Colonization factors among enterotoxigenic Escherichia coli isolates from children with moderate-to-severe diarrhea and from matched controls in the Global Enteric Multicenter Study (GEMS).


BACKGROUND:Enterotoxigenic Escherichia coli (ETEC) encoding heat-stable enterotoxin (ST) alone or with heat-labile enterotoxin (LT) cause moderate-to-severe diarrhea (MSD) in developing country children. The Global Enteric Multicenter Study (GEMS) identified ETEC encoding ST among the top four enteropathogens. Since the GEMS objective was to provide evidence to guide development and implementation of enteric vaccines and other interventions to diminish diarrheal disease morbidity and mortality, we examined colonization factor (CF) prevalence among ETEC isolates from children age <5 years with MSD and from matched controls in four African and three Asian sites. We also assessed strength of association of specific CFs with MSD. METHODOLOGY/PRINCIPAL FINDINGS:MSD cases enrolled at healthcare facilities over three years and matched controls were tested in a standardized manner for many enteropathogens. To identify ETEC, three E. coli colonies per child were tested by polymerase chain reaction (PCR) to detect genes encoding LT, ST; confirmed ETEC were examined by PCR for major CFs (Colonization Factor Antigen I [CFA/I] or Coli Surface [CS] antigens CS1-CS6) and minor CFs (CS7, CS12, CS13, CS14, CS17, CS18, CS19, CS20, CS21, CS30). ETEC from 806 cases had a single toxin/CF profile in three tested strains per child. Major CFs, components of multiple ETEC vaccine candidates, were detected in 66.0% of LT/ST and ST-only cases and were associated with MSD versus matched controls by conditional logistic regression (p≤0.006); major CFs detected in only 25.0% of LT-only cases weren't associated with MSD. ETEC encoding exclusively CS14, identified among 19.9% of 291 ST-only and 1.5% of 259 LT/ST strains, were associated with MSD (p = 0.0011). No other minor CF exhibited prevalence ≥5% and significant association with MSD. CONCLUSIONS/SIGNIFICANCE:Major CF-based efficacious ETEC vaccines could potentially prevent up to 66% of pediatric MSD cases due to ST-encoding ETEC in developing countries; adding CS14 extends coverage to ~77%.


PLoS Negl Trop Dis


Vidal RM,Muhsen K,Tennant SM,Svennerholm AM,Sow SO,Sur D,Zaidi AKM,Faruque ASG,Saha D,Adegbola R,Hossain MJ,Alonso PL,Breiman RF,Bassat Q,Tamboura B,Sanogo D,Onwuchekwa U,Manna B,Ramamurthy T,Kanungo S,Ahmed S,Q




Has Abstract


2019-01-04 00:00:00














  • New insights into leishmaniasis in the immunosuppressed.

    abstract::Immunosuppression contributes significantly to the caseload of visceral leishmaniasis (VL). HIV coinfection, solid organ transplantation, malnutrition, and helminth infections are the most important immunosuppression-related factors. This review briefly describes the challenges of these associations. East Africa and t...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审


    authors: Akuffo H,Costa C,van Griensven J,Burza S,Moreno J,Herrero M

    更新日期:2018-05-10 00:00:00

  • Leptospira Interrogans induces fibrosis in the mouse kidney through Inos-dependent, TLR- and NLR-independent signaling pathways.

    abstract:BACKGROUND:Leptospira (L.) interrogans are bacteria responsible for a worldwide reemerging zoonosis. Rodents carry L. interrogans asymptomatically in their kidneys and excrete bacteria in the urine, contaminating the environment. Humans get infected through skin contact and develop a mild or severe leptospirosis that m...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Fanton d'Andon M,Quellard N,Fernandez B,Ratet G,Lacroix-Lamandé S,Vandewalle A,Boneca IG,Goujon JM,Werts C

    更新日期:2014-01-30 00:00:00

  • Endogenous overexpression of an active phosphorylated form of DNA polymerase β under oxidative stress in Trypanosoma cruzi.

    abstract::Trypanosoma cruzi is exposed during its life to exogenous and endogenous oxidative stress, leading to damage of several macromolecules such as DNA. There are many DNA repair pathways in the nucleus and mitochondria (kinetoplast), where specific protein complexes detect and eliminate damage to DNA. One group of these p...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Rojas DA,Urbina F,Moreira-Ramos S,Castillo C,Kemmerling U,Lapier M,Maya JD,Solari A,Maldonado E

