Abstract:
:The mechanisms underlying the antifertility effects of hyperprolactinemia have yet to be established in an appropriate experimental model. Hyperprolactinemia is a known side effect of fluphenazine, a broad spectrum, long-acting phenothiazine known to be dopamine type-D2 receptor antagonist. In our earlier study in adult male rats, we reported that fluphenazine at a dose of 3 mg/kg/day suppressed serum FSH but not testosterone (T) through increasing dopamine (DA) metabolism in the pituitary gland, within 60 days. Fluphenazine treatment affected sperm quality and male rats treated with fluphenazine sired fewer litters. The effects of fluphenazine-induced hyperprolactinemia on sperm quality appeared to be related to reduced FSH. We now report that FSH suppression enhanced the uptake of acridine orange (AO), a DNA intercalating, fluorescent dye by the fluphenazine-treated caput epididymal sperms with concomitant reduction in the uptake of thiol-specific monobromobimane (mBBr) fluorescent dye in vitro, suggesting greater accessibility of DNA intercalating dye to sperm chromatin and reduction in free sperm protein thiols. The concomitant increase in AO and decrease in mBBr fluorescence was suggestive of loose chromatin packaging in caput epididymal sperms after treatment with fluphenazine at 3 mg/kg/day for 60 days. The suppression in levels of protamine (P1) in caput epididymal sperms suggested that chromatin hypocompaction was due to reduced deposition of protamines in sperm chromatin. Reduction in testicular levels of cyclic adenosyl 3', 5' monophosphate response element modulator (CREMtau) and P1 further suggested that reduced deposition was indeed due to reduced synthesis. The concomitant reduction in testicular levels of transition protein 1 (TP1) and transition protein 2 (TP2) also suggested that hypoprotamination was due to reduced synthesis of these proteins crucial for facilitating P1 deposition. The effect appeared to have occurred at the level of translation of CREMtau, since its transcript levels were unaffected whereas those of TP1, TP2 and P1 and protamine were upregulated. The study led to the view that the effects of FSH suppression were manifest on the posttranscriptional modifications of CREMtau, as also on transcript repression of TP1, TP2, P1, which do the RNA- binding proteins bring about. Reduction in FSH did not decrease ABP expression in the testis, which has recently been implicated in the expression of transition protein 1 in vitro. However, a significant reduction was evident after fluphenazine treatment, in the immunoexpression of testicular cAMP, the mediator of FSH effects in the Sertoli cells and putative mediator of ABP effects in the spermatids. The study suggests that fluphenazine-induced hyperprolactinemia suppressed FSH and affected a putative cAMP-dependent mechanism underlying posttranscriptional modification of spermatidal genes involved in chromatin condensation, presumably by reducing the availability/secretion of ABP, a paracrine regulator of spermiogenesis in vitro.
journal_name
J Endocrinol Investjournal_title
Journal of endocrinological investigationauthors
Aleem M,Choudhari J,Padwal V,Balasinor N,Parte P,Gill-Sharma MKdoi
10.1007/BF03345528keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
39-48issue
1eissn
0391-4097issn
1720-8386pii
2905journal_volume
28pub_type
杂志文章abstract::The syndrome of apparent mineralocorticoid syndrome (AME) results from defective 11beta-hydroxysteroid dehydrogenase 2 (11beta-HSD2). This enzyme is co-expressed with the mineralocorticoid receptor (MR) in the kidney and converts cortisol to its inactive metabolite cortisone. Its deficiency allows the unmetabolized co...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03343755
更新日期:2000-07-01 00:00:00
abstract::Anatomy studies normally precede physiology. While the anatomy of the penis and the biochemical and molecular regulation of erection are largely known, the exact anatomical description of the human clitoris was produced in 1998, the taxonomy of female sexual dysfunctions classified in 1999, and biochemistry of female ...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Cimetidine, an H2 receptor antagonist, is a potent stimulant of PRL secretion in normal women. In the present study, the PRL response to cimetidine was studied in 34 normal pregnant women and their fetuses during labor. Serum maternal PRL levels increased significantly after the acute iv injection of 400 mg cimetidine...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03350225
更新日期:1988-12-01 00:00:00
abstract:OBJECTIVE:To investigate the prevalence of cardiovascular risk factors in patients with primary hyperparathyroidism (PHPT) and to determine if they are gender specific. METHODS:This was a retrospective case control study. Three hundred and sixty-three men and women with PHPT and 363 age-, sex-, and body mass index (BM...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.3275/7861
更新日期:2012-06-01 00:00:00
abstract:OBJECTIVE:Blepharophimosis syndrome (BPES) is an autosomal dominant genetic condition resulting from heterozygous mutations in the FOXL2 gene and clinically characterized by an eyelid malformation associated (type I) or not (type II) with premature ovarian failure. The distinction between the two forms is critical for ...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-015-0334-3
