Abstract:
BACKGROUND:Zika virus is a mosquito-borne flavivirus responsible for recent outbreaks of epidemic proportions in Latin America. Sexual transmission of the virus has been reported in 13 countries and may be an important route of infection. Sexual transmission of ZIKV has mostly been male-to-female, and persistence of viral RNA in semen for up to 370 days has been recorded. The susceptibility to ZIKV of different testicular cell types merits investigation. METHODS:We infected primary Sertoli cells, a primary testicular fibroblast Hs1.Tes, and 2 seminoma cell lines SEM-1 and TCam-2 cells with ZIKV Paraiba and the prototype ZIKV MR766 to evaluate their susceptibility and to look for viral persistence. A human neuroblastoma cell line SK-N-SH served as a control cell type. RESULTS:Both virus strains were able to replicate in all cell lines tested, but ZIKV MR766 attained higher titers. Initiation of viral persistence by ZIKV Paraiba was observed in Sertoli, Hs1.Tes, SEM-1 and TCam-2 cells, but was of limited duration due to delayed cell death. ZIKV MR766 persisted only in Hs1.Tes and Sertoli cells, and persistence was also limited. In contrast, SK-N-SH cells were killed by both ZIKV MR766 and ZIKV Paraiba and persistence could not be established in these cells. CONCLUSIONS:ZIKV prototype strain MR766 and the clinically relevant Paraiba strain replicated in several testicular cell types. Persistence of ZIKV MR766 was only observed in Hs1.Tes and Sertoli cells, but the persistence did not last more than 3 or 4 passages, respectively. ZIKV Paraiba persisted in TCam-2, Hs1.Tes, Sertoli and SEM-1 cells for up to 5 passages, depending on cell type. TCam-2 cells appeared to clear persistent infection by ZIKV Paraiba.
journal_name
Virusesjournal_title
Virusesauthors
Mlera L,Bloom MEdoi
10.3390/v11010042subject
Has Abstractpub_date
2019-01-09 00:00:00issue
1issn
1999-4915pii
v11010042journal_volume
11pub_type
杂志文章相关文献
Viruses-Basel文献大全abstract::Internal ribosome entry is a key mechanism for viral protein synthesis in a subset of RNA viruses. Cricket paralysis virus (CrPV), a member of Dicistroviridae, has a positive-sense single strand RNA genome that contains two internal ribosome entry sites (IRES), a 5'untranslated region (5'UTR) and intergenic region (IG...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v8010025
更新日期:2016-01-19 00:00:00
abstract::Canine distemper virus (CDV) is a member of the genus morbillivirus, which is known to cause a variety of disorders in dogs including demyelinating leukoencephalitis (CDV-DL). In recent years, substantial progress in understanding the pathogenetic mechanisms of CDV-DL has been made. In vivo and in vitro investigations...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v6072571
更新日期:2014-07-02 00:00:00
abstract::Foot-and-mouth disease virus (FMDV) causes a highly contagious disease of cloven-hooved animals that poses a constant burden on farmers in endemic regions and threatens the livestock industries in disease-free countries. Despite the increased number of publicly available whole genome sequences, FMDV data are biased by...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v10040192
更新日期:2018-04-13 00:00:00
abstract::The Filoviridae family of viruses, which includes the genera Ebolavirus (EBOV) and Marburgvirus (MARV), causes severe and often times lethal hemorrhagic fever in humans. Filoviral infections are associated with ineffective innate antiviral responses as a result of virally encoded immune antagonists, which render the h...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v3091634
更新日期:2011-09-01 00:00:00
abstract::Dengue virus (DENV) infection has become a critically important globally prevalent infectious disease, especially in tropical and subtropical countries. Since neither currently exists, there is an urgent need for an effective vaccine to prevent, and a specific drug to treat DENV infection. Therapeutic peptides represe...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12111267
更新日期:2020-11-06 00:00:00
abstract::Hantaviruses are an established cause of haemorrhagic fever with renal syndrome (HFRS) in Europe. Following a confirmed case of HFRS in the UK, in an individual residing on a farm in North Yorkshire and the Humber, a tidal estuary on the east coast of Northern England, and the subsequent isolation of a Seoul hantaviru...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v6020524
更新日期:2014-02-05 00:00:00
abstract::West Nile virus (WNV) is prevalent in Africa, Europe, the Middle East, West Asia, and North America, and causes epidemic encephalitis. To date, no effective therapy for WNV infection has been developed; therefore, there is urgent need to find an efficient method to prevent WNV disease. In this study, we prepared and e...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v8120332