    更新日期:2018-02-12 00:00:00

  • Biodistribution and Toxicity Studies of PRINT Hydrogel Nanoparticles in Mosquito Larvae and Cells.

    abstract::Mosquito-borne diseases continue to remain major threats to human and animal health and impediments to socioeconomic development. Increasing mosquito resistance to chemical insecticides is a great public health concern, and new strategies/technologies are necessary to develop the next-generation of vector control tool...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Phanse Y,Dunphy BM,Perry JL,Airs PM,Paquette CC,Carlson JO,Xu J,Luft JC,DeSimone JM,Beaty BJ,Bartholomay LC

    更新日期:2015-05-21 00:00:00

  • Healthcare demand in response to rabies elimination campaigns in Latin America.

    abstract::The World Health Organization, the World Organization for Animal Health, and the Food and Agriculture Organization have resolved to eliminate human rabies deaths due to dog bites by 2030, and the Vaccine Alliance (Gavi) has added human rabies vaccines to their investments for 2021-2025. Implementing these goals cost-e...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Yoder J,Younce E,Lankester F,Palmer GH

    更新日期:2019-09-26 00:00:00

  • Zika virus like particles elicit protective antibodies in mice.

    abstract::Mosquito-borne Zika virus (ZIKV) typically causes a mild and self-limiting illness known as Zika fever. Since its recent emergence in 2014 in the American continent, ZIKV infection during pregnancy has been closely associated with a wide range of congenital abnormalities. To date, no vaccines or antivirals are publicl...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Salvo MA,Kingstad-Bakke B,Salas-Quinchucua C,Camacho E,Osorio JE

    更新日期:2018-02-05 00:00:00

  • Fulminant Amebic Colitis after Corticosteroid Therapy: A Systematic Review.

    abstract:BACKGROUND:Amebic colitis, caused by intestinal infection with the parasite, Entamoeba histolytica, is a common cause of diarrhea worldwide. Fulminant amebic colitis is the most devastating complication of this infection, associated with both high mortality and morbidity. We conducted a review of the English literature...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审


    authors: Shirley DA,Moonah S

    更新日期:2016-07-28 00:00:00

  • Outbreak of human brucellosis in Southern Brazil and historical review of data from 2009 to 2018.

    abstract:BACKGROUND:Human brucellosis (HB) is a bacterial zoonosis that is more frequent in low income and middle-income countries; it is sometimes associated with outbreaks. The aim of this study was to describe the largest outbreak of HB in Brazil. METHODS:A retrospective cohort study of patients suspected of having contract...

    journal_title:PLoS neglected tropical diseases

    pub_type: 历史文章,杂志文章


    authors: Lemos TS,Cequinel JC,Costa TP,Navarro AB,Sprada A,Shibata FK,Gondolfo R,Tuon FF

    更新日期:2018-09-18 00:00:00

  • Antibodies against immature virions are not a discriminating factor for dengue disease severity.

    abstract::Humoral immunity plays an important role in controlling dengue virus (DENV) infection. Antibodies (Abs) developed during primary infection protect against subsequent infection with the same dengue serotype, but can enhance disease following secondary infection with a heterologous serotype. A DENV virion has two surfac...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Rodenhuis-Zybert IA,da Silva Voorham JM,Torres S,van de Pol D,Smit JM

    更新日期:2015-03-11 00:00:00

  • Major risk factors and histopathological profile of treatment failure, relapse and chronic patients with anthroponotic cutaneous leishmaniasis: A prospective case-control study on treatment outcome and their medical importance.

    abstract::Over the last years, there has been a remarkable increase in the number of unresponsive patients with anthroponotic cutaneous leishmaniasis (ACL) reported worldwide. The primary objective of this study was to explore the role of demographic, clinical and environmental risk related-factors in the development of treatme...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Bamorovat M,Sharifi I,Dabiri S,Shamsi Meymandi S,Karamoozian A,Amiri R,Heshmatkhah A,Borhani Zarandi M,Aflatoonian MR,Sharifi F,Kheirandish R,Hassanzadeh S

    更新日期:2021-01-28 00:00:00

  • Diagnostic accuracy of Kato-Katz and FLOTAC for assessing anthelmintic drug efficacy.

