Abstract:
Background:New drugs for adrenocortical carcinoma (ACC) are needed because most patients undergo rapid disease progression despite surgery and adjuvant therapy with mitotane. In this study, we aimed to investigate the in vitro effects of different chemotherapy drugs, alone or combined with mitotane, on the viability of adrenocortical carcinoma cells. Methods:Everolimus, sunitinib, zoledronic acid, imatinib and nilotinib cytotoxicity, alone or combined with mitotane were tested on ACC H295R cells in monolayer or spheroid cultures using MTS assays and confocal microscopy. Moreover, the nilotinib effects were investigated in spheroids cultured from patient tumor-derived ACC-T36 cells. Results:Morphological characterization of H295R cell spheroids using histochemistry was performed and showed that dense, homogenously sized, multicellular spheroids were obtained. We observed that sunitinib and nilotinib alone were equally effective in a monolayer preparation, whereas mitotane was the most effective even at a low dose. A combination of sunitinib and mitotane was the most effective treatment, with only 23.8% of cells in the monolayer remaining viable. Spheroid preparations showed resistance to different drugs, although the poor effect produced by mitotane alone was surprising, with a cell viability of 84.6% in comparison with 13.1% in monolayer cells. The most ineffective drugs in spheroid preparations were everolimus, zoledronic acid and imatinib. In both cell types, nilotinib, either alone or in combination with mitotane induced more significant cell viability inhibition in monolayer and spheroid preparations. In addition, the mechanism of nilotinib activity involves the ERK1/2 pathway. Conclusion:Taken together, our data identified nilotinib as a cytotoxic drug that combined with ERK inhibitors deserves to be tested as a novel therapy for adrenocortical carcinoma.
journal_name
Cancer Cell Intjournal_title
Cancer cell internationalauthors
Silveira E,Cavalcante IP,Kremer JL,de Mendonça POR,Lotfi CFPdoi
10.1186/s12935-018-0527-xsubject
Has Abstractpub_date
2018-03-01 00:00:00pages
29issn
1475-2867pii
527journal_volume
18pub_type
杂志文章abstract:Background:Breast cancer is one of the most common malignancy among females from the worldwide cancer incidence statistics. Peroxisome gamma coactivator-1β (PGC-1β) has long been identified to be involved in this type of tumorigenesis. However, the mechanisms of PGC-1β in human breast cancer have not been fully underst...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0810-5
更新日期:2019-04-11 00:00:00
abstract:BACKGROUND:CircRNAs play crucial roles in multiple tumours. However, the functions of most circRNAs in cervical cancer remain unclear. METHODS:This study collected GSE113696 data from the GEO database to search for differentially expressed circRNAs in cervical cancer. Quantitative reverse transcription PCR was used to...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01736-4
更新日期:2021-01-07 00:00:00
abstract:BACKGROUND:LncRNA HOXA-AS2 has been found in the literature to deteriorate glioblastoma. However, its regulatory mechanism is yet to be fully investigated. Our study focused chiefly on the interaction and role of the HOXA-AS2/miR-885-5p/RBBP4 axis in the development of glioblastoma. METHODS:qRT-PCR analysis was perfor...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01690-1
更新日期:2021-01-11 00:00:00
abstract:Objective:To study the potential role of miR-544 in the immune escape mechanism of hepatoma cells. Methods:Natural killer (NK) cells were collected from healthy volunteers and patients with liver cancer. Interleukin (IL)-2 activated-NK-92 cells were transfected with miR-544 inhibitor/mimic or NC/pre-NC in HepG2 co-cul...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-018-0542-y
更新日期:2018-04-03 00:00:00
abstract:Background:Many epidemiological studies have investigated association of AXIN2 variants on overall cancer risks; however, the available results remain inconsistent. Methods:An updated analysis was conducted to ascertain a more accurate estimation of the correlation between AXIN2 148 C/T, 1365 C/T, and rs4791171 A/G po...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0840-z
更新日期:2019-05-06 00:00:00
abstract:BACKGROUND:Epithelial mesenchymal transition (EMT) mediated by TGF-β pays an important role in malignant tumor acquired abilities of migration and invasion. Our previous study showed that the extract of Stellera chamaejasme L. (ESC) was against proliferation of a variety of tumor cells, but there were no studies in the...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-015-0265-2
更新日期:2015-12-10 00:00:00
abstract:Background:Lung cancer is the most common malignant tumor in the world. The Whole-proteome microarray showed that ubiquitin ligase chromatin assembly factor 1 subunit B (CHAF1B) expression in A549/DDP cells is higher than in A549 cells. Our study explored the molecular mechanism of CHAF1B affecting cisplatin resistance...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01263-2
更新日期:2020-05-25 00:00:00
abstract:Background:Dysregulation of lncRNAs is frequent in glioma and has emerged as an important mechanism involved in tumorigenesis. Previous analysis of Chinese Glioma Genome Atlas (CGGA) database indicated that LBX2-AS1 expression is one of differentially expression lncRNA between lower grade glioma (LGG) (grade II and III...
