Virus detection and identification using random multiplex (RT)-PCR with 3'-locked random primers.

abstract：BACKGROUND:Otitis media (OM) affects ≥80% of children before the age of three. OM can arise following co-infection with influenza A virus (IAV) and the bacterium Streptococcus pneumoniae. We have previously shown that H3 IAV strains (such as Udorn/72) induced a higher rate of bacterial OM than H1 strains (such as PR8/3...

journal_title：Virology journal

authors： Short KR,Habets MN,Payne J,Reading PC,Diavatopoulos DA,Wijburg OL

更新日期：2013-04-23 00:00:00

abstract：BACKGROUND:Lymphocytes provide invaluable whistle blowers of changes due to infections. We use the information registered by these cells using their mRNAs as they encounter the pathogen to develop patterns of expression that correspond to that specific pathogen. Venezuelan equine encephalitis (VEE) is a mosquito-borne ...

journal_title：Virology journal

authors： Hammamieh R,Barmada M,Ludwig G,Peel S,Koterski N,Jett M

更新日期：2007-08-29 00:00:00

abstract：:Viral diseases constitute obstacles to pepper production in the world. In Nigeria, pepper plants are primarily affected by pepper veinal mottle virus (PVMV), Cucumber mosaic virus (CMV), Pepper leaf curl Virus (TLCV), Tobacco mosaic virus (TMV), Pepper mottle virus (PMV) and a host of other viruses. The experiment was...

journal_title：Virology journal

authors： Arogundade O,Balogun OS,Kareem KT

更新日期：2012-04-11 00:00:00

abstract：BACKGROUND:Oral malignancy is a major global health problem. Besides the main risk factors of tobacco, smoking and alcohol, infection by human papillomavirus (HPV) and genetic alterations are likely to play an important role in these lesions. The purpose of this study was to compare the efficacy of HC-II assay and PCR ...

journal_title：Virology journal

authors： Chaudhary AK,Pandya S,Mehrotra R,Bharti AC,Singh M,Singh M

更新日期：2010-09-23 00:00:00

abstract：:A TaqMan-based real-time polymerase chain reaction (PCR) assay was devised for the detection of porcine parvovirus (PPV). Two primers and a TaqMan probe for the non-structural protein NS1 gene were designed. The detection limit was 1 x 10² DNA copies/μL, and the assay was linear in the range of 1 x 10² to 1 x 10⁹ copi...

journal_title：Virology journal

authors： Song C,Zhu C,Zhang C,Cui S

更新日期：2010-12-02 00:00:00

abstract：BACKGROUND:Hepatitis C virus (HCV) infection is a major public health problem with more than 170 million cases of chronic infections worldwide. There is no protective vaccine currently available for HCV, therefore the development of novel strategy to prevent chronic infection is important. We reported earlier that a re...

journal_title：Virology journal

authors： Chandra PK,Hazari S,Poat B,Gunduz F,Prabhu R,Liu G,Burioni R,Clementi M,Garry RF,Dash S

更新日期：2010-06-07 00:00:00

abstract：BACKGROUND:HIV infection of cells varies greatly between individuals, with multiple steps in the replication cycle potentially contributing to the variability. Although entry and post-entry variability of HIV infection levels in cells has been demonstrated, variability in HIV binding has not been examined. In this stud...

journal_title：Virology journal

authors： Anzinger JJ,Olinger GG,Spear GT

更新日期：2008-08-15 00:00:00

abstract：BACKGROUND:Rodent borne hantaviruses are emerging viruses infecting humans through inhalation. They cause hemorrhagic fever with renal syndrome and hemorrhagic cardiopulmonary syndrome. Recently, hantaviruses have been detected in other small mammals such as Soricomorpha (shrews, moles) and Chiroptera (bats), suggested...

journal_title：Virology journal

authors： Lwande OW,Mohamed N,Bucht G,Ahlm C,Olsson G,Evander M

更新日期：2020-12-29 00:00:00

abstract：:The triple-gene-block protein 2 (TGBp2) of Bamboo mosaic virus (BaMV) is a transmembrane protein which was proposed to be involved in viral RNA binding during virus transport. Here, we report on the RNA-binding properties of TGBp2. Using tyrosine fluorescence spectroscopy and UV-crosslinking assays, the TGBp2 solubili...

journal_title：Virology journal

authors： Hsu HT,Tseng YH,Chou YL,Su SH,Hsu YH,Chang BY

更新日期：2009-05-07 00:00:00

abstract：BACKGROUND:The diseases caused by Rice black streaked dwarf virus (RBSDV) and Southern rice black streaked dwarf virus (SRBSDV) have been occurring epidemically in China and southeastern Asia in recent years. A sensitive, reliable and quantitative method is required to detect and distinguish for RBSDV and SRBSDV in ric...