    abstract:BACKGROUND:Sensitive diagnostic tools are required for an accurate assessment of prevalence and intensity of helminth infections in areas undergoing regular deworming, and for monitoring anthelmintic drug efficacy. We compared the diagnostic accuracy of the Kato-Katz and FLOTAC techniques in the frame of a drug efficac...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Knopp S,Speich B,Hattendorf J,Rinaldi L,Mohammed KA,Khamis IS,Mohammed AS,Albonico M,Rollinson D,Marti H,Cringoli G,Utzinger J

    更新日期:2011-04-12 00:00:00

  • Stratified sero-prevalence revealed overall high disease burden of dengue but suboptimal immunity in younger age groups in Pune, India.

    abstract:BACKGROUND:In India, dengue disease is emerging as the most important vector borne public health problem due to rapid and unplanned urbanization, high human density and week management of the disease. Clinical cases are grossly underreported and not much information is available on prevalence and incidence of the disea...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Mishra AC,Arankalle VA,Gadhave SA,Mahadik PH,Shrivastava S,Bhutkar M,Vaidya VM

    更新日期:2018-08-06 00:00:00

  • Rift valley Fever virus encephalitis is associated with an ineffective systemic immune response and activated T cell infiltration into the CNS in an immunocompetent mouse model.

    abstract:BACKGROUND:Rift Valley fever virus (RVFV) causes outbreaks of severe disease in livestock and humans throughout Africa and the Arabian Peninsula. In people, RVFV generally causes a self-limiting febrile illness but in a subset of individuals, it progresses to more serious disease. One manifestation is a delayed-onset e...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Dodd KA,McElroy AK,Jones TL,Zaki SR,Nichol ST,Spiropoulou CF

    更新日期:2014-06-12 00:00:00

  • Potential animal reservoirs (dogs and bats) of human visceral leishmaniasis due to Leishmania infantum in French Guiana.

    abstract::In French Guiana, cutaneous leishmaniasis is highly endemic, whereas no autochthonous case of visceral leishmaniasis have been reported so far. However, due to its proximity to Brazil which is highly endemic for visceral leishmaniasis, and the high transboundary population flow, an epidemiological challenge could aris...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Medkour H,Davoust B,Dulieu F,Maurizi L,Lamour T,Marié JL,Mediannikov O

    更新日期:2019-06-19 00:00:00

  • Epidemiology of Infant Dengue Cases Illuminates Serotype-Specificity in the Interaction between Immunity and Disease, and Changes in Transmission Dynamics.

    abstract:BACKGROUND:Infants born to dengue immune mothers acquire maternal antibodies to dengue. These antibodies, though initially protective, decline during the first year of life to levels thought to be disease enhancing, before reaching undetectable levels. Infants have long been studied to understand the interaction betwee...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Clapham H,Cummings DA,Nisalak A,Kalayanarooj S,Thaisomboonsuk B,Klungthong C,Fernandez S,Srikiatkhachorn A,Macareo LR,Lessler J,Reiser J,Yoon IK

    更新日期:2015-12-11 00:00:00

  • Turning poop into profit: Cost-effectiveness and soil transmitted helminth infection risk associated with human excreta reuse in Vietnam.

    abstract::Human excreta is a low cost source of nutrients vital to plant growth, but also a source of pathogens transmissible to people and animals. We investigated the cost-savings and infection risk of soil transmitted helminths (STHs) in four scenarios where farmers used either inorganic fertilizer or fresh/composted human e...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,meta分析,评审


    authors: Tran-Thi N,Lowe RJ,Schurer JM,Vu-Van T,MacDonald LE,Pham-Duc P

    更新日期:2017-11-27 00:00:00

  • Clinical, etiological and epidemiological investigations of hand, foot and mouth disease in southern Vietnam during 2015 - 2018.

    abstract::Hand, foot and mouth disease (HFMD) continues to challenge Asia with pandemic potential. In Vietnam, there have been two major outbreaks occurring during 2011-2012 (>200,000 hospitalizations and >200 deaths) and more recently in 2018 (>130,000 hospitalizations and 17 deaths). Given the high burden and the complex epid...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Nhan LNT,Khanh TH,Hong NTT,Van HMT,Nhu LNT,Ny NTH,Nguyet LA,Thanh TT,Anh NT,Hang VTT,Qui PT,Viet HL,Tung TH,Ha DQ,Tuan HM,Thwaites G,Chau NVV,Thwaites L,Hung NT,van Doorn HR,Tan LV