更新日期:2016-02-01 00:00:00
abstract:PURPOSE:The review focused on the role that media reporting plays in the level of public awareness about osteoporosis and its influence on osteoporosis treatment decisions. METHODS:We reviewed the literature on the role of media on three main aspects influencing patient adherence to osteoporosis treatment: the awarene...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:10.1007/s40618-018-0898-9
更新日期:2018-12-01 00:00:00
abstract::The distribution of goiter prevalence in schoolchildren (no.=13,984, age 6-14 yr), the neonatal TSH results obtained from the congenital hypothyroidism screening program and the urinary iodine excretion values (no.=284) were employed for the assessment of iodine deficiency in Calabria, a Southern Italy region. Data we...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03343991
更新日期:2002-03-01 00:00:00
abstract::Metabolic diseases are associated with chronic low-grade inflammation, which has been indicated as a potential mediator of endothelial dysfunction and cardiovascular disease. Visceral adiposity is thought to be the starting condition of the inflammatory state through the release of inflammatory cytokines, including TN...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:10.1007/s40618-018-0872-6
更新日期:2018-11-01 00:00:00
abstract::Aromatase activity for androstenedione was measured in stromal cells of human uterine endometrium throughout the menstrual cycle. The effect of progestogen on aromatase activity was also examined in the endometrial stromal cells. Aromatase activity tended to be high in the proliferative cells compared to the secretory...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03349962
更新日期:1989-03-01 00:00:00
abstract:AIM:The optimal GH regimen, in terms of cost-effectiveness, in children with normal GH immunoreactivity but reduced bioactivity is still debated. METHODS:In 12 GH-deficient (GHD) and 12 bioinactive GH children undergoing GH treatment we evaluated the increase in growth velocity, the difference between target height an...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03347461
更新日期:2011-05-01 00:00:00
abstract::In healthy pregnant women, the regulation of thyroid function depends upon several factors. Three factors act independently to increase thyroid hormone requirements: 1) the marked increase in the binding capacity of serum due to high TBG levels; 2) the direct stimulation of the thyroid by human chorionic gonadotropin,...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:10.1007/BF03348861
更新日期:1993-05-01 00:00:00
abstract::The status of the erythrocyte insulin receptor was investigated prior to and during storage at 4C in acid-citrate dextrose (ACD) solution. The receptors on cells that were obtained from both resting and exercised fasting subjects and stored for up to three days in ACD were unchanged as evaluated by both maximal specif...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03347594
更新日期:1983-08-01 00:00:00
abstract::EP1572 UMV1843 [Aib-DTrp-DgTrp-CHO]) is a new peptido-mimetic GH secretagogue (GHS) showing binding potency to the GHS-receptor in animal and human tissues similar to that of ghrelin and peptidyl GHS. EP1572 induces marked GH increase after s.c. administration in neonatal rats. Preliminary data in 2 normal young men s...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03345096
更新日期:2002-09-01 00:00:00
abstract::Orbital fibroblasts exhibit a unique phenotype including exaggerated responses to proinflammatory cytokines. We hypothesize that the unusual susceptability of these fibroblasts to molecular cues underlies the involvement of the orbit in Graves' ophthalmopathy. A number of attributes of orbital fibroblasts are reviewed...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:10.1007/BF03345273
更新日期:2004-03-01 00:00:00
abstract::We have measured eye muscle antibodies, in immunoblotting, in the serum from five patients with severe ophthalmopathy associated with Graves' hyperthyroidism who underwent plasmapheresis, correlating their levels with clinical features of the eye disorder and response to treatment. Blood taken before each plasma excha...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03348906
更新日期:1993-10-01 00:00:00
abstract:BACKGROUND:The aim of the study was to evaluate diurnal blood pressure (BP) profiles in patients with primary aldosteronism and to compare them to those in subjects with essential hypertension. The effects of specific therapy on the circadian BP profiles have been studied. MATERIALS AND METHODS:Sixty-four patients wit...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03349173
更新日期:2006-01-01 00:00:00
abstract:OBJECTIVE:We previously demonstrated that patients suffering from moderate hypothyroidism were at increased risk of thrombosis contrasting with the bleeding tendency of those presenting severe hypothyroidism. The latter state is associated with hemostatic anomalies including von Willebrand type 1 disease and increased ...