更新日期:2016-12-18 00:00:00
abstract::Human herpesvirus-6A and B (HHV-6A, HHV-6B) have recently defined endogenous genomes, resulting from integration into the germline: chromosomally-integrated "CiHHV-6A/B". These affect approximately 1.0% of human populations, giving potential for virus gene expression in every cell. We previously showed that CiHHV-6A w...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v8010019
更新日期:2016-01-15 00:00:00
abstract::Extensive surveillance in bat populations in response to recent emerging diseases has revealed that this group of mammals acts as a reservoir for a large range of viruses. However, the oldest known association between a zoonotic virus and a bat is that between rabies virus and the vampire bat. Vampire bats are only fo...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v6051911
更新日期:2014-04-29 00:00:00
abstract::Influenza A viruses (IAV) carrying reporter genes provide a powerful tool to study viral infection and pathogenesis in vivo, however, incorporating a non-essential gene into the IAV genome often results in virus attenuation and genetic instability. Very few studies have systematically compared different reporter IAVs,...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v10120679
更新日期:2018-11-30 00:00:00
abstract::The human cytomegalovirus (HCMV) terminase complex is part of DNA-packaging machinery that delivers a unit-length genome into a procapsid. Sequence comparison of herpesvirus homologs allowed us to identify a potential LATLNDIERFL and zinc finger pattern in N-terminal part of pUL56. Recombinant viruses were generated w...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11121093
更新日期:2019-11-26 00:00:00
abstract::Cellular receptor-mediated signaling pathways play critical roles during the initial immune response to Human Cytomegalovirus (HCMV) infection. However, the involvement of type-I transmembrane glycoprotein CD147/EMMPRIN (extracellular matrix metalloproteinase inducer) in the antiviral response to HCMV infection is sti...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v9120365
更新日期:2017-12-01 00:00:00
abstract::Under physiological conditions, the function of astrocytes in providing brain metabolic support is compromised under pathophysiological conditions caused by human immunodeficiency virus (HIV) and opioids. Herein, we examined the role of autophagy, a lysosomal degradation pathway important for cellular homeostasis and ...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v9080201
更新日期:2017-07-28 00:00:00
abstract::RNA viruses are extraordinary evolution machines that efficiently ensure their replication by taking advantage of the association with viral and cellular components to form ribonucleic complexes (vRNPs) [...]. ...
journal_title:Viruses
pub_type: 社论
doi:10.3390/v12121355
更新日期:2020-11-26 00:00:00
abstract::Influenza virus is among the most common causes of respiratory illness worldwide and can be complicated by secondary bacterial pneumonia, a frequent cause of mortality. When influenza virus infects the lung, the innate immune response is activated, and interferons and inflammatory mediators are released. This "cytokin...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11060505
更新日期:2019-06-01 00:00:00
abstract::Abstract: Since 2007, highly pathogenic clade 2.3.2 H5N1 avian influenza A (A(H5N1)) viruses have evolved to clade 2.3.2.1a, b, and c; currently only 2.3.2.1c A(H5N1) viruses circulate in wild birds and poultry. During antigenic evolution, clade 2.3.2.1a and c A(H5N1) viruses acquired both S144N and V223I mutations ar...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11100923
更新日期:2019-10-09 00:00:00
abstract::Laboratories throughout the world that perform work with Risk Group 4 Pathogens generally adopt one of two approaches within BSL-4 environments: either the use of positive pressure air-fed suits or using Class III microbiological safety cabinets and isolators for animal work. Within the UK at present, all laboratories...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v4081202
更新日期:2012-08-01 00:00:00
abstract::Severe fever with thrombocytopenia syndrome (SFTS), a tick-borne infectious disease, is difficult to differentiate from other common febrile diseases. Clinically distinctive features and climate variates associated with tick growth can be useful predictors for SFTS. This retrospective study (2013-2019) demonstrated th...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v13010010
更新日期:2020-12-23 00:00:00
abstract::Virus diseases greatly affect oilseed rape (Brassica napus) production. Investigating antiviral genes may lead to the development of disease-resistant varieties of oilseed rape. In this study, we examined the effects of the suppressor of gene silencing 3 in Brassica napus (BnSGS3, a putative antiviral gene) with diffe...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v7082815