    更新日期:2020-08-17 00:00:00

  • The detection of anti-dengue virus IgM in urine in participants enrolled in an acute febrile illness study in Puerto Rico.

    abstract:BACKGROUND:Dengue is an important arboviral disease with about 100 million dengue cases per year, of which, ~5% result in severe disease. Clinical differentiation of dengue from other acute febrile illnesses (AFI) is difficult, and diagnostic blood tests are costly. We evaluated the utility of anti-DENV IgM in urine to...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Caraballo E,Poole-Smith BK,Tomashek KM,Torres-Velasquez B,Alvarado LI,Lorenzi OD,Ramos C,Carrión J,Hunsperger E

    更新日期:2020-01-29 00:00:00

  • Contrasting spatial distribution and risk factors for past infection with scrub typhus and murine typhus in Vientiane City, Lao PDR.

    abstract:BACKGROUND:The aetiological diagnostic of fevers in Laos remains difficult due to limited laboratory diagnostic facilities. However, it has recently become apparent that both scrub and murine typhus are common causes of previous undiagnosed fever. Epidemiological data suggests that scrub typhus would be more common in ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Vallée J,Thaojaikong T,Moore CE,Phetsouvanh R,Richards AL,Souris M,Fournet F,Salem G,Gonzalez JP,Newton PN

    更新日期:2010-12-07 00:00:00

  • Wolbachia enhances West Nile virus (WNV) infection in the mosquito Culex tarsalis.

    abstract::Novel strategies are required to control mosquitoes and the pathogens they transmit. One attractive approach involves maternally inherited endosymbiotic Wolbachia bacteria. After artificial infection with Wolbachia, many mosquitoes become refractory to infection and transmission of diverse pathogens. We evaluated the ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Dodson BL,Hughes GL,Paul O,Matacchiero AC,Kramer LD,Rasgon JL

    更新日期:2014-07-10 00:00:00

  • Prevalence and risk factors for echinococcal infection in a rural area of northern Chile: a household-based cross-sectional study.

    abstract:BACKGROUND:Hydatidosis is a zoonotic disease of worldwide distribution caused by Echinococcus granulosus. Our study aimed to determine the prevalence of human and canine echinococcosis as well as the associated risk factors in a rural area of the Limarí province in northern Chile. METHODOLOGY/PRINCIPAL FINDINGS:A cros...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Acosta-Jamett G,Weitzel T,Boufana B,Adones C,Bahamonde A,Abarca K,Craig PS,Reiter-Owona I

    更新日期:2014-08-28 00:00:00

  • The Effect of Deworming on Growth in One-Year-Old Children Living in a Soil-Transmitted Helminth-Endemic Area of Peru: A Randomized Controlled Trial.

    abstract:BACKGROUND:Appropriate health and nutrition interventions to prevent long-term adverse effects in children are necessary before two years of age. One such intervention may include population-based deworming, recommended as of 12 months of age by the World Health Organization in soil-transmitted helminth (STH)-endemic a...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,随机对照试验


    authors: Joseph SA,Casapía M,Montresor A,Rahme E,Ward BJ,Marquis GS,Pezo L,Blouin B,Maheu-Giroux M,Gyorkos TW

    更新日期:2015-10-01 00:00:00

  • Polyphenol-Rich Diets Exacerbate AMPK-Mediated Autophagy, Decreasing Proliferation of Mosquito Midgut Microbiota, and Extending Vector Lifespan.

    abstract:BACKGROUND:Mosquitoes feed on plant-derived fluids such as nectar and sap and are exposed to bioactive molecules found in this dietary source. However, the role of such molecules on mosquito vectorial capacity is unknown. Weather has been recognized as a major determinant of the spread of dengue, and plants under abiot...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Nunes RD,Ventura-Martins G,Moretti DM,Medeiros-Castro P,Rocha-Santos C,Daumas-Filho CR,Bittencourt-Cunha PR,Martins-Cardoso K,Cudischevitch CO,Menna-Barreto RF,Oliveira JH,Gusmão DS,Alves Lemos FJ,Alviano DS,Oliveira PL,Low