journal_title：Virology journal

authors： Zhang P,Mar TT,Liu W,Li L,Wang X

更新日期：2013-01-18 00:00:00

abstract：:Hepatitis E virus (HEV) is a zoonotic pathogen of which several species of animal were reported as reservoirs. Swine stands out as the major reservoir for HEV infection in humans, as suggested by the close genetic relationship of swine and human virus and cross-species infection of HEV. Up to now, the mechanism of cro...

journal_title：Virology journal

authors： Zhang W,Hua X,Shen Q,Yang S,Yin H,Cui L

更新日期：2011-10-27 00:00:00

abstract：:Lytic bacteriophage 0305phi8-36 forms visually observed aggregates during plaque formation. Aggregates intrinsically lower propagation potential. In the present study, the following observations indicate that lost propagation potential is regained with time: (1) Aggregates sometimes concentrate at the edge of clear pl...

journal_title：Virology journal

authors： Serwer P,Hayes SJ,Lieman K

更新日期：2007-12-04 00:00:00

abstract：BACKGROUND:The high genetic diversity of HIV-1 has been shown to influence the global distribution, disease progression, treatment success, and the development of an effective vaccine. Despite the low HIV prevalence in Cameroon, all the major HIV subtypes alongside several circulating recombinant forms (CRFs) and uniqu...

journal_title：Virology journal

authors： Abongwa LE,Nyamache AK,Torimiro JN,Okemo P,Charles F

更新日期：2019-08-15 00:00:00

abstract：BACKGROUND:Torque Teno Virus (TTV) was the first single stranded circular DNA virus to be discovered that infects humans. Although there have been numerous reports regarding the prevalence of TTV from other countries of South Asia, there is severe lack of information regarding its prevalence in Pakistan. Thus the prese...

journal_title：Virology journal

authors： Hussain T,Manzoor S,Waheed Y,Tariq H,Hanif K

更新日期：2012-12-28 00:00:00

abstract：BACKGROUND:Tumor necrosis factor-alpha-induced protein 8-like 2 (TIPE2) is a novel target and molecule in the negative regulation of immune homeostasis. The present study aimed to investigate the dynamic expression of TIPE2 mRNA during the progression of chronic hepatitis B virus (HBV) infection. METHODS:A total of 19...

journal_title：Virology journal

authors： Liu Y,Jin J,Ji J,Gao XM,Fan YC

更新日期：2019-10-28 00:00:00

abstract：BACKGROUND:Xenotransplantation using pig cells, tissues and organs may help to overcome the shortage of human tissues and organs for the treatment of tissue and organ failure. Progress in the prevention of immunological rejection using genetically modified pigs and new, more effective, immunosuppression regimens will a...

journal_title：Virology journal

authors： Denner J

更新日期：2018-11-08 00:00:00

abstract：BACKGROUND:Influenza viruses are characterized by their highly variable surface proteins HA and NA. The third surface protein M2 is a nearly invariant protein in all Influenza A strains. Despite extensive studies in other animal models, this study is the first to describe the use of recombinant M2 protein and a peptide...

journal_title：Virology journal

authors： Swinkels WJ,Hoeboer J,Sikkema R,Vervelde L,Koets AD

更新日期：2013-06-24 00:00:00

abstract：BACKGROUND:The deciphering of cellular networks to determine susceptibility to infection by HIV or the related simian immunodeficiency virus (SIV) is a major challenge in infection biology. RESULTS:Here, we have compared gene expression profiles of a human CD4+ T cell line at 24 h after infection with a cell line of t...

journal_title：Virology journal

authors： He FQ,Sauermann U,Beer C,Winkelmann S,Yu Z,Sopper S,Zeng AP,Wirth M

更新日期：2014-08-27 00:00:00

abstract：BACKGROUND:Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by SFTS bunyavirus (SFTSV), a tick borne bunyavirus. However, Immunohistochemistry of SFTS patients are not well studied. METHODS:We obtained multiple of tissues from a fatal case with SFTS, including blood, lungs, k...

journal_title：Virology journal

authors： Li S,Li Y,Wang Q,Yu X,Liu M,Xie H,Qian L,Ye L,Yang Z,Zhang J,Zhu H,Zhang W

更新日期：2018-05-30 00:00:00

abstract：BACKGROUND:Myxoma virus is a member of the Poxviridae and causes disease in European rabbits. Laboratory confirmation of the clinical disease, which occurs in the autumn of most years in Denmark, has been achieved previously using antigen ELISA and electron microscopy. RESULTS:An unusually large number of clinically s...

journal_title：Virology journal

authors： Belsham GJ,Polacek C,Breum SØ,Larsen LE,Bøtner A

更新日期：2010-01-16 00:00:00

abstract：BACKGROUND:The BK Polyomavirus (BKPyV) and JC polyomavirus (JCPyV) infections are widespread in human population and have been associated with severe kidney and brain disorders, respectively. The viruses remain latent primarily in reno-urinary tract, reactivating only in case of a compromised immune system. The seroepi...