journal_title:Cancer cell international
pub_type: 杂志文章,收录出版
doi:10.1186/s12935-020-01433-2
更新日期:2020-08-26 00:00:00
abstract:: Autoimmune phenomena were identified in many different cases of hematological diseases and solid tumors, which may be due to alterations in the expression and function of the NF-κB signaling pathway. Recently, a number of studies have shown that the deletion or mutation of A20, a negative regulator of NF-κB, is frequ...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-12-44
更新日期:2012-11-07 00:00:00
abstract:BACKGROUND:The thieno[2,3-b]pyridines were discovered by virtual high throughput screening as potential inhibitors of phospholipase C (PLC) isoforms and showed potent growth inhibitory effects in National Cancer Institute's human tumour cell line panel (NCI60). The mechanism of the anti-proliferative activity of thieno...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-016-0293-6
更新日期:2016-03-10 00:00:00
abstract:Background:Osteosarcoma (OS) is still a disease with high mortality from malignant tumors in children and adolescents. Due to its poor treatment, this study explored the involvement of lncRNA ZFAS1/microRNA-135a (miR-135a)/apurinic/apyrimidinic exonuclease 1 (APEX1) axis in the regulation of OS growth and metastasis. ...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-1049-x
更新日期:2019-12-03 00:00:00
abstract:Background:Renal cell carcinoma (RCC) is the most common kidney cancer and includes several molecular and histological subtypes with different clinical characteristics. The combination of DNA methylation and gene expression data can improve the classification of tumor heterogeneity, by incorporating differences at the ...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0900-4
更新日期:2019-07-16 00:00:00
abstract:BACKGROUND:Prostate cancer progression to androgen independence is the primary cause of mortality by this tumor type. The IGF-1/IGF-1R axis is well known to contribute to prostate cancer initiation, but its contribution to invasiveness and the downstream signalling mechanisms that are involved are unclear at present. ...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-8-10
更新日期:2008-07-03 00:00:00
abstract:BACKGROUND:Advanced cancer and chemotherapy are both associated with immune system suppression. We initiated a clinical trial in patients receiving chemotherapy for metastatic colorectal cancer to determine if administration of GM-CSF in this setting was immunostimulatory. METHODS:Between June, 2003 and January, 2007,...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-12-2
更新日期:2012-01-23 00:00:00
abstract:Background:Immunotherapies that targeting programmed cell death 1 (PD-1) and programmed death-ligand 1 (PD-L1) have obtained prominent success in breast cancer (BC). However, not all the patients benefit from the antibody therapy. This study aimed to identify PD-1/PD-L1 correlated genes and pathways as well as investig...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0955-2
更新日期:2019-09-09 00:00:00
abstract:Background:Casticin, an isoflavone compound extracted from the herb Fructus Viticis, has demonstrated anti-inflammatory and anticancer activities and properties. The aim of this study was to investigate the effects and mechanisms of casticin in nasopharyngeal carcinoma (NPC) cells and to determine its potential for tar...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-1069-6
更新日期:2019-12-21 00:00:00
abstract::[This corrects the article DOI: 10.1186/s12935-019-0742-0.]. ...