    更新日期:2016-10-12 00:00:00

  • Both living bacteria and eukaryotes in the mosquito gut promote growth of larvae.

    abstract::We recently reported that larval stage Aedes aegypti and several other species of mosquitoes grow when living bacteria are present in the gut but do not grow when living bacteria are absent. We further reported that living bacteria induce a hypoxia signal in the gut, which activates hypoxia-induced transcription facto...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Valzania L,Martinson VG,Harrison RE,Boyd BM,Coon KL,Brown MR,Strand MR

    更新日期:2018-07-06 00:00:00

  • Social pathways for Ebola virus disease in rural Sierra Leone, and some implications for containment.

    abstract::The current outbreak of Ebola Virus Disease in Upper West Africa is the largest ever recorded. Molecular evidence suggests spread has been almost exclusively through human-to-human contact. Social factors are thus clearly important to understand the epidemic and ways in which it might be stopped, but these factors hav...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Richards P,Amara J,Ferme MC,Kamara P,Mokuwa E,Sheriff AI,Suluku R,Voors M

    更新日期:2015-04-17 00:00:00

  • Comparative genomic and phenotypic characterization of invasive non-typhoidal Salmonella isolates from Siaya, Kenya.

    abstract::Non-typhoidal Salmonella (NTS) is a major global health concern that often causes bloodstream infections in areas of the world affected by malnutrition and comorbidities such as HIV and malaria. Developing a strategy to control the emergence and spread of highly invasive and antimicrobial resistant NTS isolates requir...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Kubicek-Sutherland JZ,Xie G,Shakya M,Dighe PK,Jacobs LL,Daligault H,Davenport K,Stromberg LR,Stromberg ZR,Cheng Q,Kempaiah P,Ong'echa JM,Otieno V,Raballah E,Anyona S,Ouma C,Chain PSG,Perkins DJ,Mukundan H,McMahon BH

    更新日期:2021-02-01 00:00:00

  • Geospatial distribution of intestinal parasitic infections in Rio de Janeiro (Brazil) and its association with social determinants.

    abstract:BACKGROUND:Intestinal parasitic infections remain among the most common infectious diseases worldwide. This study aimed to estimate their prevalence and provide a detailed analysis of geographical distribution of intestinal parasites in the metropolitan region of Rio de Janeiro, considering demographic, socio-economic,...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Faria CP,Zanini GM,Dias GS,da Silva S,de Freitas MB,Almendra R,Santana P,Sousa MD

    更新日期:2017-03-08 00:00:00

  • Estimating the cost of illness and burden of disease associated with the 2014-2015 chikungunya outbreak in the U.S. Virgin Islands.

    abstract::Chikungunya virus (CHIKV), an alphavirus that causes fever and severe polyarthralgia, swept through the Americas in 2014 with almost 2 million suspected or confirmed cases reported by April 2016. In this study, we estimate the direct medical costs, cost of lost wages due to absenteeism, and years lived with disability...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Feldstein LR,Ellis EM,Rowhani-Rahbar A,Hennessey MJ,Staples JE,Halloran ME,Weaver MR

    更新日期:2019-07-19 00:00:00

  • Double impact: natural molluscicide for schistosomiasis vector control also impedes development of Schistosoma mansoni cercariae into adult parasites.

    abstract:BACKGROUND:Schistosomiasis has been reported in 78 endemic countries and affects 240 million people worldwide. The digenetic parasite Schistosoma mansoni needs fresh water to compete its life cycle. There, it is susceptible to soluble compounds that can affect directly and/or indirectly the parasite's biology. The cerc...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Augusto RC,Tetreau G,Chan P,Walet-Balieu ML,Mello-Silva CC,Santos CP,Grunau C

    更新日期:2017-07-28 00:00:00

  • Perceptions about interventions to control schistosomiasis among the Lake Victoria island communities of Koome, Uganda.

    abstract:BACKGROUND:Praziquantel-based mass treatment is the main approach to controlling schistosomiasis mansoni in endemic areas. Interventions such as provision and use of safe water, minimising contact with infested water, disposal of stool in latrines and snail control provide key avenues to break the transmission cycle an...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Sanya RE,Tumwesige E,Elliott AM,Seeley J

    更新日期:2017-10-02 00:00:00