journal_title：Virology journal

authors： Hussain I,Tasneem F,Umer M,Pervaiz A,Raza M,Arshad MI,Shahzad N

更新日期：2017-04-24 00:00:00

abstract：BACKGROUND:Due to potential advantages, human adenoviral vectors have been evaluated pre-clinically as recombinant vaccine vectors against several cancers and infectious diseases, including human immunodeficiency virus (HIV) infection. The V3 loop of HIV-1 glycoprotein 120 (gp120) contains important neutralizing epitop...

journal_title：Virology journal

authors： Gu L,Krendelchtchikova V,Krendelchtchikov A,Oster RA,Fujihashi K,Matthews QL

更新日期：2014-06-16 00:00:00

abstract：: Previously, we explored the epidemic pattern and molecular characterization of enteroviruses isolated in Chungnam, Korea from 2005 to 2006. The present study extended these observations to 2008 and 2009. In this study, enteroviruses showed similar seasonal prevalent pattern from summer to fall and age distribution to...

journal_title：Virology journal

authors： Baek K,Yeo S,Lee B,Park K,Song J,Yu J,Rheem I,Kim J,Hwang S,Choi Y,Cheon D,Park J

更新日期：2011-06-13 00:00:00

abstract：BACKGROUND:Highly pathogenic avian influenza virus (AIV) subtype H5N1 remains a threat to poultry. Duck enteritis virus (DEV)-vectored vaccines expressing AIV H5N1 hemagglutinin (HA) may be viable AIV and DEV vaccine candidates. METHODS:To facilitate the generation and further improvement of DEV-vectored HA(H5) vaccin...

journal_title：Virology journal

authors： Wang J,Ge A,Xu M,Wang Z,Qiao Y,Gu Y,Liu C,Liu Y,Hou J

更新日期：2015-08-13 00:00:00

abstract：:RNA recombination is one of the two major factors that create RNA genome variability. Assessing its incidence in plant RNA viruses helps understand the formation of new isolates and evaluate the effectiveness of crop protection strategies. To search for recombination in Soybean mosaic virus (SMV), the causal agent of ...

journal_title：Virology journal

authors： Gagarinova AG,Babu M,Strömvik MV,Wang A

更新日期：2008-11-26 00:00:00

abstract：BACKGROUND:Sheeppox (SPP) and goatpox (GTP) caused by sheeppox virus (SPPV) and goatpox virus (GTPV), respectively of the genus Capripoxvirus in the family Poxviridae, are severely afflicting small ruminants' production systems in Africa and Asia. In endemic areas, SPP and GTP are controlled using vaccination with live...

journal_title：Virology journal

authors： Chibssa TR,Grabherr R,Loitsch A,Settypalli TBK,Tuppurainen E,Nwankpa N,Tounkara K,Madani H,Omani A,Diop M,Cattoli G,Diallo A,Lamien CE

更新日期：2018-04-02 00:00:00

abstract：:Marek's disease is a highly contagious disease of poultry characterized by rapid-on set of T-cell lymphomas, which is caused by Marek's disease virus (MDV), but its pathogenic mechanism is still not very clear. Recently, some new progress were achieved in molecular character of MDV. Along with the genomic sequencing o...

journal_title：Virology journal

authors： Tian M,Zhao Y,Shi M,Lin Y,Zou N,Liu P,Wen X,Cao S,Huang Y

更新日期：2010-12-23 00:00:00

abstract：BACKGROUND:Hepatitis A virus is an infection of liver; it is hyperendemic in vast areas of the world including India. In most cases it causes an acute self limited illness but rarely fulminant. There is growing concern about change in pattern from asymptomatic childhood infection to an increased incidence of symptomati...

journal_title：Virology journal

authors： Hussain Z,Husain SA,Almajhdi FN,Kar P

更新日期：2011-05-23 00:00:00

abstract：BACKGROUND:Swine-origin virus infection spreading widely could cause significant economic loss to porcine industry. Novel antiviral agents need to be developed to control this situation. METHODS:In this study, we evaluated the activities of five broad-spectrum antimicrobial peptides (AMPs) against several important sw...

journal_title：Virology journal

authors： Hu H,Guo N,Chen S,Guo X,Liu X,Ye S,Chai Q,Wang Y,Liu B,He Q

更新日期：2019-07-31 00:00:00

abstract：BACKGROUND:Human caliciviruses (HuCV) are emerging enteric pathogens that are a common cause of diarrhea in humans worldwide. Due to the paucity of information on the molecular characterization of HuCV circulating in Mexico, the aim of this work was to investigate the diversity and molecular epidemiology of the HuCV in...

journal_title：Virology journal

authors： Gómez-Santiago F,Ribas-Aparicio RM,García-Lozano H

更新日期：2012-02-23 00:00:00