journal_title:Cancer cell international
pub_type: 杂志文章,已发布勘误
doi:10.1186/s12935-019-0797-y
更新日期:2019-04-11 00:00:00
abstract:Background:Cholangiocarcinoma (CCA) is an invasive malignancy arising from biliary epithelial cells; it is the most common primary tumour of the bile tract and has a poor prognosis. The aim of this study was to screen prognostic biomarkers for CCA by integrated multiomics analysis. Methods:The GSE32225 dataset was der...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01212-z
更新日期:2020-04-22 00:00:00
abstract:Background:Long noncoding RNAs (lncRNAs) are involved in the tumorigenesis and progression of human cancers, including renal cell carcinoma (RCC). Small nucleolar RNA host gene 4 (SNHG4) is reported to play an essential role in tumor growth and progression. However, the molecular mechanisms and function of SNHG4 in RCC...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01606-z
更新日期:2020-10-19 00:00:00
abstract:Background:Colorectal cancer (CRC) is considered as the second common death-induced cancer. More recently, association of long non-coding RNAs (lncRNAs) with CRC has been extensively investigated. Therefore, the present study was performed to determine whether lncRNA MAF BZIP Transcription Factor G Antisense RNA 1 (MAF...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01485-4
更新日期:2020-10-19 00:00:00
abstract:BACKGROUND:Hepatocellular carcinoma is a common cancer, ranking third in cancer-associated deaths. An important cause of cancer patients' mortality is metastasis. At the start of metastasis progression, there is an epithelial-mesenchymal transition, characterized by matrix degradation, junction reductions and vessels f...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-016-0370-x
更新日期:2017-01-04 00:00:00
abstract:BACKGROUND:Natural killer (NK) cells eliminate virus-infected and tumor cells through the release of perforins and granzymes; they also produce Interferon gamma (IFN-γ) and Tumor necrosis factor alpha (TNF-α), which induce apoptosis in target cells. Many tumors express Heme oxygenase 1 (HO-1), and this expression has b...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-014-0100-1
更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:We have previously shown that ultraviolet-A (UVA) radiation enhances metastatic lung colonization capacity of B16-F1 melanoma cells. The aim of this study was to examine changes in expression profile of genes in mouse melanoma B16-F1 cells exposed to UVA radiation. RESULTS:B16-F1 melanoma cells were exposed...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-7-7
更新日期:2007-05-02 00:00:00
abstract:Background:Chimeric antigen receptors (CARs) represent a novel facet of modern day synthetic biology that exemplifies personalized medicine at work through their ability to harness and redirect a patient's immune system to fight cancer. Body:By combining the target-specificity of antibodies to the effector capabilitie...
journal_title:Cancer cell international
pub_type: 杂志文章,评审
doi:10.1186/s12935-018-0685-x
更新日期:2018-11-14 00:00:00
abstract:BACKGROUND:Several reports have revealed that cancer stem cells (CSCs) exist in many types of solid tumors. Some studies have demonstrated that side population (SP) cells isolated from diverse cancer lines harbor cancer stem-like properties, but there are few reports examining the characteristic of SP cells in human ce...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-14-3
更新日期:2014-01-13 00:00:00
abstract:BACKGROUND:Abnormal proliferation, metastasis and epithelial-mesenchymal transformation (EMT) of lens epithelial cells (LECs) are direct factors of posterior capsular opacification (PCO). Nuclear enriched abundant transcript 1 (NEAT1) has been shown to promote cell proliferation, metastasis and EMT, but whether it affe...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01619-8
更新日期:2020-10-31 00:00:00
abstract:BACKGROUND:ErbB3-binding protein 1 (Ebp1) is a member of the PA2G4 family of proliferation-regulated proteins that is expressed in multiple malignant and non-malignant cells. ErbB3 and other members of the EGFR family have been implicated in cancer progression, it however remains unknown whether Ebp1 participate in pro...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-8-18
更新日期:2008-11-24 00:00:00
abstract:BACKGROUND:Phenethyl isothiocyanate (PEITC), present naturally in cruciferous vegetables, is a chemopreventive agent. It blocks initiation and post-initiation progression of carcinogenesis. Mechanism study in human prostate cancer cells revealed that PEITC is a dual inhibitor of aberrant DNA hypermethylation and histon...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-14-30
更新日期:2014-03-31 00:00:00
abstract:Background:Accumulating studies showed that long noncoding RNAs (lncRNAs) played vital roles in cancer progression. LncRNA MIR4435-2HG was proved to act as an oncogene in various tumors. However, the underlying function of MIR4435-2HG in ovarian cancer (OC) remains unclear. Methods:The expression levels of MIR4435-2HG...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01227-6
更新日期:2020-05-01 00:00:00
abstract::RNA methylation has emerged as a fundamental process in epigenetic regulation. Accumulating evidences indicate that RNA methylation is essential for many biological functions, and its dysregulation is associated with human cancer progression, particularly in gastrointestinal cancers. RNA methylation has a variety of b...
journal_title:Cancer cell international
pub_type: 杂志文章,评审
doi:10.1186/s12935-020-01679-w
更新日期:2020-12-07 00:00:00