journal_title:Journal of endocrinological investigation
pub_type: 临床试验,杂志文章
doi:10.1007/BF03347217
更新日期:2005-05-01 00:00:00
abstract::Severe vitamin D deficiency has been found to be prevalent in institutionalized elderly persons in several countries. The aim of the present work was to assess the vitamin D status of institutionalized elderly and compare it to that of community-living independent elderly in southern Greece during summer. Serum 25-hyd...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03349258
更新日期:2008-09-01 00:00:00
abstract::Thyroid hormone acts on the oocytes, sperm and embryo during fertilization, implantation and placentation. Both hypothyroidism and hyperthyroidism may influence fertility. However, evidence of the association of hyperthyroidism with infertility is scarce and sometimes conflicting. Thyroid hormone influences human repr...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:10.1007/s40618-016-0452-6
更新日期:2016-08-01 00:00:00
abstract::A patient with Graves' disease associated with severe muscle weakness who was finally diagnosed as polymyositis by pathological examination of the muscle is reported. A 28-year-old women was incidentally found to have hyperthyroidism when she consulted a hospital for the evaluation and treatment of anemia in 1979. She...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03350077
更新日期:1989-12-01 00:00:00
abstract::We report an original application of quantitative reverse transcription-polymerase chain reaction (Q.RT-PCR) for the evaluation of atrial natriuretic factor (ANF), brain natriuretic peptide (BNP) and beta-actin mRNA in small atrial samples (10-15 mg). A fixed amount of total RNA was simultaneously reverse transcribed ...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03343655
更新日期:1999-12-01 00:00:00
abstract::Regulation of the hypothalamic-pituitary-thyroid (HPT) axis is dependent upon the secretion of thyrotropin-releasing hormone (TRH), a tripeptide originating in the hypothalamic paraventricular nucleus (PVN). These so-called hypophysiotropic neurons are under feedback inhibition by circulating levels of thyroid hormone...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:
更新日期:2004-01-01 00:00:00
abstract::Pituitary collision tumors are rare. They may create difficult diagnostic problems and their histogenesis is not clear. We report here an unusual case of a somatotroph adenoma colliding with a gonadotroph adenoma.The 64-year-old man had clinical acromegaly. His blood growth hormone level was elevated and magnetic reso...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03343674
更新日期:2000-01-01 00:00:00
abstract::We have developed a sandwich-type enzyme immunoassay for human chorionic gonadotropin (hCG), in which antibody Fab'-beta-D-galactosidase complex and an antibody-immobilized silicone rubber solid phase were used. Despite the fact this assay system crossreacted about 40% with human luteinizing hormone (LH) that contains...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03349444
更新日期:1981-07-01 00:00:00
abstract::Recombinant human thyroid-stimulating hormone (rhTSH) was developed to safely provide exogenous TSH stimulation in patients on thyroid hormone suppression therapy (THST), which is integral to long-term management of well-differentiated thyroid cancer. Such stimulation allows detection of thyroid remnant and neoplastic...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:
更新日期:1999-01-01 00:00:00
abstract::Thyroid diseases may lead to endothelial dysfunction; however, the mechanism underlying the endothelial dysfunction in thyroid disease is not clear yet. Asymmetric dimethylarginine (ADMA), a novel inhibitor of endothelial nitric oxide synthase (eNOS), blocks nitric oxide (NO) synthesis from L-arginine. Symmetric dimet...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03347423
更新日期:2007-03-01 00:00:00
abstract::Reduced cardiac mass and performances are present in GH deficiency and are counteracted by rhGH replacement. GH and IGF-I possess specific myocardial receptors and have been reported able to exert an acute inotropic effect. Synthetic GH secretagogues (GHS) possess specific pituitary and hypothalamic but even myocardia...
journal_title:Journal of endocrinological investigation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/BF03343555
更新日期:1999-04-01 00:00:00
abstract::Long-acting im bromocriptine was administered to 7 patients with pituitary macroadenomas (4 acromegalics, 1 Nelson's syndrome and 2 prolactinomas), with good tolerance except during the first 24 h. During a 42-day period hormonal, CT-scan and visual field variations were followed. In acromegalics HGH decrease was not ...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03348130
更新日期:1987-06-01 00:00:00
abstract::The aim of the study was to examine the effects of strenuous training on the hypothalamic- pituitary-adrenal axis activity. Exercise tests and saliva collections for analysis of awakening cortisol response (ACR) and midnight cortisol were performed before and after a 7-day period of intensified training in a group of ...
journal_title:Journal of endocrinological investigation
pub_type: 临床试验,杂志文章
doi:10.1007/BF03345561
更新日期:2008-01-01 00:00:00
abstract:PURPOSE:In patients with obesity, micronutrient deficiencies have been reported both before and after bariatric surgery (BS). Obesity is a chronic pro-inflammatory status, and inflammation increases the risk of micronutrient malnutrition. Our objective was to assess in pre-BS patients the prevalence of micronutrient de...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-020-01439-7
更新日期:2020-10-07 00:00:00