更新日期:2015-07-27 00:00:00
abstract::Iminosugars that are competitive inhibitors of endoplasmic reticulum (ER) α-glucosidases have been demonstrated to have antiviral activity against a diverse set of viruses. A novel iminosugar, UV-4B, has recently been shown to provide protection against lethal infections with dengue and influenza A (H1N1) viruses in m...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v8030071
更新日期:2016-03-07 00:00:00
abstract::Different developmental stages of Artemia spp. (metanauplii, juveniles and adults) were bath-challenged with two isolates of the Lymphocystis disease virus (LCDV), namely, LCDV SA25 (belonging to the species Lymphocystis disease virus 3) and ATCC VR-342 (an unclassified member of the genus Lymphocystivirus). Viral qua...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11060506
更新日期:2019-06-01 00:00:00
abstract::In Europe, cowpox virus (CPXV) infection in South American camelids occurs as a so-called spill-over infection. Although infected animals generally have a mild form of the disease and survive, cases of fatal generalised CPXV infection have also been described. Prevention by prophylactic vaccination is the only way to ...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12020234
更新日期:2020-02-20 00:00:00
abstract::Delivering rapid protection against infectious agents to non-immune populations is a formidable public health challenge. Although passive immunotherapy is a fast and effective method of protection, large-scale production and administration of monoclonal antibodies (mAbs) is expensive and unpractical. Viral vector-medi...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12121460
更新日期:2020-12-17 00:00:00
abstract::Usutu virus (USUV) is a neurotropic flavivirus closely related to West Nile virus (WNV). Its enzootic cycle mainly involves mosquitoes and birds. Human infection can occur with occasional, but sometimes severe, neurological complications. Since its emergence and spread in Europe over the last two decades, USUV has bee...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12020164
更新日期:2020-01-31 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) establishes latency in resting memory CD4+ T cells and cells of myeloid lineage. In contrast to the T cells, cells of myeloid lineage are resistant to the HIV-1 induced cytopathic effect. Cells of myeloid lineage including macrophages are present in anatomical sanctuaries ma...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v7041578
更新日期:2015-03-31 00:00:00
abstract::Lettuce is often implicated in human norovirus (HuNoV) foodborne outbreaks. We identified H-like histo-blood group antigens (HBGAs) on lettuce leaves as specific binding moieties for virus-like particles (VLPs) of HuNoV GII.4/HS194/2009 strain. The objective of this study was to determine whether HuNoV-lettuce binding...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11090833
更新日期:2019-09-08 00:00:00
abstract::Decay accelerating factor (DAF/CD55) is targeted by many pathogens for cell entry. It has been implicated as a co-receptor for hantaviruses. To examine the binding of hantaviruses to DAF, we describe the use of Protein G beads for binding human IgG Fc domain-functionalized DAF ((DAF)₂-Fc). When mixed with Protein G be...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v6031091
更新日期:2014-03-10 00:00:00
abstract::West Nile virus (WNV) is an important emerging neurotropic virus, responsible for increasingly severe encephalitis outbreaks in humans and horses worldwide. However, the mechanism by which the virus gains entry to the brain (neuroinvasion) remains poorly understood. Hypotheses of hematogenous and transneural entry hav...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v6072796
更新日期:2014-07-18 00:00:00
abstract::Porcine circovirus 2 (PCV2) is the etiological agent that causes porcine circovirus diseases and porcine circovirus-associated diseases (PCVD/PCVAD), which are present in every major swine-producing country in the world. PCV2 infections may downregulate the host immune system and enhance the infection and replication ...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v11020185
更新日期:2019-02-21 00:00:00
abstract::Bacteriophage (phage) therapy is a promising alternative to antibiotics for the treatment of bacterial pathogens, including Clostridiumdifficile. However, as for many species, in C. difficile the physical interactions between phages and bacterial cells have not been studied in detail. The initial interaction, known as...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v10080411
更新日期:2018-08-06 00:00:00