) can provide important insights for disease management. Here, we quantify heterogeneities in Leishmania infantum parasite numbers in reservoir and non-reservoir host populations, and relate this ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Courtenay O,Carson C,Calvo-Bado L,Garcez LM,Quinnell RJ

    更新日期:2014-01-09 00:00:00

  • Distribution of Lutzomyia longipalpis chemotype populations in São Paulo state, Brazil.

    abstract:BACKGROUND:American visceral leishmaniasis (AVL) is an emerging disease in the state of São Paulo, Brazil. Its geographical expansion and the increase in the number of human cases has been linked to dispersion of Lutzomyia longipalpis into urban areas. To produce more accurate risk maps we investigated the geographic d...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Casanova C,Colla-Jacques FE,Hamilton JG,Brazil RP,Shaw JJ

    更新日期:2015-03-17 00:00:00

  • Anilinoquinoline based inhibitors of trypanosomatid proliferation.

    abstract::We recently reported the medicinal chemistry re-optimization of a series of compounds derived from the human tyrosine kinase inhibitor, lapatinib, for activity against Plasmodium falciparum. From this same library of compounds, we now report potent compounds against Trypanosoma brucei brucei (which causes human Africa...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Ferrins L,Sharma A,Thomas SM,Mehta N,Erath J,Tanghe S,Leed SE,Rodriguez A,Mensa-Wilmot K,Sciotti RJ,Gillingwater K,Pollastri MP

    更新日期:2018-11-26 00:00:00

  • Aedes aegypti D7 Saliva Protein Inhibits Dengue Virus Infection.

    abstract::Aedes aegypti is the primary vector of several medically relevant arboviruses including dengue virus (DENV) types 1-4. Ae. aegypti transmits DENV by inoculating virus-infected saliva into host skin during probing and feeding. Ae. aegypti saliva contains over one hundred unique proteins and these proteins have diverse ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Conway MJ,Londono-Renteria B,Troupin A,Watson AM,Klimstra WB,Fikrig E,Colpitts TM

    更新日期:2016-09-15 00:00:00

  • Bacterial community composition in the salivary glands of triatomines (Hemiptera: Reduviidae).

    abstract:BACKGROUND:Chagas disease is caused by the parasite Trypanosoma cruzi and is transmitted through triatomines (Hemiptera: Reduviidae). In the last year, many studies of triatomine gut microbiota have outlined its potential role in modulating vector competence. However, little is known about the microbiota present in the...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Lima MS,Laport MS,Lorosa ES,Jurberg J,Dos Santos KRN,da Silva Neto MAC,Rachid CTCDC,Atella GC

    更新日期:2018-09-13 00:00:00

  • Progress and impact of 13 years of the global programme to eliminate lymphatic filariasis on reducing the burden of filarial disease.

    abstract:BACKGROUND:A Global Programme to Eliminate Lymphatic Filariasis was launched in 2000, with mass drug administration (MDA) as the core strategy of the programme. After completing 13 years of operations through 2012 and with MDA in place in 55 of 73 endemic countries, the impact of the MDA programme on microfilaraemia, h...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Ramaiah KD,Ottesen EA

    更新日期:2014-11-20 00:00:00